AAV2 Titer Capsid ELISA Kit is based on the sandwich ELISA method.
When standards (AAV2 Control) or samples are added to the capture plate, the AAV2 capsids in the sample can be captured on the plate coated with the capture antibody. Then the detection antibody (Biotin Anti-AAV2 Antibody) conjugated with biotin is added to interact with the AAV2 capsids bound on the plate. Streptavidin-Horseradish Peroxidase conjugate (Streptavidin-HRP) is added to interact with the biotin conjugated detection antibody. After washing steps, 3,3',5,5'-Tetramethylbenzidine solution (TMB Solution) is added resulting in formation of blue color. The reaction is stopped by adding Stop Solution. Application of the Stop Solution results in the color changing from blue to yellow. The intensity of the color can be read at 450 nm and 650 nm by a microplate reader. The quantity of AAV2 capsids in the sample is precisely quantified against an AAV2 standard curve.
Figure 1: Schematic diagram of AAV2 Titer Capsid ELISA Kit