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Analysis of single nucleotide polymorphism in human angiogenin using droplet-based microfluidics.

BioChip Journal.. 2014-03;  8(1):15-21
Nirmala B,Jae-Won C,Soo-Ik C. Department of Biochemistry, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea.
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Human angiogenin through its ribonucleolytic activity and nuclear translocation promotes angiogenesis and protects motor neurons from damage under stress conditions. Several heterozygous missense and loss of function mutations in the coding region of angiogenin have been implicated in the progression of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Every year, thousands of new patients of ALS are diagnosed, making detection of angiogenin variants an urgent necessity. In this work, we report a method of SNP detection based on offline PCR amplification of the target samples followed by bulk and droplet-based microfluidic fluorescence measurements. Our assay relied on the 5′ nucleas... More


Angiogenin; Single nucleotide polymorphism; Amyotrophic lateral sclerosis; Droplet-based microfluidics; TaqMan MGB probe