Alanine scanning libraries are designed to identify the specific amino acid residues responsible for the peptide's function, stability, and conformation. Alanine, the smallest chiral amino acid, is sequentially substituted for each non-alanine residue one at a time. Subsequently, corresponding change in epitope activity can be measured. Substitution of key amino acid residue(s) with alanine causes changes in epitope binding activity. This library enables quick determination of each individual amino acid's contribution to the peptide's functionality.