n4bp2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	n4bp2
Entrez Gene ID	100493484
Full Name	NEDD4 binding protein 2
Synonyms	b3bp
General protein information	Preferred Names NEDD4 binding protein 2 Names NEDD4-binding protein 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933469.4	XP_002933515.2	NEDD4-binding protein 2 isoform X1
XM_018095397.1	XP_017950886.1	NEDD4-binding protein 2 isoform X2
XM_018095397.2	XP_017950886.1	NEDD4-binding protein 2 isoform X2
XM_002933469.5	XP_002933515.2	NEDD4-binding protein 2 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	n4bp2	NP_001073436.1
Xenopus tropicalis (tropical clawed frog)	n4bp2	XP_002933515.2

Gene Ontology

The following n4bp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the n4bp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04165	XM_002933469.5 Latest version!	Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OXa41916	XM_018095397.2 Latest version!	Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OXa41916	XM_018095397.1	Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa04165	XM_002933469.4	Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

n4bp2 ( XM_002933469.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933515.2 Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04165
Clone ID Related Accession (Same CDS sequence)	XM_002933469.4 , XM_002933469.5
Accession Version	XM_002933469.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933469.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGAA AAAGAAAAAC TCATTCCTTG AGTCCATTAA GGAAGCCTTC ATCATCTTCC 
TCTACCACTG ATCGTCCATC CTCTGTGGCA TTACCTCTAC CTCCAACCAT GAGTAATAAC 
AACAATGAGG GCCTGCTGGA CAGCATGTGT GACATGTTTC CCGATCTTGA TCCTTCCTTG 
GTTGAAATGG TTCTTTCTGA ATACAAAGAA GTTGAAGTTG TGATGGATTA TCTCCTGGAA 
CTATCAACTT CAGCAAAAGT GGAGACCAAT ACAGAATCGG CCAACGTTTT GGGATTTGAT 
AGTGTAGCAG CTGTTTTGCA CCACAGCAAA AAACCTTTGG TTGGTTCTAA AACTTTGGAA 
GATACTGCTC ATCAGCATTC TGACATTTAT GTAGCTCAGG ATGTATCTCA GGCTCAGGAC 
TGCACTGCTG TGTCTAATAA CTTGGATTTA CTCTTGGATG AGGCCATTAA CAAATATAGT 
CTGTATAGTA GTATAGATCT TGCAGCTGAG CCTGAGCAGT GTCATATACC AGACAACAGT 
AACATTCCAG CTCAAAGTCA GTCTACTTCA GCCATAATCT CCTCTATACT TGCCGATGAA 
GATAATGAAA TGCTAAAAGA CCTTGAAAGT GATGACCAAC AAACTGCCAT AAACCAAACA 
GTTCCATCTA TTAATAAACA ATACACTGCA AGCATCGACC CTAATTTTGC TTTACAGCTT 
CCAAATGAAT CAGGGCTGAA AGACTCTGGG ATGGAATTAA CTGCAAAACT AGATATAGCC 
AAGGCAGATT CCTGCTGCAT TCTAGAGCCT GCATCAGGCT CTTTAGAACG GAATGGGAGG 
AGTAAAACCG CAAGTGAAGA AAAGAGCAAC CATGATGAAA CTGAAGCAGG AATTACACCT 
CTGTCACATG CTAACTCCAT GGGATCTTGG CAAAATAGTC ACCTACAAAA ATCACCCCTT 
TGGAATCCTT TGGCACCTTC ATTTTGCCCA GCAGCAGGTT TACAGCATAG ATTTATAACT 
CCAATACATG TTAGCCGGGC ACCATGGAAT TTCACTGGAG GTGTAAGAAA TGTTAGTAGA 
GATTTCTATT ATAGACCCCC AGTGCCTTCA TTTACATGGA GTGGTAACAA CTTCTCTCCA 
AATGTTTGGC AAACTGCCAG TATAAAATCA GCAGCTCTTT TGGAAGCTGT TCCCCAACCT 
GCTGTTCAAA ATGTGAAAAA AAATACATTT TTTGTGGGAA AAGTTCTCAT TCTTCTTCGA 
GGTGCACCAG GATCAGGAAA AACTACTTTA GCCAGGATGT TGTTACAGCA GAACCCATTG 
GGTATTAACC TAAGTACTGA TGAGTATTTT TACAAAAAGG GGCAATACCG ATATGATGTA 
AATCTGTTAG GGGAAGCACA TGAGTGGAAT CATAAACGAG CCAAAGAAGC CTTTGAGAAG 
AATATTTCCC CAATTATAAT AGATAATACC AACTTGCAAG CTTGGGAAAT GAAGCCATAT 
GTGTCTATGG CCATGAAACA CAAGTACAAA GTCACATTTC GTGAACCTGA TACTTGGTGG 
AAGTATAAAC CAAAAGAACT GGAAAGGCGT AACAGTCATG GAGTAACTAA AGAGAAGATC 
AAGAGAATGT TAGAAAATTT TGAACGCGTG ACCGTGAATT CCATTTTAAA TTTATCACGT 
GTAAAGGGGC CAGAACAAAC TGACCTTGGT GAAATAAAAC AAACTGCTGA CAAAAGAGGA 
CAAAACAGTA ATGCCCCAGC CACTCTGAAG AAAGAAGATA TAAGTTCTAC TTCTGAGGAA 
AAAGTAGATG ATAAACGTAT TGCTGATATG GCTGAAGCAT TTTGTAGCCA TTTGGGAGCT 
GAAACTTCAA AAATGTATCC AGACATTTTA CTTAATTCTA CAGATTCAGT GATGAATATT 
CAGACGTCAG GTCATGGTAA AGAGGAACAG TGGGATAGTG TAGTTTCTGA AGAGGACATA 
TATACCAGCC AGAATGCCGA AACCACTGAT GATGATGCCT TTTTACATCA GATGTCTCCT 
GAAAATGAAA TCATCATTAA AGAAAATGTT TCACCTACAG AGGATGCCTT GGTTTTACCT 
GGGCTAATGC CAGAGTTGCT AAACTTTGTT GGAGACTGGC CAGTTGAGCA CACTATGGGT 
CAGAGAGCCC CACGGAGTAG GAGCAAGATT AGAGCAAATT CTACAAGTAA TGAATCATGT 
TCCCTCCAGC TCAGTGAGTT GACAAATGGT GATTCTAAAG AACTGGATAG TCACACTATA 
ACACATGATT TGTTAAATCT AAGCAAAGAA ATCGATAAAT GTACCATTTT ACAAACAGAT 
TATCTTGGAG ATTTGCCAAA TTTGTGTTTT GGGTGTAACG AAGAAAATAT GCAATTAGAA 
GATCAAGTCT GCTCAGCTTT AGAAAGAGAG GCAGAGACGT ACATACCTCA TACAGTACAG 
AACTCTGAAT CAAATCATGT TGAGAAAGAT CCACAAGCAG TCACTGAAGT TACTAAAGAT 
TTGCAAAGCC TTGATATATC TAATATTAAC GAAAATGAAC TTTCAGAATG CGAAACACCA 
GAGAAAAAAA CAAGGCAATG CAAACGAAAT GTTAGACAGT GTAAACTGGC TTTAACTTTT 
ACAAATAGCT GTTCTGACTC TTCCCAAACA GAAGATATAC TGACATATCA ACCAAATGCT 
CCAGTAAATG AAGTCTACTG TGGACCTAGT AAATGTTCGC AAACTGAGCC TCATGAATTT 
GCTTTAGCCT GGAGGATAGA GAAAAAAAAT ATTGACATCT CCAATTCGGC GAAAGTCCTC 
ACTGGCAAAG GTGAACGTTT TCAGTCAAAA TTAATGGATG AAAGTTCAGT TTCACAGGAA 
ACTATTCCCT ATAGAGCAAT GCATCATAAA AGTACATTTG TGGAAGAAGA TGAAATAGTT 
TGCTTGGATG ATGAACATAA TCTAAATATC CTTGCTGGAC TCTTTAGGTC AGTTTCATTT 
GACGTGCTTA TTGATCTTTT TGAAAGATGC AATAAGGACA TTGTGTGGGC ATCCAACTTA 
CTCTTGGATT CCGGGGAGAA AATCTACAAA GATGAAGACT GCCAGACAGA GGATTATTTG 
AGTGTTGTAG GAGATGCCAC TTTGGGAACT GGTCATCGTC CAGACCAAGT TCCTGTATGC 
CCTGATGATC TTAATGCTCC ATCTTTTGGT AGTAGTATGT CACATACTGA TGCTTGTTCC 
AGCACACCAC TAAATGAAAA AAGAGAGCAT GCTGATAGTC ACACAGTTAT AGAAAATACC 
AACCTAACAT ATTCAGAGGC CCTAAGTTCA GAAGATTCAA CAAATCTAAA TGTGTTCTCT 
GTCCCTGATA TAACAAATGA ACAAACCATA GAACAACATA AAATGGTTAC AAAATCATTG 
AATATTGAGC AGCCAAAAGA TACTGATTTG CAACCAGTGT CTAGGATATG CTTATCTGAT 
ATAGATATGG AGGCTTCTCA GAAAACAGCT GATGATATTG ACACAGGAAT CAATGTTTTA 
GAAACACACA ACATTGATAA AGATGTTTGT GAACTTCCCT TGTACGATAT TAATGTACAG 
GACAAAATAC GTGGATTTGC TCTAGAAGAG GTGGATGATG CAAAAAAGAA AAACTCTGAT 
TCCCTCAAAG ACTCTTTGCA TTTTGATCAT CTGGAATTGT GTTTGCCTCC AGAACTAGCC 
TTTCAGCTCA GTGAATTGTT TGGCCCTGTA GGAATAGCTC CAGGTTCTTT AACTATTGAG 
GATTGTACTG TACACATAGA CTTAAATCTG GCCAAAGCTA TCCATACAAG ATGGAAAGAA 
TCTATTACGG AGAGGCAGAA ACAAGAATCC TTGTCTTACC AGCTGCTTTT TGAAGATTTA 
TCGCCTTCAG AACTGGATAA TCTGATAAAT GAAGAGGAAT CTAACTTGGC AAAACCTGAA 
TATTCCGATA CATCGTCTGA CACATTCCCT TTCATGGATC AGTGGAATGT ACGAACCAAG 
AAAGTGTCAT TACGCGAAAT AATGTCAGAG GAAATTGCAT TACAGGAACA AGAATGTCAG 
AAGAAGTCTA CACTAAGGAC AAAAAACTGT GCTGCAAAAC TGAAGGAAAA ACAACTTTTT 
GAATTGTTTC CTAACATTGA ACAAAGGCTT CTTATGGATA TTTTTAAGGA AAATGATTAT 
TCTTTAGAGA GGACTGCAGA GTTCCTAAGT GTCGTTCTGG AAGCTGGCCC AGTGCAGAAT 
ATTGTTGCCT GTGATTTAAA ACAAACAGAT GCCTCTACCT CTGAAAACCC TAAATCAAAG 
AAATTAAATC TGGACAAAAA AACCATCCCT GAGAATTTCT ATCAGGACTT TGAATGCCCA 
GAGTATGATG ATTTCCGGGC TGAAGCTTTC CTTTACCGTA GAAAGCAACA AGAGTGTTAC 
AGGAAAGCAA GCGAGGCTCA CAGCAGAGGC ATGAAACAAG TTGCAACATA TTATGCACAA 
CAGGGGTACC TCTATGGCCA GAAGATGAAA CAGGAAAACC ACAGAGCTGC TGTACAGATC 
TTTGAAAGAA CTAATGAATT CTTGTTGCCA GAGAATATAT TAGACCTGCA CGGGCTCCAT 
GTTAGTGAGG CAATGAAACA ATTGCGGCAG GTACTTCAGA ACAAGATGGA AGATTATAAA 
AAGAATGGTG GCAAATCTTA CCTTTCCGTG ATAACTGGAA GAGGAAATCA TAGCCAAGGA 
GGAGTCCCTC GCATTAAATT AGCAGTCATT GACTATCTCA CAAACCACAG CTTCAGGTTT 
CAGGAGGTAA GGCCGGGAGT GCTGCATATC ACCCTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933515.2
CDS	166..5007
Translation	MPRKRKTHSLSPLRKPSSSSSTTDRPSSVALPLPPTMSNNNNEGLLDSMCDMFPDLDPSLVEMVLSEYKEVEVVMDYLLELSTSAKVETNTESANVLGFDSVAAVLHHSKKPLVGSKTLEDTAHQHSDIYVAQDVSQAQDCTAVSNNLDLLLDEAINKYSLYSSIDLAAEPEQCHIPDNSNIPAQSQSTSAIISSILADEDNEMLKDLESDDQQTAINQTVPSINKQYTASIDPNFALQLPNESGLKDSGMELTAKLDIAKADSCCILEPASGSLERNGRSKTASEEKSNHDETEAGITPLSHANSMGSWQNSHLQKSPLWNPLAPSFCPAAGLQHRFITPIHVSRAPWNFTGGVRNVSRDFYYRPPVPSFTWSGNNFSPNVWQTASIKSAALLEAVPQPAVQNVKKNTFFVGKVLILLRGAPGSGKTTLARMLLQQNPLGINLSTDEYFYKKGQYRYDVNLLGEAHEWNHKRAKEAFEKNISPIIIDNTNLQAWEMKPYVSMAMKHKYKVTFREPDTWWKYKPKELERRNSHGVTKEKIKRMLENFERVTVNSILNLSRVKGPEQTDLGEIKQTADKRGQNSNAPATLKKEDISSTSEEKVDDKRIADMAEAFCSHLGAETSKMYPDILLNSTDSVMNIQTSGHGKEEQWDSVVSEEDIYTSQNAETTDDDAFLHQMSPENEIIIKENVSPTEDALVLPGLMPELLNFVGDWPVEHTMGQRAPRSRSKIRANSTSNESCSLQLSELTNGDSKELDSHTITHDLLNLSKEIDKCTILQTDYLGDLPNLCFGCNEENMQLEDQVCSALEREAETYIPHTVQNSESNHVEKDPQAVTEVTKDLQSLDISNINENELSECETPEKKTRQCKRNVRQCKLALTFTNSCSDSSQTEDILTYQPNAPVNEVYCGPSKCSQTEPHEFALAWRIEKKNIDISNSAKVLTGKGERFQSKLMDESSVSQETIPYRAMHHKSTFVEEDEIVCLDDEHNLNILAGLFRSVSFDVLIDLFERCNKDIVWASNLLLDSGEKIYKDEDCQTEDYLSVVGDATLGTGHRPDQVPVCPDDLNAPSFGSSMSHTDACSSTPLNEKREHADSHTVIENTNLTYSEALSSEDSTNLNVFSVPDITNEQTIEQHKMVTKSLNIEQPKDTDLQPVSRICLSDIDMEASQKTADDIDTGINVLETHNIDKDVCELPLYDINVQDKIRGFALEEVDDAKKKNSDSLKDSLHFDHLELCLPPELAFQLSELFGPVGIAPGSLTIEDCTVHIDLNLAKAIHTRWKESITERQKQESLSYQLLFEDLSPSELDNLINEEESNLAKPEYSDTSSDTFPFMDQWNVRTKKVSLREIMSEEIALQEQECQKKSTLRTKNCAAKLKEKQLFELFPNIEQRLLMDIFKENDYSLERTAEFLSVVLEAGPVQNIVACDLKQTDASTSENPKSKKLNLDKKTIPENFYQDFECPEYDDFRAEAFLYRRKQQECYRKASEAHSRGMKQVATYYAQQGYLYGQKMKQENHRAAVQIFERTNEFLLPENILDLHGLHVSEAMKQLRQVLQNKMEDYKKNGGKSYLSVITGRGNHSQGGVPRIKLAVIDYLTNHSFRFQEVRPGVLHITLK

