Overview

Protein A ELISA Kit

Cat. No. Product Name Quantity Price Order
L00430
Protein A ELISA Kit
$587.00 1 kit
 

Protein A affinity chromatography is widely used for antibody purification. However, the leakage of Protein A, though covalently conjugated to chromatography supports, posts a big problem for antibody based pharmaceutical industry. Moreover, it also probably causes false results in immunological assays if antibody product is contaminated with Protein A.

GenScript Protein A ELISA Kit is developed for accurate quantification of the leakage of native Protein A, recombinant Protein A variants and MabSelect SuRe in the presence or absence of IgG antibodies. The kit offers users in the pharmaceutical industry a sensitive assay to measure mini amounts of Protein A in therapeutic antibody products. It also facilitates Protein A resin manufacturers in monitoring leaching characteristics of the resins under specific conditions.

Key Features

Accurate detection of 20 pg/ml Protein A and 50 pg/ml MabSelect SuRe in antibody preps

Accurate detection of 20 pg/ml Protein A and 50 pg/ml MabSelect SuRe in antibody preps

Broad-range recognition of native Protein A, recombinant Protein A variants and MabSelect SuRe

Broad-range recognition of native Protein A, recombinant Protein A variants and MabSelect SuRe

Low level deviation - Intra-assay <5%, Inter-assay <10%

Low level deviation - Intra-assay <5%, Inter-assay <10%

High accuracy/recovery within 85 -115%

High accuracy/recovery within 85 -115%

Ready-to-use ELISA plate with fast & simple samples preparation

Time-saving protocol - complete assay in less than 2 hours

Ready-to-use ELISA plate with fast & simple samples preparation

Ready-to-use ELISA plate with fast & simple samples preparation

Exceptional lot-to-lot consistency ensured by high-quality monoclonal antibodies

Exceptional lot-to-lot consistency ensured by high-quality monoclonal antibodies

Wide applications for the quantification of Protein A contaminant and leaching

Wide applications for the quantification of Protein A contaminant and leaching

Specifications

Components
Quantity
96-well plate coated with anti-Protein A mAb
1 plate (8 wells x 12 strips)
Biotin conjugated anti-Protein A mAb
12 ml
Streptavidin-HRP
12 ml
Protein A Standard (10 μg/ml)
1 ml
MabSelect SuRe Protein A Standard (10 μg/ml)
1 ml
20 × Wash Solution
40 ml
One-Solution Microwell TMB Substrate
12 ml
Stop Solution
6 ml
All components should be stored at 2-8°C.

Examples

  • Typical Assay Data
    Conc. of Protein A (ng/ml)
    OD 450
    Duplicate 1
    Duplicate 2
    Average
    Adjusted Average
    3.20
    1.711
    1.829
    1.770
    1.717
    1.60
    0.849
    0.871
    0.860
    0.807
    0.80
    0.461
    0.468
    0.465
    0.412
    0.40
    0.247
    0.257
    0.252
    0.199
    0.20
    0.163
    0.164
    0.164
    0.111
    0.10
    0.141
    0.149
    0.145
    0.092
    0.00
    0.077
    0.076
    0.077
    0.024
    Blank
    0.052
    0.054
    0.053
    Protein A ELISA Kit

    Figure 1. Standard curve generated using GenScript Protein A ELISA Kit (L00430).

  • Precision
    intra-assay
    inter-assay
    # of tests
    Meanng/ml
    CV%
    # of assays
    Meanng/ml
    CV%
    12
    1.6
    1.60
    8
    1.6
    3.70
    12
    0.4
    4.05
    8
    0.4
    5.42
    12
    0.1
    4.56
    8
    0.1
    7.59

    Precision is defined as the percent coefficient of variation (%CV). The data above shows CV value of both intra and inter-assay for 3 control samples in low, mid and high concentration range.

    Intra-assay Precision CV<5% and Inter-assay Precision CV<10%

  • Recovery
    Sample Matrix
    Protein A Added (ng/ml)
    % Recovery
    6.20 mg/ml human IgG
    1.50
    85.01
    1.19 mg/ml customer Ab
    1.00
    98.07
    1.13 mg/ml human IgG
    1.20
    108.96
    1.00 mg/ml human IgG
    2.50
    100.01

    The assay was performed by spiking the Protein A standards provided in this kit into the IgG samples. The recovery rates are within 85% to 115%.

Related Products

Cat. No. Product Name Quantity Price Order
A01778
Protein A Antibody, mAb, Mouse
$105.00 40 μg
 
A01779
Protein A Antibody [Biotin], mAb, Mouse
$108.00 40 μg
 
L00210
Protein A Resin
$108.00 5 ml Resin (Total 10 ml)
 
M00091
Streptavidin-HRP
$95.00 1 mg
 

REQUEST A QUOTE

feedback

Do you like the current new website?

Hate

Dislike

Neutral

Like

Love

*