We have four types of fluorophore conjugated His-tag antibodies for FACS analysis,
including A01620(FITC), A01800(iFluor488), A01801(iFluor555) and A01802(iFluor 647).
The iFluor series are recommended for FACS analysis because they have a higher
sensitivity than FITC conjugated antibody. The following is the wavelength range of GenScript's
iFluor antibodies:
Fluorophore Characterization
Cat. No. |
Conjugate |
Ex (nm) |
Em (nm) |
A01800
|
iFluor 488
|
491
|
514
|
A01801
|
iFluor 555
|
559
|
569
|
A01802
|
iFluor 647
|
654
|
674
|
According to our FACS analysis results, the sensitivity of A01802 (THE™ His Tag
Antibody [iFluor 647], mAb, Mouse) is slightly higher than the A01800 (THE™ His Tag Antibody
[iFluor 488], mAb, Mouse). If the His Tag number is low, A01802 is recommended.