WoLF PSORT: Advanced Protein Subcellular Localization Prediction Tool

PSORT prediction tool uses amino acid sequence information to reliably predict protein localization sites, based on rules derived from experimental observations. For example, gram negative bacteria have four localization sites: cytoplasm, inner membrane, periplasm and the outer membrane.

• Inner membrane proteins are recognized by the presence of either a hydrophobic stretch in the predicted mature protein or an uncleavable N-terminal signal sequence.
• Lipoproteins are first recognized by a consensus pattern and then assumed present at either the inner or outer membrane. These two possibilities are further dissected by examining an acidic residue in the mature N-terminal portion.
• Periplasmic and outer membrane proteins can be successfully differentiated by their different amino acid compositions.