MYLK cDNA ORF clone, Canis lupus familiaris(Dog)

  • Gene

  • Clones

  • gRNAs

The following MYLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe44726 XM_014110236.2
Latest version!
Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe44727 XM_014110237.2
Latest version!
Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe284276 XM_022413886.1
Latest version!
Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe284277 XM_022413887.1
Latest version!
Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe44723 XM_014110233.2
Latest version!
Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe44724 XM_014110234.2
Latest version!
Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe44725 XM_014110235.2
Latest version!
Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OCe44723 XM_014110233.1 Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe44724 XM_014110234.1 Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe44726 XM_014110236.1 Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe44725 XM_014110235.1 Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe44727 XM_014110237.1 Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe44726
    Clone ID Related Accession (Same CDS sequence) XM_014110236.1 , XM_014110236.2
    Accession Version XM_014110236.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product myosin light chain kinase, smooth muscle isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726118.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAAGCCC GGAGGCCCAA GAGCTCTCTA CCTCCCATGC TAGGAACAGA GAGTGATGCA 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCAAAAG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CTGAGGACCA AAAGGTGCGC GCAGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCAGGCA CCCAGCCCAT CACCTGTACG TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGCGAGCACA TCAAGGTGGA GACCAGTGAG AATGGCAGCA GGCTCACCAT CGTGGCCGCA
    CGCCAGGAAC ACTGCGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGACAAA CCAGACCCCC CAGCGGGCAC ACCTTGCGCC
    TCCGACATCC GGAGCTCGTC GTTGACCCTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGTGCCGTGC AATCCTACAG CGTCGAGATC TGGGACTCGG TGGACAAGAC GTGGAAGGAA
    CTAGCCACGT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGCCTGA CCGAGAGTAT
    AAATTCCGCG TTCGCGCCAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAGCAG TGGGAGAGAA ACCGGAGGAG CCGAAGGACG AAGTGGAGGT GTCGGATGAT
    GATGAGAAGG AACCTGAGGT CGACTACCGG ACAGTGACGG TCAACACTGA ACAAAAAGTG
    TCCGACTTCT ATGACATCGA AGAGAGACTA GGCTCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAAA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC ATATTCAGCC
    AAAGAGAAGG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGATGC CTTCGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCGGGGG GCGAGCTGTT CGAGCGCATC ATCGACGAGG ACTTCGAGCT GACGGAGCGC
    GAGTGCATCC AGTACATGAG GCAGATCTCG GCGGGCGTGG AGTACATCCA CCGGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCCGAGAAC ATCATGTGCG TCAATAAGAC CGGCACGAGG
    ATCAAGCTCA TCGACTTCGG CCTGGCCAGA AAGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGTA CCCCGGAATT TGTGGCCCCC GAAGTGATCA ACTATGAGCC CATCGGCTAT
    GCCACGGACA TGTGGAGCAT CGGAGTCATC TGCTACATCC TGGTTAGTGG ACTCTCCCCC
    TTCATGGGTG ACAACGATAA TGAAACCCTA GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GATGATGAGG CGTTCGATGA GATCTCGGAT GACGCCAAGG ATTTTATCAG CAATTTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGT ACCCAGTGCC TTCAGCATCC CTGGCTCATG
    AAAGACACCA AGAACATGGA AGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATCGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGATCACC GACCAGCCCG
    CTCAACGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCCCATGT AAAACCCTAT TTCTCTAAGA CCATTCGTGA TTTAGAAGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAAGTT
    GTCTGGTTTA AAGATGACCA GTCAATCCGG GAGTCCCGCC ACTTCCAGAT AGACTACGAC
    GAGGATGGGA ACTGCTCTTT AATCATTAGC GATGTTTGTG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAATAG TCTTGGAGAA GCCACTTGCA CAGCAGAGCT CATTGTGGAG
    ACCATGGAGG AAGGGGAAGG GGAAGGGGAA GAGGAAGAAG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965711.1
    CDS127..2277
    Translation

    Target ORF information:

    RefSeq Version XM_014110236.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAAGCCC GGAGGCCCAA GAGCTCTCTA CCTCCCATGC TAGGAACAGA GAGTGATGCA 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCAAAAG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CTGAGGACCA AAAGGTGCGC GCAGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCAGGCA CCCAGCCCAT CACCTGTACG TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGCGAGCACA TCAAGGTGGA GACCAGTGAG AATGGCAGCA GGCTCACCAT CGTGGCCGCA
    CGCCAGGAAC ACTGCGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGACAAA CCAGACCCCC CAGCGGGCAC ACCTTGCGCC
    TCCGACATCC GGAGCTCGTC GTTGACCCTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGTGCCGTGC AATCCTACAG CGTCGAGATC TGGGACTCGG TGGACAAGAC GTGGAAGGAA
    CTAGCCACGT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGCCTGA CCGAGAGTAT
    AAATTCCGCG TTCGCGCCAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAGCAG TGGGAGAGAA ACCGGAGGAG CCGAAGGACG AAGTGGAGGT GTCGGATGAT
    GATGAGAAGG AACCTGAGGT CGACTACCGG ACAGTGACGG TCAACACTGA ACAAAAAGTG
    TCCGACTTCT ATGACATCGA AGAGAGACTA GGCTCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAAA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC ATATTCAGCC
    AAAGAGAAGG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGATGC CTTCGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCGGGGG GCGAGCTGTT CGAGCGCATC ATCGACGAGG ACTTCGAGCT GACGGAGCGC
    GAGTGCATCC AGTACATGAG GCAGATCTCG GCGGGCGTGG AGTACATCCA CCGGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCCGAGAAC ATCATGTGCG TCAATAAGAC CGGCACGAGG
    ATCAAGCTCA TCGACTTCGG CCTGGCCAGA AAGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGTA CCCCGGAATT TGTGGCCCCC GAAGTGATCA ACTATGAGCC CATCGGCTAT
    GCCACGGACA TGTGGAGCAT CGGAGTCATC TGCTACATCC TGGTTAGTGG ACTCTCCCCC
    TTCATGGGTG ACAACGATAA TGAAACCCTA GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GATGATGAGG CGTTCGATGA GATCTCGGAT GACGCCAAGG ATTTTATCAG CAATTTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGT ACCCAGTGCC TTCAGCATCC CTGGCTCATG
    AAAGACACCA AGAACATGGA AGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATCGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGATCACC GACCAGCCCG
    CTCAACGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCCCATGT AAAACCCTAT TTCTCTAAGA CCATTCGTGA TTTAGAAGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAAGTT
    GTCTGGTTTA AAGATGACCA GTCAATCCGG GAGTCCCGCC ACTTCCAGAT AGACTACGAC
    GAGGATGGGA ACTGCTCTTT AATCATTAGC GATGTTTGTG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAATAG TCTTGGAGAA GCCACTTGCA CAGCAGAGCT CATTGTGGAG
    ACCATGGAGG AAGGGGAAGG GGAAGGGGAA GAGGAAGAAG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44726
    Clone ID Related Accession (Same CDS sequence) XM_014110236.1 , XM_014110236.2
    Accession Version XM_014110236.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myosin light chain kinase, smooth muscle isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAAGCCC GGAGGCCCAA GAGCTCTCTA CCTCCCATGC TAGGAACAGA GAGTGATGCA 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCAAAAG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CTGAGGACCA AAAGGTGCGC GCAGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCAGGCA CCCAGCCCAT CACCTGTACG TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGCGAGCACA TCAAGGTGGA GACCAGTGAG AATGGCAGCA GGCTCACCAT CGTGGCCGCA
    CGCCAGGAAC ACTGCGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGACAAA CCAGACCCCC CAGCGGGCAC ACCTTGCGCC
    TCCGACATCC GGAGCTCGTC GTTGACCCTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGTGCCGTGC AATCCTACAG CGTCGAGATC TGGGACTCGG TGGACAAGAC GTGGAAGGAA
    CTAGCCACGT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGCCTGA CCGAGAGTAT
    AAATTCCGCG TTCGCGCCAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAGCAG TGGGAGAGAA ACCGGAGGAG CCGAAGGACG AAGTGGAGGT GTCGGATGAT
    GATGAGAAGG AACCTGAGGT CGACTACCGG ACAGTGACGG TCAACACTGA ACAAAAAGTG
    TCCGACTTCT ATGACATCGA AGAGAGACTA GGCTCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAAA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC ATATTCAGCC
    AAAGAGAAGG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGATGC CTTCGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCGGGGG GCGAGCTGTT CGAGCGCATC ATCGACGAGG ACTTCGAGCT GACGGAGCGC
    GAGTGCATCC AGTACATGAG GCAGATCTCG GCGGGCGTGG AGTACATCCA CCGGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCCGAGAAC ATCATGTGCG TCAATAAGAC CGGCACGAGG
    ATCAAGCTCA TCGACTTCGG CCTGGCCAGA AAGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGTA CCCCGGAATT TGTGGCCCCC GAAGTGATCA ACTATGAGCC CATCGGCTAT
    GCCACGGACA TGTGGAGCAT CGGAGTCATC TGCTACATCC TGGTTAGTGG ACTCTCCCCC
    TTCATGGGTG ACAACGATAA TGAAACCCTA GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GATGATGAGG CGTTCGATGA GATCTCGGAT GACGCCAAGG ATTTTATCAG CAATTTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGT ACCCAGTGCC TTCAGCATCC CTGGCTCATG
    AAAGACACCA AGAACATGGA AGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATCGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGATCACC GACCAGCCCG
    CTCAACGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCCCATGT AAAACCCTAT TTCTCTAAGA CCATTCGTGA TTTAGAAGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAAGTT
    GTCTGGTTTA AAGATGACCA GTCAATCCGG GAGTCCCGCC ACTTCCAGAT AGACTACGAC
    GAGGATGGGA ACTGCTCTTT AATCATTAGC GATGTTTGTG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAATAG TCTTGGAGAA GCCACTTGCA CAGCAGAGCT CATTGTGGAG
    ACCATGGAGG AAGGGGAAGG GGAAGGGGAA GAGGAAGAAG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965711.1
    CDS178..2328
    Translation

    Target ORF information:

