Patent Number: WO2020024917A1

As a leading provider of synthetic DNA, GenScript is dedicated to using the latest advancements to update existing algorithms for improving gene expression. Our most recent development is GenSmart™ Codon Optimization; a free, user-friendly online tool that enables you to optimize the design of wild type or recombinant gene sequences towards higher expression in prokaryotic and mammalian expression systems.

GenSmart™ Codon Optimization uses a newly-designed algorithm based on the "Population Immune Algorithm", which takes advantage of both population genetics and immunology theories. In this approach, more than 200 factors involved in gene expression, including GC content, codon usage and content index, RNase splicing sites, and cis-acting mRNA destabilizing motifs are screened and validated. Instead of applying a single-factor simulation to computing, a multifactor approach is employed to ensure that all key factors in a certain target gene sequence carry weight. As a result, each gene optimization is fully customized to maximize the chance of obtaining a functional and active protein.

Compared to similar tools, GenSmart™ Codon Optimization is more accessible and user-friendly. All of the parameters are integrated into the algorithm and require no input from you. All that is required is your sequence and desired host organism; the rest is taken care of by the optimization tool. In addition, your optimized sequences can be easily ordered through GenSmart™ Instant Quote System or archived for future use.

Upgraded! GenSmart™ Codon Optimization has been applied to over 50 host organisms now, including CHO, Human, E.coli, T cell, Yeast, Insect, Arabidopsis, Maize, Rice, Soybean, Fruit fly, etc.!

Why GenSmart™ Codon Optimization


Free, online tool; just one click away to your optimal sequence

Comprehensive Factor Analysis

Over 200 factors screened and validated

Advanced Computing

Patented population immune algorithm to ensure the best output

Individual Sequence-Based

Highly customized computing to avoid weight allocation bias on key factors

Easy Ordering

Seamless integration with GenSmart™ Instant Quote System

What the Data Say

Related Tools and Resources

Rare Codon Analysis Tool

A powerful online tool for analyzing codon usage frequency and codon usage distribution of given sequences.

Learn More
Commonly Used Codon Frequency Tables

Commonly used genetic codon frequency tables in different host organisms.

Learn More
The Genetic Code

A Universal Template for Protein Translation.

Learn More



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