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CRISPR gene editing service

CRISPR Gene Editing Services

CRISPR genome editing service

CRISPR is revolutionizing the field of gene editing. It has the potential to alter the course of research and drug discovery, by providing scientists with a powerful tool to change any gene, in any cell in a highly targeted manner and without introducing foreign DNA. The benefits of CRISPR/Cas9 over previous forms of gene editing, such as TALENs and zinc finger nuclease (ZFN), are that it is much simpler to implement and has higher efficiency at performing bi-allelic gene modifications.

GenScript is pleased to introduce GenCRISPR™, a full CRISPR-based gene editing service, to produce a genetically modified cell using any mammalian cell line and targeting any gene.  Our scientists are experts at performing gene editing with CRISPR, from designing gRNA constructs for CRISPR to transfection and single clone generation of a wide range of cells, including difficult-to-transfect and tumor cell lines.

GenCRISPR™ cell line service Cell Lines Timeline Starting Price^ Deliverables and Details

EZ Knock-out cell line service
(SC1755)

  • Single gene editing KO cell line
  • Customer specifies gene/locus region and cell line of interest*
  • Transfection-based
  • Validated gRNA-Cas9 constructs
30 easy-to-handle cell lines 13-16 weeks $6,000
  • 2 full-allelic knock-out cell lines validated by sequencing
  • 2 cryovials of cells per each clone, mycoplasma-free and 90% viability.
  • 1 negative control cell line*
  • Biweekly update of project progress

***Validation of full-allelic knock-out cell lines at mRNA or protein level upon request

100 medium difficulty cell lines 13-16 weeks $7,000

Customized Knock-out cell line service
(SC1652)

  • Single or multiplex genes editing KO cell line
  • Customer specifies gene/locus region and cell line of interest**
  • Transfection based
  • Validated gRNA-Cas9 constructs
  • Cas9 stable cell pool service available for difficult cell lines (SC 1758)
Any cancer or immortalized cell line of interest

16-20 weeks
(easy-to-handle lines)

20-26 weeks
(difficult-to-handle lines)

$8,000
  • *1-2 full-allelic knock-out cell line(s) validated by sequencing
  • 2 cryovials of cells per each clone, mycoplasma-free and 90% viability.
  • 1 negative control cell line*
  • Biweekly update of project progress

***Validation of full-allelic knock-out cell lines at mRNA or protein level upon request

Lenti-CRISPR KO Service
(SC1652-V)

  • Single gene editing KO cell line
  • Customer specifies gene/locus region and cell line of interest**
  • Lentiviral based for difficult-to-transfect cell lines.
  • Cas9 stable cell pool service available for difficult cell lines (SC 1758)
Any cancer or immortalized cell line of interest 16-26 Weeks $10,000
  • 1 full-allelic knock-out cell line(s) validated by sequencing
  • 2 cryovials of cells per each clone, mycoplasma-free and 90% viability
  • Cas9-stable cell pool as negative control
  • Biweekly update of project progress

***Validation of full-allelic knock-out cell lines at mRNA or protein level upon request

*It is preferred that customers provide their own cell lines, but cell lines can also be purchased by GenScript for an additional fee.

**Prices may vary based on project specifications

***Validation services for knock-out cell lines can be found under the "Add On Services" tab.

CRISPR workflow

CRISPR gene editing workflow

Optional Services

Add-On Service Description
Reverse transcription (RT)-PCR Validate the knockout clones to carry the INDELs on CDS at mRNA level by sequencing the RT-PCR product. 
Western blot Validate the knockout clones by western blot. A validated antibody in the host cells with specific binding to target protein (RNAi or knockout) must be provided.
FACS analysis Validate the knockout clones by FACS analysis. A validated antibody in the host cells with specific binding to target protein (RNAi or knockout) must be provided. 
Promoter activity survey Survey the promoter activity (Cbh/CMV/EFS) in the host cells to maximize gRNA-Cas9 cleavage efficiency. 
Transfection optimization Survey the transfection methods for the hard-to-transfect cells to maximize gRNA-Cas9 cleavage efficiency.
Off-target analysis Characterize one knockout clone by sequencing the top 10 of potential off-target sites. 
One additional clone Sequence additional single clones to identify one additional knockout clone. QC and deliver one additional knockout clone.

Related services

CRISPR genome editing service

Cell Lines Available for EZ KO Cell Line Service

Easy-to-handle and difficult-to-handle cell lines are categorized based on their transfection ability. As compared to Easy-to-handle cell lines, difficult-to-handle cell lines need additional characterization methods including but not limited to: transfection optimization, promoter activity testing, clonality optimization, cell culture establishment, etc.

Easy-To-Handle
Cell Lines
Difficult-To-Handle Cell Lines
A2058 HT-1080 2PK3 Capan2 HACAT KG-1a MOLT-4 RFL-6 T98G
A-375 Jurkat E6.1 4T1 CCRF-CEM HCT 15 L6 N-2a (Neuro-2a) RS4-11 U138MG
A-498  MCF7 786-0 Colo201 HEL 92.1.7 LN229 NCI-H1299 Saos-2 U266B1
A549 mIMCD3 A20 COS-7 HeLa S3 LNCaP
clone FGC
NCI-H2170 SH-SY5Y U87 MG
Achn MRC-5 A673 D1 ORL UVA Hepa 1-6 MA-104
Clone 1
NCI-H292 SK-BR-3 U-937
B16-F10 NCI-H226 AGS DB  HepG2 MC57G NCI-H358 SK-MEL-28 V79-4
BT549 NCI-H23 Alpha T3 EAhy926 HK2 MDA-MB-231 NCTC clone 929 SK-N-SH WEHI-279
CHO-K1 NCI-H441 ARPE-19 EL4 HL-60 MDA-MB-435 OVCAR3 SK-OV-3 WERI-Rb-1
Colo320 NCI-H460 B16-F0 F9 HT-29 MDA-MB-453 P815 Sp2/0-Ag14 Wit49
DLD-1 NIH/3T3 B16-F1 FAO Huh-7 MDA-MB-468 PANC-1 SUP-T1 WSU-DLCL2
HCT 116 PC-3 BHK-21 G-361 HuT 78 MEG-01 PC-12 SW480  
HEK293 RKO C6 GH3 J774A.1 MeWo PDFS SW620  
HeLa U2OS CA46 H4 JeKo-1 MG-63 RBL-1 SW837  
Hep 3B Vero76 Caco-2 H69 K562 MiaPaCa RD T24  
Hep-2   Calu-6 H9C2 KG-1 MM.1S REH T-47D  

Quotations and Ordering

CRISPR genome editing service

For quotation requests:

To place an order:

Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you. However, you may also contact us by email, phone, or fax.

 
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