Antibody Expression Handbook

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Antibodies (Abs) are the fastest growing class of drugs and the reasons for this growth are obvious. Their long half-lives allow for infrequent dosing. They have high specificity for their targets and are well tolerated. Recombinant DNA technology can be utilized to engineer improved binding or effector functions. Given some of these factors, there is a robust increase in the development of human monoclonal Abs for therapeutic purposes. As a result there has been a major shift in the focus of many pharmaceutical companies as they started moving from exclusive small molecule drug discovery to a broader portfolio containing both, chemical entities [small molecules] as well as biotherapeutics [large molecules including antibodies].

This Ab handbook serves to provide a broad overview of the discovery process for generation of Ab-based therapeutics, while discussing basic concepts around Ab production. Discovery topics include target selection, target assessment & validation, screening, lead identification and optimization, leading up to candidate selection.

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GenScript's service capabilities in recombinant antibody (rAb) production are provided where necessary. Glossary of important terms in alphabetical order, along with references and suggestions for further reading, are provided at the end. This concise and colorful resource is available as a free PDF download.

Antibody Drug Discovery Resources and Services

Antibody Discovery

Antibody Discovery: GenScript’s Antibody Engineering group can build antibody library with up to 10¹⁰ individual clones, to speed up your antibody discovery efforts.

Antibody Sequencing

GenScript’s advanced Antibody Sequencing technology offers fast and professional sequencing services for your monoclonal antibodies.

Assays

GenScript has developed several cell-based ADCC/CDC functional assays to profile the efficacy and potency of your therapeutic antibodies using proprietary recombinant effector cells.

Antibody Engineering

GenScript scientists’ extensive experience in antibody engineering can provide superior services such as antibody humanization, affinity maturation and more.

Antibody Production

With solid expertise in recombinant antibody (rAb) production techniques, GenScript provides a comprehensive rAb service portfolio that deliver microgram to gram quantities of pure rAb for each stage of your Ab drug discovery program.

PK/PD Study

PK/PD Study: GenScript offers over 120 tumor and inflammation models for evaluation of in vivo efficacy, PK/PD, biomarker and bioanalysis studies. GenScript Anti-idiotype Antibody services are also a powerful tool for antibody drug PK/PD and immunogenicity studies.

You can also view our Recombinant Antibody Service Selection Guide to identify services that are the best match for your application.