    RefSeq Version XM_014110236.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110236.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAAGCCC GGAGGCCCAA GAGCTCTCTA CCTCCCATGC TAGGAACAGA GAGTGATGCA 
    ACTGTGAAAA AGAAACCTGC CCCCAAGACA CCTCCAAAAG CAGCCATGCC CCCTCAGATC
    ATCCAGTTTC CTGAGGACCA AAAGGTGCGC GCAGGAGAGT CTGTGGAGCT GTTTGGTAAA
    GTGGCAGGCA CCCAGCCCAT CACCTGTACG TGGATGAAGT TCCGAAAGCA GATCCAGGAG
    AGCGAGCACA TCAAGGTGGA GACCAGTGAG AATGGCAGCA GGCTCACCAT CGTGGCCGCA
    CGCCAGGAAC ACTGCGGCTG CTACACTCTG CTGGTGGAGA ACAAGCTGGG CAGCAGGCAG
    GCCCAGGTCA ACCTCACTGT CGTGGACAAA CCAGACCCCC CAGCGGGCAC ACCTTGCGCC
    TCCGACATCC GGAGCTCGTC GTTGACCCTG TCCTGGTATG GCTCCTCGTA TGATGGGGGC
    AGTGCCGTGC AATCCTACAG CGTCGAGATC TGGGACTCGG TGGACAAGAC GTGGAAGGAA
    CTAGCCACGT GCCGCAGCAC CTCTTTTAAC GTCCAGGACC TGCTGCCTGA CCGAGAGTAT
    AAATTCCGCG TTCGCGCCAT CAACGTGTAT GGAACCAGTG AGCCAAGCCA GGAGTCTGAA
    CTCACAGCAG TGGGAGAGAA ACCGGAGGAG CCGAAGGACG AAGTGGAGGT GTCGGATGAT
    GATGAGAAGG AACCTGAGGT CGACTACCGG ACAGTGACGG TCAACACTGA ACAAAAAGTG
    TCCGACTTCT ATGACATCGA AGAGAGACTA GGCTCTGGGA AATTTGGACA GGTCTTCCGA
    CTTGTAGAAA AGAAAACTGG AAAAATCTGG GCAGGGAAAT TCTTCAAGGC ATATTCAGCC
    AAAGAGAAGG AGAATATCCG GCAGGAGATT AGCATCATGA ACTGCCTCCA CCACCCCAAG
    CTGGTCCAGT GTGTGGATGC CTTCGAAGAA AAGGCCAACA TCGTCATGGT CCTGGAGATC
    GTGTCGGGGG GCGAGCTGTT CGAGCGCATC ATCGACGAGG ACTTCGAGCT GACGGAGCGC
    GAGTGCATCC AGTACATGAG GCAGATCTCG GCGGGCGTGG AGTACATCCA CCGGCAGGGC
    ATCGTCCACC TGGACCTCAA GCCCGAGAAC ATCATGTGCG TCAATAAGAC CGGCACGAGG
    ATCAAGCTCA TCGACTTCGG CCTGGCCAGA AAGCTGGAGA ATGCGGGGTC TCTGAAGGTC
    CTCTTTGGTA CCCCGGAATT TGTGGCCCCC GAAGTGATCA ACTATGAGCC CATCGGCTAT
    GCCACGGACA TGTGGAGCAT CGGAGTCATC TGCTACATCC TGGTTAGTGG ACTCTCCCCC
    TTCATGGGTG ACAACGATAA TGAAACCCTA GCCAACGTTA CCTCAGCCAC CTGGGACTTC
    GATGATGAGG CGTTCGATGA GATCTCGGAT GACGCCAAGG ATTTTATCAG CAATTTGCTG
    AAGAAAGATA TGAAAAACCG CCTGGACTGT ACCCAGTGCC TTCAGCATCC CTGGCTCATG
    AAAGACACCA AGAACATGGA AGCCAAGAAA CTCTCCAAGG ACCGGATGAA GAAGTACATG
    GCAAGAAGGA AATGGCAGAA AACGGGCAAT GCTGTGAGAG CCATCGGAAG ACTGTCCTCT
    ATGGCAATGA TCTCAGGGCT CAGTGGCAGG AAATCCTCAA CAGGATCACC GACCAGCCCG
    CTCAACGCAG AAAAACTAGA ATCTGAAGAA GATGTGTCCC AAGCTTTCCT TGAGGCTGTT
    GCTGAGGAAA AGCCCCATGT AAAACCCTAT TTCTCTAAGA CCATTCGTGA TTTAGAAGTT
    GTGGAGGGAA GCGCTGCTAG ATTTGACTGC AAGATTGAAG GATACCCAGA CCCTGAAGTT
    GTCTGGTTTA AAGATGACCA GTCAATCCGG GAGTCCCGCC ACTTCCAGAT AGACTACGAC
    GAGGATGGGA ACTGCTCTTT AATCATTAGC GATGTTTGTG GGGATGACGA TGCCAAGTAC
    ACCTGCAAGG CTGTCAATAG TCTTGGAGAA GCCACTTGCA CAGCAGAGCT CATTGTGGAG
    ACCATGGAGG AAGGGGAAGG GGAAGGGGAA GAGGAAGAAG AGGAAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44727
    Clone ID Related Accession (Same CDS sequence) XM_014110237.2 , XM_014110237.1
    Accession Version XM_014110237.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myosin light chain kinase, smooth muscle isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCCCCCTC AGATCATCCA GTTTCCTGAG GACCAAAAGG TGCGCGCAGG AGAGTCTGTG 
    GAGCTGTTTG GTAAAGTGGC AGGCACCCAG CCCATCACCT GTACGTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGCGA GCACATCAAG GTGGAGACCA GTGAGAATGG CAGCAGGCTC
    ACCATCGTGG CCGCACGCCA GGAACACTGC GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTCGTGG ACAAACCAGA CCCCCCAGCG
    GGCACACCTT GCGCCTCCGA CATCCGGAGC TCGTCGTTGA CCCTGTCCTG GTATGGCTCC
    TCGTATGATG GGGGCAGTGC CGTGCAATCC TACAGCGTCG AGATCTGGGA CTCGGTGGAC
    AAGACGTGGA AGGAACTAGC CACGTGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    CCTGACCGAG AGTATAAATT CCGCGTTCGC GCCATCAACG TGTATGGAAC CAGTGAGCCA
    AGCCAGGAGT CTGAACTCAC AGCAGTGGGA GAGAAACCGG AGGAGCCGAA GGACGAAGTG
    GAGGTGTCGG ATGATGATGA GAAGGAACCT GAGGTCGACT ACCGGACAGT GACGGTCAAC
    ACTGAACAAA AAGTGTCCGA CTTCTATGAC ATCGAAGAGA GACTAGGCTC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAAAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCATATT CAGCCAAAGA GAAGGAGAAT ATCCGGCAGG AGATTAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GATGCCTTCG AAGAAAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC GGGGGGCGAG CTGTTCGAGC GCATCATCGA CGAGGACTTC
    GAGCTGACGG AGCGCGAGTG CATCCAGTAC ATGAGGCAGA TCTCGGCGGG CGTGGAGTAC
    ATCCACCGGC AGGGCATCGT CCACCTGGAC CTCAAGCCCG AGAACATCAT GTGCGTCAAT
    AAGACCGGCA CGAGGATCAA GCTCATCGAC TTCGGCCTGG CCAGAAAGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGTACCCCG GAATTTGTGG CCCCCGAAGT GATCAACTAT
    GAGCCCATCG GCTATGCCAC GGACATGTGG AGCATCGGAG TCATCTGCTA CATCCTGGTT
    AGTGGACTCT CCCCCTTCAT GGGTGACAAC GATAATGAAA CCCTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGATGA TGAGGCGTTC GATGAGATCT CGGATGACGC CAAGGATTTT
    ATCAGCAATT TGCTGAAGAA AGATATGAAA AACCGCCTGG ACTGTACCCA GTGCCTTCAG
    CATCCCTGGC TCATGAAAGA CACCAAGAAC ATGGAAGCCA AGAAACTCTC CAAGGACCGG
    ATGAAGAAGT ACATGGCAAG AAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATC
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGA
    TCACCGACCA GCCCGCTCAA CGCAGAAAAA CTAGAATCTG AAGAAGATGT GTCCCAAGCT
    TTCCTTGAGG CTGTTGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTCTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AAGTTGTCTG GTTTAAAGAT GACCAGTCAA TCCGGGAGTC CCGCCACTTC
    CAGATAGACT ACGACGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT TTGTGGGGAT
    GACGATGCCA AGTACACCTG CAAGGCTGTC AATAGTCTTG GAGAAGCCAC TTGCACAGCA
    GAGCTCATTG TGGAGACCAT GGAGGAAGGG GAAGGGGAAG GGGAAGAGGA AGAAGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965712.1
    CDS243..2288
    Translation

    Target ORF information:

    RefSeq Version XM_014110237.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110237.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCCCCCTC AGATCATCCA GTTTCCTGAG GACCAAAAGG TGCGCGCAGG AGAGTCTGTG 
    GAGCTGTTTG GTAAAGTGGC AGGCACCCAG CCCATCACCT GTACGTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGCGA GCACATCAAG GTGGAGACCA GTGAGAATGG CAGCAGGCTC
    ACCATCGTGG CCGCACGCCA GGAACACTGC GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTCGTGG ACAAACCAGA CCCCCCAGCG
    GGCACACCTT GCGCCTCCGA CATCCGGAGC TCGTCGTTGA CCCTGTCCTG GTATGGCTCC
    TCGTATGATG GGGGCAGTGC CGTGCAATCC TACAGCGTCG AGATCTGGGA CTCGGTGGAC
    AAGACGTGGA AGGAACTAGC CACGTGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    CCTGACCGAG AGTATAAATT CCGCGTTCGC GCCATCAACG TGTATGGAAC CAGTGAGCCA
    AGCCAGGAGT CTGAACTCAC AGCAGTGGGA GAGAAACCGG AGGAGCCGAA GGACGAAGTG
    GAGGTGTCGG ATGATGATGA GAAGGAACCT GAGGTCGACT ACCGGACAGT GACGGTCAAC
    ACTGAACAAA AAGTGTCCGA CTTCTATGAC ATCGAAGAGA GACTAGGCTC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAAAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCATATT CAGCCAAAGA GAAGGAGAAT ATCCGGCAGG AGATTAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GATGCCTTCG AAGAAAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC GGGGGGCGAG CTGTTCGAGC GCATCATCGA CGAGGACTTC
    GAGCTGACGG AGCGCGAGTG CATCCAGTAC ATGAGGCAGA TCTCGGCGGG CGTGGAGTAC
    ATCCACCGGC AGGGCATCGT CCACCTGGAC CTCAAGCCCG AGAACATCAT GTGCGTCAAT
    AAGACCGGCA CGAGGATCAA GCTCATCGAC TTCGGCCTGG CCAGAAAGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGTACCCCG GAATTTGTGG CCCCCGAAGT GATCAACTAT
    GAGCCCATCG GCTATGCCAC GGACATGTGG AGCATCGGAG TCATCTGCTA CATCCTGGTT
    AGTGGACTCT CCCCCTTCAT GGGTGACAAC GATAATGAAA CCCTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGATGA TGAGGCGTTC GATGAGATCT CGGATGACGC CAAGGATTTT
    ATCAGCAATT TGCTGAAGAA AGATATGAAA AACCGCCTGG ACTGTACCCA GTGCCTTCAG
    CATCCCTGGC TCATGAAAGA CACCAAGAAC ATGGAAGCCA AGAAACTCTC CAAGGACCGG
    ATGAAGAAGT ACATGGCAAG AAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATC
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGA
    TCACCGACCA GCCCGCTCAA CGCAGAAAAA CTAGAATCTG AAGAAGATGT GTCCCAAGCT
    TTCCTTGAGG CTGTTGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTCTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AAGTTGTCTG GTTTAAAGAT GACCAGTCAA TCCGGGAGTC CCGCCACTTC
    CAGATAGACT ACGACGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT TTGTGGGGAT
    GACGATGCCA AGTACACCTG CAAGGCTGTC AATAGTCTTG GAGAAGCCAC TTGCACAGCA
    GAGCTCATTG TGGAGACCAT GGAGGAAGGG GAAGGGGAAG GGGAAGAGGA AGAAGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44727
    Clone ID Related Accession (Same CDS sequence) XM_014110237.2 , XM_014110237.1
    Accession Version XM_014110237.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product myosin light chain kinase, smooth muscle isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726118.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCCCCCTC AGATCATCCA GTTTCCTGAG GACCAAAAGG TGCGCGCAGG AGAGTCTGTG 
    GAGCTGTTTG GTAAAGTGGC AGGCACCCAG CCCATCACCT GTACGTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGCGA GCACATCAAG GTGGAGACCA GTGAGAATGG CAGCAGGCTC
    ACCATCGTGG CCGCACGCCA GGAACACTGC GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTCGTGG ACAAACCAGA CCCCCCAGCG
    GGCACACCTT GCGCCTCCGA CATCCGGAGC TCGTCGTTGA CCCTGTCCTG GTATGGCTCC
    TCGTATGATG GGGGCAGTGC CGTGCAATCC TACAGCGTCG AGATCTGGGA CTCGGTGGAC
    AAGACGTGGA AGGAACTAGC CACGTGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    CCTGACCGAG AGTATAAATT CCGCGTTCGC GCCATCAACG TGTATGGAAC CAGTGAGCCA
    AGCCAGGAGT CTGAACTCAC AGCAGTGGGA GAGAAACCGG AGGAGCCGAA GGACGAAGTG
    GAGGTGTCGG ATGATGATGA GAAGGAACCT GAGGTCGACT ACCGGACAGT GACGGTCAAC
    ACTGAACAAA AAGTGTCCGA CTTCTATGAC ATCGAAGAGA GACTAGGCTC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAAAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCATATT CAGCCAAAGA GAAGGAGAAT ATCCGGCAGG AGATTAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GATGCCTTCG AAGAAAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC GGGGGGCGAG CTGTTCGAGC GCATCATCGA CGAGGACTTC
    GAGCTGACGG AGCGCGAGTG CATCCAGTAC ATGAGGCAGA TCTCGGCGGG CGTGGAGTAC
    ATCCACCGGC AGGGCATCGT CCACCTGGAC CTCAAGCCCG AGAACATCAT GTGCGTCAAT
    AAGACCGGCA CGAGGATCAA GCTCATCGAC TTCGGCCTGG CCAGAAAGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGTACCCCG GAATTTGTGG CCCCCGAAGT GATCAACTAT
    GAGCCCATCG GCTATGCCAC GGACATGTGG AGCATCGGAG TCATCTGCTA CATCCTGGTT
    AGTGGACTCT CCCCCTTCAT GGGTGACAAC GATAATGAAA CCCTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGATGA TGAGGCGTTC GATGAGATCT CGGATGACGC CAAGGATTTT
    ATCAGCAATT TGCTGAAGAA AGATATGAAA AACCGCCTGG ACTGTACCCA GTGCCTTCAG
    CATCCCTGGC TCATGAAAGA CACCAAGAAC ATGGAAGCCA AGAAACTCTC CAAGGACCGG
    ATGAAGAAGT ACATGGCAAG AAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATC
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGA
    TCACCGACCA GCCCGCTCAA CGCAGAAAAA CTAGAATCTG AAGAAGATGT GTCCCAAGCT
    TTCCTTGAGG CTGTTGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTCTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AAGTTGTCTG GTTTAAAGAT GACCAGTCAA TCCGGGAGTC CCGCCACTTC
    CAGATAGACT ACGACGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT TTGTGGGGAT
    GACGATGCCA AGTACACCTG CAAGGCTGTC AATAGTCTTG GAGAAGCCAC TTGCACAGCA
    GAGCTCATTG TGGAGACCAT GGAGGAAGGG GAAGGGGAAG GGGAAGAGGA AGAAGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965712.1
    CDS285..2330
    Translation

    Target ORF information:

