NKX2-5 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NKX2-5
Entrez Gene ID	396073
Full Name	NK2 homeobox 5
Synonyms	CNKX-2.5,NKX-2.5
General protein information	Preferred Names NK2 homeobox 5 Names homeobox protein Nkx-2.5 NK2 transcription factor related, locus 5 homeobox protein NK-2 homolog E
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205164.1	NP_990495.1	homeobox protein Nkx-2.5

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nkx2-5	NP_032726.1
Homo sapiens (human)	NKX2-5	NP_004378.1
Xenopus tropicalis (tropical clawed frog)	nkx2-5	NP_001116891.1
Bos taurus (cattle)	NKX2-5	NP_001039908.1
Gallus gallus (chicken)	NKX2-5	NP_990495.1
Danio rerio (zebrafish)	nkx2.5	NP_571496.1
Canis lupus familiaris (dog)	NKX2-5	NP_001010959.1
Rattus norvegicus (Norway rat)	Nkx2-5	NP_446103.1
Pan troglodytes (chimpanzee)	NKX2-5	XP_518104.2
	NKX2-5	XP_001096796.1

Gene Ontology
Bibliography

Related articles in PubMed

The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?
Kelder TP, Vicente-Steijn R, Harryvan TJ, Kosmidis G, Gittenberger-de Groot AC, Poelmann RE, Schalij MJ, DeRuiter MC, Jongbloed MR
Journal of cellular and molecular medicine19(6)1375-89(2015 Jun)

Identification and biological characterization of chicken embryonic cardiac progenitor cells.
Bai C, Hou L, Zhang M, Wang L, Guan W, Ma Y
Cell proliferation46(2)232-42(2013 Apr)

Evolutionary conservation of Nkx2.5 autoregulation in the second heart field.
Clark CD, Zhang B, Lee B, Evans SI, Lassar AB, Lee KH
Developmental biology374(1)198-209(2013 Feb)

Differentiation of cardiac Purkinje fibers requires precise spatiotemporal regulation of Nkx2-5 expression.
Harris BS, Spruill L, Edmonson AM, Rackley MS, Benson DW, O'Brien TX, Gourdie RG
Developmental dynamics : an official publication of the American Association of Anatomists235(1)38-49(2006 Jan)

SMAD-mediated modulation of YY1 activity regulates the BMP response and cardiac-specific expression of a GATA4/5/6-dependent chick Nkx2.5 enhancer.
Lee KH, Evans S, Ruan TY, Lassar AB
Development (Cambridge, England)131(19)4709-23(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that myocardial markers ISL1, TNNI2 and NKX2-5 and HCN4 were studied in sequential stages of chick embryo development.
Title: The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?

Nkx2.5 autoregulation is evolutionarily conserved in the second heart field
Title: Evolutionary conservation of Nkx2.5 autoregulation in the second heart field.

Overexpression of Nkx2-5 causes inhibition of slow tonic myosin heavy chain protein, a late Purkinje fiber marker, but does not affect Cx40 levels
Title: Differentiation of cardiac Purkinje fibers requires precise spatiotemporal regulation of Nkx2-5 expression.

characterized the cis-regulatory elements of the tinman homolog Nkx2.5
Title: SMAD-mediated modulation of YY1 activity regulates the BMP response and cardiac-specific expression of a GATA4/5/6-dependent chick Nkx2.5 enhancer.

The following NKX2-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NKX2-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00324	NM_205164.1 Latest version!	Gallus gallus NK2 homeobox 5 (NKX2-5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NKX2-5 ( NM_205164.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990495.1 Gallus gallus NK2 homeobox 5 (NKX2-5), mRNA.

Price & Availability↑

CloneID	OGa00324
Clone ID Related Accession (Same CDS sequence)	NM_205164.1
Accession Version	NM_205164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Gallus gallus(chicken)
Product	homeobox protein Nkx-2.5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X91838.1. ##Evidence-Data-START## Transcript exon combination :: X91838.1 [ECO:0000332] ##Evidence-Data-END##

