Overview

GenScript licensed CRISPR/Cas9 technology from the Broad Institute of MIT and Harvard, and provides full CRISPR-based gene editing service to produce genetically modified cells lines using any mammalian cell line and targeting any gene.

Services

Service Name Service Details Cell Line Options * Deliverables Price Delivery Time
EZ Knockout Cell Line Service
Low Price!
Single gene knockout cell line
CRISPR Ribonucleoprotein-based editing
Customer specifies gene/locus region and cell line of interest*
Available for 90+ popular transfection-suitable cell lines, including A549, CHO-K1, HEK293, HEK293T, HT-29, MDA-MB-231, 4T1, A20, HCT116, MCF7, MDCK, U937, RPMI 8866, etc.
  • Two full-allelic knockout cell lines validated by sequencing
  • One negative knockout cell line as control
  • Biweekly project updates

**Validation of full-allelic knockout cell lines at mRNA or protein level upon request

$7,000 9-15 weeks depending on project complexity and cell line difficulty
Customized Knockout Cell Line Service
Low Price!
Single genes editing knockout cell line
CRISPR Ribonucleoprotein-based editing
Customer specifies gene/locus region and cell line of interest*
Any immortalized cell line
  • 1-2 full-allelic knockout cell line(s) validated by sequencing
  • One negative knockout cell line as a control
  • Biweekly project updates

**Validation of full-allelic knockout cell lines at mRNA or protein level upon request

$9,000 16-24 weeks depending on project complexity and cell line difficulty
Knockout Cell Pool Service Single gene knockout stable cell pool
Lentivirus-based
Customer specifies gene/locus region and cell line of interest*
Any cell line
  • Stable cell pool containing knockout clones validated by sequencing
  • Biweekly project updates

**Validation of full-allelic knockout cell lines at mRNA or protein level upon request

Starting from
$6,800
$4,800
5-11 weeks depending on cell line difficulty

* It is preferred that customers provide their own cell lines, despite available cell lines can be purchased by GenScript for an additional fee. Please note that we currently do not provide genome editing service for primary cells, stem cells or iPS cells.

** Validation services for knock-out cell lines can be found under the "Add On Services" tab.

CRISPR Workflow

CRISPR gene editing workflow

Add-On Services

Optional Services

Add-On Service Description
Reverse transcription (RT)-PCR Validate the knockout clones to carry the INDELs on CDS at mRNA level by sequencing the RT-PCR product. 
Western blot Validate the knockout clones by western blot. A validated antibody in the host cells with specific binding to target protein (RNAi or knockout) must be provided.
FACS analysis Validate the knockout clones by FACS analysis. A validated antibody in the host cells with specific binding to target protein (RNAi or knockout) must be provided. 
Off-target analysis Characterize one knockout clone by sequencing the top 10 of potential off-target sites. 
One additional clone Sequence additional single clones to identify one additional knockout clone. QC and deliver one additional knockout clone.

Case Studies

Get in Touch
with GenScript Custom
CRISPR Experts

Get in Touch with GenScript Custom CRISPR Experts
TurboCHO High Performance Services
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Customer Reviews

17 Customer Reviews

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