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Webinars » Can CRISPR/Cas9 off-target genomic editing be avoided? Ways to improve target specificity

Can CRISPR/Cas9 off-target genomic editing be avoided? Ways to improve target specificity

Have you tried to generate knock-out or knock-in cell lines using CRISPR/Cas9 in your lab only to find that genes other than your intended target were affected as well? In this webinar, we will review current data on improving the fidelity of CRISPR/Cas9, including better ways to design the sgRNA, and using the double nickase mutant form of Cas9 to avoid off-target effects. We will also discuss best practices in quality control against potential off-target effects and how our GenCRISPR™ service can provide you with a high-quality, genome edited cell line.




Can CRISPR/Cas9 off-target genomic editing be avoided? Ways to improve target specificity
gRNA sequence databases CRISPR gRNA libraries GenCRISPR™ mammalian cell line services
  • Validated gRNA sequences for efficient, specific targeting of WTCas9 or SAM
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  • Target every gene in a single screen
  • Single- or dual- vectors available
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  • KO mammalian cell lines and cell pools
  • Both viral- and non-viral transfection options

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