Storage Condition | Stable at -20°C for one year. Note: The enzyme can be stable at room temperature or even 37°C for seven days without any loss of activity. |
---|---|
Concentration | 5 U/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol. |
Recombinant (Yes/No) | Yes |
Product Source | E. coli cells |
Definition of Activity Unit | One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. |
10 X Reaction Buffer (with Mg2+) |
500 mM KCl, 100 mM Tris HCl (pH9.0 at 25°C), 15 mM MgCl2, 1%
Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs. Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity. |
Hazardous | No |
5' - 3' Exonuclease | Yes |
3' - 5' Exonuclease | No |
RNases contamination | No |
Error Rate | NA |
PCR performance, activity, nuclease

Lane | Taq | Unit |
---|---|---|
1 | Leading Brand A | 0.1 |
2 | 0.25 | |
3 | 0.5 | |
4 | Leading Brand B | 0.1 |
5 | 0.25 | |
6 | 0.5 | |
7 | GenScript | 0.1 |
8 | 0.25 | |
9 | 0.5 |