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Purification of recombinant human thyroid peroxidase (hTPO) from AD293 mammalian cells.

Int J Biol Macromol.. 2018-01; 
Kaur P, Patil H, Bhanushali PB, Badgujar SB, Gupta AK.
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Peptide Synthesis ... with various vendors. Codon optimized cDNA sequence of mature peptide of TPO (Uniprot accession: P07202) and sequence containing Gluc gene was synthesized from Genscript (Honkong, China). Restriction endonuclease ... Get A Quote

Abstract

Human thyroid peroxidase (hTPO) has been secretory expressed in AD293 mammalian cells. cDNA sequence of 'Gluc' (Gaussia luciferase) protein from Gaussia princeps was incorporated at the amino terminal of hTPO gene for secretion of targeted protein outside the mammalian cells. Augmentation of TPO clone in serum free mediums was investigated and a simplified purification procedure of hTPO has been reported here. Purified hTPO was further analyzed by SDS-PAGE and immunoblotting (western blotting). The relative molecular mass of hTPO was found to be 105kDa. This is the first report with respect to cost effective and simplified purification approach to get highest yield and purity of recombinant hTPO.

Keywords

Augmentation; Gaussia luciferase; Recombinant hTPO protein
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