A guide on how to use CRISPR RNP for targeted genome editing.

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What is included
  • How to prepare RNA oligos
  • How to prepare RNP complex
  • How to test RNP efficacy in cells, through either cationic lipid-mediated transfection or electroporation
  • How to test RNP efficacy in embryos
  • How to test RNP in vitro cutting efficiency
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