Epitope molecularly imprinted polymers based on host-guest interaction: specific recognition of CD59

The detection of CD59 is of great clinical importance since it is an important glycoprotein that can serve as a biomarker related to kinds of cancers. In this work, we prepared host-guest interaction based oriented epitope molecularly imprinted polymer (hg-EMIP) for the immobilization of CD59 N-terminal epitopes. Cucurbit[7]uril (CB[7]) and l-phenylalanine (L-phe) were employed as the host and guest; and tannic acid (TA) with abundant hydroxyl groups and diethylenetriamine (DETA) were chosen as the functional monomer and crosslinking agent, respectively. The obtained hg-EMIP can specifically recognize CD59 with the adsorption capacity of 88.2 mg/g and imprinting factor of 5.63, and possesses high reusability. The hg-EMIP-based method possesses a wide linear range (1 ng/mL - 1 μg/mL) and low limit of detection (0.44 ng/mL), and can be successfully used for the detection of CD59 in human serum sample. This study provides a scheme for the preparation of host-guest based epitope molecularly imprinted polymers for helping to identify potential disease biomarkers efficiently.