| QC Items | Specification |
|---|---|
| Appearance | Clear, no visible particles |
| A260/280 | 1.8-2.0 |
| Supercoiled content | 90%±10% supercoiled* |
| Residual RNA | Not visible |
| Genomic DNA | Not visible** |
| Restriction Analysis | Conforms to reference |
| Endotoxin Assay (LAL test) | Endotoxin-level free (≤ 0.1 EU/μg) |
* Percentage of supercoiled plasmid may vary when analyzing with different methods or can be altered due to shipping conditions.
** Since densitometry is semi quantitative, the genomic DNA is considered nonvisible if the comparative intensity of genomic DNA < 15% of plasmid band. Quantitative genomic DNA measurement by qPCR is available with additional charges.
Questions about our quality control? Find out more here.
Fluorescence microscopy comparison of Industrial Grade plasmid prep transfection efficiency vs. HT plasmid prep transfection efficiencies in HEK293, Jurkat and Huh-7 cells. The levels of GFP fluorescence in HEK293 cells transfected with HT plasmid prep is at comparable levels to the HEK293 cells transfected with industrial grade plasmid prep. This indicates almost similar levels of transfection efficiencies between the two plasmid prep services.
The antibody expression yields of HT plasmid prep transfected HEK293 cells is comparable in relative levels to the industrial grade transfected HEK293 cells, further illustrating the comparable transfection efficiencies between the two plasmid prep services.
When requesting a quote for the HT plasmid DNA preparation service, please specify:
Quantity of plasmid DNA and # preps required: either 10 μg or 30 μg
Storage condition: TE buffer (liquid)
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