Target ORF information:

RefSeq Version	XM_002933469.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933469.5

ORF Insert Sequence:

ATGCCAAGAA AAAGAAAAAC TCATTCCTTG AGTCCATTAA GGAAGCCTTC ATCATCTTCC 
TCTACCACTG ATCGTCCATC CTCTGTGGCA TTACCTCTAC CTCCAACCAT GAGTAATAAC 
AACAATGAGG GCCTGCTGGA CAGCATGTGT GACATGTTTC CCGATCTTGA TCCTTCCTTG 
GTTGAAATGG TTCTTTCTGA ATACAAAGAA GTTGAAGTTG TGATGGATTA TCTCCTGGAA 
CTATCAACTT CAGCAAAAGT GGAGACCAAT ACAGAATCGG CCAACGTTTT GGGATTTGAT 
AGTGTAGCAG CTGTTTTGCA CCACAGCAAA AAACCTTTGG TTGGTTCTAA AACTTTGGAA 
GATACTGCTC ATCAGCATTC TGACATTTAT GTAGCTCAGG ATGTATCTCA GGCTCAGGAC 
TGCACTGCTG TGTCTAATAA CTTGGATTTA CTCTTGGATG AGGCCATTAA CAAATATAGT 
CTGTATAGTA GTATAGATCT TGCAGCTGAG CCTGAGCAGT GTCATATACC AGACAACAGT 
AACATTCCAG CTCAAAGTCA GTCTACTTCA GCCATAATCT CCTCTATACT TGCCGATGAA 
GATAATGAAA TGCTAAAAGA CCTTGAAAGT GATGACCAAC AAACTGCCAT AAACCAAACA 
GTTCCATCTA TTAATAAACA ATACACTGCA AGCATCGACC CTAATTTTGC TTTACAGCTT 
CCAAATGAAT CAGGGCTGAA AGACTCTGGG ATGGAATTAA CTGCAAAACT AGATATAGCC 
AAGGCAGATT CCTGCTGCAT TCTAGAGCCT GCATCAGGCT CTTTAGAACG GAATGGGAGG 
AGTAAAACCG CAAGTGAAGA AAAGAGCAAC CATGATGAAA CTGAAGCAGG AATTACACCT 
CTGTCACATG CTAACTCCAT GGGATCTTGG CAAAATAGTC ACCTACAAAA ATCACCCCTT 
TGGAATCCTT TGGCACCTTC ATTTTGCCCA GCAGCAGGTT TACAGCATAG ATTTATAACT 
CCAATACATG TTAGCCGGGC ACCATGGAAT TTCACTGGAG GTGTAAGAAA TGTTAGTAGA 
GATTTCTATT ATAGACCCCC AGTGCCTTCA TTTACATGGA GTGGTAACAA CTTCTCTCCA 
AATGTTTGGC AAACTGCCAG TATAAAATCA GCAGCTCTTT TGGAAGCTGT TCCCCAACCT 
GCTGTTCAAA ATGTGAAAAA AAATACATTT TTTGTGGGAA AAGTTCTCAT TCTTCTTCGA 
GGTGCACCAG GATCAGGAAA AACTACTTTA GCCAGGATGT TGTTACAGCA GAACCCATTG 
GGTATTAACC TAAGTACTGA TGAGTATTTT TACAAAAAGG GGCAATACCG ATATGATGTA 
AATCTGTTAG GGGAAGCACA TGAGTGGAAT CATAAACGAG CCAAAGAAGC CTTTGAGAAG 
AATATTTCCC CAATTATAAT AGATAATACC AACTTGCAAG CTTGGGAAAT GAAGCCATAT 
GTGTCTATGG CCATGAAACA CAAGTACAAA GTCACATTTC GTGAACCTGA TACTTGGTGG 
AAGTATAAAC CAAAAGAACT GGAAAGGCGT AACAGTCATG GAGTAACTAA AGAGAAGATC 
AAGAGAATGT TAGAAAATTT TGAACGCGTG ACCGTGAATT CCATTTTAAA TTTATCACGT 
GTAAAGGGGC CAGAACAAAC TGACCTTGGT GAAATAAAAC AAACTGCTGA CAAAAGAGGA 
CAAAACAGTA ATGCCCCAGC CACTCTGAAG AAAGAAGATA TAAGTTCTAC TTCTGAGGAA 
AAAGTAGATG ATAAACGTAT TGCTGATATG GCTGAAGCAT TTTGTAGCCA TTTGGGAGCT 
GAAACTTCAA AAATGTATCC AGACATTTTA CTTAATTCTA CAGATTCAGT GATGAATATT 
CAGACGTCAG GTCATGGTAA AGAGGAACAG TGGGATAGTG TAGTTTCTGA AGAGGACATA 
TATACCAGCC AGAATGCCGA AACCACTGAT GATGATGCCT TTTTACATCA GATGTCTCCT 
GAAAATGAAA TCATCATTAA AGAAAATGTT TCACCTACAG AGGATGCCTT GGTTTTACCT 
GGGCTAATGC CAGAGTTGCT AAACTTTGTT GGAGACTGGC CAGTTGAGCA CACTATGGGT 
CAGAGAGCCC CACGGAGTAG GAGCAAGATT AGAGCAAATT CTACAAGTAA TGAATCATGT 
TCCCTCCAGC TCAGTGAGTT GACAAATGGT GATTCTAAAG AACTGGATAG TCACACTATA 
ACACATGATT TGTTAAATCT AAGCAAAGAA ATCGATAAAT GTACCATTTT ACAAACAGAT 
TATCTTGGAG ATTTGCCAAA TTTGTGTTTT GGGTGTAACG AAGAAAATAT GCAATTAGAA 
GATCAAGTCT GCTCAGCTTT AGAAAGAGAG GCAGAGACGT ACATACCTCA TACAGTACAG 
AACTCTGAAT CAAATCATGT TGAGAAAGAT CCACAAGCAG TCACTGAAGT TACTAAAGAT 
TTGCAAAGCC TTGATATATC TAATATTAAC GAAAATGAAC TTTCAGAATG CGAAACACCA 
GAGAAAAAAA CAAGGCAATG CAAACGAAAT GTTAGACAGT GTAAACTGGC TTTAACTTTT 
ACAAATAGCT GTTCTGACTC TTCCCAAACA GAAGATATAC TGACATATCA ACCAAATGCT 
CCAGTAAATG AAGTCTACTG TGGACCTAGT AAATGTTCGC AAACTGAGCC TCATGAATTT 
GCTTTAGCCT GGAGGATAGA GAAAAAAAAT ATTGACATCT CCAATTCGGC GAAAGTCCTC 
ACTGGCAAAG GTGAACGTTT TCAGTCAAAA TTAATGGATG AAAGTTCAGT TTCACAGGAA 
ACTATTCCCT ATAGAGCAAT GCATCATAAA AGTACATTTG TGGAAGAAGA TGAAATAGTT 
TGCTTGGATG ATGAACATAA TCTAAATATC CTTGCTGGAC TCTTTAGGTC AGTTTCATTT 
GACGTGCTTA TTGATCTTTT TGAAAGATGC AATAAGGACA TTGTGTGGGC ATCCAACTTA 
CTCTTGGATT CCGGGGAGAA AATCTACAAA GATGAAGACT GCCAGACAGA GGATTATTTG 
AGTGTTGTAG GAGATGCCAC TTTGGGAACT GGTCATCGTC CAGACCAAGT TCCTGTATGC 
CCTGATGATC TTAATGCTCC ATCTTTTGGT AGTAGTATGT CACATACTGA TGCTTGTTCC 
AGCACACCAC TAAATGAAAA AAGAGAGCAT GCTGATAGTC ACACAGTTAT AGAAAATACC 
AACCTAACAT ATTCAGAGGC CCTAAGTTCA GAAGATTCAA CAAATCTAAA TGTGTTCTCT 
GTCCCTGATA TAACAAATGA ACAAACCATA GAACAACATA AAATGGTTAC AAAATCATTG 
AATATTGAGC AGCCAAAAGA TACTGATTTG CAACCAGTGT CTAGGATATG CTTATCTGAT 
ATAGATATGG AGGCTTCTCA GAAAACAGCT GATGATATTG ACACAGGAAT CAATGTTTTA 
GAAACACACA ACATTGATAA AGATGTTTGT GAACTTCCCT TGTACGATAT TAATGTACAG 
GACAAAATAC GTGGATTTGC TCTAGAAGAG GTGGATGATG CAAAAAAGAA AAACTCTGAT 
TCCCTCAAAG ACTCTTTGCA TTTTGATCAT CTGGAATTGT GTTTGCCTCC AGAACTAGCC 
TTTCAGCTCA GTGAATTGTT TGGCCCTGTA GGAATAGCTC CAGGTTCTTT AACTATTGAG 
GATTGTACTG TACACATAGA CTTAAATCTG GCCAAAGCTA TCCATACAAG ATGGAAAGAA 
TCTATTACGG AGAGGCAGAA ACAAGAATCC TTGTCTTACC AGCTGCTTTT TGAAGATTTA 
TCGCCTTCAG AACTGGATAA TCTGATAAAT GAAGAGGAAT CTAACTTGGC AAAACCTGAA 
TATTCCGATA CATCGTCTGA CACATTCCCT TTCATGGATC AGTGGAATGT ACGAACCAAG 
AAAGTGTCAT TACGCGAAAT AATGTCAGAG GAAATTGCAT TACAGGAACA AGAATGTCAG 
AAGAAGTCTA CACTAAGGAC AAAAAACTGT GCTGCAAAAC TGAAGGAAAA ACAACTTTTT 
GAATTGTTTC CTAACATTGA ACAAAGGCTT CTTATGGATA TTTTTAAGGA AAATGATTAT 
TCTTTAGAGA GGACTGCAGA GTTCCTAAGT GTCGTTCTGG AAGCTGGCCC AGTGCAGAAT 
ATTGTTGCCT GTGATTTAAA ACAAACAGAT GCCTCTACCT CTGAAAACCC TAAATCAAAG 
AAATTAAATC TGGACAAAAA AACCATCCCT GAGAATTTCT ATCAGGACTT TGAATGCCCA 
GAGTATGATG ATTTCCGGGC TGAAGCTTTC CTTTACCGTA GAAAGCAACA AGAGTGTTAC 
AGGAAAGCAA GCGAGGCTCA CAGCAGAGGC ATGAAACAAG TTGCAACATA TTATGCACAA 
CAGGGGTACC TCTATGGCCA GAAGATGAAA CAGGAAAACC ACAGAGCTGC TGTACAGATC 
TTTGAAAGAA CTAATGAATT CTTGTTGCCA GAGAATATAT TAGACCTGCA CGGGCTCCAT 
GTTAGTGAGG CAATGAAACA ATTGCGGCAG GTACTTCAGA ACAAGATGGA AGATTATAAA 
AAGAATGGTG GCAAATCTTA CCTTTCCGTG ATAACTGGAA GAGGAAATCA TAGCCAAGGA 
GGAGTCCCTC GCATTAAATT AGCAGTCATT GACTATCTCA CAAACCACAG CTTCAGGTTT 
CAGGAGGTAA GGCCGGGAGT GCTGCATATC ACCCTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