    RefSeq Version XM_014110237.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCCCCCTC AGATCATCCA GTTTCCTGAG GACCAAAAGG TGCGCGCAGG AGAGTCTGTG 
    GAGCTGTTTG GTAAAGTGGC AGGCACCCAG CCCATCACCT GTACGTGGAT GAAGTTCCGA
    AAGCAGATCC AGGAGAGCGA GCACATCAAG GTGGAGACCA GTGAGAATGG CAGCAGGCTC
    ACCATCGTGG CCGCACGCCA GGAACACTGC GGCTGCTACA CTCTGCTGGT GGAGAACAAG
    CTGGGCAGCA GGCAGGCCCA GGTCAACCTC ACTGTCGTGG ACAAACCAGA CCCCCCAGCG
    GGCACACCTT GCGCCTCCGA CATCCGGAGC TCGTCGTTGA CCCTGTCCTG GTATGGCTCC
    TCGTATGATG GGGGCAGTGC CGTGCAATCC TACAGCGTCG AGATCTGGGA CTCGGTGGAC
    AAGACGTGGA AGGAACTAGC CACGTGCCGC AGCACCTCTT TTAACGTCCA GGACCTGCTG
    CCTGACCGAG AGTATAAATT CCGCGTTCGC GCCATCAACG TGTATGGAAC CAGTGAGCCA
    AGCCAGGAGT CTGAACTCAC AGCAGTGGGA GAGAAACCGG AGGAGCCGAA GGACGAAGTG
    GAGGTGTCGG ATGATGATGA GAAGGAACCT GAGGTCGACT ACCGGACAGT GACGGTCAAC
    ACTGAACAAA AAGTGTCCGA CTTCTATGAC ATCGAAGAGA GACTAGGCTC TGGGAAATTT
    GGACAGGTCT TCCGACTTGT AGAAAAGAAA ACTGGAAAAA TCTGGGCAGG GAAATTCTTC
    AAGGCATATT CAGCCAAAGA GAAGGAGAAT ATCCGGCAGG AGATTAGCAT CATGAACTGC
    CTCCACCACC CCAAGCTGGT CCAGTGTGTG GATGCCTTCG AAGAAAAGGC CAACATCGTC
    ATGGTCCTGG AGATCGTGTC GGGGGGCGAG CTGTTCGAGC GCATCATCGA CGAGGACTTC
    GAGCTGACGG AGCGCGAGTG CATCCAGTAC ATGAGGCAGA TCTCGGCGGG CGTGGAGTAC
    ATCCACCGGC AGGGCATCGT CCACCTGGAC CTCAAGCCCG AGAACATCAT GTGCGTCAAT
    AAGACCGGCA CGAGGATCAA GCTCATCGAC TTCGGCCTGG CCAGAAAGCT GGAGAATGCG
    GGGTCTCTGA AGGTCCTCTT TGGTACCCCG GAATTTGTGG CCCCCGAAGT GATCAACTAT
    GAGCCCATCG GCTATGCCAC GGACATGTGG AGCATCGGAG TCATCTGCTA CATCCTGGTT
    AGTGGACTCT CCCCCTTCAT GGGTGACAAC GATAATGAAA CCCTAGCCAA CGTTACCTCA
    GCCACCTGGG ACTTCGATGA TGAGGCGTTC GATGAGATCT CGGATGACGC CAAGGATTTT
    ATCAGCAATT TGCTGAAGAA AGATATGAAA AACCGCCTGG ACTGTACCCA GTGCCTTCAG
    CATCCCTGGC TCATGAAAGA CACCAAGAAC ATGGAAGCCA AGAAACTCTC CAAGGACCGG
    ATGAAGAAGT ACATGGCAAG AAGGAAATGG CAGAAAACGG GCAATGCTGT GAGAGCCATC
    GGAAGACTGT CCTCTATGGC AATGATCTCA GGGCTCAGTG GCAGGAAATC CTCAACAGGA
    TCACCGACCA GCCCGCTCAA CGCAGAAAAA CTAGAATCTG AAGAAGATGT GTCCCAAGCT
    TTCCTTGAGG CTGTTGCTGA GGAAAAGCCC CATGTAAAAC CCTATTTCTC TAAGACCATT
    CGTGATTTAG AAGTTGTGGA GGGAAGCGCT GCTAGATTTG ACTGCAAGAT TGAAGGATAC
    CCAGACCCTG AAGTTGTCTG GTTTAAAGAT GACCAGTCAA TCCGGGAGTC CCGCCACTTC
    CAGATAGACT ACGACGAGGA TGGGAACTGC TCTTTAATCA TTAGCGATGT TTGTGGGGAT
    GACGATGCCA AGTACACCTG CAAGGCTGTC AATAGTCTTG GAGAAGCCAC TTGCACAGCA
    GAGCTCATTG TGGAGACCAT GGAGGAAGGG GAAGGGGAAG GGGAAGAGGA AGAAGAGGAA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284276
    Clone ID Related Accession (Same CDS sequence) XM_022413886.1
    Accession Version XM_022413886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myosin light chain kinase, smooth muscle isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGATACATC TGACGATCCA GAAGGTCCGG GGTTACCCAG AGCCCCAGGT GACATGGCAC 
    CGAAATGGGC AACCCATCAC CAGTGGGGGC CGCTTCCTGC TGGATTGTGG TATCCGGGGG
    ACTTTCAGCC TCGTGATTCA TGCTGTCCGT GAGGAGGACC AGGGAAAGTA TACCTGCGAA
    GCCACCAATG ATGGTGGTGC CCGCCAGGTG ACAGTGGAGT TGACTGTAGA AGGGGGCTTC
    ATGAAGAAGC ATGGTCAGCC CGTTGTTTCC AAAACCTTGG GGGACAGATT TGCAGCCCCC
    GCAGTGGAGA CCCGTCCTAG CATCTGGGGA GAGTGCCCAC CAAAGTTTGC TACCAAGTTG
    GGCCGAGCCG TAGTCAAAGA AGGACAGACG GGACGGTTCT CCTGCAAGAT CACTGGACGG
    CCCCAACCAC AGGTCACCTG GCTCAAGGGG GATGTTGCCC TGCAGCCAAG TGCCCGTGTG
    TCTATGTCTG AGAAGAACGG GGTACAAGTT CTGGAAATCC ATGAGGTCAG CCGCGATGAT
    GTGGGAGTGT ACACATGCAT GGTGGTGAAT GGGTCGGGGA AGGCCTCCAT GTCGGCTGAG
    CTTTCTATCC AAGGTTTGGA CAATACCAGT AGGTCATTAA CAAAGGTGGC CAATTCAGAT
    ATCAGGAAGG AGGTGACCAA TGGAACCACG CAGGGGCCGA AACTAGACCA CCTGGAGTCT
    GCAGCGGAAA GGTGGCCCTG CTCCAGCACC CAGATCGGTG GCTCCCCCAC CTGGGCCACA
    AGTAGCCAGC CTCAGCCCCT GAGGGAGTCC AAGCTGGAGC CGCCTGGGGA CCCACCCAGG
    AAAGCCCTGA GGAGCCCCGT CCTACAGAAG ACTTCCAGCA CCATTACCCT GCAGGCGGCA
    AAAGTCCAGC CAGAGCCAAG AGCGCCGGTC TCAAGTGCCC TCTCGTCTCC CGGAGAAGAG
    AGGGAGAGGC CAGCTGCTCC CCCCACAGCC ACCTTCCCTG CCCGGCAGTC TGGCCTGGGA
    AGCCAGGAGG TTGTGAACAA GGTTGCTACC AGAAAAATCC CCATGGAAAG CCAGAGAGAT
    TCCACATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAGTGA AGATGAAACA
    GTCAAGTTCA GATGTGAGGT TTCAGGGACC CCAAAGCCTG AAGTGACCTG GTTCCTGGAA
    GGTGCCCCCG TGAAGAGACG AGAAGGCATG GTTGAGGTTT ATGAAGAAGG TGGATGCCAC
    TACCTCTGCT TGCTGAGAGT GCGGGCCAAG GACAGTGGGA GCTACAGTTG CACGGCCTCC
    AATGTCCGAG GCCAGGTGTC CTGTGGTTGG ACCCTCCTGG TGAAACGGCT GGCCGTGATG
    GAGGTGGCTC CCTCGTTCTC CAGCGTCCTG AAGGACTGCG CTGTCATTGA GGGCCAGGAC
    TTTGTGCTGC AGTGCTCAGT GCAGGGGACC CCGGTGCCCC AAATCACTTG GCTGCTTAAT
    GAGCAGCCCA TCCAGTACGC TCACTCCTCC TGCGAGGCTG GCGTGGCCGA GCTCCACATC
    CAGGATGCCC TGCCGGAAGA TGATGGCATT TACACCTGTC TGGCCAAGAA CACCTTGGGC
    CAGGTGTCCT GCAGCGCCCG GGTCACCGTC CATGAAAAGA AGAGCGACAG GAAGAGTGGG
    TATCTTCTGC GCACAGCTCC CGGCAAGCCC GTCGCACCCC TCTTCCTGCA GGGCCTCTCT
    GACCTCAAAG TCATGGATGG AAGCCAGGTC ACCATGACAG TCCAGGTGTC AGGGAATCCG
    CCCCCCGAGG TCATCTGGCT TCACAACGGG AATGAGATCC AGGAGTCAGA GGACTTCCAC
    TTTGAACAGA GAGGCACTCA GCACAGCCTT TGTATCCAGG AAGTGTTCCC AGAGGACACA
    GGCACTTACA CCTGCGAAGC CTGGAACAGT GCTGGAGAGG TCCGCACCCA GGCAGTACTG
    ATGGTGCAAG AGCCCCAGGA TGGCACCCAG CCCTGGTTCA TCAGCAAGCC CCGCTCACTG
    ACTGCCTCCC TGGGCCAGAC TGTCCTCATC TCCTGTGCCA TCGCTGGGGA CCCCTTCCCC
    ACTGTGCACT GGCTGCGAGA TGGCAAAGCC CTCTCCAAAG ACTCCGGCCA CTTCGAGGTG
    CTACAGAACG AGGACGTGTT CACCCTGGTT CTAAAGAATG TGCAGCCCTG GCACGCCGGC
    CAGTACGAGA TCCAGCTCAA GAACCGGGTT GGCGAATGCA GTTGCCAGGT GTCACTGATG
    CTACAGAGCA GCCCCACCGG AGCCCCCCTG CGGGGGAGGG AGCCTGCCAG CTGCGAGGGC
    CTCTGCAGCC GGGGAGCCGG CGCTGATGGT GGTGGCGGTG ACTGCTATGG GACCCTGAGG
    CCTGGCCGGC CAGCGAGAGG ACAGGGTTGG CCAGAGGAGG AGGACGACGG CGAGGACGTG
    CGGGGGGTGC TGAAGAGGCG CGTGGAGACC CGGCAACATA CTGAGGAGGC CATCCGCCAG
    CAGCAGGTGG AACAGCTGGA CTTCCGTGAC CTCCTGGGGA AGAAGGTGAG TACCAAGACC
    GTGTCAGAAG AAGACCTGAA GGAGATCCCA GCGGAGCAGA TGGATTTCCG CGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCA GAGGAAGAGA GGAAGGTGCA TAGCCCTCAG
    CAGGTCGACT TCCGCTCGGT CCTGGCCAAA AAGGGGACTC CTAAGACCCC GGTGCCTGAG
    AAGGCACCTC TTCCCAAACC CGCCACCCCA GATTTCCGCT CCGTGTTAGG TAGCAAGAAA
    AAATTACCAG CCGAGAATGG TAGCAACAAC GCTGAGGCCT TGAATGCCAA GGCAGCAGAA
    AGTCCCAAGG CTGTGAGCAA TGCCCAGCCT TTAGGGTCCC TGAAACCTTT GGGCAATGCC
    AAGCCTGCTG AGACTCTAAA ACCTGTGGGC AACATCAGGC CTGCTGAGAC GCTGAAACCC
    ATAGGCAACG CCAAACCTGC TGAGACCCTG AAACCCATGG GCAACGCCAA GCCTGCTGAG
    ACGCTAAAAC CCATAGGCAA TGCCAAGCTT GCTGAGACCC CGAAACCCAT GGGCAATGCC
    AAGCCTGCTG AGACCCTGAA ACCCCTGGGC AACGCCAAGC CTGCTGAGAC GCTGAAACCC
    ATAAGCAACG CCAAGCCTGC TGAACCTCTG AAACCTGTGG GCAACGCCAA GCCTGCTGAG
    ACCCCAAAAC CCATAGGTAA TGCCAAGCCT GCTGAGATCC TAAAACCAGC TGGGAAGGAA
    GAACTCAAGA AGGAGGTTAA GAATGACGTG AACTGCAAGA GGGCACAGGC AGGAGCCACA
    GATAATGAAA AAAGATCAGA GAACCAAGGG ACAGCCCCAG CCTTCAAGGA GAAGCTGCAA
    GATGTCCATG TGACAGAGGG TGAGAAGCTG CTACTCCAGT GTCAGGTGTC GTCTGACCCT
    CCAGCCACCA TCACCTGGAT GCTCAATGGA AAGACACTCA AGACCACCAA GTTCATCATC
    CTCTCCCAAG AAGGCTCACT GTGCTCCGTG TCCATCGAGA AGGCCCTGCC CGAGGACAGA
    GGCTTCTACA AGTGCATAGC TAAGAATGGC GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC
    ACCGTGGACG ATGCTCCCAC CAAGGAGAAT GCCAAGGCCC CGGAGATGAA AGCCCGGAGG
    CCCAAGAGCT CTCTACCTCC CATGCTAGGA ACAGAGAGTG ATGCAACTGT GAAAAAGAAA
    CCTGCCCCCA AGACACCTCC AAAAGCAGCC ATGCCCCCTC AGATCATCCA GTTTCCTGAG
    GACCAAAAGG TGCGCGCAGG AGAGTCTGTG GAGCTGTTTG GTAAAGTGGC AGGCACCCAG
    CCCATCACCT GTACGTGGAT GAAGTTCCGA AAGCAGATCC AGGAGAGCGA GCACATCAAG
    GTGGAGACCA GTGAGAATGG CAGCAGGCTC ACCATCGTGG CCGCACGCCA GGAACACTGC
    GGCTGCTACA CTCTGCTGGT GGAGAACAAG CTGGGCAGCA GGCAGGCCCA GGTCAACCTC
    ACTGTCGTGG ACAAACCAGA CCCCCCAGCG GGCACACCTT GCGCCTCCGA CATCCGGAGC
    TCGTCGTTGA CCCTGTCCTG GTATGGCTCC TCGTATGATG GGGGCAGTGC CGTGCAATCC
    TACAGCGTCG AGATCTGGGA CTCGGTGGAC AAGACGTGGA AGGAACTAGC CACGTGCCGC
    AGCACCTCTT TTAACGTCCA GGACCTGCTG CCTGACCGAG AGTATAAATT CCGCGTTCGC
    GCCATCAACG TGTATGGAAC CAGTGAGCCA AGCCAGGAGT CTGAACTCAC AGCAGTGGGA
    GAGAAACCGG AGGAGCCGAA GGACGAAGTG GAGGTGTCGG ATGATGATGA GAAGGAACCT
    GAGGTCGACT ACCGGACAGT GACGGTCAAC ACTGAACAAA AAGTGTCCGA CTTCTATGAC
    ATCGAAGAGA GACTAGGCTC TGGGAAATTT GGACAGGTCT TCCGACTTGT AGAAAAGAAA
    ACTGGAAAAA TCTGGGCAGG GAAATTCTTC AAGGCATATT CAGCCAAAGA GAAGGAGAAT
    ATCCGGCAGG AGATTAGCAT CATGAACTGC CTCCACCACC CCAAGCTGGT CCAGTGTGTG
    GATGCCTTCG AAGAAAAGGC CAACATCGTC ATGGTCCTGG AGATCGTGTC GGGGGGCGAG
    CTGTTCGAGC GCATCATCGA CGAGGACTTC GAGCTGACGG AGCGCGAGTG CATCCAGTAC
    ATGAGGCAGA TCTCGGCGGG CGTGGAGTAC ATCCACCGGC AGGGCATCGT CCACCTGGAC
    CTCAAGCCCG AGAACATCAT GTGCGTCAAT AAGACCGGCA CGAGGATCAA GCTCATCGAC
    TTCGGCCTGG CCAGAAAGCT GGAGAATGCG GGGTCTCTGA AGGTCCTCTT TGGTACCCCG
    GAATTTGTGG CCCCCGAAGT GATCAACTAT GAGCCCATCG GCTATGCCAC GGACATGTGG
    AGCATCGGAG TCATCTGCTA CATCCTGGTT AGTGGACTCT CCCCCTTCAT GGGTGACAAC
    GATAATGAAA CCCTAGCCAA CGTTACCTCA GCCACCTGGG ACTTCGATGA TGAGGCGTTC
    GATGAGATCT CGGATGACGC CAAGGATTTT ATCAGCAATT TGCTGAAGAA AGATATGAAA
    AACCGCCTGG ACTGTACCCA GTGCCTTCAG CATCCCTGGC TCATGAAAGA CACCAAGAAC
    ATGGAAGCCA AGAAACTCTC CAAGGACCGG ATGAAGAAGT ACATGGCAAG AAGGAAATGG
    CAGAAAACGG GCAATGCTGT GAGAGCCATC GGAAGACTGT CCTCTATGGC AATGATCTCA
    GGGCTCAGTG GCAGGAAATC CTCAACAGGA TCACCGACCA GCCCGCTCAA CGCAGAAAAA
    CTAGAATCTG AAGAAGATGT GTCCCAAGCT TTCCTTGAGG CTGTTGCTGA GGAAAAGCCC
    CATGTAAAAC CCTATTTCTC TAAGACCATT CGTGATTTAG AAGTTGTGGA GGGAAGCGCT
    GCTAGATTTG ACTGCAAGAT TGAAGGATAC CCAGACCCTG AAGTTGTCTG GTTTAAAGAT
    GACCAGTCAA TCCGGGAGTC CCGCCACTTC CAGATAGACT ACGACGAGGA TGGGAACTGC
    TCTTTAATCA TTAGCGATGT TTGTGGGGAT GACGATGCCA AGTACACCTG CAAGGCTGTC
    AATAGTCTTG GAGAAGCCAC TTGCACAGCA GAGCTCATTG TGGAGACCAT GGAGGAAGGG
    GAAGGGGAAG GGGAAGAGGA AGAAGAGGAA GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269594.1
    CDS16..5871
    Translation

