Overview

GenScript's Monofinity A Resin is made of a novel recombinant protein A ligand that has a high specific binding capacity to the Fc region of immunoglobulin molecules and enhances chemical stability under Cleaning-in-Place (CIP) conditions.

GenScript Monofinity A Resin

Protein A affinity chromatography resin is the most commonly applied tool for the isolation and purification of IgG. Protein A is a cell wall component found in several strains of Staphylococcus aureus . It has five high affinity binding sites capable of binding specifically to the Fc region of immunoglobulin molecules from several species. Covalently immobilized Protein A matrices have been extensively used to purify IgG from several species of mammals.

Key Features

Superior Specification

Novel Protein A ligand withstands CIP procedures with 0.1 to 0.5 M NaOH.

Superior Sensitivity

Easy CIP, reduces the contamination and impurity level.

Improved Reactivity

Consistent performance with extremely low ligand leakage.

Wide Applications

Dynamic binding capacity over 30 mg/ml under experimental condition.

Long-term Stability

Can be used for commercial manufacture of monoclonal antibodies.

Long-term Stability

Significantly reduces overall process costs.

Free sample of 1 ml or 5 ml snap column is available upon request via freesample@genscript.com.

Product List

Ordering information

Cat. No. Product Name Quantity Price Order
L00433
Monofinity A Resin
$1680.00
 

Selection Guide

In the biopharmaceutical industry, in order to ensure high quality of products, it is often necessary to carry out clean-in-place (CIP) of chromatography systems. While the column efficiency and transfer rate of the column are also reduced due to the irreversible precipitation of denatured proteins, lipids, and DNA molecules in the sample.

0.1-1 N NaOH can effectively solve the above problems. In addition to sterilizing and inactivating viruses, it can also dissolve denatured proteins, lipids, and DNA molecules deposited on the top of the column, to extend the service life chromatography media. However, natural or recombinant protein A ligands are not tolerant to NaOH, easily getting denatured or shed.

Alkaline-tolerant Protein A Affinity Chromatography Media (Monofinity A Resin, Cat. No. L00433) is alkali-resistant Protein A affinity purification media developed by Genscript to meet the market needs. The product is made of specially engineered alkali-resistant Protein A ligand cross-linked to a novel agarose gel matrix. It has high dynamic binding capacity and low level of ligand shedding, which is suitable for purification of industrial-grade monoclonal antibodies and therapeutic Fc-Fusion proteins.

Specification

Product Name
Monofinity A Resin
Composition
Highly cross-linked 4% agarose 
Average Particle Size
90 μm
Ligand
Alkali-tolerant recombinant protein A (E. coli)
Dynamic Binding
Capacity (DBC)
30 mg human lgG/ ml
(4 min RT with sample hIgG concentration at 5 mg/ ml)
Ligand Leakage
<10 ng/ mg
Pressure/Flow Specification
0.3 MPa/ ~1200 cm/ h
Recommended Flow Rate
50 to 300 cm/ h
Chemical Stability
Stable in all commonly used buffers
pH Working Range
3-12
CIP Stability
0.1-0.5 M NaOH
Storage
20% Ethanol, 2-8℃

Applications

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