n4bp2 ( XM_018095397.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950886.1 Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa41916
Clone ID Related Accession (Same CDS sequence)	XM_018095397.1 , XM_018095397.2
Accession Version	XM_018095397.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGAA AAAGAAAAAC TCATTCCTTG AGTCCATTAA GGAAGCCTTC ATCATCTTCC 
TCTACCACTG ATCGTCCATC CTCTGTGGCA TTACCTCTAC CTCCAACCAT GAGTAATAAC 
AACAATGAGG GCCTGCTGGA CAGCATGTGT GACATGTTTC CCGATCTTGA TCCTTCCTTG 
GTTGAAATGG TTCTTTCTGA ATACAAAGAA GTTGAAGTTG TGATGGATTA TCTCCTGGAA 
CTATCAACTT CAGCAAAAGT GGAGACCAAT ACAGAATCGG CCAACGTTTT GGGATTTGAT 
AGTGTAGCAG CTGTTTTGCA CCACAGCAAA AAACCTTTGG TTGGTTCTAA AACTTTGGAA 
GATACTGCTC ATCAGCATTC TGACATTTAT GTAGCTCAGG ATGTATCTCA GGCTCAGGAC 
TGCACTGCTG TGTCTAATAA CTTGGATTTA CTCTTGGATG AGGCCATTAA CAAATATAGT 
CTGTATAGTA GTATAGATCT TGCAGCTGAG CCTGAGCAGT GTCATATACC AGACAACAGT 
AACATTCCAG CTCAAAGTCA GTCTACTTCA GCCATAATCT CCTCTATACT TGCCGATGAA 
GATAATGAAA TGCTAAAAGA CCTTGAAAGT GATGACCAAC AAACTGCCAT AAACCAAACA 
GTTCCATCTA TTAATAAACA ATACACTGCA AGCATCGACC CTAATTTTGC TTTACAGCTT 
CCAAATGAAT CAGGGCTGAA AGACTCTGGG ATGGAATTAA CTGCAAAACT AGATATAGCC 
AAGGCAGATT CCTGCTGCAT TCTAGAGCCT GCATCAGGCT CTTTAGAACG GAATGGGAGG 
AGTAAAACCG CAAGTGAAGA AAAGAGCAAC CATGATGAAA CTGAAGCAGG AATTACACCT 
CTGTCACATG CTAACTCCAT GGGATCTTGG CAAAATAGTC ACCTACAAAA ATCACCCCTT 
TGGAATCCTT TGGCACCTTC ATTTTGCCCA GCAGCAGGTT TACAGCATAG ATTTATAACT 
CCAATACATG TTAGCCGGGC ACCATGGAAT TTCACTGGAG GTGTAAGAAA TGTTAGTAGA 
GATTTCTATT ATAGACCCCC AGTGCCTTCA TTTACATGGA GTGGTAACAA CTTCTCTCCA 
AATGTTTGGC AAACTGCCAG TATAAAATCA GCAGCTCTTT TGGAAGCTGT TCCCCAACCT 
GCTGTTCAAA ATGTGAAAAA AAATACATTT TTTGTGGGAA AAGTTCTCAT TCTTCTTCGA 
GGTGCACCAG GATCAGGAAA AACTACTTTA GCCAGGATGT TGTTACAGCA GAACCCATTG 
GGTATTAACC TAAGTACTGA TGAGTATTTT TACAAAAAGG GGCAATACCG ATATGATGTA 
AATCTGTTAG GGGAAGCACA TGAGTGGAAT CATAAACGAG CCAAAGAAGC CTTTGAGAAG 
AATATTTCCC CAATTATAAT AGATAATACC AACTTGCAAG CTTGGGAAAT GAAGCCATAT 
GTGTCTATGG CCATGAAACA CAAGTACAAA GTCACATTTC GTGAACCTGA TACTTGGTGG 
AAGTATAAAC CAAAAGAACT GGAAAGGCGT AACAGTCATG GAGTAACTAA AGAGAAGATC 
AAGAGAATGT TAGAAAATTT TGAACGCGTG ACCGTGAATT CCATTTTAAA TTTATCACGT 
GTAAAGGGGC CAGAACAAAC TGACCTTGGT GAAATAAAAC AAACTGCTGA CAAAAGAGGA 
CAAAACAGTA ATGCCCCAGC CACTCTGAAG AAAGAAGATA TAAGTTCTAC TTCTGAGGAA 
AAAGTAGATG ATAAACGTAT TGCTGATATG GCTGAAGCAT TTTGTAGCCA TTTGGGAGCT 
GAAACTTCAA AAATGTATCC AGACATTTTA CTTAATTCTA CAGATTCAGT GATGAATATT 
CAGACGTCAG GTCATGGTAA AGAGGAACAG TGGGATAGTG TAGTTTCTGA AGAGGACATA 
TATACCAGCC AGAATGCCGA AACCACTGAT GATGATGCCT TTTTACATCA GATGTCTCCT 
GAAAATGAAA TCATCATTAA AGAAAATGTT TCACCTACAG AGGATGCCTT GGTTTTACCT 
GGGCTAATGC CAGAGTTGCT AAACTTTGTT GGAGACTGGC CAGTTGAGCA CACTATGGGT 
CAGAGAGCCC CACGGAGTAG GAGCAAGATT AGAGCAAATT CTACAAGTAA TGAATCATGT 
TCCCTCCAGC TCAGTGAGTT GACAAATGGT GATTCTAAAG AACTGGATAG TCACACTATA 
ACACATGATT TGTTAAATCT AAGCAAAGAA ATCGATAAAT GTACCATTTT ACAAACAGAT 
TATCTTGGAG ATTTGCCAAA TTTGTGTTTT GGGTGTAACG AAGAAAATAT GCAATTAGAA 
GATCAAGTCT GCTCAGCTTT AGAAAGAGAG GCAGAGACGT ACATACCTCA TACAGTACAG 
AACTCTGAAT CAAATCATGT TGAGAAAGAT CCACAAGCAG TCACTGAAGT TACTAAAGAT 
TTGCAAAGCC TTGATATATC TAATATTAAC GAAAATGAAC TTTCAGAATG CGAAACACCA 
GAGAAAAAAA CAAGGCAATG CAAACGAAAT GTTAGACAGT GTAAACTGGC TTTAACTTTT 
ACAAATAGCT GTTCTGACTC TTCCCAAACA GAAGATATAC TGACATATCA ACCAAATGCT 
CCAGTAAATG AAGTCTACTG TGGACCTAGT AAATGTTCGC AAACTGAGCC TCATGAATTT 
GCTTTAGCCT GGAGGATAGA GAAAAAAAAT ATTGACATCT CCAATTCGGC GAAAGTCCTC 
ACTGGCAAAG GTGAACGTTT TCAGTCAAAA TTAATGGATG AAAGTTCAGT TTCACAGGAA 
ACTATTCCCT ATAGAGCAAT GCATCATAAA AGTACATTTG TGGAAGAAGA TGAAATAGTT 
TGCTTGGATG ATGAACATAA TCTAAATATC CTTGCTGGAC TCTTTAGGTC AGTTTCATTT 
GACGTGCTTA TTGATCTTTT TGAAAGATGC AATAAGGACA TTGTGTGGGC ATCCAACTTA 
CTCTTGGATT CCGGGGAGAA AATCTACAAA GATGAAGACT GCCAGACAGA GGATTATTTG 
AGTGTTGTAG GAGATGCCAC TTTGGGAACT GGTCATCGTC CAGACCAAGT TCCTGTATGC 
CCTGATGATC TTAATGCTCC ATCTTTTGGT AGTAGTATGT CACATACTGA TGCTTGTTCC 
AGCACACCAC TAAATGAAAA AAGAGAGCAT GCTGATAGTC ACACAGTTAT AGAAAATACC 
AACCTAACAT ATTCAGAGGC CCTAAGTTCA GAAGATTCAA CAAATCTAAA TGTGTTCTCT 
GTCCCTGATA TAACAAATGA ACAAACCATA GAACAACATA AAATGGTTAC AAAATCATTG 
AATATTGAGC AGCCAAAAGA TACTGATTTG CAACCAGTGT CTAGGATATG CTTATCTGAT 
ATAGATATGG AGGCTTCTCA GAAAACAGCT GATGATATTG ACACAGGAAT CAATGTTTTA 
GAAACACACA ACATTGATAA AGATGTTTGT GAACTTCCCT TGTACGATAT TAATGTACAG 
GACAAAATAC GTGGATTTGC TCTAGAAGAG GTGGATGATG CAAAAAAGAA AAACTCTGAT 
TCCCTCAAAG ACTCTTTGCA TTTTGATCAT CTGGAATTGT GTTTGCCTCC AGAACTAGCC 
TTTCAGCTCA GTGAATTGTT TGGCCCTGTA GGAATAGCTC CAGGTTCTTT AACTATTGAG 
GATTGTACTG TACACATAGA CTTAAATCTG GCCAAAGCTA TCCATACAAG ATGGAAAGAA 
TCTATTACGG AGAGGCAGAA ACAAGAATCC TTGTCTTACC AGCTGCTTTT TGAAGATTTA 
TCGCCTTCAG AACTGGATAA TCTGATAAAT GAAGAGGAAT CTAACTTGGC AAAACCTGAA 
TATTCCGATA CATCGTCTGA CACATTCCCT TTCATGGATC AGTGGAATGT ACGAACCAAG 
AAAGTGTCAT TACGCGAAAT AATGTCAGAG GAAATTGCAT TACAGGAACA AGAATGTCAG 
AAGTCTACAC TAAGGACAAA AAACTGTGCT GCAAAACTGA AGGAAAAACA ACTTTTTGAA 
TTGTTTCCTA ACATTGAACA AAGGCTTCTT ATGGATATTT TTAAGGAAAA TGATTATTCT 
TTAGAGAGGA CTGCAGAGTT CCTAAGTGTC GTTCTGGAAG CTGGCCCAGT GCAGAATATT 
GTTGCCTGTG ATTTAAAACA AACAGATGCC TCTACCTCTG AAAACCCTAA ATCAAAGAAA 
TTAAATCTGG ACAAAAAAAC CATCCCTGAG AATTTCTATC AGGACTTTGA ATGCCCAGAG 
TATGATGATT TCCGGGCTGA AGCTTTCCTT TACCGTAGAA AGCAACAAGA GTGTTACAGG 
AAAGCAAGCG AGGCTCACAG CAGAGGCATG AAACAAGTTG CAACATATTA TGCACAACAG 
GGGTACCTCT ATGGCCAGAA GATGAAACAG GAAAACCACA GAGCTGCTGT ACAGATCTTT 
GAAAGAACTA ATGAATTCTT GTTGCCAGAG AATATATTAG ACCTGCACGG GCTCCATGTT 
AGTGAGGCAA TGAAACAATT GCGGCAGGTA CTTCAGAACA AGATGGAAGA TTATAAAAAG 
AATGGTGGCA AATCTTACCT TTCCGTGATA ACTGGAAGAG GAAATCATAG CCAAGGAGGA 
GTCCCTCGCA TTAAATTAGC AGTCATTGAC TATCTCACAA ACCACAGCTT CAGGTTTCAG 
GAGGTAAGGC CGGGAGTGCT GCATATCACC CTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950886.1
CDS	823..5661
Translation	MPRKRKTHSLSPLRKPSSSSSTTDRPSSVALPLPPTMSNNNNEGLLDSMCDMFPDLDPSLVEMVLSEYKEVEVVMDYLLELSTSAKVETNTESANVLGFDSVAAVLHHSKKPLVGSKTLEDTAHQHSDIYVAQDVSQAQDCTAVSNNLDLLLDEAINKYSLYSSIDLAAEPEQCHIPDNSNIPAQSQSTSAIISSILADEDNEMLKDLESDDQQTAINQTVPSINKQYTASIDPNFALQLPNESGLKDSGMELTAKLDIAKADSCCILEPASGSLERNGRSKTASEEKSNHDETEAGITPLSHANSMGSWQNSHLQKSPLWNPLAPSFCPAAGLQHRFITPIHVSRAPWNFTGGVRNVSRDFYYRPPVPSFTWSGNNFSPNVWQTASIKSAALLEAVPQPAVQNVKKNTFFVGKVLILLRGAPGSGKTTLARMLLQQNPLGINLSTDEYFYKKGQYRYDVNLLGEAHEWNHKRAKEAFEKNISPIIIDNTNLQAWEMKPYVSMAMKHKYKVTFREPDTWWKYKPKELERRNSHGVTKEKIKRMLENFERVTVNSILNLSRVKGPEQTDLGEIKQTADKRGQNSNAPATLKKEDISSTSEEKVDDKRIADMAEAFCSHLGAETSKMYPDILLNSTDSVMNIQTSGHGKEEQWDSVVSEEDIYTSQNAETTDDDAFLHQMSPENEIIIKENVSPTEDALVLPGLMPELLNFVGDWPVEHTMGQRAPRSRSKIRANSTSNESCSLQLSELTNGDSKELDSHTITHDLLNLSKEIDKCTILQTDYLGDLPNLCFGCNEENMQLEDQVCSALEREAETYIPHTVQNSESNHVEKDPQAVTEVTKDLQSLDISNINENELSECETPEKKTRQCKRNVRQCKLALTFTNSCSDSSQTEDILTYQPNAPVNEVYCGPSKCSQTEPHEFALAWRIEKKNIDISNSAKVLTGKGERFQSKLMDESSVSQETIPYRAMHHKSTFVEEDEIVCLDDEHNLNILAGLFRSVSFDVLIDLFERCNKDIVWASNLLLDSGEKIYKDEDCQTEDYLSVVGDATLGTGHRPDQVPVCPDDLNAPSFGSSMSHTDACSSTPLNEKREHADSHTVIENTNLTYSEALSSEDSTNLNVFSVPDITNEQTIEQHKMVTKSLNIEQPKDTDLQPVSRICLSDIDMEASQKTADDIDTGINVLETHNIDKDVCELPLYDINVQDKIRGFALEEVDDAKKKNSDSLKDSLHFDHLELCLPPELAFQLSELFGPVGIAPGSLTIEDCTVHIDLNLAKAIHTRWKESITERQKQESLSYQLLFEDLSPSELDNLINEEESNLAKPEYSDTSSDTFPFMDQWNVRTKKVSLREIMSEEIALQEQECQKSTLRTKNCAAKLKEKQLFELFPNIEQRLLMDIFKENDYSLERTAEFLSVVLEAGPVQNIVACDLKQTDASTSENPKSKKLNLDKKTIPENFYQDFECPEYDDFRAEAFLYRRKQQECYRKASEAHSRGMKQVATYYAQQGYLYGQKMKQENHRAAVQIFERTNEFLLPENILDLHGLHVSEAMKQLRQVLQNKMEDYKKNGGKSYLSVITGRGNHSQGGVPRIKLAVIDYLTNHSFRFQEVRPGVLHITLK