    Target ORF information:

    RefSeq Version XM_022413886.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGATACATC TGACGATCCA GAAGGTCCGG GGTTACCCAG AGCCCCAGGT GACATGGCAC 
    CGAAATGGGC AACCCATCAC CAGTGGGGGC CGCTTCCTGC TGGATTGTGG TATCCGGGGG
    ACTTTCAGCC TCGTGATTCA TGCTGTCCGT GAGGAGGACC AGGGAAAGTA TACCTGCGAA
    GCCACCAATG ATGGTGGTGC CCGCCAGGTG ACAGTGGAGT TGACTGTAGA AGGGGGCTTC
    ATGAAGAAGC ATGGTCAGCC CGTTGTTTCC AAAACCTTGG GGGACAGATT TGCAGCCCCC
    GCAGTGGAGA CCCGTCCTAG CATCTGGGGA GAGTGCCCAC CAAAGTTTGC TACCAAGTTG
    GGCCGAGCCG TAGTCAAAGA AGGACAGACG GGACGGTTCT CCTGCAAGAT CACTGGACGG
    CCCCAACCAC AGGTCACCTG GCTCAAGGGG GATGTTGCCC TGCAGCCAAG TGCCCGTGTG
    TCTATGTCTG AGAAGAACGG GGTACAAGTT CTGGAAATCC ATGAGGTCAG CCGCGATGAT
    GTGGGAGTGT ACACATGCAT GGTGGTGAAT GGGTCGGGGA AGGCCTCCAT GTCGGCTGAG
    CTTTCTATCC AAGGTTTGGA CAATACCAGT AGGTCATTAA CAAAGGTGGC CAATTCAGAT
    ATCAGGAAGG AGGTGACCAA TGGAACCACG CAGGGGCCGA AACTAGACCA CCTGGAGTCT
    GCAGCGGAAA GGTGGCCCTG CTCCAGCACC CAGATCGGTG GCTCCCCCAC CTGGGCCACA
    AGTAGCCAGC CTCAGCCCCT GAGGGAGTCC AAGCTGGAGC CGCCTGGGGA CCCACCCAGG
    AAAGCCCTGA GGAGCCCCGT CCTACAGAAG ACTTCCAGCA CCATTACCCT GCAGGCGGCA
    AAAGTCCAGC CAGAGCCAAG AGCGCCGGTC TCAAGTGCCC TCTCGTCTCC CGGAGAAGAG
    AGGGAGAGGC CAGCTGCTCC CCCCACAGCC ACCTTCCCTG CCCGGCAGTC TGGCCTGGGA
    AGCCAGGAGG TTGTGAACAA GGTTGCTACC AGAAAAATCC CCATGGAAAG CCAGAGAGAT
    TCCACATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAGTGA AGATGAAACA
    GTCAAGTTCA GATGTGAGGT TTCAGGGACC CCAAAGCCTG AAGTGACCTG GTTCCTGGAA
    GGTGCCCCCG TGAAGAGACG AGAAGGCATG GTTGAGGTTT ATGAAGAAGG TGGATGCCAC
    TACCTCTGCT TGCTGAGAGT GCGGGCCAAG GACAGTGGGA GCTACAGTTG CACGGCCTCC
    AATGTCCGAG GCCAGGTGTC CTGTGGTTGG ACCCTCCTGG TGAAACGGCT GGCCGTGATG
    GAGGTGGCTC CCTCGTTCTC CAGCGTCCTG AAGGACTGCG CTGTCATTGA GGGCCAGGAC
    TTTGTGCTGC AGTGCTCAGT GCAGGGGACC CCGGTGCCCC AAATCACTTG GCTGCTTAAT
    GAGCAGCCCA TCCAGTACGC TCACTCCTCC TGCGAGGCTG GCGTGGCCGA GCTCCACATC
    CAGGATGCCC TGCCGGAAGA TGATGGCATT TACACCTGTC TGGCCAAGAA CACCTTGGGC
    CAGGTGTCCT GCAGCGCCCG GGTCACCGTC CATGAAAAGA AGAGCGACAG GAAGAGTGGG
    TATCTTCTGC GCACAGCTCC CGGCAAGCCC GTCGCACCCC TCTTCCTGCA GGGCCTCTCT
    GACCTCAAAG TCATGGATGG AAGCCAGGTC ACCATGACAG TCCAGGTGTC AGGGAATCCG
    CCCCCCGAGG TCATCTGGCT TCACAACGGG AATGAGATCC AGGAGTCAGA GGACTTCCAC
    TTTGAACAGA GAGGCACTCA GCACAGCCTT TGTATCCAGG AAGTGTTCCC AGAGGACACA
    GGCACTTACA CCTGCGAAGC CTGGAACAGT GCTGGAGAGG TCCGCACCCA GGCAGTACTG
    ATGGTGCAAG AGCCCCAGGA TGGCACCCAG CCCTGGTTCA TCAGCAAGCC CCGCTCACTG
    ACTGCCTCCC TGGGCCAGAC TGTCCTCATC TCCTGTGCCA TCGCTGGGGA CCCCTTCCCC
    ACTGTGCACT GGCTGCGAGA TGGCAAAGCC CTCTCCAAAG ACTCCGGCCA CTTCGAGGTG
    CTACAGAACG AGGACGTGTT CACCCTGGTT CTAAAGAATG TGCAGCCCTG GCACGCCGGC
    CAGTACGAGA TCCAGCTCAA GAACCGGGTT GGCGAATGCA GTTGCCAGGT GTCACTGATG
    CTACAGAGCA GCCCCACCGG AGCCCCCCTG CGGGGGAGGG AGCCTGCCAG CTGCGAGGGC
    CTCTGCAGCC GGGGAGCCGG CGCTGATGGT GGTGGCGGTG ACTGCTATGG GACCCTGAGG
    CCTGGCCGGC CAGCGAGAGG ACAGGGTTGG CCAGAGGAGG AGGACGACGG CGAGGACGTG
    CGGGGGGTGC TGAAGAGGCG CGTGGAGACC CGGCAACATA CTGAGGAGGC CATCCGCCAG
    CAGCAGGTGG AACAGCTGGA CTTCCGTGAC CTCCTGGGGA AGAAGGTGAG TACCAAGACC
    GTGTCAGAAG AAGACCTGAA GGAGATCCCA GCGGAGCAGA TGGATTTCCG CGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCA GAGGAAGAGA GGAAGGTGCA TAGCCCTCAG
    CAGGTCGACT TCCGCTCGGT CCTGGCCAAA AAGGGGACTC CTAAGACCCC GGTGCCTGAG
    AAGGCACCTC TTCCCAAACC CGCCACCCCA GATTTCCGCT CCGTGTTAGG TAGCAAGAAA
    AAATTACCAG CCGAGAATGG TAGCAACAAC GCTGAGGCCT TGAATGCCAA GGCAGCAGAA
    AGTCCCAAGG CTGTGAGCAA TGCCCAGCCT TTAGGGTCCC TGAAACCTTT GGGCAATGCC
    AAGCCTGCTG AGACTCTAAA ACCTGTGGGC AACATCAGGC CTGCTGAGAC GCTGAAACCC
    ATAGGCAACG CCAAACCTGC TGAGACCCTG AAACCCATGG GCAACGCCAA GCCTGCTGAG
    ACGCTAAAAC CCATAGGCAA TGCCAAGCTT GCTGAGACCC CGAAACCCAT GGGCAATGCC
    AAGCCTGCTG AGACCCTGAA ACCCCTGGGC AACGCCAAGC CTGCTGAGAC GCTGAAACCC
    ATAAGCAACG CCAAGCCTGC TGAACCTCTG AAACCTGTGG GCAACGCCAA GCCTGCTGAG
    ACCCCAAAAC CCATAGGTAA TGCCAAGCCT GCTGAGATCC TAAAACCAGC TGGGAAGGAA
    GAACTCAAGA AGGAGGTTAA GAATGACGTG AACTGCAAGA GGGCACAGGC AGGAGCCACA
    GATAATGAAA AAAGATCAGA GAACCAAGGG ACAGCCCCAG CCTTCAAGGA GAAGCTGCAA
    GATGTCCATG TGACAGAGGG TGAGAAGCTG CTACTCCAGT GTCAGGTGTC GTCTGACCCT
    CCAGCCACCA TCACCTGGAT GCTCAATGGA AAGACACTCA AGACCACCAA GTTCATCATC
    CTCTCCCAAG AAGGCTCACT GTGCTCCGTG TCCATCGAGA AGGCCCTGCC CGAGGACAGA
    GGCTTCTACA AGTGCATAGC TAAGAATGGC GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC
    ACCGTGGACG ATGCTCCCAC CAAGGAGAAT GCCAAGGCCC CGGAGATGAA AGCCCGGAGG
    CCCAAGAGCT CTCTACCTCC CATGCTAGGA ACAGAGAGTG ATGCAACTGT GAAAAAGAAA
    CCTGCCCCCA AGACACCTCC AAAAGCAGCC ATGCCCCCTC AGATCATCCA GTTTCCTGAG
    GACCAAAAGG TGCGCGCAGG AGAGTCTGTG GAGCTGTTTG GTAAAGTGGC AGGCACCCAG
    CCCATCACCT GTACGTGGAT GAAGTTCCGA AAGCAGATCC AGGAGAGCGA GCACATCAAG
    GTGGAGACCA GTGAGAATGG CAGCAGGCTC ACCATCGTGG CCGCACGCCA GGAACACTGC
    GGCTGCTACA CTCTGCTGGT GGAGAACAAG CTGGGCAGCA GGCAGGCCCA GGTCAACCTC
    ACTGTCGTGG ACAAACCAGA CCCCCCAGCG GGCACACCTT GCGCCTCCGA CATCCGGAGC
    TCGTCGTTGA CCCTGTCCTG GTATGGCTCC TCGTATGATG GGGGCAGTGC CGTGCAATCC
    TACAGCGTCG AGATCTGGGA CTCGGTGGAC AAGACGTGGA AGGAACTAGC CACGTGCCGC
    AGCACCTCTT TTAACGTCCA GGACCTGCTG CCTGACCGAG AGTATAAATT CCGCGTTCGC
    GCCATCAACG TGTATGGAAC CAGTGAGCCA AGCCAGGAGT CTGAACTCAC AGCAGTGGGA
    GAGAAACCGG AGGAGCCGAA GGACGAAGTG GAGGTGTCGG ATGATGATGA GAAGGAACCT
    GAGGTCGACT ACCGGACAGT GACGGTCAAC ACTGAACAAA AAGTGTCCGA CTTCTATGAC
    ATCGAAGAGA GACTAGGCTC TGGGAAATTT GGACAGGTCT TCCGACTTGT AGAAAAGAAA
    ACTGGAAAAA TCTGGGCAGG GAAATTCTTC AAGGCATATT CAGCCAAAGA GAAGGAGAAT
    ATCCGGCAGG AGATTAGCAT CATGAACTGC CTCCACCACC CCAAGCTGGT CCAGTGTGTG
    GATGCCTTCG AAGAAAAGGC CAACATCGTC ATGGTCCTGG AGATCGTGTC GGGGGGCGAG
    CTGTTCGAGC GCATCATCGA CGAGGACTTC GAGCTGACGG AGCGCGAGTG CATCCAGTAC
    ATGAGGCAGA TCTCGGCGGG CGTGGAGTAC ATCCACCGGC AGGGCATCGT CCACCTGGAC
    CTCAAGCCCG AGAACATCAT GTGCGTCAAT AAGACCGGCA CGAGGATCAA GCTCATCGAC
    TTCGGCCTGG CCAGAAAGCT GGAGAATGCG GGGTCTCTGA AGGTCCTCTT TGGTACCCCG
    GAATTTGTGG CCCCCGAAGT GATCAACTAT GAGCCCATCG GCTATGCCAC GGACATGTGG
    AGCATCGGAG TCATCTGCTA CATCCTGGTT AGTGGACTCT CCCCCTTCAT GGGTGACAAC
    GATAATGAAA CCCTAGCCAA CGTTACCTCA GCCACCTGGG ACTTCGATGA TGAGGCGTTC
    GATGAGATCT CGGATGACGC CAAGGATTTT ATCAGCAATT TGCTGAAGAA AGATATGAAA
    AACCGCCTGG ACTGTACCCA GTGCCTTCAG CATCCCTGGC TCATGAAAGA CACCAAGAAC
    ATGGAAGCCA AGAAACTCTC CAAGGACCGG ATGAAGAAGT ACATGGCAAG AAGGAAATGG
    CAGAAAACGG GCAATGCTGT GAGAGCCATC GGAAGACTGT CCTCTATGGC AATGATCTCA
    GGGCTCAGTG GCAGGAAATC CTCAACAGGA TCACCGACCA GCCCGCTCAA CGCAGAAAAA
    CTAGAATCTG AAGAAGATGT GTCCCAAGCT TTCCTTGAGG CTGTTGCTGA GGAAAAGCCC
    CATGTAAAAC CCTATTTCTC TAAGACCATT CGTGATTTAG AAGTTGTGGA GGGAAGCGCT
    GCTAGATTTG ACTGCAAGAT TGAAGGATAC CCAGACCCTG AAGTTGTCTG GTTTAAAGAT
    GACCAGTCAA TCCGGGAGTC CCGCCACTTC CAGATAGACT ACGACGAGGA TGGGAACTGC
    TCTTTAATCA TTAGCGATGT TTGTGGGGAT GACGATGCCA AGTACACCTG CAAGGCTGTC
    AATAGTCTTG GAGAAGCCAC TTGCACAGCA GAGCTCATTG TGGAGACCAT GGAGGAAGGG
    GAAGGGGAAG GGGAAGAGGA AGAAGAGGAA GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284277
