Alanine scanning libraries are designed to identify the specific amino acid residues responsible for the peptide's function, stability, and conformation. Alanine, the smallest chiral amino acid, is sequentially substituted for each non-alanine residue one at a time. Subsequently, corresponding change in epitope activity can be measured. Substitution of key amino acid residue(s) with alanine causes changes in epitope binding activity. This library enables quick determination of each individual amino acid's contribution to the peptide's functionality.

Applications of Alanine Scanning Library

Identify key residues in epitopes

Evaluation function property of enzymes

Study binding sites of proteins

Overlapping Peptide
overlapping library
 
Alanine Scanning
alanine scanning library
     
Truncation Library
truncation library
 
Positional Scanning
positional scanning library
     
Random Library
random library
 
Scrambled Library
scrambled library
     
T Cell Truncated Library
T tell truncated tibrary
   

Resource

References

  • Weiss GA, Watanabe CK, Zhong A, Goddard A, and Sidhu SS. Rapid mapping of protein functional epitopes by combinatorial alanine scanning. Proc. Natl. Acad. Sci. U S A. Aug 2000 1; 97(16): 8950-4.
  • Richardson PL. The determination and use of optimized protease substrates in drug discovery and development. Curr. Pharm. Des. 2002; 8(28): 2559-81.
  • Morrison KL, and Weiss GA. Combinatorial alanine-scanning. Curr. Opin. Chem. Biol. Jun 2001; 5(3): 302-7.
  • Levine KB, Hamill S, Cloherty EK, and Carruthers A. Alanine scanning mutagenesis of the human erythrocyte glucose transporter putative ATP binding domain. Blood Cells Mol. Dis. Jan-Feb 2001; 27(1): 139-42.
  • Sidhu SS, and Kossiakoff AA. Exploring and designing protein function with restricted diversity. Curr. Opin. Chem. Biol. Jun 2007; 11(3): 347-54.

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