ToxinSensor Chromogenic LAL Endotoxin Assay Kit: The kit utilizes a modified Limulus Amebocyte Lysate and a synthetic color producing substrate to quantitatively detect endotoxin chromogenically in a broad range (0.01 to 1 EU/ml).
Ordering Information
Cat. No. | Product Name | Quantity | Price | Order |
---|---|---|---|---|
L00350
|
ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit |
|
$199.00 |
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Technical Advantages
Under standard conditions, absorbance at 545 nm shows a linear relationship with concentrations in the range of 0.01 - 0.1 EU/ml. The above plot illustrates the absorbance (x-axis) of four standards with their corresponding endotoxin concentration (EU/ml) on the y-axis. A best-fit straight line is drawn among these points to graphically determine the endotoxin concentrations of samples based on their absorbance.
Examples using following data
Vial No. | Sample | Absorbance at 545 nm | Δ Absorbance |
---|---|---|---|
1 |
LAL reagent water (Blank) | 0.0525 | - |
2 |
0.01 EU/ml Standard | 0.0965 | 0.0441 |
3 |
0.025 EU/ml Standard | 0.1650 | 0.1125 |
4 |
0.05 EU/ml Standard | 0.3139 | 0.2614 |
5 |
0.1 EU/ml Standard | 0.6702 | 0.6177 |

If the mean absorbance of a sample is x, the endotoxin concentration of sample will be 0.1538x+0.0064 EU/ml. Above is only an example curve show, and the OD values of standards may be different with different assays.