|Storage Condition||Stable at -20°C for one year.
Note: The enzyme can be stable at room temperature or even 37°C for seven days without any loss of activity.
|Concentration||5 U/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.|
|Product Source||E. coli cells|
|Definition of Activity Unit||One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.|
|10 X Reaction Buffer
|500 mM KCl, 100 mM Tris HCl (pH9.0 at 25°C), 15 mM MgCl2, 1%
Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs.
Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
|5' - 3' Exonuclease||Yes|
|3' - 5' Exonuclease||No|
PCR performance, activity, nuclease
|1||Leading Brand A||0.1|
|4||Leading Brand B||0.1|