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Protein A, G and A/G MagBeads

Protein A, G or A/G MagBeads Workflow

GenScript Protein A, G and A/G MagBeads are developed for quick, convenient and reproducible immunoprecipitation(IP) and small scale purification of immunoglobulin. By covalently pre-coupling recombinant Protein A, G and A/G to the surface of superparamagnetic beads, these MagBeads provide users simple, easy and time-saving procedures for immunomagnetic separation.

Learn more about Immunoprecipitation (IP) and Co-IP »

Due to the elimination of non-IgG binding domains and other unknown function fragments, the recombinant Protein A, G and A/G both display high affinity for subclasses of IgG from many species including human, rabbit and mouse etc. This permits the purification of IgGs from cell culture supernatants, serum and ascites or other starting samples. In addition, Protein A, G and A/G MagBeads can also be used to immunoprecipitate target proteins from crude samples using a selected primary antibody. To prevent co-elution of the antibody, it is necessary to chemically cross-link the antibody to protein A, G and A/G coated beads prior to the immunoprecipitation steps.

By applying magnetic separation technology, the Protein A, G and A/G MagBeads supply researchers with fast, easy and efficient IP/isolation procedures. A magnetic separation rack is also needed to use the MagBeads. All steps take place in a single tube, without the need for columns, centrifugation, or pre-treatment. Extremely gentle magnetic handling prevents loss of your target IgGs or protein and keeps them intact throughout the procedure. Protein A, G, and G/G MagBeads are the perfect tools for immunoprecipitation and small-scale IgG purification.

                                          Table 1. Properties of the recombinant Protein A, G and L on Resins

  Protein A Protein G Protein A/G
Source Staphylococcus aureus Streptococcus  
Molecular weight 34 kDa 22 kDa 43kDa
Numbers of IgG(Ig) binding sites 5 3 4 of Protein A and 2 of Protein G
IgG Binding capacity 10 mg rabbit IgG per ml settled beads 10 mg goat IgG per ml settled beads 10 mg goat IgG per ml settled beads
Fig.2. Purification of Rabbit IgG from Rabbit serum and Immunoprecipitation of His-Tagged protein from bacterial lysate using GenScript Protein A MagBeads. Lane M, Protein Molecular Weight Standard. Lane 1, Flowthrough of rabbit serum. Lane 2, Purified rabbit IgG from serum. Lane 3, Bacterial lysate expressing His-tagged protein. Lane 4, Immunoprecipitated His-tagged protein, 32kDa (Anti-His antibody cross-linked to the MagBeads).
 

Ordering Information

Cat. No.
Product Name
Size
Price
Quantity
L00273 Protein A MagBeads
4 ml
$89.00
L00672-4 Protein A MagBeads MX
4 ml
$459.00
L00274 Protein G MagBeads
2 ml
$125.00
L00673-4 Protein G MagBeads MX
4 ml
$483.00
L00277 Protein A/G MagBeads
2 ml
$148.00
 


  Related Products
  Tagged Protein Purification MagBeads: micro-scale purification of His, GST and biotin tagged proteins.
  Epitope Tag Antibody Coated MagBeads: Immunoprecipitation and fast isolation of tagged proteins.
  Magnetic Separation Racks: well-designed and practicable device in bio-magnetic separation technology.
 
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