Target ORF information:

RefSeq Version	XM_018095397.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095397.1

ORF Insert Sequence:

ATGCCAAGAA AAAGAAAAAC TCATTCCTTG AGTCCATTAA GGAAGCCTTC ATCATCTTCC 
TCTACCACTG ATCGTCCATC CTCTGTGGCA TTACCTCTAC CTCCAACCAT GAGTAATAAC 
AACAATGAGG GCCTGCTGGA CAGCATGTGT GACATGTTTC CCGATCTTGA TCCTTCCTTG 
GTTGAAATGG TTCTTTCTGA ATACAAAGAA GTTGAAGTTG TGATGGATTA TCTCCTGGAA 
CTATCAACTT CAGCAAAAGT GGAGACCAAT ACAGAATCGG CCAACGTTTT GGGATTTGAT 
AGTGTAGCAG CTGTTTTGCA CCACAGCAAA AAACCTTTGG TTGGTTCTAA AACTTTGGAA 
GATACTGCTC ATCAGCATTC TGACATTTAT GTAGCTCAGG ATGTATCTCA GGCTCAGGAC 
TGCACTGCTG TGTCTAATAA CTTGGATTTA CTCTTGGATG AGGCCATTAA CAAATATAGT 
CTGTATAGTA GTATAGATCT TGCAGCTGAG CCTGAGCAGT GTCATATACC AGACAACAGT 
AACATTCCAG CTCAAAGTCA GTCTACTTCA GCCATAATCT CCTCTATACT TGCCGATGAA 
GATAATGAAA TGCTAAAAGA CCTTGAAAGT GATGACCAAC AAACTGCCAT AAACCAAACA 
GTTCCATCTA TTAATAAACA ATACACTGCA AGCATCGACC CTAATTTTGC TTTACAGCTT 
CCAAATGAAT CAGGGCTGAA AGACTCTGGG ATGGAATTAA CTGCAAAACT AGATATAGCC 
AAGGCAGATT CCTGCTGCAT TCTAGAGCCT GCATCAGGCT CTTTAGAACG GAATGGGAGG 
AGTAAAACCG CAAGTGAAGA AAAGAGCAAC CATGATGAAA CTGAAGCAGG AATTACACCT 
CTGTCACATG CTAACTCCAT GGGATCTTGG CAAAATAGTC ACCTACAAAA ATCACCCCTT 
TGGAATCCTT TGGCACCTTC ATTTTGCCCA GCAGCAGGTT TACAGCATAG ATTTATAACT 
CCAATACATG TTAGCCGGGC ACCATGGAAT TTCACTGGAG GTGTAAGAAA TGTTAGTAGA 
GATTTCTATT ATAGACCCCC AGTGCCTTCA TTTACATGGA GTGGTAACAA CTTCTCTCCA 
AATGTTTGGC AAACTGCCAG TATAAAATCA GCAGCTCTTT TGGAAGCTGT TCCCCAACCT 
GCTGTTCAAA ATGTGAAAAA AAATACATTT TTTGTGGGAA AAGTTCTCAT TCTTCTTCGA 
GGTGCACCAG GATCAGGAAA AACTACTTTA GCCAGGATGT TGTTACAGCA GAACCCATTG 
GGTATTAACC TAAGTACTGA TGAGTATTTT TACAAAAAGG GGCAATACCG ATATGATGTA 
AATCTGTTAG GGGAAGCACA TGAGTGGAAT CATAAACGAG CCAAAGAAGC CTTTGAGAAG 
AATATTTCCC CAATTATAAT AGATAATACC AACTTGCAAG CTTGGGAAAT GAAGCCATAT 
GTGTCTATGG CCATGAAACA CAAGTACAAA GTCACATTTC GTGAACCTGA TACTTGGTGG 
AAGTATAAAC CAAAAGAACT GGAAAGGCGT AACAGTCATG GAGTAACTAA AGAGAAGATC 
AAGAGAATGT TAGAAAATTT TGAACGCGTG ACCGTGAATT CCATTTTAAA TTTATCACGT 
GTAAAGGGGC CAGAACAAAC TGACCTTGGT GAAATAAAAC AAACTGCTGA CAAAAGAGGA 
CAAAACAGTA ATGCCCCAGC CACTCTGAAG AAAGAAGATA TAAGTTCTAC TTCTGAGGAA 
AAAGTAGATG ATAAACGTAT TGCTGATATG GCTGAAGCAT TTTGTAGCCA TTTGGGAGCT 
GAAACTTCAA AAATGTATCC AGACATTTTA CTTAATTCTA CAGATTCAGT GATGAATATT 
CAGACGTCAG GTCATGGTAA AGAGGAACAG TGGGATAGTG TAGTTTCTGA AGAGGACATA 
TATACCAGCC AGAATGCCGA AACCACTGAT GATGATGCCT TTTTACATCA GATGTCTCCT 
GAAAATGAAA TCATCATTAA AGAAAATGTT TCACCTACAG AGGATGCCTT GGTTTTACCT 
GGGCTAATGC CAGAGTTGCT AAACTTTGTT GGAGACTGGC CAGTTGAGCA CACTATGGGT 
CAGAGAGCCC CACGGAGTAG GAGCAAGATT AGAGCAAATT CTACAAGTAA TGAATCATGT 
TCCCTCCAGC TCAGTGAGTT GACAAATGGT GATTCTAAAG AACTGGATAG TCACACTATA 
ACACATGATT TGTTAAATCT AAGCAAAGAA ATCGATAAAT GTACCATTTT ACAAACAGAT 
TATCTTGGAG ATTTGCCAAA TTTGTGTTTT GGGTGTAACG AAGAAAATAT GCAATTAGAA 
GATCAAGTCT GCTCAGCTTT AGAAAGAGAG GCAGAGACGT ACATACCTCA TACAGTACAG 
AACTCTGAAT CAAATCATGT TGAGAAAGAT CCACAAGCAG TCACTGAAGT TACTAAAGAT 
TTGCAAAGCC TTGATATATC TAATATTAAC GAAAATGAAC TTTCAGAATG CGAAACACCA 
GAGAAAAAAA CAAGGCAATG CAAACGAAAT GTTAGACAGT GTAAACTGGC TTTAACTTTT 
ACAAATAGCT GTTCTGACTC TTCCCAAACA GAAGATATAC TGACATATCA ACCAAATGCT 
CCAGTAAATG AAGTCTACTG TGGACCTAGT AAATGTTCGC AAACTGAGCC TCATGAATTT 
GCTTTAGCCT GGAGGATAGA GAAAAAAAAT ATTGACATCT CCAATTCGGC GAAAGTCCTC 
ACTGGCAAAG GTGAACGTTT TCAGTCAAAA TTAATGGATG AAAGTTCAGT TTCACAGGAA 
ACTATTCCCT ATAGAGCAAT GCATCATAAA AGTACATTTG TGGAAGAAGA TGAAATAGTT 
TGCTTGGATG ATGAACATAA TCTAAATATC CTTGCTGGAC TCTTTAGGTC AGTTTCATTT 
GACGTGCTTA TTGATCTTTT TGAAAGATGC AATAAGGACA TTGTGTGGGC ATCCAACTTA 
CTCTTGGATT CCGGGGAGAA AATCTACAAA GATGAAGACT GCCAGACAGA GGATTATTTG 
AGTGTTGTAG GAGATGCCAC TTTGGGAACT GGTCATCGTC CAGACCAAGT TCCTGTATGC 
CCTGATGATC TTAATGCTCC ATCTTTTGGT AGTAGTATGT CACATACTGA TGCTTGTTCC 
AGCACACCAC TAAATGAAAA AAGAGAGCAT GCTGATAGTC ACACAGTTAT AGAAAATACC 
AACCTAACAT ATTCAGAGGC CCTAAGTTCA GAAGATTCAA CAAATCTAAA TGTGTTCTCT 
GTCCCTGATA TAACAAATGA ACAAACCATA GAACAACATA AAATGGTTAC AAAATCATTG 
AATATTGAGC AGCCAAAAGA TACTGATTTG CAACCAGTGT CTAGGATATG CTTATCTGAT 
ATAGATATGG AGGCTTCTCA GAAAACAGCT GATGATATTG ACACAGGAAT CAATGTTTTA 
GAAACACACA ACATTGATAA AGATGTTTGT GAACTTCCCT TGTACGATAT TAATGTACAG 
GACAAAATAC GTGGATTTGC TCTAGAAGAG GTGGATGATG CAAAAAAGAA AAACTCTGAT 
TCCCTCAAAG ACTCTTTGCA TTTTGATCAT CTGGAATTGT GTTTGCCTCC AGAACTAGCC 
TTTCAGCTCA GTGAATTGTT TGGCCCTGTA GGAATAGCTC CAGGTTCTTT AACTATTGAG 
GATTGTACTG TACACATAGA CTTAAATCTG GCCAAAGCTA TCCATACAAG ATGGAAAGAA 
TCTATTACGG AGAGGCAGAA ACAAGAATCC TTGTCTTACC AGCTGCTTTT TGAAGATTTA 
TCGCCTTCAG AACTGGATAA TCTGATAAAT GAAGAGGAAT CTAACTTGGC AAAACCTGAA 
TATTCCGATA CATCGTCTGA CACATTCCCT TTCATGGATC AGTGGAATGT ACGAACCAAG 
AAAGTGTCAT TACGCGAAAT AATGTCAGAG GAAATTGCAT TACAGGAACA AGAATGTCAG 
AAGTCTACAC TAAGGACAAA AAACTGTGCT GCAAAACTGA AGGAAAAACA ACTTTTTGAA 
TTGTTTCCTA ACATTGAACA AAGGCTTCTT ATGGATATTT TTAAGGAAAA TGATTATTCT 
TTAGAGAGGA CTGCAGAGTT CCTAAGTGTC GTTCTGGAAG CTGGCCCAGT GCAGAATATT 
GTTGCCTGTG ATTTAAAACA AACAGATGCC TCTACCTCTG AAAACCCTAA ATCAAAGAAA 
TTAAATCTGG ACAAAAAAAC CATCCCTGAG AATTTCTATC AGGACTTTGA ATGCCCAGAG 
TATGATGATT TCCGGGCTGA AGCTTTCCTT TACCGTAGAA AGCAACAAGA GTGTTACAGG 
AAAGCAAGCG AGGCTCACAG CAGAGGCATG AAACAAGTTG CAACATATTA TGCACAACAG 
GGGTACCTCT ATGGCCAGAA GATGAAACAG GAAAACCACA GAGCTGCTGT ACAGATCTTT 
GAAAGAACTA ATGAATTCTT GTTGCCAGAG AATATATTAG ACCTGCACGG GCTCCATGTT 
AGTGAGGCAA TGAAACAATT GCGGCAGGTA CTTCAGAACA AGATGGAAGA TTATAAAAAG 
AATGGTGGCA AATCTTACCT TTCCGTGATA ACTGGAAGAG GAAATCATAG CCAAGGAGGA 
GTCCCTCGCA TTAAATTAGC AGTCATTGAC TATCTCACAA ACCACAGCTT CAGGTTTCAG 
GAGGTAAGGC CGGGAGTGCT GCATATCACC CTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

n4bp2 ( XM_018095397.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950886.1 Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X2, mRNA.