ATGTTTCCTA GCCCTGTGAC GACGACTCCC TTCTCGGTCA AGGATATTTT GAACCTGGAG 
CAGCAGCAGC AGGGCGGTCT GGCCCCCATG GAGCTCTCTT CGCCCTCCTG CATGTTGGCC 
ACCTTCAAGC AGGAGGCGTT CGGCTCCGAG CCGCCCGCCC TGCCCGAGCT CCCCGAGCCG 
CCCCCGGCCA AGCCGCCCGC CGCCTTCCCC GGCCCCTACT ACGTGAAGAG CTACGGGGAG 
ATGGACACGG CCAAGGATTC CAAGGCGGAC AAGAAAGAAC TGTGTGCCCT GCACAAGAGT 
CTGGAGCAGG AGAAGAGAGA GCTGGAGGAT CCCGAGCGGC CCAGACAGAG GAAGAGGAGG 
AAACCCCGCG TCCTCTTTTC TCAAGCCCAA GTCTACGAAC TGGAGAGAAG GTTCAAGCAG 
CAGAAATACC TCTCAGCTCC CGAGAGAGAC CATCTAGCGA ACGTCCTAAA GCTCACCTCT 
ACCCAGGTGA AAATTTGGTT CCAGAACAGA AGGTACAAAT GCAAAAGGCA GAGACAGGAC 
CAGACCCTGG AGATGGTGGG GATCCCACCC CCCCGGCGGA TAGCGGTGCC GGTGCTGGTG 
CGGGACGGGA AGCCCTGCCT GGGGGAGTCT TCTCCCTACA GCTCGCCCTA CAATGTCAGC 
ATTAACCCCT ATAGCTACAA CGCCTACCCC GCGTACCCTA ACTACAACAG CCCTGCCTGC 
AACGCCAACT ACAACTGCAG CTACCCGGCC GTGCAGCCCG TGCAGCCCTC GGCGGCAGGC 
AACAACTTCA TGAACTTCAG CGTGGGGGAC TTGAATTCGG TGCAGCCGCC CATCCCGCAG 
GGGAACGCGG GCATCTCCAC GTTGCACGGG ATCCGAGCGT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990495.1
CDS	79..963
Translation	MFPSPVTTTPFSVKDILNLEQQQQGGLAPMELSSPSCMLATFKQEAFGSEPPALPELPEPPPAKPPAAFPGPYYVKSYGEMDTAKDSKADKKELCALHKSLEQEKRELEDPERPRQRKRRKPRVLFSQAQVYELERRFKQQKYLSAPERDHLANVLKLTSTQVKIWFQNRRYKCKRQRQDQTLEMVGIPPPRRIAVPVLVRDGKPCLGESSPYSSPYNVSINPYSYNAYPAYPNYNSPACNANYNCSYPAVQPVQPSAAGNNFMNFSVGDLNSVQPPIPQGNAGISTLHGIRAW

Target ORF information:

RefSeq Version	NM_205164.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NK2 homeobox 5 (NKX2-5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205164.1

ORF Insert Sequence:

ATGTTTCCTA GCCCTGTGAC GACGACTCCC TTCTCGGTCA AGGATATTTT GAACCTGGAG 
CAGCAGCAGC AGGGCGGTCT GGCCCCCATG GAGCTCTCTT CGCCCTCCTG CATGTTGGCC 
ACCTTCAAGC AGGAGGCGTT CGGCTCCGAG CCGCCCGCCC TGCCCGAGCT CCCCGAGCCG 
CCCCCGGCCA AGCCGCCCGC CGCCTTCCCC GGCCCCTACT ACGTGAAGAG CTACGGGGAG 
ATGGACACGG CCAAGGATTC CAAGGCGGAC AAGAAAGAAC TGTGTGCCCT GCACAAGAGT 
CTGGAGCAGG AGAAGAGAGA GCTGGAGGAT CCCGAGCGGC CCAGACAGAG GAAGAGGAGG 
AAACCCCGCG TCCTCTTTTC TCAAGCCCAA GTCTACGAAC TGGAGAGAAG GTTCAAGCAG 
CAGAAATACC TCTCAGCTCC CGAGAGAGAC CATCTAGCGA ACGTCCTAAA GCTCACCTCT 
ACCCAGGTGA AAATTTGGTT CCAGAACAGA AGGTACAAAT GCAAAAGGCA GAGACAGGAC 
CAGACCCTGG AGATGGTGGG GATCCCACCC CCCCGGCGGA TAGCGGTGCC GGTGCTGGTG 
CGGGACGGGA AGCCCTGCCT GGGGGAGTCT TCTCCCTACA GCTCGCCCTA CAATGTCAGC 
ATTAACCCCT ATAGCTACAA CGCCTACCCC GCGTACCCTA ACTACAACAG CCCTGCCTGC 
AACGCCAACT ACAACTGCAG CTACCCGGCC GTGCAGCCCG TGCAGCCCTC GGCGGCAGGC 
AACAACTTCA TGAACTTCAG CGTGGGGGAC TTGAATTCGG TGCAGCCGCC CATCCCGCAG 
GGGAACGCGG GCATCTCCAC GTTGCACGGG ATCCGAGCGT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?
Journal of cellular and molecular medicine19(6)1375-89(2015 Jun)
Kelder TP,Vicente-Steijn R,Harryvan TJ,Kosmidis G,Gittenberger-de Groot AC,Poelmann RE,Schalij MJ,DeRuiter MC,Jongbloed MR

Identification and biological characterization of chicken embryonic cardiac progenitor cells.
Cell proliferation46(2)232-42(2013 Apr)
Bai C,Hou L,Zhang M,Wang L,Guan W,Ma Y

Evolutionary conservation of Nkx2.5 autoregulation in the second heart field.
Developmental biology374(1)198-209(2013 Feb)
Clark CD,Zhang B,Lee B,Evans SI,Lassar AB,Lee KH

Differentiation of cardiac Purkinje fibers requires precise spatiotemporal regulation of Nkx2-5 expression.
Developmental dynamics : an official publication of the American Association of Anatomists235(1)38-49(2006 Jan)
Harris BS,Spruill L,Edmonson AM,Rackley MS,Benson DW,O'Brien TX,Gourdie RG

SMAD-mediated modulation of YY1 activity regulates the BMP response and cardiac-specific expression of a GATA4/5/6-dependent chick Nkx2.5 enhancer.
Development (Cambridge, England)131(19)4709-23(2004 Oct)
Lee KH,Evans S,Ruan TY,Lassar AB

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NKX2-5 cDNA ORF clone, Gallus gallus(chicken)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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