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Tuning CRISPR-Cas9 Gene Drives in Saccharomyces cerevisiae

G3 (Bethesda, Md.). 2018-03; 
Roggenkamp E, Giersch RM, Schrock MN, Turnquist E, Halloran M, Finnigan GC
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Gene Synthesis The Cas9 gene was synthesized de novo with a yeast codon bias (Genscript a PCR template) were tested by diagnostic PCR and Sanger DNA sequencing (Genscript) to generate GFY sequence (79 bp), and SUP4 terminator (20 bp) were synthesized de novo (Genscript), subcloned into the generate all sgRNA variants Get A Quote

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