Green PCR Reagents
GenScript's advanced high-stability technology has led to the invention of the new generation of PCR reagents—Green PCR Reagents. The Green series of reagents, from the proprietary Taq, dNTP, to PCR mixtures, are stable for one month if stored at room temperature, or for over six months if stored at 4°C. The high-stability feature comes with increased half-life of active enzymes at high temperatures, which results in more amplification cycles and higher yield. With no freeze-thaw cycles, green PCR reagents provide you an instant access to high-throughput PCR automation. In addition, GenScript's Green PCR Reagents can help preserve energy associated with storage and shipping.
Key Features
- S table at room temperature
- Enhanced yields with longer enzyme half-life
- Stop refrigerating and save energy
Green Taq DNA Polymerase
Green Taq DNA Polymerase has terminal transferase activity that results in the addition of single nucleotide (adenosine) at 3'-end of the extension product. This enzyme also has a longer enzyme half-life and therefore increases the PCR yields when amplifying long DNA fragments.
Ordering Information
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
E00043 |
Green Taq DNA Polymerase - 1000 U (1000.0U/vial) |
$66.00 |
High-Stability PCR Kit
GenScript's High-Stability PCR Kit contains Green Taq DNA Polymerase, 10X PCR buffer, and 10 mM dNTP, all specially formulated for higher stability using our proprietary technology. The stabilizers in the storage buffer also increase the stability of Taq DNA Polymerase at higher temperatures (72°C), increasing the PCR yield of long DNA amplification. This Kit makes the PCR step quick and easy by eliminating any need for thawing. This kit can be used in high-throughput assays in which all the PCR reagents need to be ready and stable for PCR at any time.
Ordering Information
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
L00342 |
High-Stability PCR Kit - 1 kit |
$68.00 |
Specification of High-Stability PCR Kit
| Components | Volume | Documents |
|---|---|---|
| Green Taq DNA polymerase | 100 μl |  |
| 10X reaction buffer | 1.5 ml | |
| Stabilized dNTP | 300 μl | |
| Protocol | 1 |
- The Green Taq DNA polymerase provides high PCR yield and specificity when amplifying long DNA fragments.
- The Green Taq DNA polymerase can be stored at 37°C for 14 days without activity loss.
Figure 1. An 8-kb fragment from λDNA was amplified by using both Taq DNA polymerase and Green Taq DNA polymerase. For long DNA fragment amplification, where longer elongation time is needed (In this case, the elongation time was eight minutes.), the Green Taq DNA polymerase has a longer half-life and therefore increases the PCR DNA yields significantly.
Figure 2. A 2-kb 23S rRNA fragment from from E. coli genomic DNA was amplified using Green Taq DNA polymerase stored at -20°C and 37°C, respectively.
Citations
- Scott C., et al. Dioxygenases catalyze O-demethylation and O, O-demethylenation with widespread roles in benzylisoquinoline alkaloid metabolism in opium poppy. J Biol Chem. 2013Aug;
- Wang X., et al. Salicylic Acid Regulates Plasmodesmata Closure during Innate Immune Responses in Arabidopsis. Plant Cell. 2013 Jun;25(6):2315-29.
- Ruth B. Phillips., et al. Characterization of the OmyY1 Region on the Rainbow Trout Y Chromosome. Int J Genomics. 2013 Mar;261730.
- FrÍas M., et al. The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif in the protein surface. Mol Plant Pathol. 2013 Qct;12097.
- Y Acanda., et al. EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine. Plant Cell Rep. 2013 Dec.
- Firlej A., et al. A Multi-Approach Study to Delineate Interactions Between Carabid Beetles and Soybean Aphids. Environ Entomol. 2013 Feb;42(1):89-96.
- Zhou P., et al. IGF-I Signaling Is Essential for FSH Stimulation of AKT and Steroidogenic Genes in Granulosa Cells. Mol Endocrinol. 2013 Mar;27(3):511-523.