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CloneID	OXa41916
Clone ID Related Accession (Same CDS sequence)	XM_018095397.1 , XM_018095397.2
Accession Version	XM_018095397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGAA AAAGAAAAAC TCATTCCTTG AGTCCATTAA GGAAGCCTTC ATCATCTTCC 
TCTACCACTG ATCGTCCATC CTCTGTGGCA TTACCTCTAC CTCCAACCAT GAGTAATAAC 
AACAATGAGG GCCTGCTGGA CAGCATGTGT GACATGTTTC CCGATCTTGA TCCTTCCTTG 
GTTGAAATGG TTCTTTCTGA ATACAAAGAA GTTGAAGTTG TGATGGATTA TCTCCTGGAA 
CTATCAACTT CAGCAAAAGT GGAGACCAAT ACAGAATCGG CCAACGTTTT GGGATTTGAT 
AGTGTAGCAG CTGTTTTGCA CCACAGCAAA AAACCTTTGG TTGGTTCTAA AACTTTGGAA 
GATACTGCTC ATCAGCATTC TGACATTTAT GTAGCTCAGG ATGTATCTCA GGCTCAGGAC 
TGCACTGCTG TGTCTAATAA CTTGGATTTA CTCTTGGATG AGGCCATTAA CAAATATAGT 
CTGTATAGTA GTATAGATCT TGCAGCTGAG CCTGAGCAGT GTCATATACC AGACAACAGT 
AACATTCCAG CTCAAAGTCA GTCTACTTCA GCCATAATCT CCTCTATACT TGCCGATGAA 
GATAATGAAA TGCTAAAAGA CCTTGAAAGT GATGACCAAC AAACTGCCAT AAACCAAACA 
GTTCCATCTA TTAATAAACA ATACACTGCA AGCATCGACC CTAATTTTGC TTTACAGCTT 
CCAAATGAAT CAGGGCTGAA AGACTCTGGG ATGGAATTAA CTGCAAAACT AGATATAGCC 
AAGGCAGATT CCTGCTGCAT TCTAGAGCCT GCATCAGGCT CTTTAGAACG GAATGGGAGG 
AGTAAAACCG CAAGTGAAGA AAAGAGCAAC CATGATGAAA CTGAAGCAGG AATTACACCT 
CTGTCACATG CTAACTCCAT GGGATCTTGG CAAAATAGTC ACCTACAAAA ATCACCCCTT 
TGGAATCCTT TGGCACCTTC ATTTTGCCCA GCAGCAGGTT TACAGCATAG ATTTATAACT 
CCAATACATG TTAGCCGGGC ACCATGGAAT TTCACTGGAG GTGTAAGAAA TGTTAGTAGA 
GATTTCTATT ATAGACCCCC AGTGCCTTCA TTTACATGGA GTGGTAACAA CTTCTCTCCA 
AATGTTTGGC AAACTGCCAG TATAAAATCA GCAGCTCTTT TGGAAGCTGT TCCCCAACCT 
GCTGTTCAAA ATGTGAAAAA AAATACATTT TTTGTGGGAA AAGTTCTCAT TCTTCTTCGA 
GGTGCACCAG GATCAGGAAA AACTACTTTA GCCAGGATGT TGTTACAGCA GAACCCATTG 
GGTATTAACC TAAGTACTGA TGAGTATTTT TACAAAAAGG GGCAATACCG ATATGATGTA 
AATCTGTTAG GGGAAGCACA TGAGTGGAAT CATAAACGAG CCAAAGAAGC CTTTGAGAAG 
AATATTTCCC CAATTATAAT AGATAATACC AACTTGCAAG CTTGGGAAAT GAAGCCATAT 
GTGTCTATGG CCATGAAACA CAAGTACAAA GTCACATTTC GTGAACCTGA TACTTGGTGG 
AAGTATAAAC CAAAAGAACT GGAAAGGCGT AACAGTCATG GAGTAACTAA AGAGAAGATC 
AAGAGAATGT TAGAAAATTT TGAACGCGTG ACCGTGAATT CCATTTTAAA TTTATCACGT 
GTAAAGGGGC CAGAACAAAC TGACCTTGGT GAAATAAAAC AAACTGCTGA CAAAAGAGGA 
CAAAACAGTA ATGCCCCAGC CACTCTGAAG AAAGAAGATA TAAGTTCTAC TTCTGAGGAA 
AAAGTAGATG ATAAACGTAT TGCTGATATG GCTGAAGCAT TTTGTAGCCA TTTGGGAGCT 
GAAACTTCAA AAATGTATCC AGACATTTTA CTTAATTCTA CAGATTCAGT GATGAATATT 
CAGACGTCAG GTCATGGTAA AGAGGAACAG TGGGATAGTG TAGTTTCTGA AGAGGACATA 
TATACCAGCC AGAATGCCGA AACCACTGAT GATGATGCCT TTTTACATCA GATGTCTCCT 
GAAAATGAAA TCATCATTAA AGAAAATGTT TCACCTACAG AGGATGCCTT GGTTTTACCT 
GGGCTAATGC CAGAGTTGCT AAACTTTGTT GGAGACTGGC CAGTTGAGCA CACTATGGGT 
CAGAGAGCCC CACGGAGTAG GAGCAAGATT AGAGCAAATT CTACAAGTAA TGAATCATGT 
TCCCTCCAGC TCAGTGAGTT GACAAATGGT GATTCTAAAG AACTGGATAG TCACACTATA 
ACACATGATT TGTTAAATCT AAGCAAAGAA ATCGATAAAT GTACCATTTT ACAAACAGAT 
TATCTTGGAG ATTTGCCAAA TTTGTGTTTT GGGTGTAACG AAGAAAATAT GCAATTAGAA 
GATCAAGTCT GCTCAGCTTT AGAAAGAGAG GCAGAGACGT ACATACCTCA TACAGTACAG 
AACTCTGAAT CAAATCATGT TGAGAAAGAT CCACAAGCAG TCACTGAAGT TACTAAAGAT 
TTGCAAAGCC TTGATATATC TAATATTAAC GAAAATGAAC TTTCAGAATG CGAAACACCA 
GAGAAAAAAA CAAGGCAATG CAAACGAAAT GTTAGACAGT GTAAACTGGC TTTAACTTTT 
ACAAATAGCT GTTCTGACTC TTCCCAAACA GAAGATATAC TGACATATCA ACCAAATGCT 
CCAGTAAATG AAGTCTACTG TGGACCTAGT AAATGTTCGC AAACTGAGCC TCATGAATTT 
GCTTTAGCCT GGAGGATAGA GAAAAAAAAT ATTGACATCT CCAATTCGGC GAAAGTCCTC 
ACTGGCAAAG GTGAACGTTT TCAGTCAAAA TTAATGGATG AAAGTTCAGT TTCACAGGAA 
ACTATTCCCT ATAGAGCAAT GCATCATAAA AGTACATTTG TGGAAGAAGA TGAAATAGTT 
TGCTTGGATG ATGAACATAA TCTAAATATC CTTGCTGGAC TCTTTAGGTC AGTTTCATTT 
GACGTGCTTA TTGATCTTTT TGAAAGATGC AATAAGGACA TTGTGTGGGC ATCCAACTTA 
CTCTTGGATT CCGGGGAGAA AATCTACAAA GATGAAGACT GCCAGACAGA GGATTATTTG 
AGTGTTGTAG GAGATGCCAC TTTGGGAACT GGTCATCGTC CAGACCAAGT TCCTGTATGC 
CCTGATGATC TTAATGCTCC ATCTTTTGGT AGTAGTATGT CACATACTGA TGCTTGTTCC 
AGCACACCAC TAAATGAAAA AAGAGAGCAT GCTGATAGTC ACACAGTTAT AGAAAATACC 
AACCTAACAT ATTCAGAGGC CCTAAGTTCA GAAGATTCAA CAAATCTAAA TGTGTTCTCT 
GTCCCTGATA TAACAAATGA ACAAACCATA GAACAACATA AAATGGTTAC AAAATCATTG 
AATATTGAGC AGCCAAAAGA TACTGATTTG CAACCAGTGT CTAGGATATG CTTATCTGAT 
ATAGATATGG AGGCTTCTCA GAAAACAGCT GATGATATTG ACACAGGAAT CAATGTTTTA 
GAAACACACA ACATTGATAA AGATGTTTGT GAACTTCCCT TGTACGATAT TAATGTACAG 
GACAAAATAC GTGGATTTGC TCTAGAAGAG GTGGATGATG CAAAAAAGAA AAACTCTGAT 
TCCCTCAAAG ACTCTTTGCA TTTTGATCAT CTGGAATTGT GTTTGCCTCC AGAACTAGCC 
TTTCAGCTCA GTGAATTGTT TGGCCCTGTA GGAATAGCTC CAGGTTCTTT AACTATTGAG 
GATTGTACTG TACACATAGA CTTAAATCTG GCCAAAGCTA TCCATACAAG ATGGAAAGAA 
TCTATTACGG AGAGGCAGAA ACAAGAATCC TTGTCTTACC AGCTGCTTTT TGAAGATTTA 
TCGCCTTCAG AACTGGATAA TCTGATAAAT GAAGAGGAAT CTAACTTGGC AAAACCTGAA 
TATTCCGATA CATCGTCTGA CACATTCCCT TTCATGGATC AGTGGAATGT ACGAACCAAG 
AAAGTGTCAT TACGCGAAAT AATGTCAGAG GAAATTGCAT TACAGGAACA AGAATGTCAG 
AAGTCTACAC TAAGGACAAA AAACTGTGCT GCAAAACTGA AGGAAAAACA ACTTTTTGAA 
TTGTTTCCTA ACATTGAACA AAGGCTTCTT ATGGATATTT TTAAGGAAAA TGATTATTCT 
TTAGAGAGGA CTGCAGAGTT CCTAAGTGTC GTTCTGGAAG CTGGCCCAGT GCAGAATATT 
GTTGCCTGTG ATTTAAAACA AACAGATGCC TCTACCTCTG AAAACCCTAA ATCAAAGAAA 
TTAAATCTGG ACAAAAAAAC CATCCCTGAG AATTTCTATC AGGACTTTGA ATGCCCAGAG 
TATGATGATT TCCGGGCTGA AGCTTTCCTT TACCGTAGAA AGCAACAAGA GTGTTACAGG 
AAAGCAAGCG AGGCTCACAG CAGAGGCATG AAACAAGTTG CAACATATTA TGCACAACAG 
GGGTACCTCT ATGGCCAGAA GATGAAACAG GAAAACCACA GAGCTGCTGT ACAGATCTTT 
GAAAGAACTA ATGAATTCTT GTTGCCAGAG AATATATTAG ACCTGCACGG GCTCCATGTT 
AGTGAGGCAA TGAAACAATT GCGGCAGGTA CTTCAGAACA AGATGGAAGA TTATAAAAAG 
AATGGTGGCA AATCTTACCT TTCCGTGATA ACTGGAAGAG GAAATCATAG CCAAGGAGGA 
GTCCCTCGCA TTAAATTAGC AGTCATTGAC TATCTCACAA ACCACAGCTT CAGGTTTCAG 
GAGGTAAGGC CGGGAGTGCT GCATATCACC CTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950886.1
CDS	166..5004
Translation	MPRKRKTHSLSPLRKPSSSSSTTDRPSSVALPLPPTMSNNNNEGLLDSMCDMFPDLDPSLVEMVLSEYKEVEVVMDYLLELSTSAKVETNTESANVLGFDSVAAVLHHSKKPLVGSKTLEDTAHQHSDIYVAQDVSQAQDCTAVSNNLDLLLDEAINKYSLYSSIDLAAEPEQCHIPDNSNIPAQSQSTSAIISSILADEDNEMLKDLESDDQQTAINQTVPSINKQYTASIDPNFALQLPNESGLKDSGMELTAKLDIAKADSCCILEPASGSLERNGRSKTASEEKSNHDETEAGITPLSHANSMGSWQNSHLQKSPLWNPLAPSFCPAAGLQHRFITPIHVSRAPWNFTGGVRNVSRDFYYRPPVPSFTWSGNNFSPNVWQTASIKSAALLEAVPQPAVQNVKKNTFFVGKVLILLRGAPGSGKTTLARMLLQQNPLGINLSTDEYFYKKGQYRYDVNLLGEAHEWNHKRAKEAFEKNISPIIIDNTNLQAWEMKPYVSMAMKHKYKVTFREPDTWWKYKPKELERRNSHGVTKEKIKRMLENFERVTVNSILNLSRVKGPEQTDLGEIKQTADKRGQNSNAPATLKKEDISSTSEEKVDDKRIADMAEAFCSHLGAETSKMYPDILLNSTDSVMNIQTSGHGKEEQWDSVVSEEDIYTSQNAETTDDDAFLHQMSPENEIIIKENVSPTEDALVLPGLMPELLNFVGDWPVEHTMGQRAPRSRSKIRANSTSNESCSLQLSELTNGDSKELDSHTITHDLLNLSKEIDKCTILQTDYLGDLPNLCFGCNEENMQLEDQVCSALEREAETYIPHTVQNSESNHVEKDPQAVTEVTKDLQSLDISNINENELSECETPEKKTRQCKRNVRQCKLALTFTNSCSDSSQTEDILTYQPNAPVNEVYCGPSKCSQTEPHEFALAWRIEKKNIDISNSAKVLTGKGERFQSKLMDESSVSQETIPYRAMHHKSTFVEEDEIVCLDDEHNLNILAGLFRSVSFDVLIDLFERCNKDIVWASNLLLDSGEKIYKDEDCQTEDYLSVVGDATLGTGHRPDQVPVCPDDLNAPSFGSSMSHTDACSSTPLNEKREHADSHTVIENTNLTYSEALSSEDSTNLNVFSVPDITNEQTIEQHKMVTKSLNIEQPKDTDLQPVSRICLSDIDMEASQKTADDIDTGINVLETHNIDKDVCELPLYDINVQDKIRGFALEEVDDAKKKNSDSLKDSLHFDHLELCLPPELAFQLSELFGPVGIAPGSLTIEDCTVHIDLNLAKAIHTRWKESITERQKQESLSYQLLFEDLSPSELDNLINEEESNLAKPEYSDTSSDTFPFMDQWNVRTKKVSLREIMSEEIALQEQECQKSTLRTKNCAAKLKEKQLFELFPNIEQRLLMDIFKENDYSLERTAEFLSVVLEAGPVQNIVACDLKQTDASTSENPKSKKLNLDKKTIPENFYQDFECPEYDDFRAEAFLYRRKQQECYRKASEAHSRGMKQVATYYAQQGYLYGQKMKQENHRAAVQIFERTNEFLLPENILDLHGLHVSEAMKQLRQVLQNKMEDYKKNGGKSYLSVITGRGNHSQGGVPRIKLAVIDYLTNHSFRFQEVRPGVLHITLK

