The T cell epitope is a small peptide sequence, presented by MHC receptors on antigen presenting cells and other nucleated cells, that stimulates immune system activity. For many diseases, such as viral diseases and cancer, T cells cannot adequately recognize virus or cancer-cell epitopes. Re-engineering the T cell receptor enables the T cell to recognize and bind these epitopes more efficiently. In doing so, we can stimulate the body's own immune system to target and fight diseases that normally are difficult to treat.

In most cases, immunotherapy first requires identification of the most immuno-stimulatory epitopes on viruses or cancer cells. The T cell epitopes are short peptide sequences, ranging from 8-11 amino acids in length on MHC I complexes and 15-24 amino acids on MHC II complexes. To identify these small peptide sequences within the larger epitope, peptide libraries are ideal, since they span hundreds of peptide combinations simultaneously. The T cell truncated peptide library is unique, in that it is composed of sequentially truncated peptide combinations, thereby ensuring that the entire T cell epitope is spanned. These truncated peptide fragments, commonly ranging from 11- to 8-mers, are pooled. Once a positive pool has been identified, the pool can be deconvoluted to identify the T cell epitope sequence.

To use GenScript's T cell truncated peptide library tool, first enter the protein sequence of interest. Then, select the length of the longest peptide sequence in the pool (for MHC I, this will be 11, while for MHC II the longest length can be up to 24 AA). The tool will generate pools of 11-mer, 10-mer-, 9-mer, and 8-mer peptides that span the entire sequence length (or for other peptide sequences, the lengths will be n-mer, (n-1)-mer, (n-2)-mer, and (n-3)-mer).

Applications of T-Cell Truncated Library

  • Allow to test all possible T-cell epitopes across a protein of interest
Overlapping Peptide
Alanine Scanning
Truncation Library
Positional Scanning
Random Library
Scrambled Library
T Cell Truncated Library


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