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Western blot test: 0.1-1 μg/ml
IHC, ICC, FACs and IP test: 1-5 μg/ml
Elisa test: 0.05-0.2 ug/ml
Please read and carefully follow the manual instructions on how to preserve each antibody product.
Typically, store antibodies at 2-8C for short term storage and store at -20C for long term storage. Aliquot and store before use to avoid repeated freeze-thaw cycles.
The following antibody products need to be stored in the dark.
To ensure antibody quality between different batches, our QA tests and compares sensitivity and specificity between batches
Both Polyclonal and Monoclonal antibodies have their own advantages which make them useful for different applications.
Large quantities of polyclonal antibody are relatively quick and inexpensive to produce compared to monoclonal antibodies. They are non-specific in that they are capable of recognizing multiple epitopes on any one antigen.
The brightness of fluorophore and the antibody's sensitivity are two factors that influence detection sensitivity. THE™ Elite antibody series have the best sensitivity on the market. The brighter the fluorophore is, the better the overall detection.
GenScript's iFluor antibody series are newly developed fluorescent compounds conjugated to THE™ Elite antibody series. Compared with traditional fluorescent dyes, the iFluor series are brighter and have better stability.
GenScript has four types of fluorophore conjugated His-tag antibodies for FACS analysis, including A01620 (FITC), A01800 (iFluor488), A01801 (iFluor555) and A01802 (iFluor 647).
The iFluor series are recommended for FACS analysis because they have a higher sensitivity than FITC conjugated antibody. The following is the wavelength range of GenScript's iFluor antibodies:
According to our FACS analysis results, the sensitivity of A01802 (THE™ His Tag Antibody [iFluor 647], mAb, Mouse) is slightly higher than the A01800 (THE™ His Tag Antibody [iFluor 488], mAb, Mouse). If the His Tag number is low, A01802 is recommended.
For A00186, a starting point of 1 µg/ ml is recommended.
The catalog No. is A00186 for the anti-His mAb in the L00436 ELISA Kit. The recommended concentration for WB is 0.1-0.2 µg/ml.
Catalog No. A00174 is a rabbit anti-His tag antibody. It has been tested for internal His-tag recognition, but not for Flow Cytometry.
For Flow Cytometry, the following antibodies are recommended. All of them can recognize His-tag fused on the internal site of a protein.
We have not yet tested PEG5. We tested PEG12 with positive results. One of our clients tested PEG2 and observed good results.
In most cases it should be OK to conjugate an antibody (IgG/IgM/IgY) to agarose resin. Our PEG antibody could be used for this conjugation.
As mentioned in the datasheet, THE™ PEG Antibody, mAb, mouse should be stored in its lyophilized form until use. It remains stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze-thaw cycles.
Reconstitute the lyophilized product with deionized water (or equivalent) to make an antibody concentration of 0.5 mg/ml. For long-term storage, it is recommended to add glycerol to a final concentration of 50% (v/v) in the reconstituted antibody solution.
When using [HRP] tagged primary antibodies there are some variables that may affect its binding with a secondary antibody.
Is Catalog No. A00166 (Goat Anti-Human IgG Antibody (H&L) [HRP], pAb) specific for human IgG. Does it cross-react with non-human primates or rodents?
Catalog No. A00166 (Goat Anti-Human IgG Antibody (H&L) [HRP], pAb) recognizes human IgG, but it has not yet been tested for cross-reactivity with other species.
Does Catalog No. A00160 (Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb) cross react with rabbit?
Catalog. No A00160 (Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb) has not yet been tested for cross-reactivity with rabbit.
The immunogen of A01622 is human GAPDH protein from erythrocytes. A01622 detects endogenous levels of human, pig, cow, goat, and fish GAPDH. This product has not been tested for other species.
A00191 (GAPDH Antibody, pAb, Goat) specifically reacts with GAPDH from cell and tissue lysates of human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli.
The target protein tested is 57KDa because it is the recombinant protein, not the naturally occurring protein with the molecular weight of 18KDa.
From the COA file, you can see that the fusion partners for this protein are calbindin, Trx tag and Strip-Ⅱtag.
According to multiple sequence alignment results, there is only one sequence in the recombinant protein with the peptide antigen for immunization. Based on these results there should be no cross-reactivity between the fusion partner and PIN1.
PIN1 is fused to its partner at the N terminal.
Please contact us if you need immediate assistance.
Please contact us (phone: 1-877-436-7274 or email: firstname.lastname@example.org),if you need immediate assistance.