    Clone ID Related Accession (Same CDS sequence) XM_022413887.1
    Accession Version XM_022413887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5616bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myosin light chain kinase, smooth muscle isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGAAGC ATGGTCAGCC CGTTGTTTCC AAAACCTTGG GGGACAGATT TGCAGCCCCC 
    GCAGTGGAGA CCCGTCCTAG CATCTGGGGA GAGTGCCCAC CAAAGTTTGC TACCAAGTTG
    GGCCGAGCCG TAGTCAAAGA AGGACAGACG GGACGGTTCT CCTGCAAGAT CACTGGACGG
    CCCCAACCAC AGGTCACCTG GCTCAAGGGG GATGTTGCCC TGCAGCCAAG TGCCCGTGTG
    TCTATGTCTG AGAAGAACGG GGTACAAGTT CTGGAAATCC ATGAGGTCAG CCGCGATGAT
    GTGGGAGTGT ACACATGCAT GGTGGTGAAT GGGTCGGGGA AGGCCTCCAT GTCGGCTGAG
    CTTTCTATCC AAGGTTTGGA CAATACCAGT AGGTCATTAA CAAAGGTGGC CAATTCAGAT
    ATCAGGAAGG AGGTGACCAA TGGAACCACG CAGGGGCCGA AACTAGACCA CCTGGAGTCT
    GCAGCGGAAA GGTGGCCCTG CTCCAGCACC CAGATCGGTG GCTCCCCCAC CTGGGCCACA
    AGTAGCCAGC CTCAGCCCCT GAGGGAGTCC AAGCTGGAGC CGCCTGGGGA CCCACCCAGG
    AAAGCCCTGA GGAGCCCCGT CCTACAGAAG ACTTCCAGCA CCATTACCCT GCAGGCGGCA
    AAAGTCCAGC CAGAGCCAAG AGCGCCGGTC TCAAGTGCCC TCTCGTCTCC CGGAGAAGAG
    AGGGAGAGGC CAGCTGCTCC CCCCACAGCC ACCTTCCCTG CCCGGCAGTC TGGCCTGGGA
    AGCCAGGAGG TTGTGAACAA GGTTGCTACC AGAAAAATCC CCATGGAAAG CCAGAGAGAT
    TCCACATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAGTGA AGATGAAACA
    GTCAAGTTCA GATGTGAGGT TTCAGGGACC CCAAAGCCTG AAGTGACCTG GTTCCTGGAA
    GGTGCCCCCG TGAAGAGACG AGAAGGCATG GTTGAGGTTT ATGAAGAAGG TGGATGCCAC
    TACCTCTGCT TGCTGAGAGT GCGGGCCAAG GACAGTGGGA GCTACAGTTG CACGGCCTCC
    AATGTCCGAG GCCAGGTGTC CTGTGGTTGG ACCCTCCTGG TGAAACGGCT GGCCGTGATG
    GAGGTGGCTC CCTCGTTCTC CAGCGTCCTG AAGGACTGCG CTGTCATTGA GGGCCAGGAC
    TTTGTGCTGC AGTGCTCAGT GCAGGGGACC CCGGTGCCCC AAATCACTTG GCTGCTTAAT
    GAGCAGCCCA TCCAGTACGC TCACTCCTCC TGCGAGGCTG GCGTGGCCGA GCTCCACATC
    CAGGATGCCC TGCCGGAAGA TGATGGCATT TACACCTGTC TGGCCAAGAA CACCTTGGGC
    CAGGTGTCCT GCAGCGCCCG GGTCACCGTC CATGAAAAGA AGAGCGACAG GAAGAGTGGG
    TATCTTCTGC GCACAGCTCC CGGCAAGCCC GTCGCACCCC TCTTCCTGCA GGGCCTCTCT
    GACCTCAAAG TCATGGATGG AAGCCAGGTC ACCATGACAG TCCAGGTGTC AGGGAATCCG
    CCCCCCGAGG TCATCTGGCT TCACAACGGG AATGAGATCC AGGAGTCAGA GGACTTCCAC
    TTTGAACAGA GAGGCACTCA GCACAGCCTT TGTATCCAGG AAGTGTTCCC AGAGGACACA
    GGCACTTACA CCTGCGAAGC CTGGAACAGT GCTGGAGAGG TCCGCACCCA GGCAGTACTG
    ATGGTGCAAG AGCCCCAGGA TGGCACCCAG CCCTGGTTCA TCAGCAAGCC CCGCTCACTG
    ACTGCCTCCC TGGGCCAGAC TGTCCTCATC TCCTGTGCCA TCGCTGGGGA CCCCTTCCCC
    ACTGTGCACT GGCTGCGAGA TGGCAAAGCC CTCTCCAAAG ACTCCGGCCA CTTCGAGGTG
    CTACAGAACG AGGACGTGTT CACCCTGGTT CTAAAGAATG TGCAGCCCTG GCACGCCGGC
    CAGTACGAGA TCCAGCTCAA GAACCGGGTT GGCGAATGCA GTTGCCAGGT GTCACTGATG
    CTACAGAGCA GCCCCACCGG AGCCCCCCTG CGGGGGAGGG AGCCTGCCAG CTGCGAGGGC
    CTCTGCAGCC GGGGAGCCGG CGCTGATGGT GGTGGCGGTG ACTGCTATGG GACCCTGAGG
    CCTGGCCGGC CAGCGAGAGG ACAGGGTTGG CCAGAGGAGG AGGACGACGG CGAGGACGTG
    CGGGGGGTGC TGAAGAGGCG CGTGGAGACC CGGCAACATA CTGAGGAGGC CATCCGCCAG
    CAGCAGGTGG AACAGCTGGA CTTCCGTGAC CTCCTGGGGA AGAAGGTGAG TACCAAGACC
    GTGTCAGAAG AAGACCTGAA GGAGATCCCA GCGGAGCAGA TGGATTTCCG CGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCA GAGGAAGAGA GGAAGGTGCA TAGCCCTCAG
    CAGGTCGACT TCCGCTCGGT CCTGGCCAAA AAGGGGACTC CTAAGACCCC GGTGCCTGAG
    AAGGCACCTC TTCCCAAACC CGCCACCCCA GATTTCCGCT CCGTGTTAGG TAGCAAGAAA
    AAATTACCAG CCGAGAATGG TAGCAACAAC GCTGAGGCCT TGAATGCCAA GGCAGCAGAA
    AGTCCCAAGG CTGTGAGCAA TGCCCAGCCT TTAGGGTCCC TGAAACCTTT GGGCAATGCC
    AAGCCTGCTG AGACTCTAAA ACCTGTGGGC AACATCAGGC CTGCTGAGAC GCTGAAACCC
    ATAGGCAACG CCAAACCTGC TGAGACCCTG AAACCCATGG GCAACGCCAA GCCTGCTGAG
    ACGCTAAAAC CCATAGGCAA TGCCAAGCTT GCTGAGACCC CGAAACCCAT GGGCAATGCC
    AAGCCTGCTG AGACCCTGAA ACCCCTGGGC AACGCCAAGC CTGCTGAGAC GCTGAAACCC
    ATAAGCAACG CCAAGCCTGC TGAACCTCTG AAACCTGTGG GCAACGCCAA GCCTGCTGAG
    ACCCCAAAAC CCATAGGTAA TGCCAAGCCT GCTGAGATCC TAAAACCAGC TGGGAAGGAA
    GAACTCAAGA AGGAGGTTAA GAATGACGTG AACTGCAAGA GGGCACAGGC AGGAGCCACA
    GATAATGAAA AAAGATCAGA GAACCAAGGG ACAGCCCCAG CCTTCAAGGA GAAGCTGCAA
    GATGTCCATG TGACAGAGGG TGAGAAGCTG CTACTCCAGT GTCAGGTGTC GTCTGACCCT
    CCAGCCACCA TCACCTGGAT GCTCAATGGA AAGACACTCA AGACCACCAA GTTCATCATC
    CTCTCCCAAG AAGGCTCACT GTGCTCCGTG TCCATCGAGA AGGCCCTGCC CGAGGACAGA
    GGCTTCTACA AGTGCATAGC TAAGAATGGC GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC
    ACCGTGGACG ATGCTCCCAC CAAGGAGAAT GCCAAGGCCC CGGAGATGAA AGCCCGGAGG
    CCCAAGAGCT CTCTACCTCC CATGCTAGGA ACAGAGAGTG ATGCAACTGT GAAAAAGAAA
    CCTGCCCCCA AGACACCTCC AAAAGCAGCC ATGCCCCCTC AGATCATCCA GTTTCCTGAG
    GACCAAAAGG TGCGCGCAGG AGAGTCTGTG GAGCTGTTTG GTAAAGTGGC AGGCACCCAG
    CCCATCACCT GTACGTGGAT GAAGTTCCGA AAGCAGATCC AGGAGAGCGA GCACATCAAG
    GTGGAGACCA GTGAGAATGG CAGCAGGCTC ACCATCGTGG CCGCACGCCA GGAACACTGC
    GGCTGCTACA CTCTGCTGGT GGAGAACAAG CTGGGCAGCA GGCAGGCCCA GGTCAACCTC
    ACTGTCGTGG ACAAACCAGA CCCCCCAGCG GGCACACCTT GCGCCTCCGA CATCCGGAGC
    TCGTCGTTGA CCCTGTCCTG GTATGGCTCC TCGTATGATG GGGGCAGTGC CGTGCAATCC
    TACAGCGTCG AGATCTGGGA CTCGGTGGAC AAGACGTGGA AGGAACTAGC CACGTGCCGC
    AGCACCTCTT TTAACGTCCA GGACCTGCTG CCTGACCGAG AGTATAAATT CCGCGTTCGC
    GCCATCAACG TGTATGGAAC CAGTGAGCCA AGCCAGGAGT CTGAACTCAC AGCAGTGGGA
    GAGAAACCGG AGGAGCCGAA GGACGAAGTG GAGGTGTCGG ATGATGATGA GAAGGAACCT
    GAGGTCGACT ACCGGACAGT GACGGTCAAC ACTGAACAAA AAGTGTCCGA CTTCTATGAC
    ATCGAAGAGA GACTAGGCTC TGGGAAATTT GGACAGGTCT TCCGACTTGT AGAAAAGAAA
    ACTGGAAAAA TCTGGGCAGG GAAATTCTTC AAGGCATATT CAGCCAAAGA GAAGGAGAAT
    ATCCGGCAGG AGATTAGCAT CATGAACTGC CTCCACCACC CCAAGCTGGT CCAGTGTGTG
    GATGCCTTCG AAGAAAAGGC CAACATCGTC ATGGTCCTGG AGATCGTGTC GGGGGGCGAG
    CTGTTCGAGC GCATCATCGA CGAGGACTTC GAGCTGACGG AGCGCGAGTG CATCCAGTAC
    ATGAGGCAGA TCTCGGCGGG CGTGGAGTAC ATCCACCGGC AGGGCATCGT CCACCTGGAC
    CTCAAGCCCG AGAACATCAT GTGCGTCAAT AAGACCGGCA CGAGGATCAA GCTCATCGAC
    TTCGGCCTGG CCAGAAAGCT GGAGAATGCG GGGTCTCTGA AGGTCCTCTT TGGTACCCCG
    GAATTTGTGG CCCCCGAAGT GATCAACTAT GAGCCCATCG GCTATGCCAC GGACATGTGG
    AGCATCGGAG TCATCTGCTA CATCCTGGTT AGTGGACTCT CCCCCTTCAT GGGTGACAAC
    GATAATGAAA CCCTAGCCAA CGTTACCTCA GCCACCTGGG ACTTCGATGA TGAGGCGTTC
    GATGAGATCT CGGATGACGC CAAGGATTTT ATCAGCAATT TGCTGAAGAA AGATATGAAA
    AACCGCCTGG ACTGTACCCA GTGCCTTCAG CATCCCTGGC TCATGAAAGA CACCAAGAAC
    ATGGAAGCCA AGAAACTCTC CAAGGACCGG ATGAAGAAGT ACATGGCAAG AAGGAAATGG
    CAGAAAACGG GCAATGCTGT GAGAGCCATC GGAAGACTGT CCTCTATGGC AATGATCTCA
    GGGCTCAGTG GCAGGAAATC CTCAACAGGA TCACCGACCA GCCCGCTCAA CGCAGAAAAA
    CTAGAATCTG AAGAAGATGT GTCCCAAGCT TTCCTTGAGG CTGTTGCTGA GGAAAAGCCC
    CATGTAAAAC CCTATTTCTC TAAGACCATT CGTGATTTAG AAGTTGTGGA GGGAAGCGCT
    GCTAGATTTG ACTGCAAGAT TGAAGGATAC CCAGACCCTG AAGTTGTCTG GTTTAAAGAT
    GACCAGTCAA TCCGGGAGTC CCGCCACTTC CAGATAGACT ACGACGAGGA TGGGAACTGC
    TCTTTAATCA TTAGCGATGT TTGTGGGGAT GACGATGCCA AGTACACCTG CAAGGCTGTC
    AATAGTCTTG GAGAAGCCAC TTGCACAGCA GAGCTCATTG TGGAGACCAT GGAGGAAGGG
    GAAGGGGAAG GGGAAGAGGA AGAAGAGGAA GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269595.1
    CDS454..6069
    Translation

