Protein engineering is a young discipline, of which it modifies the protein sequence in order to develop useful or valuable proteins through improving the substrate specificity, thermostability, binding affinity and so on. Traditionally, both rational designs and directed evolutions are both commonly used approaches in protein engineering. Both of these approaches possess its advantages and disadvantages.
Here, GenScript presented a third option, namely semi-rational design, to modify the proteins. With the new advanced technology, GenScript offers a powerful and high-throughput tool called precision mutant library.
In this webinar, you will learn:
- The importance of key features of mutant libraries and how they affect downstream applications.
- Advantages of using semiconductor-based oligo synthesis in the construction of saturation or combinatorial mutagenesis libraries.
- Case studies on how this technology can create a more diverse and precise mutant library to screen for optimized protein structure and function through precise control on synthesized variant but also to expedite downstream screening.
Precision Mutant Library helps in Protein Engineering
Dr. Edward Wong is a field application scientist in GenScript. He completed his PhD degree from University of Adelaide. He worked as a Post Doctoral fellow at National Cancer Centre Singapore for 3 years and he had successfully reported the predictive factors for genetic screening of BRCA1 and BRCA2. He also reported the importance of genetic testing for 25 breast cancer-predisposition genes using NGS technology. Both of his works were reported in PlosOne and npj Genomic Medicine.