Overview

Stable isotope labeled peptides have long been a staple of NMR spectroscopy studies. GenScript can provide custom peptides labeled with stable isotopes including 2H (deuterium), 15N, 13C, or a combination of 15N and 13C. Stable isotope labeled peptides can be useful for the determination of protein structure and dynamics or protein-protein interactions, by allowing for the incorporation of NMR active nuclei which can help reduce the complexity of spectra, and help scientists obtain new correlations between atoms for more complete structural information.

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*Label options

2H (deuterium)
15N
13C
15N and 13C combinations

*Stable isotope labels can be incorporated in combination with any of our peptide modifications

To request a quote or order: Deliverables:

To request a quote, use our online ordering system or send an email to [email protected] indicating:

  • Your peptide sequence including label location and type, and any additional desired modifications
  • Your desired peptide quantity
  • Your desired peptide purity
  • Lyophilized isotopically labeled peptide of specified quantity and purity
  • QC reports including:
    -HPLC chromatogram
    -MS spectra
    -Certificate of Analysis
  • ArgonShield™ protective packaging

Application

Nuclear magnetic resonance (NMR) spectroscopy

NMR spectroscopy is a key tool in structural biology research, allowing for the characterization of protein structures, protein dynamics, and protein-protein interactions. It is advantageous over X-ray crystallography, in that the structures of proteins or peptides in liquid can be solved. Additionally, recent advances in NMR spectroscopy have demonstrated its value in drug discovery, due to its ability to help researchers directly identify ligand binding sites.

The technique involves subjecting a sample to a magnetic field, taking advantage of an intrinsic property of certain atomic nuclei, called spin. In response to the magnetic field, nuclear spins flip from low energy spin states to higher energy states. As the spins flip, energy is absorbed by the nucleus. Absorption spectra data can be used to identify atomic nuclei and the distance between nuclei.

Stable isotopic labeling of peptides allows for the incorporation of NMR active nuclei which can help reduce the complexity of spectra and help scientists obtain new correlations between atoms, for more complete structural information.

Selected publications citing stable isotope labeled peptides synthesized by GenScript

books Pustovalova Y. et al. (2012) The C-terminal domain of human Rev1 contains independent binding sites for DNA polymerase Η and Rev7 subunit of polymerase Ζ. FEBS Lett. 586: 3051-3056.

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