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Home » Oligo Synthesis Applications » NGS Target Enrichment

Overview

As whole genome NGS always leads to high cost, long experiment time and large data storage space, scientists develop target enrichment technology to overcome these shortages when they only want to focus on the specific regions of interest. This method not only largely reduces cost but also makes the data analysis much easier for researchers.

Currently, two main target enrichment platforms are used in NGS, one is hybridization-based enrichment and the other is amplicon-based enrichment. GenScript uses comprehensive and rich experience molecular biology service and product platform to generate various of solutions in target enrichment for our customers.

Competitive Advantages

Precise quantification

  • Low batch-to-batch difference to ensure high homogeneity of multiple target fragments obtained

QC for each sequence before pooling

  • MS (deviation≤0.05%) & quantification (deviation≤10%) for each sequence before pooling

Automatic & Precise Pooling

  • Up to ten thousands oligos per pool without losing sequence by high-precision automatic equipment

ISO 13485 certificated

  • High quality & consistency for NGS experiment or NGS panel
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Hybridization-based enrichment

  • Column synthesized 5’-biotinylated ssDNA capture probes for lab developed test scale-up

    Column synthesized ssDNA probe panel is a good choice once you have done small scale test of your target enrichment panel and are ready to scale up to test more populations. The column synthesized ssDNA panel are consist of individually synthesized DNA oligos, purified via RPC, HPLC+, inspected under stringent QC to offer superior purity, consistency and uniformity.

    Types Length Purification method Deliverables
    Capture probes with 5’ biotin 80-150 nt Desalt & Ultrafiltration
    HPLC+
    • Tubes or plates
    • Lyophilized
    • COA document

    Please find more customized QC information in Oligo Quality Control Service>>.

    Column based Custom Capture Probe Panel Performance and specifications: Read More »

    • Highly stable biotin modification

      Stability of biotin modification- storage days
      Stability of biotin modification- frozen times

      Stability of biotin modification with increasing days in storage and number of freeze-thaws.

    • Uniform pooling ability

      TECAN Liquid Handling Platforms

      TECAN Liquid Handling Platforms

      MS (deviation≤0.05%) & quantification (deviation≤10%) for each sequence before pooling

      Up to ten thousands oligos per pool without losing sequence to request concertation by high-precision automatic equipment

    Order:

    For quote, please download the Quick Quotation Requrest Form and send the form to the email address: [email protected]. Our technical account managers will contact you in 24 hours.

Amplicon-based enrichment

  • Custom Multiplex PCR Primers, fully customizable for your convenience

    Similar to column synthesized capture probes, our multiplex PCR primers offers high purity, low contamination primers that delivers in tube or plates for your convenience.

    Length Purification method Deliverables
    15-150 nt Desalt & Ultrafiltration
    HPLC+
    • Tubes or plates
    • Lyophilized
    • COA document

    Please find more customized QC information in Oligo Quality Control Service>>.

    Column based Multiplex PCR Primers Panel Performance and specifications: Read More »

    • Precise quantification and minimal lot-to-lot variation

      Precise quantification and minimal lot-to-lot variation

    • Uniform pooling ability

      TECAN Liquid Handling Platforms

      TECAN Liquid Handling Platforms

      MS (deviation≤0.05%) & quantification (deviation≤10%) for each sequence before pooling

      Up to ten thousands oligos per pool without losing sequence to request concertation by high-precision automatic equipment

    Order:

    For quote, please download the Quick Quotation Requrest Form and send the form to the email address: [email protected]. Our technical account managers will contact you in 24 hours.

NGS Adapter

  • Adapter with cross-contamination as low as 0.01%

    For NGS library construction, whether the sample is directly fragmented or the target region is obtained by hybridization capture, multiplex PCR amplification, etc., it is necessary to add adapter to each sample fragment by Enzyme-linked method or transposase method. GenScript provide adapter with low cross-contamination to avoid mismatch.

    Types Length Purification method Deliverables
    NGS Adapter 15-80 nt HPLC+
    Desalt
    • Tubes or plates
    • Lyophilized
    • COA document

    Please find more customized QC information in Oligo Quality Control Service>>.

    NGS Adapter Performance and specifications: Read More »

    • Low cross-contamination rate

      internal monitoring of cross contamination rates

    • Stable modification

      Stability of biotin modification- storage days
      Stability of biotin modification- frozen times

      Stability of biotin modification with increasing days in storage and number of freeze-thaws.

    Order:

    For quote, please download the Quick Quotation Requrest Form and send the form to the email address: [email protected]. Our technical account managers will contact you in 24 hours.

Case Studies

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