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Protein Purification Resins

Technical Resources

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Protein A, G and L Resins

Simplify Your Antibody Purifiction Tasks

Protein A, G and L Resins

GenScript offers recombinant protein A with non-essential domains removed, recombinant protein G with albumin binding site deleted as well as codon optimized protein L. By pre-coupling these genetically modified proteins to 4% highly cross-linked agarose, GenScrtipt developed excellent antibody affinity chromatography matrix - Protein A, G and L Resins for batch/gravity purification of polyclonal and monoclonal antibody from cell culture supernatants, serum, and ascites at both laboratory and larger scale. With enhanced binding capacity, these resins enable you to get highly purified antibodies with minimal contaminations, resulting in cleaner preparations and greater yields.

Highlights



Products

Protein A, protein G and protein L are surface proteins originally found in cell wall of bacteria Staphylococcus aureus, Group G Streptococci and Peptostreptococcus magnus, respectively. Protein A and protein G bind with high affinity and specificity to Fc region of immunoglobulins from many of mammalian species, most notably IgGs. Unlike protein A and G, Protein L binds immunoglobulins through kappa light chain. These proteins have been usually used to purify, immobilize or detect immunoglobulins.

Ordering Information

Cat. No.
Product Name
Price
L00433-55
 Monofinity A Resin Prepacked Column 5mlx5 - 5 ml prepacked x 5
$1809.00
L00433-51
 Monofinity A Resin Prepacked Column 5mlx1 - 5 ml prepacked x 1
$499.00
L00433-5
 Monofinity A Resin - 5 L
L00433-25
 Monofinity A Resin - 25 ml
$1680.00
L00433-200
 Monofinity A Resin - 200 ml
$3350.00
L00433-15
 Monofinity A Resin Prepacked Column 1mlx5 - 1 ml prepacked x 5
$549.00
L00433-11
 Monofinity A Resin Prepacked Column 1mlx1 - 1 ml prepacked x 1
$169.00
L00433-10
 Monofinity A Resin - 10 L
L00433-1
 Monofinity A Resin - 1 L
L00210
 Protein A Resin - 5 ml
$98.00
L00464-5
 Protein A Resin FF - 5 ml
$390.00
L00209
 Protein G Resin - 5 ml
$129.00
L00239
 Protein L Resin - 2.5 ml
$145.00
L00664-5
 Protein G Resin FF - 5 ml
$416.00
L00664-25
 Protein G Resin FF - 25 ml
$1473.00
L00664-100
 Protein G Resin FF - 100 ml
$2209.00
L00680-55
 Protein A Resin FF Prepacked Column - 5 ml x 5
$2083.00
L00680-51
 Protein A Resin FF Prepacked Column - 5 ml x 1
$465.00
L00680-12
 Protein A Resin FF Prepacked Column - 1 ml x 2
$251.00
L00681-55
 Protein G Resin FF Prepacked Column - 5 ml x 5
$3410.00
L00681-51
 Protein G Resin FF Prepacked Column - 5 ml x 1
$761.00
L00681-12
 Protein G Resin FF Prepacked Column - 1 ml x 2
$411.00
 

Table 1. Properties of the recombinant Protein A, G and L on Resins

Protein A Protein G Protein L
Source Staphylococcus aureus Streptococcus Peptostreptoccus magnus
Molecular weight 34 kDa 22 kDa 42kDa
Numbers of IgG(Ig) binding sites 5 3 5
IgG Binding capacity >20 mg human IgG/ml settled resin >20 mg sheep IgG per ml settled resin >15 mg rabbit Ig per ml settled resin

Table 2. Selection Guide of Protein A, G and L Resins

 
Monofinity A
Protein A Resin
Ultra Protein A Resin
Protein A Resin FF
Protein G Resin
Protein L Resin
Matrix
Highly crosslinked 4% beaded agarose
Agarose, 4%
Agarose, 4% crosslinked
Highly crosslinked 4% beaded agarose
Agarose, 4% crosslinked
Agarose, 4% crosslinked
Average bead size
~ 90um
~ 90um
~ 90um
~ 90um
~ 90um
~ 90um
Ligand
Alkali-Tolerant Protein A
Recombinant Streptococcal protein A expressed in
E. coli
Recombinant Streptococcal protein A expressed in
E. coli
Recombinant Streptococcal protein A expressed in
E. coli
Recombinant Streptococcal Protein G lacking the albumin-binding produced in
E. coli
Highly purified protein L
Binding Capacity
> 30 mg hIgG/ml resin
> 20 mg human IgG/ml resin
30 - 40 mg hIgG /ml settled resin
> 40 mg rabbit IgG/ml settled resin
>20 mg sheep IgG/ml settled resin
>15 mg rabbit Ig/ml settled resin
Chemical Stability
0.5M NaOH for CIP
Stable with all commonly used reagents
Stable with all commonly used reagents
Stable with all commonly used reagents
Stable with all commonly used reagents
Stable with all commonly used reagents
Stable with all commonly used reagents
Working pH
3-12
3-9
3-9
3-9
3-9
2 to 10
Clean in place
0.1-0.5M NaOH
6 M guanidine hydrochloride
0.1% Triton™ X-100
Alternatively, wash the column with 70% ethanol
6 M guanidine hydrochloride
0.1% Triton™ X-100
Alternatively, wash the column with 70% ethanol
6 M guanidine hydrochloride
0.1% Triton™ X-100
Alternatively, wash the column with 70% ethanol
0.1% Triton™ X-100
Alternatively, wash the column with 70% ethanol
Washing of the packed column with 70% ethanol
15mM NaOH
Linear flow velocity
50-300cm/h
50-300cm/h
50-300cm/h
50-300cm/h
50-300cm/h
50-300cm/h
Storage
20% ethanol, 2 - 8°C
20% ethanol, 2 - 8°C
1X PBS containing 20% ethanol, 2 - 8°C
1X PBS containing 20% ethanol, 2 - 8°C
1X PBS containing 20% ethanol, 2 - 8°C
1X PBS containing 20% ethanol, 2 - 8°C
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