GenScript Target Enrichment Solutions
As whole genome NGS always leads to high cost, long experiment time and large data storage space, scientists develop target enrichment technology to overcome these shortages when they only want to focus on the specific regions of interest. This method not only largely reduces cost but also makes the data analysis much easier for researchers.
Currently, two main target enrichment platforms are used in NGS, one is hybridization-based enrichment and the other is amplicon-based enrichment. GenScript uses comprehensive and rich experience molecular biology service and product platform to generate various of solutions in target enrichment for our customers.
GenFisher Target Enrichment Solution Flyer
Hybridization-based enrichment
Compatible GenTrack, GenNature, Roche KAPA HyperPrep, Illumina TruSeq and Nextera
GenFisher Hybridization and Wash Kit includes all reagents and beads to capturing custom target regions of a human DNA library. The captured libraries are ready for subsequent DNA sequencing on Illumina Sequencing Platform.
GenFisher Hybridization and Wash Kit - Easy 3 Steps!
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Hybridization
This process mixes the DNA library (or libraries) with capture probes of targeted regions.
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Bead Capture and Wash
This process capture the probes containing the target region.
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Post-Capture PCR
This process amplifies the capture target enrichment libraries and then purifies them for sequencing.
GenFisher Hybridization and Wash Kit Performance and specifications: Read More »
Tech document: Read More »
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1 hr hybridization. Compatible with GenTrack, GenNature, Roche KAPA HyperPrep, Illumina TruSeq and Nextera –Recruiting testers
GenFisher 2.0 Fast Hybridization and Wash Kit use improved recipe to reduce standard 16hr hybridization time to 20min, enabling 1 day workflow from library preparation, target enrichment to loading your sequencer.
The kit includes all reagents and beads to capturing custom target regions of a human DNA library. The captured libraries are ready for subsequent DNA sequencing on Illumina Sequencing Platform.
GenFisher 2.0 Fast Hybridization and Wash Kit performance and specifications: Read More »
Fast hybridization, no compromising on quality.
| Hybridization time | On_Target_Base | 30× Coverage | 20% x MeanDepth (%) | Fold_80 |
|---|---|---|---|---|
| 16 hr | 61.63% | 99.85% | 99.26% | 1.30 |
| 1 hr | 56.52% | 99.81% | 98.57% | 1.53 |
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Please contact [email protected]. Our technical account managers will contact you in 24 hours.
Novel chemistry! Enhance capturing efficiency, simplify pre-capture pooling workflow
GenScript Universal Blockers can enhance your hybrid capture efficiency by blocking the adapter region to avoid non specific binding and subsequent pull down by capture probes. We use novel chemistry in the blockers index binding region.
- Regardless of the length of the index
- Pre-capture multiplexing
- IP protected modification for better accommodation of index region
- Stronger binding to the target sequence
- Enable sample multiplexing
- Better blocking efficiency
GenFisher Hybridization and Wash Kit Performance and specifications: Read More »
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Universal blockers increase
target capture efficiency up to 8-plex
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Consistent capture enhancement for
pre-capture pooling for various index length
Tech document: Read More »
- GenTrack Universal Blockers Quick Manual
- GenTrack Universal Blockers SDS 24 rxn
- GenTrack Universal Blockers SDS 96 rxn
- GenTrack Universal Blockers SDS 384 rxn
- GenNature Universal Blockers Quick Manual
- GenNature Universal Blockers SDS 24 rxn
- GenNature Universal Blockers SDS 96 rxn
- GenNature Universal Blockers SDS 384 rxn
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Semiconductor chip synthesized dsDNA probe panel
Powered by GenScript’s strong expertise in de novo DNA synthesis and our semiconductor-based electrochemical oligo synthesis technology, we can provide a panel of DNA capture probes library precisely designed for your specific genes of interest.
- Excellent consistency and Uniformity
- Precisely controlled probe sequence and quantity
- Scalable and fast turnaround time
- NGS QC available
- Cost-effective approach of target enrichment at discovery
GenFisher Custom Capture Probe Panel Performance and specifications: Read More »
Case Study: Read More »
Performance of GenScript’s double-stranded capture probes versus commercially available single-stranded probes
Sequences from a predesigned commercial panel of ~8,000 probes against 127 genes were synthesized by GenScript using an array oligo synthesis platform to create 120 mer, double-stranded DNA (dsDNA) capture probes. For comparison, individually synthesized, single-stranded DNA (ssDNA) probes with the same sequences were obtained from a competing vendor. A DNA library created from a commercial available standard sample was enriched for a 800 kb target region using either capture panel of DNA probes.
Deep, uniform sequence coverage of targeted regions.
Side-by-side comparison of GenScript’s dsDNA capture probes to column-synthesized ssDNA probes (Vendor T) revealed no difference in sequencing coverage depth, indicating comparable quality. An additional panel of dsDNA probes with the same sequences showed similar performance, indicating consistency between synthesized panels.
High reproducibility of capture efficiency.
Comparison of sequencing results between two technical replicates reveals low variation and high reproducibility using GenScript’s dsDNA probes.
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| Catalog_no | Product name | Reaction size |
|---|---|---|
| SC2043 | GenFisher Custom Capture Probes Panel | 24 |
| 96 | ||
| 384 |
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Column synthesized 5’-biotinylated ssDNA capture probes for lab developed test scale-up
Column synthesized ssDNA probe panel is a good choice once you have done small scale test of your target enrichment panel and are ready to scale up to test more populations. The column synthesized ssDNA panel are consist of individually synthesized DNA oligos, purified via RPC, PAGE+ or HPLC+, inspected under stringent QC to offer superior purity, consistency and uniformity.
| Types | Length | Purification method | Deliverables |
|---|---|---|---|
| Capture probes with 5’ biotin | 80-150 nt | RPC PAGE+ HPLC+ |
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Column based Custom Capture Probe Panel Performance and specifications: Read More »
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Highly stable biotin modification
Stability of biotin modification with increasing days in storage and number of freeze-thaws.
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Extremely low error rate
The actual molecular weight (MW) of the probe is 37185 Da, which is consistent with theoretical MW.
Order:
For quote, please download the NGS oligos template and send the form to the email address: [email protected]. Our technical account managers will contact you in 24 hours.
Amplicon-based enrichment
Custom Multiplex PCR Primers, fully customizable for your convenience
Similar to column synthesized capture probes, our multiplex PCR primers offers high purity, low contamination primers that delivers in tube or plates for your convenience.
| Length | Purification method | Deliverables |
|---|---|---|
| 15-150 nt | Desalt+ PAGE+ HPLC+ |
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Column based Custom Capture Probe Panel Performance and specifications: Read More »
Precise quantification and minimal lot-to-lot variation
Order:
For quote, please download the NGS oligos template and send the form to the email address: [email protected]. Our technical account managers will contact you in 24 hours.
