GenScript's Plasmid DNA Preparation Service is accommodating to both small research labs as well as large-scale manufacturing biotech and pharmaceutical companies, providing you with high quality plasmids with 100% full insert sequence accuracy. Choose from a variety of our packages to help you achieve highly efficient cell transfection, and to support your DNA vaccine development, antibody production, and other preclinical research projects. Request a quote now for New plasmids to be synthesized, or Re-prep of previously synthesized plasmid.
With close to 20 years of CRO experience, GenScript truly understands your needs. From transformation to extraction, we have developed a robust protocol with years of process optimization to provide you top-notch quality plasmid preps with fully customizable services.
Industrial Grade Plasmid DNA to deliver highly efficient cell transfection with highly homogenous (≥90±10% supercoiled) plasmids. 100% sequence accuracy guaranteed!
Preclinical grade plasmid DNA with the most comprehensive and stringent quality specifications. Highly scalable from 10mg and up. Enjoy customizable options like animal-free production, AAV-ITR service and add-on QC options.
| Research Grade | Industrial Grade | HT Grade | Pre-clinical Grade | |
|---|---|---|---|---|
| Price | From $39 | From $99 | From $29 | From $1199 |
| Volume | 100 µg-2 g | 100 µg-2 g | 10 µg-30 µg | 10 mg-2 g |
| Turnaround Time | From 2BD (bundled) From 8BD (stand alone) |
From 2BD (bundled) From 8BD (stand alone) |
From 2BD (bundled) From 9BD (stand alone) |
From 2BD (bundled) From 9BD (stand alone) |
| Homogeneity | Predominantly supercoiled | ≥90±10% supercoiled | ≥90±10% supercoiled | ≥90% supercoiled |
| Endotoxin | - | Quantitative LAL assay <0.01 EU/µg | Quantitative LAL assay <0.1 EU/µg | Quantitative LAL assay ≤0.01 EU/µg or ≤0.005 EU/µg |
| Residual RNA | Not visible on agarose gel | |||
| E.coli Genomic DNA | < 15% by agarose gel electrophoresis | ≤ 5% by qPCR | ||
| Residual Host Protein | - | HCP ELISA ≤1% | ||
| Bio-Burden Test | - | No viable microorganisms in /on culture media | ||
| Animal-free Production* | Available with additional charge | |||
| Enzyme Free Production | - | Available with additional charge | ||
| Advanced Endotoxin Removal <0.005 EU/µg | - | Available with additional charge | ||
| Mycoplasma Contamination | - | Available with additional charge | ||
| Material Archiving | - | Available with additional charge | ||
| Appearance | Colorless, clear, free of precipitate or foreign particles | |||
| A260:280 | 1.8 - 2.0 | |||
| pH | - | 8.0 ± 0.5 (in TE buffer) 7.4 ± 0.5 (in PBS buffer) (5-7) ± 0.5 (in ddH2O buffer) |
||
| Restriction Analysis | DNA bands with expected sizes detected by agarose gel electrophoresis | |||
Note:
*:For research, industrial and HT grade plasmid preps:
Since
densitometry is semi quantitative, the genomic DNA is considered nonvisible if the
comparative intensity of genomic DNA
< 15% of plasmid band. Quantitative genomic DNA measurement by qPCR is available with additional
charges.
**: Sequence verification of the full gene insert only
applies when bundled with gene synthesis (an insert to be cloned into a
plasmid).
***: Percentage of supercoiled plasmid may vary when analyzing
with
different methods or can be altered due to shipping conditions.
Additional QC available upon request
Learn more about GenScript’s proprietary AAV ITR Guarantee platform
Learn how improved ITR integrity can impact AAV plasmid expression and accelerate your gene therapy pipeline!
With the most
comprehensive and stringent QC
Tailored for preclinical studies
As a leading biotech company and the largest gene synthesis supplier in the USA,
GenScript
has accumulated a wealth of experience in GLP-level plasmid preparation.
We are
happy
to offer GLP plasmid prep services to provide you with ultra transparent documentation
system and communication.
Strict
Operating Procedurces
Comprehensive
Final Report
Data Archive
Management
Dr. Melissa A. Melan
University of Pittsburgh
Medical Center,
USA
We used GenScript's plasmid preparation service to prepare large quantities of plasmids that contain our Southern blot probe fragments. This saved our lab considerable time and expense. From the initial order to service delivery, GenScript provided our lab with timely follow-ups and valuable advice. We now have an adequate supply of these probes with no need to grow bacterial cultures every time a new probe is required. Thank you, GenScript!
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Gene Therapy Tidbits
Learn how improved ITR integrity can impact AAV plasmid expression and accelerate your gene therapy pipeline!