Target ORF information:

RefSeq Version	XM_018095397.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095397.2

ORF Insert Sequence:

ATGCCAAGAA AAAGAAAAAC TCATTCCTTG AGTCCATTAA GGAAGCCTTC ATCATCTTCC 
TCTACCACTG ATCGTCCATC CTCTGTGGCA TTACCTCTAC CTCCAACCAT GAGTAATAAC 
AACAATGAGG GCCTGCTGGA CAGCATGTGT GACATGTTTC CCGATCTTGA TCCTTCCTTG 
GTTGAAATGG TTCTTTCTGA ATACAAAGAA GTTGAAGTTG TGATGGATTA TCTCCTGGAA 
CTATCAACTT CAGCAAAAGT GGAGACCAAT ACAGAATCGG CCAACGTTTT GGGATTTGAT 
AGTGTAGCAG CTGTTTTGCA CCACAGCAAA AAACCTTTGG TTGGTTCTAA AACTTTGGAA 
GATACTGCTC ATCAGCATTC TGACATTTAT GTAGCTCAGG ATGTATCTCA GGCTCAGGAC 
TGCACTGCTG TGTCTAATAA CTTGGATTTA CTCTTGGATG AGGCCATTAA CAAATATAGT 
CTGTATAGTA GTATAGATCT TGCAGCTGAG CCTGAGCAGT GTCATATACC AGACAACAGT 
AACATTCCAG CTCAAAGTCA GTCTACTTCA GCCATAATCT CCTCTATACT TGCCGATGAA 
GATAATGAAA TGCTAAAAGA CCTTGAAAGT GATGACCAAC AAACTGCCAT AAACCAAACA 
GTTCCATCTA TTAATAAACA ATACACTGCA AGCATCGACC CTAATTTTGC TTTACAGCTT 
CCAAATGAAT CAGGGCTGAA AGACTCTGGG ATGGAATTAA CTGCAAAACT AGATATAGCC 
AAGGCAGATT CCTGCTGCAT TCTAGAGCCT GCATCAGGCT CTTTAGAACG GAATGGGAGG 
AGTAAAACCG CAAGTGAAGA AAAGAGCAAC CATGATGAAA CTGAAGCAGG AATTACACCT 
CTGTCACATG CTAACTCCAT GGGATCTTGG CAAAATAGTC ACCTACAAAA ATCACCCCTT 
TGGAATCCTT TGGCACCTTC ATTTTGCCCA GCAGCAGGTT TACAGCATAG ATTTATAACT 
CCAATACATG TTAGCCGGGC ACCATGGAAT TTCACTGGAG GTGTAAGAAA TGTTAGTAGA 
GATTTCTATT ATAGACCCCC AGTGCCTTCA TTTACATGGA GTGGTAACAA CTTCTCTCCA 
AATGTTTGGC AAACTGCCAG TATAAAATCA GCAGCTCTTT TGGAAGCTGT TCCCCAACCT 
GCTGTTCAAA ATGTGAAAAA AAATACATTT TTTGTGGGAA AAGTTCTCAT TCTTCTTCGA 
GGTGCACCAG GATCAGGAAA AACTACTTTA GCCAGGATGT TGTTACAGCA GAACCCATTG 
GGTATTAACC TAAGTACTGA TGAGTATTTT TACAAAAAGG GGCAATACCG ATATGATGTA 
AATCTGTTAG GGGAAGCACA TGAGTGGAAT CATAAACGAG CCAAAGAAGC CTTTGAGAAG 
AATATTTCCC CAATTATAAT AGATAATACC AACTTGCAAG CTTGGGAAAT GAAGCCATAT 
GTGTCTATGG CCATGAAACA CAAGTACAAA GTCACATTTC GTGAACCTGA TACTTGGTGG 
AAGTATAAAC CAAAAGAACT GGAAAGGCGT AACAGTCATG GAGTAACTAA AGAGAAGATC 
AAGAGAATGT TAGAAAATTT TGAACGCGTG ACCGTGAATT CCATTTTAAA TTTATCACGT 
GTAAAGGGGC CAGAACAAAC TGACCTTGGT GAAATAAAAC AAACTGCTGA CAAAAGAGGA 
CAAAACAGTA ATGCCCCAGC CACTCTGAAG AAAGAAGATA TAAGTTCTAC TTCTGAGGAA 
AAAGTAGATG ATAAACGTAT TGCTGATATG GCTGAAGCAT TTTGTAGCCA TTTGGGAGCT 
GAAACTTCAA AAATGTATCC AGACATTTTA CTTAATTCTA CAGATTCAGT GATGAATATT 
CAGACGTCAG GTCATGGTAA AGAGGAACAG TGGGATAGTG TAGTTTCTGA AGAGGACATA 
TATACCAGCC AGAATGCCGA AACCACTGAT GATGATGCCT TTTTACATCA GATGTCTCCT 
GAAAATGAAA TCATCATTAA AGAAAATGTT TCACCTACAG AGGATGCCTT GGTTTTACCT 
GGGCTAATGC CAGAGTTGCT AAACTTTGTT GGAGACTGGC CAGTTGAGCA CACTATGGGT 
CAGAGAGCCC CACGGAGTAG GAGCAAGATT AGAGCAAATT CTACAAGTAA TGAATCATGT 
TCCCTCCAGC TCAGTGAGTT GACAAATGGT GATTCTAAAG AACTGGATAG TCACACTATA 
ACACATGATT TGTTAAATCT AAGCAAAGAA ATCGATAAAT GTACCATTTT ACAAACAGAT 
TATCTTGGAG ATTTGCCAAA TTTGTGTTTT GGGTGTAACG AAGAAAATAT GCAATTAGAA 
GATCAAGTCT GCTCAGCTTT AGAAAGAGAG GCAGAGACGT ACATACCTCA TACAGTACAG 
AACTCTGAAT CAAATCATGT TGAGAAAGAT CCACAAGCAG TCACTGAAGT TACTAAAGAT 
TTGCAAAGCC TTGATATATC TAATATTAAC GAAAATGAAC TTTCAGAATG CGAAACACCA 
GAGAAAAAAA CAAGGCAATG CAAACGAAAT GTTAGACAGT GTAAACTGGC TTTAACTTTT 
ACAAATAGCT GTTCTGACTC TTCCCAAACA GAAGATATAC TGACATATCA ACCAAATGCT 
CCAGTAAATG AAGTCTACTG TGGACCTAGT AAATGTTCGC AAACTGAGCC TCATGAATTT 
GCTTTAGCCT GGAGGATAGA GAAAAAAAAT ATTGACATCT CCAATTCGGC GAAAGTCCTC 
ACTGGCAAAG GTGAACGTTT TCAGTCAAAA TTAATGGATG AAAGTTCAGT TTCACAGGAA 
ACTATTCCCT ATAGAGCAAT GCATCATAAA AGTACATTTG TGGAAGAAGA TGAAATAGTT 
TGCTTGGATG ATGAACATAA TCTAAATATC CTTGCTGGAC TCTTTAGGTC AGTTTCATTT 
GACGTGCTTA TTGATCTTTT TGAAAGATGC AATAAGGACA TTGTGTGGGC ATCCAACTTA 
CTCTTGGATT CCGGGGAGAA AATCTACAAA GATGAAGACT GCCAGACAGA GGATTATTTG 
AGTGTTGTAG GAGATGCCAC TTTGGGAACT GGTCATCGTC CAGACCAAGT TCCTGTATGC 
CCTGATGATC TTAATGCTCC ATCTTTTGGT AGTAGTATGT CACATACTGA TGCTTGTTCC 
AGCACACCAC TAAATGAAAA AAGAGAGCAT GCTGATAGTC ACACAGTTAT AGAAAATACC 
AACCTAACAT ATTCAGAGGC CCTAAGTTCA GAAGATTCAA CAAATCTAAA TGTGTTCTCT 
GTCCCTGATA TAACAAATGA ACAAACCATA GAACAACATA AAATGGTTAC AAAATCATTG 
AATATTGAGC AGCCAAAAGA TACTGATTTG CAACCAGTGT CTAGGATATG CTTATCTGAT 
ATAGATATGG AGGCTTCTCA GAAAACAGCT GATGATATTG ACACAGGAAT CAATGTTTTA 
GAAACACACA ACATTGATAA AGATGTTTGT GAACTTCCCT TGTACGATAT TAATGTACAG 
GACAAAATAC GTGGATTTGC TCTAGAAGAG GTGGATGATG CAAAAAAGAA AAACTCTGAT 
TCCCTCAAAG ACTCTTTGCA TTTTGATCAT CTGGAATTGT GTTTGCCTCC AGAACTAGCC 
TTTCAGCTCA GTGAATTGTT TGGCCCTGTA GGAATAGCTC CAGGTTCTTT AACTATTGAG 
GATTGTACTG TACACATAGA CTTAAATCTG GCCAAAGCTA TCCATACAAG ATGGAAAGAA 
TCTATTACGG AGAGGCAGAA ACAAGAATCC TTGTCTTACC AGCTGCTTTT TGAAGATTTA 
TCGCCTTCAG AACTGGATAA TCTGATAAAT GAAGAGGAAT CTAACTTGGC AAAACCTGAA 
TATTCCGATA CATCGTCTGA CACATTCCCT TTCATGGATC AGTGGAATGT ACGAACCAAG 
AAAGTGTCAT TACGCGAAAT AATGTCAGAG GAAATTGCAT TACAGGAACA AGAATGTCAG 
AAGTCTACAC TAAGGACAAA AAACTGTGCT GCAAAACTGA AGGAAAAACA ACTTTTTGAA 
TTGTTTCCTA ACATTGAACA AAGGCTTCTT ATGGATATTT TTAAGGAAAA TGATTATTCT 
TTAGAGAGGA CTGCAGAGTT CCTAAGTGTC GTTCTGGAAG CTGGCCCAGT GCAGAATATT 
GTTGCCTGTG ATTTAAAACA AACAGATGCC TCTACCTCTG AAAACCCTAA ATCAAAGAAA 
TTAAATCTGG ACAAAAAAAC CATCCCTGAG AATTTCTATC AGGACTTTGA ATGCCCAGAG 
TATGATGATT TCCGGGCTGA AGCTTTCCTT TACCGTAGAA AGCAACAAGA GTGTTACAGG 
AAAGCAAGCG AGGCTCACAG CAGAGGCATG AAACAAGTTG CAACATATTA TGCACAACAG 
GGGTACCTCT ATGGCCAGAA GATGAAACAG GAAAACCACA GAGCTGCTGT ACAGATCTTT 
GAAAGAACTA ATGAATTCTT GTTGCCAGAG AATATATTAG ACCTGCACGG GCTCCATGTT 
AGTGAGGCAA TGAAACAATT GCGGCAGGTA CTTCAGAACA AGATGGAAGA TTATAAAAAG 
AATGGTGGCA AATCTTACCT TTCCGTGATA ACTGGAAGAG GAAATCATAG CCAAGGAGGA 
GTCCCTCGCA TTAAATTAGC AGTCATTGAC TATCTCACAA ACCACAGCTT CAGGTTTCAG 
GAGGTAAGGC CGGGAGTGCT GCATATCACC CTGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

n4bp2 ( XM_002933469.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933515.2 Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X1, mRNA.

