Specification of High-Stability PCR Kit
|Green Taq DNA polymerase||100 μl||DNA Polymerase PDF|
|10X reaction buffer||1.5 ml|
|Stabilized dNTP||300 μl|
- The Green Taq DNA polymerase provides high PCR yield and specificity when amplifying
long DNA fragments.
- The Green Taq DNA polymerase can be stored at 37°C for 14 days without activity
Figure 1. An 8-kb fragment from λDNA was amplified by using both Taq DNA polymeraseand Green Taq DNA polymerase. For long DNA fragment amplification, where longer elongation time is needed (In this case, the elongation time was eight minutes.), the Green Taq DNA polymerase has a longer half-life and therefore increases the PCR DNA yields significantly.
Figure 2. A 2-kb 23S rRNA fragment from from E. coli genomic DNA was amplified using GreenTaq DNA polymerase stored at -20°C and 37°C, respectively.