Reusable Alkaline-tolerant Resin

Key Features

Novel Protein A ligand withstands CIP procedures with 0.1 to 0.5 M NaOH.

Easy CIP, reduces the contamination and impurity level.

Consistent performance with extremely low ligand leakage.

Dynamic binding capacity over 30 mg/ml under experimental condition.

Can be used for commercial manufacture of monoclonal antibodies.

Significantly reduces overall process costs.

Free sample of 1 ml or 5 ml snap column is available upon request via [email protected].

Product List

Ordering information

Selection Guide

In the biopharmaceutical industry, in order to ensure high quality of products, it is often necessary to carry out clean-in-place (CIP) of chromatography systems. While the column efficiency and transfer rate of the column are also reduced due to the irreversible precipitation of denatured proteins, lipids, and DNA molecules in the sample.

0.1-1 N NaOH can effectively solve the above problems. In addition to sterilizing and inactivating viruses, it can also dissolve denatured proteins, lipids, and DNA molecules deposited on the top of the column, to extend the service life chromatography media. However, natural or recombinant protein A ligands are not tolerant to NaOH, easily getting denatured or shed.

Alkaline-tolerant Protein A Affinity Chromatography Media (Monofinity A Resin, Cat. No. L00433) is alkali-resistant Protein A affinity purification media developed by Genscript to meet the market needs. The product is made of specially engineered alkali-resistant Protein A ligand cross-linked to a novel agarose gel matrix. It has high dynamic binding capacity and low level of ligand shedding, which is suitable for purification of industrial-grade monoclonal antibodies and therapeutic Fc-Fusion proteins.

Specification

Applications

• Example
  

  Lane M: Protein Standard
  Lane 1: Purified antibody under reducing conditions
  Lane 2: Purified antibody under non-reducing conditions
  

  Figure 1. Electrophoresis of humanized IgG purified from cell culture supernatant by Monofinity A Resin

• Dynamic Binding Capacity Performance
  

  Figure 2. Dynamic binding capacity (DBC) versus sample concentration

  

  Figure 3. Dynamic binding capacity (DBC) versus residence time

  

  Figure 4. Breakthrough curve with 5mg/ ml hIgG

• Protein A Leaching
  

  Figure 5. Protein A leakage measured at different cycles of CIP (4 batches)

• Stability & Storage
  

  Figure 6. Dynamic binding capacity stability of Monofinity A Resin for 200 cycles of CIP (0.1M-0.5 M NaOH)

  

  Figure 7. Shelf life of Monofinity A Resin under different storage conditions