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6xHistidine and Glutathione S-transferase (GST) are commonly used protein/peptide tags added to the end of a recombinant protein to aid in their purification or detection. GenScript Ni-Charged MagBeads and Glutathione MagBeads provide simple, rapid and reliable methods for micro-scale purification of His- and GST-tagged fusion proteins from crude cell lysate prepared from bacteria, yeast or mammalian cell culture.
The Ni-Charged MagBeads are pre-charged with nickel and ready to use for micro-scale purification of 6xHis-tagged proteins under native or denaturing conditions. The Glutathione MagBeads are immobilized with Glutathione mainly through its central carbon on the beads surface for efficient GST binding. Read More »
All the protocols are easily performed by applying the magnetic separation technology. They do not require the use of columns or centrifugation and all the steps take place in a single tube. The MagBeads can be used in very small volumes to acquire high concentrated target proteins. All the advantages make Ni-Charged MagBeads and Glutathione MagBeads the ideal tools for micro-scale purification of His- and GST- tagged fusion protein.
Lane 1, Bacterial lysate expressing GST Tag.Lane 2, Purified GST Tag (26kDa) using Glutathione MagBeads.Lane 3, Bacterial lysate expressing His-tagged protein.Lane 4, Purified His-tagged protein (27kDa) using Ni-charged MagBeads.
Fig.2. Purification of GST Tag and His-tagged Protein using GenScript Micro-scale Tagged Protein Purification MagBeads. Lane M, Protein Molecular Weight Standard.
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