DFNA5 cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	DFNA5
Entrez Gene ID	100477274
Full Name	gasdermin E
Synonyms	DFNA5
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019798630.1	XP_019654189.1	non-syndromic hearing impairment protein 5

Pathways from BioSystems
Gene Ontology

The following DFNA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DFNA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc88222	XM_019798630.1 Latest version!	Ailuropoda melanoleuca DFNA5, deafness associated tumor suppressor (DFNA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DFNA5 ( XM_019798630.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654189.1 Ailuropoda melanoleuca DFNA5, deafness associated tumor suppressor (DFNA5), mRNA.

Price & Availability↑

CloneID	OAc88222
Clone ID Related Accession (Same CDS sequence)	XM_019798630.1
Accession Version	XM_019798630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	non-syndromic hearing impairment protein 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217299.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCCA AAGCAACAAG GAATTTTCTT AAAGAAGTTG ATGCCGGAGG GAACCTGATT 
GCAGTATCAA ACTTGAATGA CTCTGATAAG ATACAGCTTC TAAGTCTAGT GACCAAAAAA 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACAAATTTT TATCTGTCAC CCTCGGTGAT 
GTACTCAGAG AAGACCAATT TCTGAGTCCA GTGGTCGTGG AGTCGGACTT CGTGAAATAC 
GATTATAAGC TTCAAAACCA CGTGAGGGGG ACCATCGAGA CGGCACTAGG GAAGGTCAAG 
CTGAATGTTG GAGGCAAAGG ACTTGTGGAA AGTCAATCTT CTTTTGGAAG ACTGAAGAAG 
CAGGAGGTGG ACCTGCAGCA GCTCATCAGA GACTCTGCTG AGAGAGCAAT CGATCTGAGA 
AACCCAGTGC TGCAGCAGGT GCTGGAGAGG AAGAAGGTGG TCCTGTGTGT CCTGACCCAG 
AAGATAGTGA CGGCGCAGCC ATGTGTGATA TCAGAGCACA CTCAGCTGGA GGAGAAGTGC 
AGCAGCATGG TGGGGATCCC GAGCAAGACC GTGCAGGTGT CTGCAAAGGA AGATGGGAGC 
ATCGTCAAAG ACACCCAAGT GGAGCTGGAG ATCCCGGCTC CCACCACCAT CGCCTATGGC 
GTCATCGAGT TGTATGTGAG ACAGGACGGC CGTTTCAGCT ACAGCTGCAG GCACTCAGAA 
CGTGCACCAT CTCCACGGGA CAGTCGCCAG CTCCTGGACG CACAAGAAGC CAAAGAAGAC 
CCACGTGAGG CATGGGATGA AGTGGTAGAG ATTTCCTCTT CACACGTGGA GCAGCCTGCC 
TTTCACGGAG AATTCTGTCC CCTACAAGGG AATTATTGTG GCTTCGAGCT CAGGAAAAGT 
GGCGCTCTCT TCATGGACTC TCTGCCCTTA CAAGAGTTTG CATTTGGGGA CGTGCCAGAT 
GCTGGGCAGG GACTGCCTGC CCTGGATAGG CCGTTGAGCG TTTTAAAACA AGCCACCCTG 
CTCTTAGAGA GGAATTTCCA TCCCTTTGTG GAGCTGCCAG AAGAGCAGCG GATGGCACTG 
AGCAACATCC TCCAGGTGGT CCTGTCTGAT GAAGAATTAC TTGTGGCCCT GGAGCAAGTG 
TGTGAGGATG TGGTCAACGG CCTGTCGCCC CCACCAGCAA TCCTGGAGAA GCTGAAGCCC 
CCACAGCAGC AGGACCTCAT GGCTTTCCTA TGTCTGGTGG GGTACAGAGT CCAGGGCGGG 
CATCCAGGCC CGGAGGATGC AGTCAGCAAC CAAAAGCTCT TTTCTACGGC CTACTTCTTG 
GTCAGTGCAC TAGCAGAAAT GCCAGCTAAT ACAGTGGCTC TGCTGGGTGC TTGCTGTAAA 
CTCCAGATTA TTCCTATGCT TTGTCACTTG CTCCGTGCTC TTGCTGATGG AGTATCTGAT 
CTTGAAAACC CAGACTTGGC TCCTCTGAAA GATACGGAAA GGTTTACGAT CGTGCAGCAT 
TTGTTTGCCT CAGTCGACAT GACCCTGGAG AGAGAGCAGT CATCCGTGAA AGTTGTCACC 
CAGAAGGACC CTAACATTTC CCCACTCATT CTTTACATAA GCCTGAATGG TTTCTGTGCT 
TTAGGCAGAG CACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654189.1
CDS	60..1697
Translation	MFAKATRNFLKEVDAGGNLIAVSNLNDSDKIQLLSLVTKKKRFWCWQRPKYKFLSVTLGDVLREDQFLSPVVVESDFVKYDYKLQNHVRGTIETALGKVKLNVGGKGLVESQSSFGRLKKQEVDLQQLIRDSAERAIDLRNPVLQQVLERKKVVLCVLTQKIVTAQPCVISEHTQLEEKCSSMVGIPSKTVQVSAKEDGSIVKDTQVELEIPAPTTIAYGVIELYVRQDGRFSYSCRHSERAPSPRDSRQLLDAQEAKEDPREAWDEVVEISSSHVEQPAFHGEFCPLQGNYCGFELRKSGALFMDSLPLQEFAFGDVPDAGQGLPALDRPLSVLKQATLLLERNFHPFVELPEEQRMALSNILQVVLSDEELLVALEQVCEDVVNGLSPPPAILEKLKPPQQQDLMAFLCLVGYRVQGGHPGPEDAVSNQKLFSTAYFLVSALAEMPANTVALLGACCKLQIIPMLCHLLRALADGVSDLENPDLAPLKDTERFTIVQHLFASVDMTLEREQSSVKVVTQKDPNISPLILYISLNGFCALGRAH

