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Positional Scanning Library

A positional scanning library is an important tool for peptide sequence optimization. Amino acids of interest at a given position(s), are substituted with all other natural amino acids, one at a time. This allows for amino acids that enhance peptide activity to be identified.

In particular, positional scanning libraries have been used to identify T-cell epitopes from complex mixtures of proteins. In addition, this type of library can also be used to locate substrates with interdependent subsites with only minimum synthesis and screening.

Overlapping Peptide
 
Alanine Scanning
     
Truncation Library
 
Positional Scanning
     
Random Library
 
Scrambled Library
     
T Cell Truncated Library
   

References

 
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