Gene Synthesis Services ^Upgraded

Service Details

Sequence Input	Length: Up to 200,000 bp Format: Nucleotide or Amino acid sequences
Cloning Options	Vectors: Choose from GenScript Clone Vectors, Expression Vectors, or your Vectors Cloning Methods: Choose from restriction cloning, or seamless cloning
Plasmid DNA Options (From 2 Business Days)	Quantity: Choose from 10µg to 2g Supercoil: Choose from predominantly supercoil, ≥ 80% supercoil, or ≥ 90% supercoil Endotoxin Level: Choose from ≤ 0.1 EU/µg, ≤ 0.01 EU/µg, ≤ 0.005 EU/µg, or not guarantee Poly A guarantee add-on service AAV ITR guarantee add-on service Optional quality control tests and production processes
Turnaround Time Options	From 72 hours The Fastest Rocket From 5 Business Days Most Popular Premium From 9 Business Days Best Value Basic

*If you need more than 4µg of plasmid DNA for your downstream application, order our bundled plasmid DNA preparation service and add just 2+ business days.

Tips to get your DNA synthesis projects faster

• Choose Rocket Gene Synthesis service to save 3 more days of production time
• Use GenScript’s default clone vector to save 2-5 days of production time

Please Note:

• Turnaround time and price for the gene synthesis projects are subject to change based on length, customizations, and complexity. The additional shipping time will differ depending on the destination country. Get your accurate estimated pricing and turnaround time with GenSmart™ 2.0 online ordering platform.
• Learn more about our GenBrick™ technologies for long gene synthesis. Contact our customer support team for accurate estimated pricing and turnaround time for your genes longer than 8,000 bp.
• General guidelines for DNA sequence difficulty
• Example NGS QC report for your gene order

Typically, the synthesis success rate is mainly determined by DNA structures including repetitive sequences and GC content, and the stability in host cells including the toxicity for specific host cells. To improve the synthesis success rate of your gene sequences, we have summarized the main factors that we take into account for sequence analysis.

The main factors are including:

• Global GC content less than 20% or higher than 80%
• Various GC content within a DNA sequence
• Homopolymers
• Repeats of ≥ 20 bp
• The number and the length of small repeats
• Palindromic sequence

With over 18 years experience on nucleotide synthesis, GenScript has fully developed state-of-the-art platform to handle both easy and difficult gene sequences. For the coding sequence, we strongly recommend codon optimization to reduce the difficulty. If you have any questions on our Gene Synthesis Services, please contact your technical account manager for details.

Deliverables Include

• 4 μg of lyophilized plasmid (high-copy) containing your gene insert or 1 μg of lyophilized plasmid (low-copy) containing your gene insert
• Sanger sequencing result covering your gene insert (electronic)
• Construct map for the plasmid (electronic)
• Certificate of analysis

Vector Selection Guide

Quote/Order Quote/Order

Global Production Network

Cutting-edge facilities in China, the United States, and Singapore offer top-quality gene synthesis, cloning, and plasmid DNA services to global researchers. Our worldwide presence ensures quick and efficient access to our top-notch services, enabling researchers to focus on their core work with confidence. Trust in our expertise and global reach to support your research needs and propel innovation in the field of genetic sciences.

China

The United States

Singapore

Resources

GenSmart™ 2.0 Online Ordering

4 steps to get your quote or order your clonal gene.

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GenSmart™ 2.0 Quick User Manual

Essential steps and tips to get familiar with GenSmart™ 2.0

Free Download

GenSmart™ Codon Optimization

A free online tool for codon optimization to improve gene expression and more.

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GenSmart™ Vector Design

A free online tool to design your own plasmid vectors.

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Testimonials

"I recently had the pleasure of using GenScript’s Gene Synthesis service. I was impressed by their Fair, Transparent Pricing and Exceptional Customer Service, which included prompt and clear communication. They delivered my genes on schedule, earning my trust and recommendation for anyone in need of Reliable gene synthesis services."

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Matt Wang

Trait Biosciences

"GenScript's services, including Gene Synthesis, Peptide Synthesis, and Antibody Production, have proven invaluable to my research. Their services are always of the Highest Quality with Reliable turnaround times. "

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Venkata Vamsee Mallajosyula

Stanford University

"GenScript simplified my Gene Synthesis order, quickly designing and creating exactly what I needed, even correcting my submission errors. Fast and reliable service! Expert staff!"

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Maranda McDonald

John Hopkins University

"GenScript has been my go-to for DNA Cloning, Mutagenesis, and Gene Synthesis services. Consistently over the past several years, with hundreds constructs ordered, I have received excellent customer service, pricing, and speedy delivery without any letdowns."

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Christopher Lennon

Murray State University

"Thanks to GenScript, I was able to generate a CAR-T cell product with high transduction efficiency and efficacy. I'm glad to have GenScript's help with gene synthesis and plasmid preparation in my workflow, and I plan to keep using their services for my future projects, even after my PhD. They are extremely communicative and thorough at every stage of my project without even having to ask, and their transparency and pleasantness is why I continue to come back to GenScript"

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Samantha Chin

Graduate Student, The George Washington University

"From 2009, till today I was with GenScript. I never face any challenge. very easy and commendably service I received from here. so many time I have worked with difficult to expressed gene. Thanks to GenScript for each and every solution. I am happy to work with you for many more years. "

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Parvinder Kaur

Senior Manager, Meril Medical Innovation