| Matrix |
Highly crosslinked 4% beaded agarose |
Agarose, 4% |
Agarose, 4% crosslinked |
Highly crosslinked 4% beaded agarose |
Agarose, 4% crosslinked |
Agarose, 4% crosslinked |
| Average bead size |
~ 90 um |
~ 90 um |
~ 90 um |
~ 90 um |
~ 90 um |
|
| Ligand |
Alkali-Tolerant Protein A |
Recombinant Streptococcal protein A expressed in
E. coli |
Recombinant Streptococcal protein A expressed in
E. coli |
Recombinant Streptococcal protein A expressed in
E. coli |
Recombinant Streptococcal Protein G lacking the albumin-binding produced
in
E. coli |
Highly purified protein L |
| Binding Capacity |
> 30 mg hIgG/ml resin |
> 20 mg human IgG/ml resin |
30 - 40 mg hIgG /ml settled resin |
> 40 mg rabbit IgG/ml settled resin |
>20 mg sheep IgG/ml settled resin |
>15 mg rabbit Ig/ml settled resin |
| Chemical Stability |
0.5 M NaOH for CIP
Stable with all commonly used reagents
|
Stable with all commonly used reagents |
Stable with all commonly used reagents |
Stable with all commonly used reagents |
Stable with all commonly used reagents |
Stable with all commonly used reagents |
| Working pH |
3-12 |
3-9 |
3-9 |
3-9 |
3-9 |
2 to 10 |
| Clean in place |
0.1-0.5 M NaOH |
6 M guanidine hydrochloride
0.1% Triton X-100
Alternatively, wash the column with 70% ethanol
|
6 M guanidine hydrochloride
0.1% Triton X-100
Alternatively, wash the column with 70% ethanol
|
6 M guanidine hydrochloride
0.1% Triton X-100
Alternatively, wash the column with 70% ethanol
|
0.1% Triton X-100
Alternatively, wash the column with 70% ethanol
|
Washing of the packed column with 70% ethanol
15 mM NaOH
|
| Linear flow velocity |
50-300 cm/h |
50-300 cm/h |
50-300 cm/h |
50-300 cm/h |
50-300 cm/h |
50-300 cm/h |
| Storage |
20% ethanol, 2 - 8°C |
20% ethanol, 2 - 8°C |
1X PBS containing 20% ethanol, 2 - 8°C |
1X PBS containing 20% ethanol, 2 - 8°C |
1X PBS containing 20% ethanol, 2 - 8°C |
1X PBS containing 20% ethanol, 2 - 8°C |
