A positional scanning library is an important tool for peptide sequence optimization. Amino acids of interest at a given position(s), are substituted with all other natural amino acids, one at a time. This allows for amino acids that enhance peptide activity to be identified.
In particular, positional scanning libraries have been used to identify T-cell epitopes from complex mixtures of proteins. In addition, this type of library can also be used to locate substrates with interdependent subsites with only minimum synthesis and screening.
- Pinilla C, Appel JR, Campbell GD, Buencamino J, Benkirane N, Muller S, and Greenspan NS. All-D peptides recognized by an anti-carbohydrate antibody identified from a positional scanning library. J. Mol. Biol. Nov 1998 13; 283(5): 1013-25.
- Lee CH, Lee MS, Kim SJ, Je YT, Ryu SH, and Lee T. Identification of novel synthetic peptide showing angiogenic activity in human endothelial cells. Peptides. Oct 2008 17.
- Chaparro RJ, Burton AR, Serreze DV, Vignali DA, and DiLorenzo TP. Rapid identification of MHC class I-restricted antigens relevant to autoimmune diabetes using retrogenic T cells. J. Immunol. Methods. Jun 2008 1; 335(1-2): 106-15.
- Nino-Vasquez JJ, Allicotti G, Borras E, Wilson DB, Valmori D, Simon R, Martin R, and Pinilla C. A powerful combination: the use of positional scanning libraries and biometrical analysis to identify cross-reactive T cell epitopes. Mol. Immunol. Feb 2004; 40(14-15): 1063-74.
- Houghten RA. The broad utility of soluble peptide libraries for drug discovery. Gene. Dec 1993 27; 137(1): 7-11.