    Target ORF information:

    RefSeq Version XM_022413887.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGAAGAAGC ATGGTCAGCC CGTTGTTTCC AAAACCTTGG GGGACAGATT TGCAGCCCCC 
    GCAGTGGAGA CCCGTCCTAG CATCTGGGGA GAGTGCCCAC CAAAGTTTGC TACCAAGTTG
    GGCCGAGCCG TAGTCAAAGA AGGACAGACG GGACGGTTCT CCTGCAAGAT CACTGGACGG
    CCCCAACCAC AGGTCACCTG GCTCAAGGGG GATGTTGCCC TGCAGCCAAG TGCCCGTGTG
    TCTATGTCTG AGAAGAACGG GGTACAAGTT CTGGAAATCC ATGAGGTCAG CCGCGATGAT
    GTGGGAGTGT ACACATGCAT GGTGGTGAAT GGGTCGGGGA AGGCCTCCAT GTCGGCTGAG
    CTTTCTATCC AAGGTTTGGA CAATACCAGT AGGTCATTAA CAAAGGTGGC CAATTCAGAT
    ATCAGGAAGG AGGTGACCAA TGGAACCACG CAGGGGCCGA AACTAGACCA CCTGGAGTCT
    GCAGCGGAAA GGTGGCCCTG CTCCAGCACC CAGATCGGTG GCTCCCCCAC CTGGGCCACA
    AGTAGCCAGC CTCAGCCCCT GAGGGAGTCC AAGCTGGAGC CGCCTGGGGA CCCACCCAGG
    AAAGCCCTGA GGAGCCCCGT CCTACAGAAG ACTTCCAGCA CCATTACCCT GCAGGCGGCA
    AAAGTCCAGC CAGAGCCAAG AGCGCCGGTC TCAAGTGCCC TCTCGTCTCC CGGAGAAGAG
    AGGGAGAGGC CAGCTGCTCC CCCCACAGCC ACCTTCCCTG CCCGGCAGTC TGGCCTGGGA
    AGCCAGGAGG TTGTGAACAA GGTTGCTACC AGAAAAATCC CCATGGAAAG CCAGAGAGAT
    TCCACATTCC CCAAATTTGA GAGCAAGCCC CAAAGCCAGG AGGTCAGTGA AGATGAAACA
    GTCAAGTTCA GATGTGAGGT TTCAGGGACC CCAAAGCCTG AAGTGACCTG GTTCCTGGAA
    GGTGCCCCCG TGAAGAGACG AGAAGGCATG GTTGAGGTTT ATGAAGAAGG TGGATGCCAC
    TACCTCTGCT TGCTGAGAGT GCGGGCCAAG GACAGTGGGA GCTACAGTTG CACGGCCTCC
    AATGTCCGAG GCCAGGTGTC CTGTGGTTGG ACCCTCCTGG TGAAACGGCT GGCCGTGATG
    GAGGTGGCTC CCTCGTTCTC CAGCGTCCTG AAGGACTGCG CTGTCATTGA GGGCCAGGAC
    TTTGTGCTGC AGTGCTCAGT GCAGGGGACC CCGGTGCCCC AAATCACTTG GCTGCTTAAT
    GAGCAGCCCA TCCAGTACGC TCACTCCTCC TGCGAGGCTG GCGTGGCCGA GCTCCACATC
    CAGGATGCCC TGCCGGAAGA TGATGGCATT TACACCTGTC TGGCCAAGAA CACCTTGGGC
    CAGGTGTCCT GCAGCGCCCG GGTCACCGTC CATGAAAAGA AGAGCGACAG GAAGAGTGGG
    TATCTTCTGC GCACAGCTCC CGGCAAGCCC GTCGCACCCC TCTTCCTGCA GGGCCTCTCT
    GACCTCAAAG TCATGGATGG AAGCCAGGTC ACCATGACAG TCCAGGTGTC AGGGAATCCG
    CCCCCCGAGG TCATCTGGCT TCACAACGGG AATGAGATCC AGGAGTCAGA GGACTTCCAC
    TTTGAACAGA GAGGCACTCA GCACAGCCTT TGTATCCAGG AAGTGTTCCC AGAGGACACA
    GGCACTTACA CCTGCGAAGC CTGGAACAGT GCTGGAGAGG TCCGCACCCA GGCAGTACTG
    ATGGTGCAAG AGCCCCAGGA TGGCACCCAG CCCTGGTTCA TCAGCAAGCC CCGCTCACTG
    ACTGCCTCCC TGGGCCAGAC TGTCCTCATC TCCTGTGCCA TCGCTGGGGA CCCCTTCCCC
    ACTGTGCACT GGCTGCGAGA TGGCAAAGCC CTCTCCAAAG ACTCCGGCCA CTTCGAGGTG
    CTACAGAACG AGGACGTGTT CACCCTGGTT CTAAAGAATG TGCAGCCCTG GCACGCCGGC
    CAGTACGAGA TCCAGCTCAA GAACCGGGTT GGCGAATGCA GTTGCCAGGT GTCACTGATG
    CTACAGAGCA GCCCCACCGG AGCCCCCCTG CGGGGGAGGG AGCCTGCCAG CTGCGAGGGC
    CTCTGCAGCC GGGGAGCCGG CGCTGATGGT GGTGGCGGTG ACTGCTATGG GACCCTGAGG
    CCTGGCCGGC CAGCGAGAGG ACAGGGTTGG CCAGAGGAGG AGGACGACGG CGAGGACGTG
    CGGGGGGTGC TGAAGAGGCG CGTGGAGACC CGGCAACATA CTGAGGAGGC CATCCGCCAG
    CAGCAGGTGG AACAGCTGGA CTTCCGTGAC CTCCTGGGGA AGAAGGTGAG TACCAAGACC
    GTGTCAGAAG AAGACCTGAA GGAGATCCCA GCGGAGCAGA TGGATTTCCG CGCCAACCTG
    CAGCGGCAAG TGAAGCCAAA GACTGTGTCA GAGGAAGAGA GGAAGGTGCA TAGCCCTCAG
    CAGGTCGACT TCCGCTCGGT CCTGGCCAAA AAGGGGACTC CTAAGACCCC GGTGCCTGAG
    AAGGCACCTC TTCCCAAACC CGCCACCCCA GATTTCCGCT CCGTGTTAGG TAGCAAGAAA
    AAATTACCAG CCGAGAATGG TAGCAACAAC GCTGAGGCCT TGAATGCCAA GGCAGCAGAA
    AGTCCCAAGG CTGTGAGCAA TGCCCAGCCT TTAGGGTCCC TGAAACCTTT GGGCAATGCC
    AAGCCTGCTG AGACTCTAAA ACCTGTGGGC AACATCAGGC CTGCTGAGAC GCTGAAACCC
    ATAGGCAACG CCAAACCTGC TGAGACCCTG AAACCCATGG GCAACGCCAA GCCTGCTGAG
    ACGCTAAAAC CCATAGGCAA TGCCAAGCTT GCTGAGACCC CGAAACCCAT GGGCAATGCC
    AAGCCTGCTG AGACCCTGAA ACCCCTGGGC AACGCCAAGC CTGCTGAGAC GCTGAAACCC
    ATAAGCAACG CCAAGCCTGC TGAACCTCTG AAACCTGTGG GCAACGCCAA GCCTGCTGAG
    ACCCCAAAAC CCATAGGTAA TGCCAAGCCT GCTGAGATCC TAAAACCAGC TGGGAAGGAA
    GAACTCAAGA AGGAGGTTAA GAATGACGTG AACTGCAAGA GGGCACAGGC AGGAGCCACA
    GATAATGAAA AAAGATCAGA GAACCAAGGG ACAGCCCCAG CCTTCAAGGA GAAGCTGCAA
    GATGTCCATG TGACAGAGGG TGAGAAGCTG CTACTCCAGT GTCAGGTGTC GTCTGACCCT
    CCAGCCACCA TCACCTGGAT GCTCAATGGA AAGACACTCA AGACCACCAA GTTCATCATC
    CTCTCCCAAG AAGGCTCACT GTGCTCCGTG TCCATCGAGA AGGCCCTGCC CGAGGACAGA
    GGCTTCTACA AGTGCATAGC TAAGAATGGC GCCGGCCAGG CTGAGTGCTC CTGCCAAGTC
    ACCGTGGACG ATGCTCCCAC CAAGGAGAAT GCCAAGGCCC CGGAGATGAA AGCCCGGAGG
    CCCAAGAGCT CTCTACCTCC CATGCTAGGA ACAGAGAGTG ATGCAACTGT GAAAAAGAAA
    CCTGCCCCCA AGACACCTCC AAAAGCAGCC ATGCCCCCTC AGATCATCCA GTTTCCTGAG
    GACCAAAAGG TGCGCGCAGG AGAGTCTGTG GAGCTGTTTG GTAAAGTGGC AGGCACCCAG
    CCCATCACCT GTACGTGGAT GAAGTTCCGA AAGCAGATCC AGGAGAGCGA GCACATCAAG
    GTGGAGACCA GTGAGAATGG CAGCAGGCTC ACCATCGTGG CCGCACGCCA GGAACACTGC
    GGCTGCTACA CTCTGCTGGT GGAGAACAAG CTGGGCAGCA GGCAGGCCCA GGTCAACCTC
    ACTGTCGTGG ACAAACCAGA CCCCCCAGCG GGCACACCTT GCGCCTCCGA CATCCGGAGC
    TCGTCGTTGA CCCTGTCCTG GTATGGCTCC TCGTATGATG GGGGCAGTGC CGTGCAATCC
    TACAGCGTCG AGATCTGGGA CTCGGTGGAC AAGACGTGGA AGGAACTAGC CACGTGCCGC
    AGCACCTCTT TTAACGTCCA GGACCTGCTG CCTGACCGAG AGTATAAATT CCGCGTTCGC
    GCCATCAACG TGTATGGAAC CAGTGAGCCA AGCCAGGAGT CTGAACTCAC AGCAGTGGGA
    GAGAAACCGG AGGAGCCGAA GGACGAAGTG GAGGTGTCGG ATGATGATGA GAAGGAACCT
    GAGGTCGACT ACCGGACAGT GACGGTCAAC ACTGAACAAA AAGTGTCCGA CTTCTATGAC
    ATCGAAGAGA GACTAGGCTC TGGGAAATTT GGACAGGTCT TCCGACTTGT AGAAAAGAAA
    ACTGGAAAAA TCTGGGCAGG GAAATTCTTC AAGGCATATT CAGCCAAAGA GAAGGAGAAT
    ATCCGGCAGG AGATTAGCAT CATGAACTGC CTCCACCACC CCAAGCTGGT CCAGTGTGTG
    GATGCCTTCG AAGAAAAGGC CAACATCGTC ATGGTCCTGG AGATCGTGTC GGGGGGCGAG
    CTGTTCGAGC GCATCATCGA CGAGGACTTC GAGCTGACGG AGCGCGAGTG CATCCAGTAC
    ATGAGGCAGA TCTCGGCGGG CGTGGAGTAC ATCCACCGGC AGGGCATCGT CCACCTGGAC
    CTCAAGCCCG AGAACATCAT GTGCGTCAAT AAGACCGGCA CGAGGATCAA GCTCATCGAC
    TTCGGCCTGG CCAGAAAGCT GGAGAATGCG GGGTCTCTGA AGGTCCTCTT TGGTACCCCG
    GAATTTGTGG CCCCCGAAGT GATCAACTAT GAGCCCATCG GCTATGCCAC GGACATGTGG
    AGCATCGGAG TCATCTGCTA CATCCTGGTT AGTGGACTCT CCCCCTTCAT GGGTGACAAC
    GATAATGAAA CCCTAGCCAA CGTTACCTCA GCCACCTGGG ACTTCGATGA TGAGGCGTTC
    GATGAGATCT CGGATGACGC CAAGGATTTT ATCAGCAATT TGCTGAAGAA AGATATGAAA
    AACCGCCTGG ACTGTACCCA GTGCCTTCAG CATCCCTGGC TCATGAAAGA CACCAAGAAC
    ATGGAAGCCA AGAAACTCTC CAAGGACCGG ATGAAGAAGT ACATGGCAAG AAGGAAATGG
    CAGAAAACGG GCAATGCTGT GAGAGCCATC GGAAGACTGT CCTCTATGGC AATGATCTCA
    GGGCTCAGTG GCAGGAAATC CTCAACAGGA TCACCGACCA GCCCGCTCAA CGCAGAAAAA
    CTAGAATCTG AAGAAGATGT GTCCCAAGCT TTCCTTGAGG CTGTTGCTGA GGAAAAGCCC
    CATGTAAAAC CCTATTTCTC TAAGACCATT CGTGATTTAG AAGTTGTGGA GGGAAGCGCT
    GCTAGATTTG ACTGCAAGAT TGAAGGATAC CCAGACCCTG AAGTTGTCTG GTTTAAAGAT
    GACCAGTCAA TCCGGGAGTC CCGCCACTTC CAGATAGACT ACGACGAGGA TGGGAACTGC
    TCTTTAATCA TTAGCGATGT TTGTGGGGAT GACGATGCCA AGTACACCTG CAAGGCTGTC
    AATAGTCTTG GAGAAGCCAC TTGCACAGCA GAGCTCATTG TGGAGACCAT GGAGGAAGGG
    GAAGGGGAAG GGGAAGAGGA AGAAGAGGAA GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44723
    Clone ID Related Accession (Same CDS sequence) XM_014110233.2 , XM_014110233.1
    Accession Version XM_014110233.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110233.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGGAGAAC TGCCCGGGGA GGAGGCCACG ACCATGGGGG ATGTGAAGCT GGCCGCCTTG 