- Wang X., et al. Silencing of the Host Factor eIF (iso) 4E Gene Confers Plum Pox Virus Resistance in Plum. PLoS One. 2013 Jan;8(1):e50627.
- Chiorazzi M., et al. Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma. Proc Natl Acad Sci U S A. 2013 Mar;110(10):3943-3948.
- Doyle VP., et al. Habitat and Host Indicate Lineage Identity in Colletotrichum gloeosporioides sl from Wild and Agricultural Landscapes in North America. PLoS One. 2013 May;6(5):e62394.
- Jun TH., et al. Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324. Heredity (Edinb). 2013 Jul;111(1):16-22.
- Carol D. von Dohlen., et al. Diversity of proteobacterial endosymbionts in hemlock woolly adelgid (Adelges tsugae)(Hemiptera: Adelgidae) from its native and introduced range. Environ Microbiol. 2013 Jul;15(7):2043-62.
- Gandhirajan RK., et al. Blockade of NOX2 and STIM1 signaling limits lipopolysaccharide-induced vascular inflammation. J Clin Invest. 2013 Feb;123(2):887-902.
- Zhou JB., et al. Identification of a novel gene fusion RNF213â€'SLC26A11 in chronic myeloid leukemia by RNA-Seq. Mol Med Rep. 2013 Feb;7(2):591-7.
- Jessica Hoppstdter., et al.Glucocorticoid-induced leucine zipper is downregulated in human alveolar macrophages upon Toll-like receptor activation. Eur J Immunol. 2012 May;42(5):1282-93.
- Sheng H., et al. Mapping QTL for resistance to eyespot of wheat in Aegilops longissima. Theor Appl Genet. 2012 Jul;125(2):355-366.
Ordering Information
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
E00007-50000 |
Taq DNA Polymerase - 50000 U (1000.0U/vial) |
$2970.00 |
|
E00007-1000 |
Taq DNA Polymerase - 1000 U (1000.0U/vial) |
$66.00 |
|
E00008 |
Taq DNA Polymerase without Mg2+ - 1000 U (1000.0U/vial) |
$66.00 |
|
A01849-250 |
Hot Start Taq Antibody - 250 U (5U/μl) |
$126.00 |
|
A01849-1000 |
Hot Start Taq Antibody - 1000 U (5U/μl) |
$399.00 |
|
C01689 |
Stabilized dNTP Mix, 10 mM each - 0.5 ml |
$34.00 |
|
E00019 |
2X Taq Master Mix - 100 Reactions (2.5 mL) |
$48.00 |
|
D0056 |
dNTP mixture, 10 mM each - 0.5 ml |
$29.00 |
|
E00101 |
Taq DNA Polymerase (1000 U) with 10 mM dNTP Mix (0.5 ml) - 1 PK |
$86.00 |
|
C01577-50 |
dATP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01577-250 |
dATP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01577-10 |
dATP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01577-1 |
dATP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01578-50 |
dGTP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01578-250 |
dGTP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01578-10 |
dGTP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01578-1 |
dGTP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01579-50 |
dCTP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01579-250 |
dCTP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01579-10 |
dCTP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01579-1 |
dCTP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01580-50 |
dTTP (100 mM) ≥99% HPLC - 50 ml (50ml/bottle) |
$1840.00 |
|
C01580-250 |
dTTP (100 mM) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$8625.00 |
|
C01580-10 |
dTTP (100 mM) ≥99% HPLC - 10 ml (10.0ml/vial) |
$414.00 |
|
C01580-1 |
dTTP (100 mM) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
|
C01582-50 |
dNTP (10 mM each) ≥99% HPLC - 50 ml (50ml/bottle) |
$828.00 |
|
C01582-250 |
dNTP (10 mM each) ≥99% HPLC - 250 ml (250.0ml/bottle) |
$2875.00 |
|
C01582-10 |
dNTP (10 mM each) ≥99% HPLC - 10 ml (10.0ml/vial) |
$178.00 |
|
C01582-1 |
dNTP (10 mM each) ≥99% HPLC - 1 ml(1.0ml/vial) |
$52.00 |
