GenScript offers recombinant protein A with non-essential domains removed, recombinant protein G with albumin binding site deleted as well as codon optimized protein L. By pre-coupling these genetically modified proteins to 4% highly cross-linked agarose, GenScrtipt developed excellent antibody affinity chromatography matrix - Protein A, G and L Resins for batch/gravity purification of polyclonal and monoclonal antibody from cell culture supernatants, serum, and ascites at both laboratory and larger scale. With enhanced binding capacity, these resins enable you to get highly purified antibodies with minimal contaminations, resulting in cleaner preparations and greater yields.
Protein A, protein G and protein L are surface proteins originally found in cell wall of bacteria Staphylococcus aureus, Group G Streptococci and Peptostreptococcus magnus, respectively. Protein A and protein G bind with high affinity and specificity to Fc region of immunoglobulins from many of mammalian species, most notably IgGs. Unlike protein A and G, Protein L binds immunoglobulins through kappa light chain. These proteins have been usually used to purify, immobilize or detect immunoglobulins.
| Cat. No. | Product Name | Quantity | Price | Order |
|---|---|---|---|---|
|
L00209
|
Protein G Resin |
|
$129.00 5 ml Resin (Total 10 ml) |
|
|
L00210
|
Protein A Resin |
|
$107.80 5 ml Resin (Total 10 ml) |
|
|
L00239
|
Protein L Resin |
|
$159.50 2.5 ml Resin (Total 5 ml) |
|
|
L00433
|
Monofinity A Resin |
|
$1680.00 |
|
|
L00464
|
Protein A Resin FF |
|
$401.70 5 ml Resin (Total 10 ml) |
|
|
L00664
|
Protein G Resin FF |
|
$428.48 |
|
|
L00680
|
Protein A Resin FF Prepacked Column |
|
$251.00 |
|
|
L00681
|
Protein G Resin FF Prepacked Column |
|
$411.00 |
|
Table 1. Properties of the recombinant Protein A, G and L on Resins
| Protein A | Protein G | Protein L | |
|---|---|---|---|
| Source | Staphylococcus aureus | Streptococcus | Peptostreptoccus magnus |
| Molecular weight | 34 kDa | 22 kDa | 42 kDa |
| Numbers of IgG(Ig) binding sites | 5 | 3 | 5 |
| IgG Binding capacity | >20 mg human IgG/ml settled resin | >20 mg sheep IgG per ml settled resin | >15 mg rabbit Ig per ml settled resin |
Selection Guide of Protein A, G and L Resins
| Monofinity A | Protein A Resin | Ultra Protein A Resin | Protein A Resin FF | Protein G Resin | Protein L Resin | |
|---|---|---|---|---|---|---|
| Matrix | Highly crosslinked 4% beaded agarose | Agarose, 4% | Agarose, 4% crosslinked | Highly crosslinked 4% beaded agarose | Agarose, 4% crosslinked | Agarose, 4% crosslinked |
| Average bead size | ~ 90 um | ~ 90 um | ~ 90 um | ~ 90 um | ~ 90 um | |
| Ligand | Alkali-Tolerant Protein A | Recombinant Streptococcal protein A expressed in E. coli |
Recombinant Streptococcal protein A expressed in E. coli |
Recombinant Streptococcal protein A expressed in E. coli |
Recombinant Streptococcal Protein G lacking the albumin-binding produced
in E. coli |
Highly purified protein L |
| Binding Capacity | > 30 mg hIgG/ml resin | > 20 mg human IgG/ml resin | 30 - 40 mg hIgG /ml settled resin | > 40 mg rabbit IgG/ml settled resin | >20 mg sheep IgG/ml settled resin | >15 mg rabbit Ig/ml settled resin |
| Chemical Stability |
0.5 M NaOH for CIP Stable with all commonly used reagents |
Stable with all commonly used reagents | Stable with all commonly used reagents | Stable with all commonly used reagents | Stable with all commonly used reagents | Stable with all commonly used reagents |
| Working pH | 3-12 | 3-9 | 3-9 | 3-9 | 3-9 | 2 to 10 |
| Clean in place | 0.1-0.5 M NaOH |
6 M guanidine hydrochloride 0.1% Triton X-100 Alternatively, wash the column with 70% ethanol |
6 M guanidine hydrochloride 0.1% Triton X-100 Alternatively, wash the column with 70% ethanol |
6 M guanidine hydrochloride 0.1% Triton X-100 Alternatively, wash the column with 70% ethanol |
0.1% Triton X-100 Alternatively, wash the column with 70% ethanol |
Washing of the packed column with 70% ethanol 15 mM NaOH |
| Linear flow velocity | 50-300 cm/h | 50-300 cm/h | 50-300 cm/h | 50-300 cm/h | 50-300 cm/h | 50-300 cm/h |
| Storage | 20% ethanol, 2 - 8°C | 20% ethanol, 2 - 8°C | 1X PBS containing 20% ethanol, 2 - 8°C | 1X PBS containing 20% ethanol, 2 - 8°C | 1X PBS containing 20% ethanol, 2 - 8°C | 1X PBS containing 20% ethanol, 2 - 8°C |
