Resources » Reference Databases » Citations Database

For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.

Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library

STAR Protoc. 2021-07; 
Frances V Hundley, David P Toczyski
Products/Services Used Details Operation
Oligo pool … 3. Amplify the sgRNA oligos (we ordered our pooled sgRNA library from CustomArray Inc.). General sgRNA oligo design (with sgRNA sequence underlined) and universal forward and reverse primers are shown below. UPS sgRNA library oligos and primers … Get A Quote

Abstract

The development of CRISPR-Cas9 screening techniques coupled with chemical inhibition of specific biological processes enables high-throughput investigation into many areas of molecular biology. We present a protocol to conduct ubiquitin proteasome system-specific chemical-genetic CRISPR-Cas9 screens in the human HAP1 cell line. This protocol can be adapted for use in other cell lines, with other compounds and types of treatments, and with any other sgRNA library. For complete details on the use and execution of this protocol, please refer to Hundley et al. (2021).

Keywords

CRISPR, Genetics, High Throughput Screening
Article Link >

Most Popular Services

Homology-Directed Repair (HDR) Knock-in Templates
CRISPR Cas9 Single guide RNA (sgRNA) and Ribonucleoprotein (RNP)
Plasmid DNA Preparation Service
Mammalian CHO Expression Service