    ACACAGGCTT CTAAAACCTC CGTCAGTGTG GATCACTCGA GGGCCGGGTC TGCACCCCCG
    ACAGAGGCCC CTGCTTTCAT TTTGCCCCCT CGGAACCTCT GCATCAGAGA AGGAGCCACC
    GCCAAGTTTG AAGGGAGGGT CCGGGGTTAC CCAGAGCCCC AGGTGACATG GCACCGAAAT
    GGGCAACCCA TCACCAGTGG GGGCCGCTTC CTGCTGGATT GTGGTATCCG GGGGACTTTC
    AGCCTCGTGA TTCATGCTGT CCGTGAGGAG GACCAGGGAA AGTATACCTG CGAAGCCACC
    AATGATGGTG GTGCCCGCCA GGTGACAGTG GAGTTGACTG TAGAAGGGGG CTTCATGAAG
    AAGCATGGTC AGCCCGTTGT TTCCAAAACC TTGGGGGACA GATTTGCAGC CCCCGCAGTG
    GAGACCCGTC CTAGCATCTG GGGAGAGTGC CCACCAAAGT TTGCTACCAA GTTGGGCCGA
    GCCGTAGTCA AAGAAGGACA GACGGGACGG TTCTCCTGCA AGATCACTGG ACGGCCCCAA
    CCACAGGTCA CCTGGCTCAA GGGGGATGTT GCCCTGCAGC CAAGTGCCCG TGTGTCTATG
    TCTGAGAAGA ACGGGGTACA AGTTCTGGAA ATCCATGAGG TCAGCCGCGA TGATGTGGGA
    GTGTACACAT GCATGGTGGT GAATGGGTCG GGGAAGGCCT CCATGTCGGC TGAGCTTTCT
    ATCCAAGGTT TGGACAATAC CAGTAGGTCA TTAACAAAGG TGGCCAATTC AGATATCAGG
    AAGGAGGTGA CCAATGGAAC CACGCAGGGG CCGAAACTAG ACCACCTGGA GTCTGCAGCG
    GAAAGGTGGC CCTGCTCCAG CACCCAGATC GGTGGCTCCC CCACCTGGGC CACAAGTAGC
    CAGCCTCAGC CCCTGAGGGA GTCCAAGCTG GAGCCGCCTG GGGACCCACC CAGGAAAGCC
    CTGAGGAGCC CCGTCCTACA GAAGACTTCC AGCACCATTA CCCTGCAGGC GGCAAAAGTC
    CAGCCAGAGC CAAGAGCGCC GGTCTCAAGT GCCCTCTCGT CTCCCGGAGA AGAGAGGGAG
    AGGCCAGCTG CTCCCCCCAC AGCCACCTTC CCTGCCCGGC AGTCTGGCCT GGGAAGCCAG
    GAGGTTGTGA ACAAGGTTGC TACCAGAAAA ATCCCCATGG AAAGCCAGAG AGATTCCACA
    TTCCCCAAAT TTGAGAGCAA GCCCCAAAGC CAGGAGGTCA GTGAAGATGA AACAGTCAAG
    TTCAGATGTG AGGTTTCAGG GACCCCAAAG CCTGAAGTGA CCTGGTTCCT GGAAGGTGCC
    CCCGTGAAGA GACGAGAAGG CATGGTTGAG GTTTATGAAG AAGGTGGATG CCACTACCTC
    TGCTTGCTGA GAGTGCGGGC CAAGGACAGT GGGAGCTACA GTTGCACGGC CTCCAATGTC
    CGAGGCCAGG TGTCCTGTGG TTGGACCCTC CTGGTGAAAC GGCTGGCCGT GATGGAGGTG
    GCTCCCTCGT TCTCCAGCGT CCTGAAGGAC TGCGCTGTCA TTGAGGGCCA GGACTTTGTG
    CTGCAGTGCT CAGTGCAGGG GACCCCGGTG CCCCAAATCA CTTGGCTGCT TAATGAGCAG
    CCCATCCAGT ACGCTCACTC CTCCTGCGAG GCTGGCGTGG CCGAGCTCCA CATCCAGGAT
    GCCCTGCCGG AAGATGATGG CATTTACACC TGTCTGGCCA AGAACACCTT GGGCCAGGTG
    TCCTGCAGCG CCCGGGTCAC CGTCCATGAA AAGAAGAGCG ACAGGAAGAG TGGGTATCTT
    CTGCGCACAG CTCCCGGCAA GCCCGTCGCA CCCCTCTTCC TGCAGGGCCT CTCTGACCTC
    AAAGTCATGG ATGGAAGCCA GGTCACCATG ACAGTCCAGG TGTCAGGGAA TCCGCCCCCC
    GAGGTCATCT GGCTTCACAA CGGGAATGAG ATCCAGGAGT CAGAGGACTT CCACTTTGAA
    CAGAGAGGCA CTCAGCACAG CCTTTGTATC CAGGAAGTGT TCCCAGAGGA CACAGGCACT
    TACACCTGCG AAGCCTGGAA CAGTGCTGGA GAGGTCCGCA CCCAGGCAGT ACTGATGGTG
    CAAGAGCCCC AGGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCCCGCTC ACTGACTGCC
    TCCCTGGGCC AGACTGTCCT CATCTCCTGT GCCATCGCTG GGGACCCCTT CCCCACTGTG
    CACTGGCTGC GAGATGGCAA AGCCCTCTCC AAAGACTCCG GCCACTTCGA GGTGCTACAG
    AACGAGGACG TGTTCACCCT GGTTCTAAAG AATGTGCAGC CCTGGCACGC CGGCCAGTAC
    GAGATCCAGC TCAAGAACCG GGTTGGCGAA TGCAGTTGCC AGGTGTCACT GATGCTACAG
    AGCAGCCCCA CCGGAGCCCC CCTGCGGGGG AGGGAGCCTG CCAGCTGCGA GGGCCTCTGC
    AGCCGGGGAG CCGGCGCTGA TGGTGGTGGC GGTGACTGCT ATGGGACCCT GAGGCCTGGC
    CGGCCAGCGA GAGGACAGGG TTGGCCAGAG GAGGAGGACG ACGGCGAGGA CGTGCGGGGG
    GTGCTGAAGA GGCGCGTGGA GACCCGGCAA CATACTGAGG AGGCCATCCG CCAGCAGCAG
    GTGGAACAGC TGGACTTCCG TGACCTCCTG GGGAAGAAGG TGAGTACCAA GACCGTGTCA
    GAAGAAGACC TGAAGGAGAT CCCAGCGGAG CAGATGGATT TCCGCGCCAA CCTGCAGCGG
    CAAGTGAAGC CAAAGACTGT GTCAGAGGAA GAGAGGAAGG TGCATAGCCC TCAGCAGGTC
    GACTTCCGCT CGGTCCTGGC CAAAAAGGGG ACTCCTAAGA CCCCGGTGCC TGAGAAGGCA
    CCTCTTCCCA AACCCGCCAC CCCAGATTTC CGCTCCGTGT TAGGTAGCAA GAAAAAATTA
    CCAGCCGAGA ATGGTAGCAA CAACGCTGAG GCCTTGAATG CCAAGGCAGC AGAAAGTCCC
    AAGGCTGTGA GCAATGCCCA GCCTTTAGGG TCCCTGAAAC CTTTGGGCAA TGCCAAGCCT
    GCTGAGACTC TAAAACCTGT GGGCAACATC AGGCCTGCTG AGACGCTGAA ACCCATAGGC
    AACGCCAAAC CTGCTGAGAC CCTGAAACCC ATGGGCAACG CCAAGCCTGC TGAGACGCTA
    AAACCCATAG GCAATGCCAA GCTTGCTGAG ACCCCGAAAC CCATGGGCAA TGCCAAGCCT
    GCTGAGACCC TGAAACCCCT GGGCAACGCC AAGCCTGCTG AGACGCTGAA ACCCATAAGC
    AACGCCAAGC CTGCTGAACC TCTGAAACCT GTGGGCAACG CCAAGCCTGC TGAGACCCCA
    AAACCCATAG GTAATGCCAA GCCTGCTGAG ATCCTAAAAC CAGCTGGGAA GGAAGAACTC
    AAGAAGGAGG TTAAGAATGA CGTGAACTGC AAGAGGGCAC AGGCAGGAGC CACAGATAAT
    GAAAAAAGAT CAGAGAACCA AGGGACAGCC CCAGCCTTCA AGGAGAAGCT GCAAGATGTC
    CATGTGACAG AGGGTGAGAA GCTGCTACTC CAGTGTCAGG TGTCGTCTGA CCCTCCAGCC
    ACCATCACCT GGATGCTCAA TGGAAAGACA CTCAAGACCA CCAAGTTCAT CATCCTCTCC
    CAAGAAGGCT CACTGTGCTC CGTGTCCATC GAGAAGGCCC TGCCCGAGGA CAGAGGCTTC
    TACAAGTGCA TAGCTAAGAA TGGCGCCGGC CAGGCTGAGT GCTCCTGCCA AGTCACCGTG
    GACGATGCTC CCACCAAGGA GAATGCCAAG GCCCCGGAGA TGAAAGCCCG GAGGCCCAAG
    AGCTCTCTAC CTCCCATGCT AGGAACAGAG AGTGATGCAA CTGTGAAAAA GAAACCTGCC
    CCCAAGACAC CTCCAAAAGC AGCCATGCCC CCTCAGATCA TCCAGTTTCC TGAGGACCAA
    AAGGTGCGCG CAGGAGAGTC TGTGGAGCTG TTTGGTAAAG TGGCAGGCAC CCAGCCCATC
    ACCTGTACGT GGATGAAGTT CCGAAAGCAG ATCCAGGAGA GCGAGCACAT CAAGGTGGAG
    ACCAGTGAGA ATGGCAGCAG GCTCACCATC GTGGCCGCAC GCCAGGAACA CTGCGGCTGC
    TACACTCTGC TGGTGGAGAA CAAGCTGGGC AGCAGGCAGG CCCAGGTCAA CCTCACTGTC
    GTGGACAAAC CAGACCCCCC AGCGGGCACA CCTTGCGCCT CCGACATCCG GAGCTCGTCG
    TTGACCCTGT CCTGGTATGG CTCCTCGTAT GATGGGGGCA GTGCCGTGCA ATCCTACAGC
    GTCGAGATCT GGGACTCGGT GGACAAGACG TGGAAGGAAC TAGCCACGTG CCGCAGCACC
    TCTTTTAACG TCCAGGACCT GCTGCCTGAC CGAGAGTATA AATTCCGCGT TCGCGCCATC
    AACGTGTATG GAACCAGTGA GCCAAGCCAG GAGTCTGAAC TCACAGCAGT GGGAGAGAAA
    CCGGAGGAGC CGAAGGACGA AGTGGAGGTG TCGGATGATG ATGAGAAGGA ACCTGAGGTC
    GACTACCGGA CAGTGACGGT CAACACTGAA CAAAAAGTGT CCGACTTCTA TGACATCGAA
    GAGAGACTAG GCTCTGGGAA ATTTGGACAG GTCTTCCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCA TATTCAGCCA AAGAGAAGGA GAATATCCGG
    CAGGAGATTA GCATCATGAA CTGCCTCCAC CACCCCAAGC TGGTCCAGTG TGTGGATGCC
    TTCGAAGAAA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCGGGGGG CGAGCTGTTC
    GAGCGCATCA TCGACGAGGA CTTCGAGCTG ACGGAGCGCG AGTGCATCCA GTACATGAGG
    CAGATCTCGG CGGGCGTGGA GTACATCCAC CGGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGCGT CAATAAGACC GGCACGAGGA TCAAGCTCAT CGACTTCGGC
    CTGGCCAGAA AGCTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGTAC CCCGGAATTT
    GTGGCCCCCG AAGTGATCAA CTATGAGCCC ATCGGCTATG CCACGGACAT GTGGAGCATC
    GGAGTCATCT GCTACATCCT GGTTAGTGGA CTCTCCCCCT TCATGGGTGA CAACGATAAT
    GAAACCCTAG CCAACGTTAC CTCAGCCACC TGGGACTTCG ATGATGAGGC GTTCGATGAG
    ATCTCGGATG ACGCCAAGGA TTTTATCAGC AATTTGCTGA AGAAAGATAT GAAAAACCGC
    CTGGACTGTA CCCAGTGCCT TCAGCATCCC TGGCTCATGA AAGACACCAA GAACATGGAA
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGATCACCG ACCAGCCCGC TCAACGCAGA AAAACTAGAA
    TCTGAAGAAG ATGTGTCCCA AGCTTTCCTT GAGGCTGTTG CTGAGGAAAA GCCCCATGTA
    AAACCCTATT TCTCTAAGAC CATTCGTGAT TTAGAAGTTG TGGAGGGAAG CGCTGCTAGA
    TTTGACTGCA AGATTGAAGG ATACCCAGAC CCTGAAGTTG TCTGGTTTAA AGATGACCAG
    TCAATCCGGG AGTCCCGCCA CTTCCAGATA GACTACGACG AGGATGGGAA CTGCTCTTTA
    ATCATTAGCG ATGTTTGTGG GGATGACGAT GCCAAGTACA CCTGCAAGGC TGTCAATAGT
    CTTGGAGAAG CCACTTGCAC AGCAGAGCTC ATTGTGGAGA CCATGGAGGA AGGGGAAGGG
    GAAGGGGAAG AGGAAGAAGA GGAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965708.1
    CDS92..6121
    Translation

