Reasonable PCR Enzyme

Applications

Products

Ordering Information

*These products are also aviaiable in larger package sizes or customized requests, please quote

Examples

• Examples

  Storage Condition	Stable at -20°C for one year. Note: The enzyme can be stable at room temperature or even 37°C for seven days without any loss of activity.
  Concentration	5 U/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
  Recombinant (Yes/No)	Yes
  Product Source	E. coli cells
  Definition of Activity Unit	One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
  10 X Reaction Buffer (with Mg2+)	500 mM KCl, 100 mM Tris HCl (pH9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs. Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
  Hazardous	No
  5' - 3' Exonuclease	Yes
  3' - 5' Exonuclease	No
  RNases contamination	No
  Error Rate	NA

  PCR performance, activity, nuclease

  

  Lane	Taq	Unit
  1	Leading Brand A	0.1
  2		0.25
  3		0.5
  4	Leading Brand B	0.1
  5		0.25
  6		0.5
  7	GenScript	0.1
  8		0.25
  9		0.5

Product Resources

• Product Resources

  Protocol »

  • Guideline for PCR Amplification

  MSDS »

  • Taq DNA Polymerase
  • Taq DNA Polymerase without Mg²⁺
  • Taq DNA Polymerase, concentrated
  • 2X Taq Master Mix (PCR Master Mix)

Related Products

• Related Products

  Following PCR related products are also available in larger package sizes.

  Cat. No.	Product Name	Quantity	Price	Order
  A01849	Hot Start Taq Antibody		$126.00 250 U (5U/μl) 1000 U (5U/μl)
  C01577	dATP (100 mM) ≥99% HPLC		$52.00 1 ml(1.0ml/vial) 10 ml (10.0ml/vial) 50 ml (50ml/bottle) 250 ml (250.0ml/bottle)
  C01578	dGTP (100 mM) ≥99% HPLC		$52.00 1 ml(1.0ml/vial) 10 ml (10.0ml/vial) 50 ml (50ml/bottle) 250 ml (250.0ml/bottle)
  C01579	dCTP (100 mM) ≥99% HPLC		$52.00 1 ml(1.0ml/vial) 10 ml (10.0ml/vial) 50 ml (50ml/bottle) 250 ml (250.0ml/bottle)
  C01580	dTTP (100 mM) ≥99% HPLC		$52.00 1 ml(1.0ml/vial) 10 ml (10.0ml/vial) 50 ml (50ml/bottle) 250 ml (250.0ml/bottle)
  C01582	dNTP (10 mM each) ≥99% HPLC		$52.00 1 ml(1.0ml/vial) 10 ml (10.0ml/vial) 50 ml (50ml/bottle) 250 ml (250.0ml/bottle)
  C01689	Stabilized dNTP Mix, 10 mM each		$35.00 0.5 ml
  C01717	dUTP (100 mM) ≥99% HPLC		$76.00 1 ml(1.0ml/vial) 10 ml(10.0ml/vial) 50 ml (50ml/bottle) 250 ml(250.0ml/bottle)
  D0056	dNTP mixture, 10 mM each		$34.00 0.5 ml
  E00043	Green Taq DNA Polymerase		$66.00 1000 U (1000.0U/vial)
  L00342	High-Stability PCR Kit		$68.00 1kit Kit