Target ORF information:

RefSeq Version	XM_019798630.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca DFNA5, deafness associated tumor suppressor (DFNA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019798630.1

ORF Insert Sequence:

ATGTTTGCCA AAGCAACAAG GAATTTTCTT AAAGAAGTTG ATGCCGGAGG GAACCTGATT 
GCAGTATCAA ACTTGAATGA CTCTGATAAG ATACAGCTTC TAAGTCTAGT GACCAAAAAA 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACAAATTTT TATCTGTCAC CCTCGGTGAT 
GTACTCAGAG AAGACCAATT TCTGAGTCCA GTGGTCGTGG AGTCGGACTT CGTGAAATAC 
GATTATAAGC TTCAAAACCA CGTGAGGGGG ACCATCGAGA CGGCACTAGG GAAGGTCAAG 
CTGAATGTTG GAGGCAAAGG ACTTGTGGAA AGTCAATCTT CTTTTGGAAG ACTGAAGAAG 
CAGGAGGTGG ACCTGCAGCA GCTCATCAGA GACTCTGCTG AGAGAGCAAT CGATCTGAGA 
AACCCAGTGC TGCAGCAGGT GCTGGAGAGG AAGAAGGTGG TCCTGTGTGT CCTGACCCAG 
AAGATAGTGA CGGCGCAGCC ATGTGTGATA TCAGAGCACA CTCAGCTGGA GGAGAAGTGC 
AGCAGCATGG TGGGGATCCC GAGCAAGACC GTGCAGGTGT CTGCAAAGGA AGATGGGAGC 
ATCGTCAAAG ACACCCAAGT GGAGCTGGAG ATCCCGGCTC CCACCACCAT CGCCTATGGC 
GTCATCGAGT TGTATGTGAG ACAGGACGGC CGTTTCAGCT ACAGCTGCAG GCACTCAGAA 
CGTGCACCAT CTCCACGGGA CAGTCGCCAG CTCCTGGACG CACAAGAAGC CAAAGAAGAC 
CCACGTGAGG CATGGGATGA AGTGGTAGAG ATTTCCTCTT CACACGTGGA GCAGCCTGCC 
TTTCACGGAG AATTCTGTCC CCTACAAGGG AATTATTGTG GCTTCGAGCT CAGGAAAAGT 
GGCGCTCTCT TCATGGACTC TCTGCCCTTA CAAGAGTTTG CATTTGGGGA CGTGCCAGAT 
GCTGGGCAGG GACTGCCTGC CCTGGATAGG CCGTTGAGCG TTTTAAAACA AGCCACCCTG 
CTCTTAGAGA GGAATTTCCA TCCCTTTGTG GAGCTGCCAG AAGAGCAGCG GATGGCACTG 
AGCAACATCC TCCAGGTGGT CCTGTCTGAT GAAGAATTAC TTGTGGCCCT GGAGCAAGTG 
TGTGAGGATG TGGTCAACGG CCTGTCGCCC CCACCAGCAA TCCTGGAGAA GCTGAAGCCC 
CCACAGCAGC AGGACCTCAT GGCTTTCCTA TGTCTGGTGG GGTACAGAGT CCAGGGCGGG 
CATCCAGGCC CGGAGGATGC AGTCAGCAAC CAAAAGCTCT TTTCTACGGC CTACTTCTTG 
GTCAGTGCAC TAGCAGAAAT GCCAGCTAAT ACAGTGGCTC TGCTGGGTGC TTGCTGTAAA 
CTCCAGATTA TTCCTATGCT TTGTCACTTG CTCCGTGCTC TTGCTGATGG AGTATCTGAT 
CTTGAAAACC CAGACTTGGC TCCTCTGAAA GATACGGAAA GGTTTACGAT CGTGCAGCAT 
TTGTTTGCCT CAGTCGACAT GACCCTGGAG AGAGAGCAGT CATCCGTGAA AGTTGTCACC 
CAGAAGGACC CTAACATTTC CCCACTCATT CTTTACATAA GCCTGAATGG TTTCTGTGCT 
TTAGGCAGAG CACATTAA