Truncation Library

A truncation library allows researchers to determine the minimum length required for epitope activity. The library is generated through a systematic truncation of the peptide's sequence from each terminus. After the key residues have been identified via Alanine Scanning Library studies, truncation libraries can be used to determine the role of the sequences surrounding key residues.

In many cases, truncation library screening allows for the identification of peptides with enhanced proteolytic stability. It can serve as a tool to investigate the extent to which peptide drugs undergo metabolic degradation, which is a major consideration in bringing drugs to the market.

Applications of Truncation Library

Overlapping Peptide   Alanine Scanning       Truncation Library   Positional Scanning       Random Library   Scrambled Library       T Cell Truncated Library

References

• Svenson J, Stensen W, Brandsdal BO, Haug BE, Monrad J, and Svendsen JS. Antimicrobial peptides with stability toward tryptic degradation. Biochemistry. Mar 2008 25; 47(12): 3777-88.
• Bolger GB, Baillie GS, Li X, Lynch MJ, Herzyk P, Mohamed A, Mitchell LH, McCahill A, Hundsrucker C, Klussmann E, Adams DR, and Houslay MD. Scanning peptide array analyses identify overlapping binding sites for the signalling scaffold proteins, beta-arrestin and RACK1, in cAMP-specific phosphodiesterase PDE4D5. Biochem. J. Aug 2006; 15; 398(1): 23-36.
• Ostermeier M, Nixon AE, Shim JH, and Benkovic SJ. Combinatorial protein engineering by incremental truncation. Proc. Natl. Acad. Sci. U S A. Mar 1999 30; 96(7): 3562-7.
• de Figueiredo P, Roberts RL, and Nester EW. DARTs: A DNA-based in vitro polypeptide display technology. Proteomics. Oct 2004; 4(10): 3128-40.
• Horswill AR, Naumann TA, and Benkovic SJ. Using incremental truncation to create libraries of hybrid enzymes. Methods Enzymol. 2004; 388: 50-60.