For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.

Ultrasensitive Detection of SARS-CoV-2 Spike Proteins Using the Thio-NAD Cycling Reaction: A Preliminary Study before Clinical Trials

Microorganisms. 2021-10; 
Yuta Kyosei, Mayuri Namba, Daiki Makioka, Ayumi Kokubun, Satoshi Watabe, Teruki Yoshimura, Tadahiro Sasaki, Tatsuo Shioda, Etsuro Ito
Products/Services Used Details Operation
Mammalian Expression … The recombinant antigen for SARS-CoV-2 spike S1 protein was obtained from GenScript (human cell source, 78.3 kDa, Cat #Z03501). Alkaline phosphatase (ALP) and nicotinamide adenine dinucleotide (NADH) were purchased from Roche. The thio-NAD was obtained from … Get A Quote


To help control the global pandemic of coronavirus disease 2019 (COVID-19), we developed a diagnostic method targeting the spike protein of the virus that causes the infection, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We applied an ultrasensitive method by combining a sandwich enzyme-linked immunosorbent assay (ELISA) and the thio-nicotinamide adenine dinucleotide (thio-NAD) cycling reaction to quantify spike S1 proteins. The limit of detection (LOD) was 2.62 × 10 moles/assay for recombinant S1 proteins and 2.6 × 10 RNA copies/assay for ultraviolet B-inactivated viruses. We have already shown that the ultrasensitive ELISA for nucleocapsid proteins can detect ultraviolet B-inactivated viru... More


COVID-19, SARS-CoV-2, antigen test, spike protein, thio-NAD cycling, ultrasensitive ELISA