Are you looking for new methods to design and engineer genes, pathways and even chromosomes? Leslie Mitchell, Ph.D., one of the leading scientists working on the Synthetic Yeast genome project Sc2.0 (www.syntheticyeast.org), will present their ambitious work to build the first synthetic eukaryotic genome. Sc2.0 aims to design, construct, and replace the native 12Mb genome of Saccharomyces cerevisiae with a fully synthetic version. Sc2.0 chromosomes are designed to encode myriad changes. First, to improve genomic stability, destabilizing elements such as transposons are removed and tRNA genes are re-located to a separate 'neochromosome'. Second, synonymously recoded sequences called PCRtags permit encryption and tracking of the synthetic DNA. Finally, to enable downstream genetic flexibility, Sc2.0 encodes an inducible evolution system called SCRaMbLE (Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution) that can generate combinatorial genetic diversity on command. To date, construction has begun on more than half of the 16 synthetic chromosomes by an international team of collaborators. Software and experimental infrastructure developed to facilitate Sc2.0 genome design and construction are applicable to new projects ranging from single gene/pathway design to synthesizing artificial chromosomes.
| Building a synthetic eukaryotic genome: design, construction and application | ||
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| Gene Synthesis | GenBrick™ Synthesis | CRISPR gRNA libraries |
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