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CloneID	OXa04165
Clone ID Related Accession (Same CDS sequence)	XM_002933469.4 , XM_002933469.5
Accession Version	XM_002933469.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933469.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGAA AAAGAAAAAC TCATTCCTTG AGTCCATTAA GGAAGCCTTC ATCATCTTCC 
TCTACCACTG ATCGTCCATC CTCTGTGGCA TTACCTCTAC CTCCAACCAT GAGTAATAAC 
AACAATGAGG GCCTGCTGGA CAGCATGTGT GACATGTTTC CCGATCTTGA TCCTTCCTTG 
GTTGAAATGG TTCTTTCTGA ATACAAAGAA GTTGAAGTTG TGATGGATTA TCTCCTGGAA 
CTATCAACTT CAGCAAAAGT GGAGACCAAT ACAGAATCGG CCAACGTTTT GGGATTTGAT 
AGTGTAGCAG CTGTTTTGCA CCACAGCAAA AAACCTTTGG TTGGTTCTAA AACTTTGGAA 
GATACTGCTC ATCAGCATTC TGACATTTAT GTAGCTCAGG ATGTATCTCA GGCTCAGGAC 
TGCACTGCTG TGTCTAATAA CTTGGATTTA CTCTTGGATG AGGCCATTAA CAAATATAGT 
CTGTATAGTA GTATAGATCT TGCAGCTGAG CCTGAGCAGT GTCATATACC AGACAACAGT 
AACATTCCAG CTCAAAGTCA GTCTACTTCA GCCATAATCT CCTCTATACT TGCCGATGAA 
GATAATGAAA TGCTAAAAGA CCTTGAAAGT GATGACCAAC AAACTGCCAT AAACCAAACA 
GTTCCATCTA TTAATAAACA ATACACTGCA AGCATCGACC CTAATTTTGC TTTACAGCTT 
CCAAATGAAT CAGGGCTGAA AGACTCTGGG ATGGAATTAA CTGCAAAACT AGATATAGCC 
AAGGCAGATT CCTGCTGCAT TCTAGAGCCT GCATCAGGCT CTTTAGAACG GAATGGGAGG 
AGTAAAACCG CAAGTGAAGA AAAGAGCAAC CATGATGAAA CTGAAGCAGG AATTACACCT 
CTGTCACATG CTAACTCCAT GGGATCTTGG CAAAATAGTC ACCTACAAAA ATCACCCCTT 
TGGAATCCTT TGGCACCTTC ATTTTGCCCA GCAGCAGGTT TACAGCATAG ATTTATAACT 
CCAATACATG TTAGCCGGGC ACCATGGAAT TTCACTGGAG GTGTAAGAAA TGTTAGTAGA 
GATTTCTATT ATAGACCCCC AGTGCCTTCA TTTACATGGA GTGGTAACAA CTTCTCTCCA 
AATGTTTGGC AAACTGCCAG TATAAAATCA GCAGCTCTTT TGGAAGCTGT TCCCCAACCT 
GCTGTTCAAA ATGTGAAAAA AAATACATTT TTTGTGGGAA AAGTTCTCAT TCTTCTTCGA 
GGTGCACCAG GATCAGGAAA AACTACTTTA GCCAGGATGT TGTTACAGCA GAACCCATTG 
GGTATTAACC TAAGTACTGA TGAGTATTTT TACAAAAAGG GGCAATACCG ATATGATGTA 
AATCTGTTAG GGGAAGCACA TGAGTGGAAT CATAAACGAG CCAAAGAAGC CTTTGAGAAG 
AATATTTCCC CAATTATAAT AGATAATACC AACTTGCAAG CTTGGGAAAT GAAGCCATAT 
GTGTCTATGG CCATGAAACA CAAGTACAAA GTCACATTTC GTGAACCTGA TACTTGGTGG 
AAGTATAAAC CAAAAGAACT GGAAAGGCGT AACAGTCATG GAGTAACTAA AGAGAAGATC 
AAGAGAATGT TAGAAAATTT TGAACGCGTG ACCGTGAATT CCATTTTAAA TTTATCACGT 
GTAAAGGGGC CAGAACAAAC TGACCTTGGT GAAATAAAAC AAACTGCTGA CAAAAGAGGA 
CAAAACAGTA ATGCCCCAGC CACTCTGAAG AAAGAAGATA TAAGTTCTAC TTCTGAGGAA 
AAAGTAGATG ATAAACGTAT TGCTGATATG GCTGAAGCAT TTTGTAGCCA TTTGGGAGCT 
GAAACTTCAA AAATGTATCC AGACATTTTA CTTAATTCTA CAGATTCAGT GATGAATATT 
CAGACGTCAG GTCATGGTAA AGAGGAACAG TGGGATAGTG TAGTTTCTGA AGAGGACATA 
TATACCAGCC AGAATGCCGA AACCACTGAT GATGATGCCT TTTTACATCA GATGTCTCCT 
GAAAATGAAA TCATCATTAA AGAAAATGTT TCACCTACAG AGGATGCCTT GGTTTTACCT 
GGGCTAATGC CAGAGTTGCT AAACTTTGTT GGAGACTGGC CAGTTGAGCA CACTATGGGT 
CAGAGAGCCC CACGGAGTAG GAGCAAGATT AGAGCAAATT CTACAAGTAA TGAATCATGT 
TCCCTCCAGC TCAGTGAGTT GACAAATGGT GATTCTAAAG AACTGGATAG TCACACTATA 
ACACATGATT TGTTAAATCT AAGCAAAGAA ATCGATAAAT GTACCATTTT ACAAACAGAT 
TATCTTGGAG ATTTGCCAAA TTTGTGTTTT GGGTGTAACG AAGAAAATAT GCAATTAGAA 
GATCAAGTCT GCTCAGCTTT AGAAAGAGAG GCAGAGACGT ACATACCTCA TACAGTACAG 
AACTCTGAAT CAAATCATGT TGAGAAAGAT CCACAAGCAG TCACTGAAGT TACTAAAGAT 
TTGCAAAGCC TTGATATATC TAATATTAAC GAAAATGAAC TTTCAGAATG CGAAACACCA 
GAGAAAAAAA CAAGGCAATG CAAACGAAAT GTTAGACAGT GTAAACTGGC TTTAACTTTT 
ACAAATAGCT GTTCTGACTC TTCCCAAACA GAAGATATAC TGACATATCA ACCAAATGCT 
CCAGTAAATG AAGTCTACTG TGGACCTAGT AAATGTTCGC AAACTGAGCC TCATGAATTT 
GCTTTAGCCT GGAGGATAGA GAAAAAAAAT ATTGACATCT CCAATTCGGC GAAAGTCCTC 
ACTGGCAAAG GTGAACGTTT TCAGTCAAAA TTAATGGATG AAAGTTCAGT TTCACAGGAA 
ACTATTCCCT ATAGAGCAAT GCATCATAAA AGTACATTTG TGGAAGAAGA TGAAATAGTT 
TGCTTGGATG ATGAACATAA TCTAAATATC CTTGCTGGAC TCTTTAGGTC AGTTTCATTT 
GACGTGCTTA TTGATCTTTT TGAAAGATGC AATAAGGACA TTGTGTGGGC ATCCAACTTA 
CTCTTGGATT CCGGGGAGAA AATCTACAAA GATGAAGACT GCCAGACAGA GGATTATTTG 
AGTGTTGTAG GAGATGCCAC TTTGGGAACT GGTCATCGTC CAGACCAAGT TCCTGTATGC 
CCTGATGATC TTAATGCTCC ATCTTTTGGT AGTAGTATGT CACATACTGA TGCTTGTTCC 
AGCACACCAC TAAATGAAAA AAGAGAGCAT GCTGATAGTC ACACAGTTAT AGAAAATACC 
AACCTAACAT ATTCAGAGGC CCTAAGTTCA GAAGATTCAA CAAATCTAAA TGTGTTCTCT 
GTCCCTGATA TAACAAATGA ACAAACCATA GAACAACATA AAATGGTTAC AAAATCATTG 
AATATTGAGC AGCCAAAAGA TACTGATTTG CAACCAGTGT CTAGGATATG CTTATCTGAT 
ATAGATATGG AGGCTTCTCA GAAAACAGCT GATGATATTG ACACAGGAAT CAATGTTTTA 
GAAACACACA ACATTGATAA AGATGTTTGT GAACTTCCCT TGTACGATAT TAATGTACAG 
GACAAAATAC GTGGATTTGC TCTAGAAGAG GTGGATGATG CAAAAAAGAA AAACTCTGAT 
TCCCTCAAAG ACTCTTTGCA TTTTGATCAT CTGGAATTGT GTTTGCCTCC AGAACTAGCC 
TTTCAGCTCA GTGAATTGTT TGGCCCTGTA GGAATAGCTC CAGGTTCTTT AACTATTGAG 
GATTGTACTG TACACATAGA CTTAAATCTG GCCAAAGCTA TCCATACAAG ATGGAAAGAA 
TCTATTACGG AGAGGCAGAA ACAAGAATCC TTGTCTTACC AGCTGCTTTT TGAAGATTTA 
TCGCCTTCAG AACTGGATAA TCTGATAAAT GAAGAGGAAT CTAACTTGGC AAAACCTGAA 
TATTCCGATA CATCGTCTGA CACATTCCCT TTCATGGATC AGTGGAATGT ACGAACCAAG 
AAAGTGTCAT TACGCGAAAT AATGTCAGAG GAAATTGCAT TACAGGAACA AGAATGTCAG 
AAGAAGTCTA CACTAAGGAC AAAAAACTGT GCTGCAAAAC TGAAGGAAAA ACAACTTTTT 
GAATTGTTTC CTAACATTGA ACAAAGGCTT CTTATGGATA TTTTTAAGGA AAATGATTAT 
TCTTTAGAGA GGACTGCAGA GTTCCTAAGT GTCGTTCTGG AAGCTGGCCC AGTGCAGAAT 
ATTGTTGCCT GTGATTTAAA ACAAACAGAT GCCTCTACCT CTGAAAACCC TAAATCAAAG 
AAATTAAATC TGGACAAAAA AACCATCCCT GAGAATTTCT ATCAGGACTT TGAATGCCCA 
GAGTATGATG ATTTCCGGGC TGAAGCTTTC CTTTACCGTA GAAAGCAACA AGAGTGTTAC 
AGGAAAGCAA GCGAGGCTCA CAGCAGAGGC ATGAAACAAG TTGCAACATA TTATGCACAA 
CAGGGGTACC TCTATGGCCA GAAGATGAAA CAGGAAAACC ACAGAGCTGC TGTACAGATC 
TTTGAAAGAA CTAATGAATT CTTGTTGCCA GAGAATATAT TAGACCTGCA CGGGCTCCAT 
GTTAGTGAGG CAATGAAACA ATTGCGGCAG GTACTTCAGA ACAAGATGGA AGATTATAAA 
AAGAATGGTG GCAAATCTTA CCTTTCCGTG ATAACTGGAA GAGGAAATCA TAGCCAAGGA 
GGAGTCCCTC GCATTAAATT AGCAGTCATT GACTATCTCA CAAACCACAG CTTCAGGTTT 
CAGGAGGTAA GGCCGGGAGT GCTGCATATC ACCCTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933515.2
CDS	822..5663
Translation	MPRKRKTHSLSPLRKPSSSSSTTDRPSSVALPLPPTMSNNNNEGLLDSMCDMFPDLDPSLVEMVLSEYKEVEVVMDYLLELSTSAKVETNTESANVLGFDSVAAVLHHSKKPLVGSKTLEDTAHQHSDIYVAQDVSQAQDCTAVSNNLDLLLDEAINKYSLYSSIDLAAEPEQCHIPDNSNIPAQSQSTSAIISSILADEDNEMLKDLESDDQQTAINQTVPSINKQYTASIDPNFALQLPNESGLKDSGMELTAKLDIAKADSCCILEPASGSLERNGRSKTASEEKSNHDETEAGITPLSHANSMGSWQNSHLQKSPLWNPLAPSFCPAAGLQHRFITPIHVSRAPWNFTGGVRNVSRDFYYRPPVPSFTWSGNNFSPNVWQTASIKSAALLEAVPQPAVQNVKKNTFFVGKVLILLRGAPGSGKTTLARMLLQQNPLGINLSTDEYFYKKGQYRYDVNLLGEAHEWNHKRAKEAFEKNISPIIIDNTNLQAWEMKPYVSMAMKHKYKVTFREPDTWWKYKPKELERRNSHGVTKEKIKRMLENFERVTVNSILNLSRVKGPEQTDLGEIKQTADKRGQNSNAPATLKKEDISSTSEEKVDDKRIADMAEAFCSHLGAETSKMYPDILLNSTDSVMNIQTSGHGKEEQWDSVVSEEDIYTSQNAETTDDDAFLHQMSPENEIIIKENVSPTEDALVLPGLMPELLNFVGDWPVEHTMGQRAPRSRSKIRANSTSNESCSLQLSELTNGDSKELDSHTITHDLLNLSKEIDKCTILQTDYLGDLPNLCFGCNEENMQLEDQVCSALEREAETYIPHTVQNSESNHVEKDPQAVTEVTKDLQSLDISNINENELSECETPEKKTRQCKRNVRQCKLALTFTNSCSDSSQTEDILTYQPNAPVNEVYCGPSKCSQTEPHEFALAWRIEKKNIDISNSAKVLTGKGERFQSKLMDESSVSQETIPYRAMHHKSTFVEEDEIVCLDDEHNLNILAGLFRSVSFDVLIDLFERCNKDIVWASNLLLDSGEKIYKDEDCQTEDYLSVVGDATLGTGHRPDQVPVCPDDLNAPSFGSSMSHTDACSSTPLNEKREHADSHTVIENTNLTYSEALSSEDSTNLNVFSVPDITNEQTIEQHKMVTKSLNIEQPKDTDLQPVSRICLSDIDMEASQKTADDIDTGINVLETHNIDKDVCELPLYDINVQDKIRGFALEEVDDAKKKNSDSLKDSLHFDHLELCLPPELAFQLSELFGPVGIAPGSLTIEDCTVHIDLNLAKAIHTRWKESITERQKQESLSYQLLFEDLSPSELDNLINEEESNLAKPEYSDTSSDTFPFMDQWNVRTKKVSLREIMSEEIALQEQECQKKSTLRTKNCAAKLKEKQLFELFPNIEQRLLMDIFKENDYSLERTAEFLSVVLEAGPVQNIVACDLKQTDASTSENPKSKKLNLDKKTIPENFYQDFECPEYDDFRAEAFLYRRKQQECYRKASEAHSRGMKQVATYYAQQGYLYGQKMKQENHRAAVQIFERTNEFLLPENILDLHGLHVSEAMKQLRQVLQNKMEDYKKNGGKSYLSVITGRGNHSQGGVPRIKLAVIDYLTNHSFRFQEVRPGVLHITLK

