Stable intronic sequence RNA (sisRNA): an expanding universe

hy do eukaryotic cells have introns? Our genes are frequently interspersed with noncoding introns that are commonly believed to be “junk” because they are highly unstable after splicing. Recent studies have identified various forms of stable intronic sequence (sis) RNAs (linear and circular) that play regulatory roles in gene expression. Although sisRNAs are conserved from yeast to human, the biogenesis and functions of conserved sisRNAs from invertebrates to vertebrates remain unexplored. In this talk, I will present our latest findings on the roles of sisRNAs from an orthologous intron in Drosophila and human cells. Our study suggests that orthologous introns produce sisRNAs with autoregulatory functions, although evolutionarily distinct biogenesis leads to different modes of action.

Webinar Details

  • Date: 23rd Sept, 2021
  • Time: 2PM SGT
  • Speaker:
Dr. Pek Jun Wei Dr. Pek Jun Wei

Principal Investigator, Temasek Life Science Laboratory, Singapore

Dr. PEK Jun Wei received his B.Sc. (Hons) (2008) and Ph.D. (2011) from the National University of Singapore (NUS). He did his graduate research in the laboratory of Dr. Toshie Kai at the Temasek Life Sciences Laboratory (TLL) where he studied the roles of nuage and small RNAs in the Drosophila germline. In 2012, he joined the laboratories of Drs. Joseph Gall and Allan Spradling at the Carnegie Institution for Science (Department of Embryology) as a Carnegie Collaborative Fellow. He developed a research program to study a novel class of noncoding RNAs (stable intronic sequence RNAs or sisRNAs) in Drosophila. He was later named a Howard Hughes Medical Institute (HHMI) Fellow of the Life Sciences Research Foundation and awarded a fellowship from the Jane Coffin Childs Memorial Fund. He returned to TLL as a Young Investigator in November 2014 to start his independent research group.

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