Process Scale Antibody Purification
GenScript Monofinity A Resin
GenScript's Monofinity A Resin™ is made of a novel recombinant protein A ligand that has a high specific binding capacity to the Fc region of immunoglobulin molecules and enhances chemical stability under Cleaning-in-Place (CIP) conditions. Monofinity A Resin™ has several benefits such as high-flow agarose base, low non-specific binding and better dynamic binding capacity than similar products from other major vendors.
Free sample of 1 ml or 5 ml snap column is available upon request via
Monofinity A Resin™
Protein A affinity chromatography resin is the most commonly applied tool for the isolation and purification of IgG. Protein A is a cell wall component found in several strains of Staphylococcus aureus . It has five high affinity binding sites capable of binding specifically to the Fc region of immunoglobulin molecules from several species. Covalently immobilized Protein A matrices have been extensively used to purify IgG from several species of mammals.
Dynamic Binding Capacity Performance
Figure 2. Dynamic binding capacity (DBC) versus sample concentration
Figure 3. Dynamic binding capacity (DBC) versus residence time
Figure 4. Breakthrough curve with 5mg/ ml hIgG
Protein A Leaching
Figure 5. Protein A leakage measured at different cycles of CIP (4 batches)
Stability & Storage
Figure 6. Dynamic binding capacity stability of Monofinity A Resin for 200 cycles of CIP (0.1M-0.5 M NaOH)
Figure 7. Shelf life of Monofinity A Resin under different storage conditions