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Home » Oligo Synthesis Applications » NGS Target Enrichment

Overview

As whole genome NGS always leads to high cost, long experiment time and large data storage space, scientists develop target enrichment technology to overcome these shortages when they only want to focus on the specific regions of interest. This method not only largely reduces cost but also makes the data analysis much easier for researchers.

Currently, two main target enrichment platforms are used in NGS, one is hybridization-based enrichment and the other is amplicon-based enrichment. GenScript uses comprehensive and rich experience molecular biology service and product platform to generate various of solutions in target enrichment for our customers.

Hybridization-based enrichment

  • Column synthesized 5’-biotinylated ssDNA capture probes for lab developed test scale-up

    Column synthesized ssDNA probe panel is a good choice once you have done small scale test of your target enrichment panel and are ready to scale up to test more populations. The column synthesized ssDNA panel are consist of individually synthesized DNA oligos, purified via RPC, PAGE+ or HPLC+, inspected under stringent QC to offer superior purity, consistency and uniformity.

    Types Length Purification method Deliverables
    Capture probes with 5’ biotin 80-150 nt RPC
    PAGE+
    HPLC+
    • Tubes or plates
    • Lyophilized
    • COA document

    Column based Custom Capture Probe Panel Performance and specifications: Read More »

    • Highly stable biotin modification

      Stability of biotin modification with increasing days in storage and number of freeze-thaws.

    • Extremely low error rate

      The actual molecular weight (MW) of the probe is 37185 Da, which is consistent with theoretical MW.

    Order:

    For quote, please download the NGS oligos template and send the form to the email address: [email protected]. Our technical account managers will contact you in 24 hours.

Amplicon-based enrichment

  • Custom Multiplex PCR Primers, fully customizable for your convenience

    Similar to column synthesized capture probes, our multiplex PCR primers offers high purity, low contamination primers that delivers in tube or plates for your convenience.

    Length Purification method Deliverables
    15-150 nt Desalt+
    PAGE+
    HPLC+
    • Tubes or plates
    • Lyophilized
    • COA document

    Column based Custom Capture Probe Panel Performance and specifications: Read More »

    Precise quantification and minimal lot-to-lot variation

    Order:

    For quote, please download the NGS oligos template and send the form to the email address: [email protected]. Our technical account managers will contact you in 24 hours.

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