Genome editing has always been a challenging area as a means to provide more efficient ways to create a meaningful change in the genome. Unlike the previous gene editing techniques, CRISPR technology can modify DNA with greater precision. This technology has become widely used due to its simplicity and versatility, and the CRISPR technology has been adapted for diverse applications. In this presentation, we will discuss the advantages and disadvantages of CRISPR/Cas9 delivery options and different donor template choices for CRISPR Knock-in. A few case studies to show how to use GenScript’s proprietary semiconductor-based electrochemical oligo synthesis technology to generate CRISPR gRNA libraries, CRISPR KO/KI stable cell line and assay cell line.
In this webinar, you will learn:
- Brief introduction about CRISPR/Cas9 genome editing systems.
- Technologies available for each knock-out and knock-in applications.
- To illustrate the use of CRISPR system, as a powerful tool, for genome editing with case studies.
Dr. Edward Wong is a field application scientist in GenScript. He completed his PhD degree from University of Adelaide. He worked as a Post Doctoral fellow at National Cancer Centre Singapore for 3 years and he had successfully reported the predictive factors for genetic screening of BRCA1 and BRCA2. He also reported the importance of genetic testing for 25 breast cancer-predisposition genes using NGS technology. Both of his works were reported in PlosOne and npj Genomic Medicine.