BSA ELISA Kit, 2G utilizes two anti-BSA antibodies which bind to different epitopes of BSA. When the sample and the anti-BSA detection antibody conjugated with biotin are added to the plate (Capture Plate) coated with the anti-BSA capture antibody, the capture antibody and the detection antibody (Biotin Anti-BSA Antibody) bind to the BSA in the sample in a sandwich format. Streptavidin Horseradish Peroxidase conjugate (Streptavidin-HRP) is added to interact with the Biotin Anti-BSA Antibody. After washing steps, 3,3',5,5'-Tetramethylbenzidine solution (TMB Solution) is added, resulting in formation of blue color. The reaction is stopped by adding Stop Solution. Application of the Stop Solution results in the color changing from blue to yellow. The intensity of the color can be read at 450 nm and 650 nm by a microplate reader. The quantity of BSA in the sample is precisely quantified against a BSA standard curve.
Figure 1: Schematic diagram of BSA ELISA Kit, 2G