GenScript's proprietary OptimumRNAi technology offers 2nd generation siRNA design tool, which can efficiently reduce off-target results and enhance the knockout rate.
Use this tool to identify candidate siRNA target sequences within your cDNA.
GenScript's siRNA design tool is effective and easy to handle. With the new tools, at least three out of four siRNA constructs will result in an mRNA knockdown rate of at least 75% where the transfection efficiency of the experiment is at least 80%.
The confidentiality of your sequence information is protected by Verisign Encryption Technology.
Use this tool to build small hairpin inserts. GenScript precision gives your project the highest possible knockdown efficiency.
GenScript siRNA Construct Builder queries and processes information from a variety of databases, including not only the public GenBank database but also GenScript's specialized proprietary expression vector, restriction endonuclease, and siRNA loop databases. The GenScript siRNA Construct Builder provides analysis and calculation results tailored to the user's specific requirements, providing four critical types of information: 1) Target siRNA: this is the sequence of sense siRNA, the sequence of the loop, and the sequence of antisense siRNA. 2) Custom cloning sites (restriction sites) 3) Description of the expression vector selected by the user 4) The necessary quantity of synthesized oligonucleotides.
This software creates a scrambled sequence as negative control for siRNA experiments.
The scrambled sequence will have the following features:
To identify a really good candidate, up to 10,000 scrambling combinations may be processed.
GenScript's Genome-Wide Bio-Reagent Bank (GenPool) includes 80,139 pre-designed siRNA designed for use with human, mouse, and rat genes. Our design saves time and maximizes the chance of a successful experiment. GenScript's siRNA design algorithm includes analysis for sequence specificity, thermodynamic properties, complexity, secondary structure, and SNPs.
