Overview

Custom synthesized peptides from GenScript are manufactured under strict high-level quality control processes. Our ISO 9001 certified, Total Quality Management (TQM) platform ensures that each custom peptide is triple checked for quality via both mass spectrometry (MS) and high performance liquid chromatography (HPLC) analyses after each step during peptide purification and quality control (QC) procedures. As the final step in our TQM platform, we perform additional quality assurance (QA) procedures for every custom peptide to further guarantee the delivery of high-quality peptides.

TQM Platform for Peptide Services

TQM Platform for Peptide Services

AccuPepQC+ webpage
For additional customized quality control services that can ensure peptide reliability and reproducibility view our AccuPepQC+ webpage.

ArgonShield™ packing service webpage

View our ArgonShield™ packing service webpage to see how GenScript maintains the integrity of your custom peptides from our lab to yours.

Understanding Your Peptide QC Report

Your peptide QC report consists of MS and HPLC analyses. The guide below will help you understand each analytical report, and how to tell if a quality analysis of your peptide was performed.


  • HPLC report

  • MS report

HPLC report

Even after multiple rounds of purification, small amounts of impurities may still exist in the final peptide product. Therefore, GenScript uses reversed-phase high performance liquid chromatography (RP-HPLC) to analyze the purity of each custom peptide.


What is RP-HPLC?

RP-HPLC is a widely used analytical tool, in which the components of a complex mixture can be efficiently separated. The analyte mixture is usually dissolved in water, which is sometimes also mixed with an organic solvent or an acid to assist dissolution prior to RP-HPLC analysis. The analyte is carried by a mobile phase consisting of water and an organic modifier, which is pumped through a column packed with a stationary phase which usually is a number of small diameter particles consisting of carbon chains of a specified length on the surface. As the mixture is pumped through the column, the analytes (in this case your custom peptide and small impurities) adsorb to the hydrophobic surface of the stationary phase. As the percentage of organic modifier in the mobile phase is gradually increased, the analytes desorb into the mobile phase. Desorption of a specific analyte is based on its intrinsic properties, thus each analyte will remain in the column for a specific amount of time, called the retention time. Since peptide bonds maximally absorb UV light at the wavelength of 220 nm, a UV spectrometer is commonly used in RP-HPLC to detect a peptide as it elutes from the column. The detection signal is converted to a visual graph called a chromatogram, which is the plot of UV absorbance vs. elution time.

High resolution of eluted peptides is mainly based on the selection of columns and the organic modifier, especially its elution gradient. The height of a theoretical plate of each column used in the manufacture is regularly checked, and different gradients are established to separate varying peptides. Both processes are aimed at maintaining high resolution of every custom peptide. GenScript takes special care in developing RP-HPLC protocols to ensure the most effective separation and the accurate purity determination of your custom peptides.

Reading Your Custom Peptide HPLC report

Custom Peptide HPLC report
  1. HPLC mobile phase and its elution gradient: The peptide separation is highly sensitive to the selection of organic modifier and its elution gradient.
  2. Absorption wavelength: The wavelength of 220 nm is chosen for UV detection since peptide bonds (amide bonds) maximally absorb UV at this wavelength.
  3. Column type and dimensions: The selection of the column plays an important role in RP-HPLC analysis. A C18 column is usually used to separate peptides.
  4. Dead time: Indicates the amount of time required for an unretained solute such as the organic solvent or the acid from the column. Thus, the organic solvent nor the acid contributes to the area of the target peptide peak.
  5. Major component elution peak (the target peptide): Shows the retention time of the target peptide as well as the impurities. The integrated peak area is used for the purity calculation. The baseline should be smooth, indicating that the system is stable. The vertical lines separate the partially overlaid peaks and denote the peak area of the target peptide.
  6. Peak table: All peaks shown in the chromatogram are listed according to their retention time. The Area % of the target peptide is used to calculate the peptide HPLC purity.

Quotations and Ordering

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