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GenScript Target Enrichment Solutions

As whole genome NGS always leads to high cost, long experiment time and large data storage space, scientists develop new target enrichment technology to overcome these shortages when they only want to focus on the specific regions of interest. This method not only largely reduces cost but also makes the data analysis much easier for researchers.

Currently, two main target enrichment platforms are used in NGS, one is hybridization-based enrichment and the other is amplicon-based enrichment. GenScript uses comprehensive and rich experience molecular biology service and product platform to generate various of solutions in target enrichment for our customers.

Hybridization-based enrichment

• GenFisher Capture Probes Library
• GenFisher Hybridization and Wash Kit

Amplicon-based enrichment

• Custom Multiplex PCR Primers

GenFisher Capture Probes Library

Powered by GenScript’s strong expertise in de novo DNA synthesis and our semiconductor-based electrochemical oligo synthesis technology, we can provide a panel of DNA capture probes library precisely designed for your specific genes of interest.

Service basic information

• 120 nt DNA probes library
• Target region starts from 100 Kb
• Flexible reaction number available

Service specification

• ✔ Consistent and stable quality
• ✔ Design ability and access to probe sequence
• ✔ Self-developed NGS library preparation kit and perfect compatibility
• ✔ Competitive price and TAT at scalable reactions

Case Study

Performance of GenScript’s double-stranded capture probes versus commercially available single-stranded probes

Sequences from a predesigned commercial panel of ~8,000 probes against 127 genes were synthesized by GenScript using an array oligo synthesis platform to create 120 mer, double-stranded DNA (dsDNA) capture probes. For comparison, individually synthesized, single-stranded DNA (ssDNA) probes with the same sequences were obtained from a competing vendor. A DNA library created from a commercial available standard sample was enriched for a 800 kb target region using either capture panel of DNA probes.

Deep, uniform sequence coverage of targeted regions.

Side-by-side comparison of GenScript’s dsDNA capture probes to column-synthesized ssDNA probes (Vendor T) revealed no difference in sequencing coverage depth, indicating comparable quality. An additional panel of dsDNA probes with the same sequences showed similar performance, indicating consistency between synthesized panels.

High reproducibility of capture efficiency.

Comparison of sequencing results between two technical replicates reveals low variation and high reproducibility using GenScript’s dsDNA probes.

GenFisher Hybridization and Wash Kit

GenFisher Hybridization and Wash Kit includes all reagents, beads of capturing custom target regions of a human DNA library, using GenFisher Capture Probes Library and reagents provided in the kit. The captured libraries are ready for subsequent DNA sequencing on Illumina Sequencing Platform.

GenFisher Hybridization and Wash Kit - Easy 3 Steps!

• 

  Hybridization

  This process mixes the DNA library (or libraries) with capture probes of targeted regions.

• 

  Bead Capture and Wash

  This process capture the probes containing the target region.

• 

  Post-Capture PCR

  This process amplifies the capture target enrichment libraries and then purifies them for sequencing.

GenFisher Hybridization and Wash Kit - Order information

The key to excellent target enrichment using DNA hybridization relies on the use of high quality capture probes. Creating optimized target enrichment probes improves the efficiency of targeted NGS and reduces the number of sequencing reads needed to obtain high-confidence data.

Please contact us for pricing and turnaround time by email oligo@genscript.com or phone 1-877-436-7274 (Toll-Free)/ 1-732-885-9188. Our technical account managers will contact you within 24 hours.

Related products

Please contact us for pricing and turnaround time by email oligo@genscript.com or phone 1-877-436-7274 (Toll-Free)/ 1-732-885-9188. Our technical account managers will contact you within 24 hours.

Documents and Protocols

• GenFisher Hybridization and Wash Kit User Guide