Target ORF information:

RefSeq Version	XM_002933469.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NEDD4 binding protein 2 (n4bp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933469.4

ORF Insert Sequence:

ATGCCAAGAA AAAGAAAAAC TCATTCCTTG AGTCCATTAA GGAAGCCTTC ATCATCTTCC 
TCTACCACTG ATCGTCCATC CTCTGTGGCA TTACCTCTAC CTCCAACCAT GAGTAATAAC 
AACAATGAGG GCCTGCTGGA CAGCATGTGT GACATGTTTC CCGATCTTGA TCCTTCCTTG 
GTTGAAATGG TTCTTTCTGA ATACAAAGAA GTTGAAGTTG TGATGGATTA TCTCCTGGAA 
CTATCAACTT CAGCAAAAGT GGAGACCAAT ACAGAATCGG CCAACGTTTT GGGATTTGAT 
AGTGTAGCAG CTGTTTTGCA CCACAGCAAA AAACCTTTGG TTGGTTCTAA AACTTTGGAA 
GATACTGCTC ATCAGCATTC TGACATTTAT GTAGCTCAGG ATGTATCTCA GGCTCAGGAC 
TGCACTGCTG TGTCTAATAA CTTGGATTTA CTCTTGGATG AGGCCATTAA CAAATATAGT 
CTGTATAGTA GTATAGATCT TGCAGCTGAG CCTGAGCAGT GTCATATACC AGACAACAGT 
AACATTCCAG CTCAAAGTCA GTCTACTTCA GCCATAATCT CCTCTATACT TGCCGATGAA 
GATAATGAAA TGCTAAAAGA CCTTGAAAGT GATGACCAAC AAACTGCCAT AAACCAAACA 
GTTCCATCTA TTAATAAACA ATACACTGCA AGCATCGACC CTAATTTTGC TTTACAGCTT 
CCAAATGAAT CAGGGCTGAA AGACTCTGGG ATGGAATTAA CTGCAAAACT AGATATAGCC 
AAGGCAGATT CCTGCTGCAT TCTAGAGCCT GCATCAGGCT CTTTAGAACG GAATGGGAGG 
AGTAAAACCG CAAGTGAAGA AAAGAGCAAC CATGATGAAA CTGAAGCAGG AATTACACCT 
CTGTCACATG CTAACTCCAT GGGATCTTGG CAAAATAGTC ACCTACAAAA ATCACCCCTT 
TGGAATCCTT TGGCACCTTC ATTTTGCCCA GCAGCAGGTT TACAGCATAG ATTTATAACT 
CCAATACATG TTAGCCGGGC ACCATGGAAT TTCACTGGAG GTGTAAGAAA TGTTAGTAGA 
GATTTCTATT ATAGACCCCC AGTGCCTTCA TTTACATGGA GTGGTAACAA CTTCTCTCCA 
AATGTTTGGC AAACTGCCAG TATAAAATCA GCAGCTCTTT TGGAAGCTGT TCCCCAACCT 
GCTGTTCAAA ATGTGAAAAA AAATACATTT TTTGTGGGAA AAGTTCTCAT TCTTCTTCGA 
GGTGCACCAG GATCAGGAAA AACTACTTTA GCCAGGATGT TGTTACAGCA GAACCCATTG 
GGTATTAACC TAAGTACTGA TGAGTATTTT TACAAAAAGG GGCAATACCG ATATGATGTA 
AATCTGTTAG GGGAAGCACA TGAGTGGAAT CATAAACGAG CCAAAGAAGC CTTTGAGAAG 
AATATTTCCC CAATTATAAT AGATAATACC AACTTGCAAG CTTGGGAAAT GAAGCCATAT 
GTGTCTATGG CCATGAAACA CAAGTACAAA GTCACATTTC GTGAACCTGA TACTTGGTGG 
AAGTATAAAC CAAAAGAACT GGAAAGGCGT AACAGTCATG GAGTAACTAA AGAGAAGATC 
AAGAGAATGT TAGAAAATTT TGAACGCGTG ACCGTGAATT CCATTTTAAA TTTATCACGT 
GTAAAGGGGC CAGAACAAAC TGACCTTGGT GAAATAAAAC AAACTGCTGA CAAAAGAGGA 
CAAAACAGTA ATGCCCCAGC CACTCTGAAG AAAGAAGATA TAAGTTCTAC TTCTGAGGAA 
AAAGTAGATG ATAAACGTAT TGCTGATATG GCTGAAGCAT TTTGTAGCCA TTTGGGAGCT 
GAAACTTCAA AAATGTATCC AGACATTTTA CTTAATTCTA CAGATTCAGT GATGAATATT 
CAGACGTCAG GTCATGGTAA AGAGGAACAG TGGGATAGTG TAGTTTCTGA AGAGGACATA 
TATACCAGCC AGAATGCCGA AACCACTGAT GATGATGCCT TTTTACATCA GATGTCTCCT 
GAAAATGAAA TCATCATTAA AGAAAATGTT TCACCTACAG AGGATGCCTT GGTTTTACCT 
GGGCTAATGC CAGAGTTGCT AAACTTTGTT GGAGACTGGC CAGTTGAGCA CACTATGGGT 
CAGAGAGCCC CACGGAGTAG GAGCAAGATT AGAGCAAATT CTACAAGTAA TGAATCATGT 
TCCCTCCAGC TCAGTGAGTT GACAAATGGT GATTCTAAAG AACTGGATAG TCACACTATA 
ACACATGATT TGTTAAATCT AAGCAAAGAA ATCGATAAAT GTACCATTTT ACAAACAGAT 
TATCTTGGAG ATTTGCCAAA TTTGTGTTTT GGGTGTAACG AAGAAAATAT GCAATTAGAA 
GATCAAGTCT GCTCAGCTTT AGAAAGAGAG GCAGAGACGT ACATACCTCA TACAGTACAG 
AACTCTGAAT CAAATCATGT TGAGAAAGAT CCACAAGCAG TCACTGAAGT TACTAAAGAT 
TTGCAAAGCC TTGATATATC TAATATTAAC GAAAATGAAC TTTCAGAATG CGAAACACCA 
GAGAAAAAAA CAAGGCAATG CAAACGAAAT GTTAGACAGT GTAAACTGGC TTTAACTTTT 
ACAAATAGCT GTTCTGACTC TTCCCAAACA GAAGATATAC TGACATATCA ACCAAATGCT 
CCAGTAAATG AAGTCTACTG TGGACCTAGT AAATGTTCGC AAACTGAGCC TCATGAATTT 
GCTTTAGCCT GGAGGATAGA GAAAAAAAAT ATTGACATCT CCAATTCGGC GAAAGTCCTC 
ACTGGCAAAG GTGAACGTTT TCAGTCAAAA TTAATGGATG AAAGTTCAGT TTCACAGGAA 
ACTATTCCCT ATAGAGCAAT GCATCATAAA AGTACATTTG TGGAAGAAGA TGAAATAGTT 
TGCTTGGATG ATGAACATAA TCTAAATATC CTTGCTGGAC TCTTTAGGTC AGTTTCATTT 
GACGTGCTTA TTGATCTTTT TGAAAGATGC AATAAGGACA TTGTGTGGGC ATCCAACTTA 
CTCTTGGATT CCGGGGAGAA AATCTACAAA GATGAAGACT GCCAGACAGA GGATTATTTG 
AGTGTTGTAG GAGATGCCAC TTTGGGAACT GGTCATCGTC CAGACCAAGT TCCTGTATGC 
CCTGATGATC TTAATGCTCC ATCTTTTGGT AGTAGTATGT CACATACTGA TGCTTGTTCC 
AGCACACCAC TAAATGAAAA AAGAGAGCAT GCTGATAGTC ACACAGTTAT AGAAAATACC 
AACCTAACAT ATTCAGAGGC CCTAAGTTCA GAAGATTCAA CAAATCTAAA TGTGTTCTCT 
GTCCCTGATA TAACAAATGA ACAAACCATA GAACAACATA AAATGGTTAC AAAATCATTG 
AATATTGAGC AGCCAAAAGA TACTGATTTG CAACCAGTGT CTAGGATATG CTTATCTGAT 
ATAGATATGG AGGCTTCTCA GAAAACAGCT GATGATATTG ACACAGGAAT CAATGTTTTA 
GAAACACACA ACATTGATAA AGATGTTTGT GAACTTCCCT TGTACGATAT TAATGTACAG 
GACAAAATAC GTGGATTTGC TCTAGAAGAG GTGGATGATG CAAAAAAGAA AAACTCTGAT 
TCCCTCAAAG ACTCTTTGCA TTTTGATCAT CTGGAATTGT GTTTGCCTCC AGAACTAGCC 
TTTCAGCTCA GTGAATTGTT TGGCCCTGTA GGAATAGCTC CAGGTTCTTT AACTATTGAG 
GATTGTACTG TACACATAGA CTTAAATCTG GCCAAAGCTA TCCATACAAG ATGGAAAGAA 
TCTATTACGG AGAGGCAGAA ACAAGAATCC TTGTCTTACC AGCTGCTTTT TGAAGATTTA 
TCGCCTTCAG AACTGGATAA TCTGATAAAT GAAGAGGAAT CTAACTTGGC AAAACCTGAA 
TATTCCGATA CATCGTCTGA CACATTCCCT TTCATGGATC AGTGGAATGT ACGAACCAAG 
AAAGTGTCAT TACGCGAAAT AATGTCAGAG GAAATTGCAT TACAGGAACA AGAATGTCAG 
AAGAAGTCTA CACTAAGGAC AAAAAACTGT GCTGCAAAAC TGAAGGAAAA ACAACTTTTT 
GAATTGTTTC CTAACATTGA ACAAAGGCTT CTTATGGATA TTTTTAAGGA AAATGATTAT 
TCTTTAGAGA GGACTGCAGA GTTCCTAAGT GTCGTTCTGG AAGCTGGCCC AGTGCAGAAT 
ATTGTTGCCT GTGATTTAAA ACAAACAGAT GCCTCTACCT CTGAAAACCC TAAATCAAAG 
AAATTAAATC TGGACAAAAA AACCATCCCT GAGAATTTCT ATCAGGACTT TGAATGCCCA 
GAGTATGATG ATTTCCGGGC TGAAGCTTTC CTTTACCGTA GAAAGCAACA AGAGTGTTAC 
AGGAAAGCAA GCGAGGCTCA CAGCAGAGGC ATGAAACAAG TTGCAACATA TTATGCACAA 
CAGGGGTACC TCTATGGCCA GAAGATGAAA CAGGAAAACC ACAGAGCTGC TGTACAGATC 
TTTGAAAGAA CTAATGAATT CTTGTTGCCA GAGAATATAT TAGACCTGCA CGGGCTCCAT 
GTTAGTGAGG CAATGAAACA ATTGCGGCAG GTACTTCAGA ACAAGATGGA AGATTATAAA 
AAGAATGGTG GCAAATCTTA CCTTTCCGTG ATAACTGGAA GAGGAAATCA TAGCCAAGGA 
GGAGTCCCTC GCATTAAATT AGCAGTCATT GACTATCTCA CAAACCACAG CTTCAGGTTT 
CAGGAGGTAA GGCCGGGAGT GCTGCATATC ACCCTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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n4bp2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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