    Target ORF information:

    RefSeq Version XM_014110233.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110233.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGGAGAAC TGCCCGGGGA GGAGGCCACG ACCATGGGGG ATGTGAAGCT GGCCGCCTTG 
    ACACAGGCTT CTAAAACCTC CGTCAGTGTG GATCACTCGA GGGCCGGGTC TGCACCCCCG
    ACAGAGGCCC CTGCTTTCAT TTTGCCCCCT CGGAACCTCT GCATCAGAGA AGGAGCCACC
    GCCAAGTTTG AAGGGAGGGT CCGGGGTTAC CCAGAGCCCC AGGTGACATG GCACCGAAAT
    GGGCAACCCA TCACCAGTGG GGGCCGCTTC CTGCTGGATT GTGGTATCCG GGGGACTTTC
    AGCCTCGTGA TTCATGCTGT CCGTGAGGAG GACCAGGGAA AGTATACCTG CGAAGCCACC
    AATGATGGTG GTGCCCGCCA GGTGACAGTG GAGTTGACTG TAGAAGGGGG CTTCATGAAG
    AAGCATGGTC AGCCCGTTGT TTCCAAAACC TTGGGGGACA GATTTGCAGC CCCCGCAGTG
    GAGACCCGTC CTAGCATCTG GGGAGAGTGC CCACCAAAGT TTGCTACCAA GTTGGGCCGA
    GCCGTAGTCA AAGAAGGACA GACGGGACGG TTCTCCTGCA AGATCACTGG ACGGCCCCAA
    CCACAGGTCA CCTGGCTCAA GGGGGATGTT GCCCTGCAGC CAAGTGCCCG TGTGTCTATG
    TCTGAGAAGA ACGGGGTACA AGTTCTGGAA ATCCATGAGG TCAGCCGCGA TGATGTGGGA
    GTGTACACAT GCATGGTGGT GAATGGGTCG GGGAAGGCCT CCATGTCGGC TGAGCTTTCT
    ATCCAAGGTT TGGACAATAC CAGTAGGTCA TTAACAAAGG TGGCCAATTC AGATATCAGG
    AAGGAGGTGA CCAATGGAAC CACGCAGGGG CCGAAACTAG ACCACCTGGA GTCTGCAGCG
    GAAAGGTGGC CCTGCTCCAG CACCCAGATC GGTGGCTCCC CCACCTGGGC CACAAGTAGC
    CAGCCTCAGC CCCTGAGGGA GTCCAAGCTG GAGCCGCCTG GGGACCCACC CAGGAAAGCC
    CTGAGGAGCC CCGTCCTACA GAAGACTTCC AGCACCATTA CCCTGCAGGC GGCAAAAGTC
    CAGCCAGAGC CAAGAGCGCC GGTCTCAAGT GCCCTCTCGT CTCCCGGAGA AGAGAGGGAG
    AGGCCAGCTG CTCCCCCCAC AGCCACCTTC CCTGCCCGGC AGTCTGGCCT GGGAAGCCAG
    GAGGTTGTGA ACAAGGTTGC TACCAGAAAA ATCCCCATGG AAAGCCAGAG AGATTCCACA
    TTCCCCAAAT TTGAGAGCAA GCCCCAAAGC CAGGAGGTCA GTGAAGATGA AACAGTCAAG
    TTCAGATGTG AGGTTTCAGG GACCCCAAAG CCTGAAGTGA CCTGGTTCCT GGAAGGTGCC
    CCCGTGAAGA GACGAGAAGG CATGGTTGAG GTTTATGAAG AAGGTGGATG CCACTACCTC
    TGCTTGCTGA GAGTGCGGGC CAAGGACAGT GGGAGCTACA GTTGCACGGC CTCCAATGTC
    CGAGGCCAGG TGTCCTGTGG TTGGACCCTC CTGGTGAAAC GGCTGGCCGT GATGGAGGTG
    GCTCCCTCGT TCTCCAGCGT CCTGAAGGAC TGCGCTGTCA TTGAGGGCCA GGACTTTGTG
    CTGCAGTGCT CAGTGCAGGG GACCCCGGTG CCCCAAATCA CTTGGCTGCT TAATGAGCAG
    CCCATCCAGT ACGCTCACTC CTCCTGCGAG GCTGGCGTGG CCGAGCTCCA CATCCAGGAT
    GCCCTGCCGG AAGATGATGG CATTTACACC TGTCTGGCCA AGAACACCTT GGGCCAGGTG
    TCCTGCAGCG CCCGGGTCAC CGTCCATGAA AAGAAGAGCG ACAGGAAGAG TGGGTATCTT
    CTGCGCACAG CTCCCGGCAA GCCCGTCGCA CCCCTCTTCC TGCAGGGCCT CTCTGACCTC
    AAAGTCATGG ATGGAAGCCA GGTCACCATG ACAGTCCAGG TGTCAGGGAA TCCGCCCCCC
    GAGGTCATCT GGCTTCACAA CGGGAATGAG ATCCAGGAGT CAGAGGACTT CCACTTTGAA
    CAGAGAGGCA CTCAGCACAG CCTTTGTATC CAGGAAGTGT TCCCAGAGGA CACAGGCACT
    TACACCTGCG AAGCCTGGAA CAGTGCTGGA GAGGTCCGCA CCCAGGCAGT ACTGATGGTG
    CAAGAGCCCC AGGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCCCGCTC ACTGACTGCC
    TCCCTGGGCC AGACTGTCCT CATCTCCTGT GCCATCGCTG GGGACCCCTT CCCCACTGTG
    CACTGGCTGC GAGATGGCAA AGCCCTCTCC AAAGACTCCG GCCACTTCGA GGTGCTACAG
    AACGAGGACG TGTTCACCCT GGTTCTAAAG AATGTGCAGC CCTGGCACGC CGGCCAGTAC
    GAGATCCAGC TCAAGAACCG GGTTGGCGAA TGCAGTTGCC AGGTGTCACT GATGCTACAG
    AGCAGCCCCA CCGGAGCCCC CCTGCGGGGG AGGGAGCCTG CCAGCTGCGA GGGCCTCTGC
    AGCCGGGGAG CCGGCGCTGA TGGTGGTGGC GGTGACTGCT ATGGGACCCT GAGGCCTGGC
    CGGCCAGCGA GAGGACAGGG TTGGCCAGAG GAGGAGGACG ACGGCGAGGA CGTGCGGGGG
    GTGCTGAAGA GGCGCGTGGA GACCCGGCAA CATACTGAGG AGGCCATCCG CCAGCAGCAG
    GTGGAACAGC TGGACTTCCG TGACCTCCTG GGGAAGAAGG TGAGTACCAA GACCGTGTCA
    GAAGAAGACC TGAAGGAGAT CCCAGCGGAG CAGATGGATT TCCGCGCCAA CCTGCAGCGG
    CAAGTGAAGC CAAAGACTGT GTCAGAGGAA GAGAGGAAGG TGCATAGCCC TCAGCAGGTC
    GACTTCCGCT CGGTCCTGGC CAAAAAGGGG ACTCCTAAGA CCCCGGTGCC TGAGAAGGCA
    CCTCTTCCCA AACCCGCCAC CCCAGATTTC CGCTCCGTGT TAGGTAGCAA GAAAAAATTA
    CCAGCCGAGA ATGGTAGCAA CAACGCTGAG GCCTTGAATG CCAAGGCAGC AGAAAGTCCC
    AAGGCTGTGA GCAATGCCCA GCCTTTAGGG TCCCTGAAAC CTTTGGGCAA TGCCAAGCCT
    GCTGAGACTC TAAAACCTGT GGGCAACATC AGGCCTGCTG AGACGCTGAA ACCCATAGGC
    AACGCCAAAC CTGCTGAGAC CCTGAAACCC ATGGGCAACG CCAAGCCTGC TGAGACGCTA
    AAACCCATAG GCAATGCCAA GCTTGCTGAG ACCCCGAAAC CCATGGGCAA TGCCAAGCCT
    GCTGAGACCC TGAAACCCCT GGGCAACGCC AAGCCTGCTG AGACGCTGAA ACCCATAAGC
    AACGCCAAGC CTGCTGAACC TCTGAAACCT GTGGGCAACG CCAAGCCTGC TGAGACCCCA
    AAACCCATAG GTAATGCCAA GCCTGCTGAG ATCCTAAAAC CAGCTGGGAA GGAAGAACTC
    AAGAAGGAGG TTAAGAATGA CGTGAACTGC AAGAGGGCAC AGGCAGGAGC CACAGATAAT
    GAAAAAAGAT CAGAGAACCA AGGGACAGCC CCAGCCTTCA AGGAGAAGCT GCAAGATGTC
    CATGTGACAG AGGGTGAGAA GCTGCTACTC CAGTGTCAGG TGTCGTCTGA CCCTCCAGCC
    ACCATCACCT GGATGCTCAA TGGAAAGACA CTCAAGACCA CCAAGTTCAT CATCCTCTCC
    CAAGAAGGCT CACTGTGCTC CGTGTCCATC GAGAAGGCCC TGCCCGAGGA CAGAGGCTTC
    TACAAGTGCA TAGCTAAGAA TGGCGCCGGC CAGGCTGAGT GCTCCTGCCA AGTCACCGTG
    GACGATGCTC CCACCAAGGA GAATGCCAAG GCCCCGGAGA TGAAAGCCCG GAGGCCCAAG
    AGCTCTCTAC CTCCCATGCT AGGAACAGAG AGTGATGCAA CTGTGAAAAA GAAACCTGCC
    CCCAAGACAC CTCCAAAAGC AGCCATGCCC CCTCAGATCA TCCAGTTTCC TGAGGACCAA
    AAGGTGCGCG CAGGAGAGTC TGTGGAGCTG TTTGGTAAAG TGGCAGGCAC CCAGCCCATC
    ACCTGTACGT GGATGAAGTT CCGAAAGCAG ATCCAGGAGA GCGAGCACAT CAAGGTGGAG
    ACCAGTGAGA ATGGCAGCAG GCTCACCATC GTGGCCGCAC GCCAGGAACA CTGCGGCTGC
    TACACTCTGC TGGTGGAGAA CAAGCTGGGC AGCAGGCAGG CCCAGGTCAA CCTCACTGTC
    GTGGACAAAC CAGACCCCCC AGCGGGCACA CCTTGCGCCT CCGACATCCG GAGCTCGTCG
    TTGACCCTGT CCTGGTATGG CTCCTCGTAT GATGGGGGCA GTGCCGTGCA ATCCTACAGC
    GTCGAGATCT GGGACTCGGT GGACAAGACG TGGAAGGAAC TAGCCACGTG CCGCAGCACC
    TCTTTTAACG TCCAGGACCT GCTGCCTGAC CGAGAGTATA AATTCCGCGT TCGCGCCATC
    AACGTGTATG GAACCAGTGA GCCAAGCCAG GAGTCTGAAC TCACAGCAGT GGGAGAGAAA
    CCGGAGGAGC CGAAGGACGA AGTGGAGGTG TCGGATGATG ATGAGAAGGA ACCTGAGGTC
    GACTACCGGA CAGTGACGGT CAACACTGAA CAAAAAGTGT CCGACTTCTA TGACATCGAA
    GAGAGACTAG GCTCTGGGAA ATTTGGACAG GTCTTCCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCA TATTCAGCCA AAGAGAAGGA GAATATCCGG
    CAGGAGATTA GCATCATGAA CTGCCTCCAC CACCCCAAGC TGGTCCAGTG TGTGGATGCC
    TTCGAAGAAA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCGGGGGG CGAGCTGTTC
    GAGCGCATCA TCGACGAGGA CTTCGAGCTG ACGGAGCGCG AGTGCATCCA GTACATGAGG
    CAGATCTCGG CGGGCGTGGA GTACATCCAC CGGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGCGT CAATAAGACC GGCACGAGGA TCAAGCTCAT CGACTTCGGC
    CTGGCCAGAA AGCTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGTAC CCCGGAATTT
    GTGGCCCCCG AAGTGATCAA CTATGAGCCC ATCGGCTATG CCACGGACAT GTGGAGCATC
    GGAGTCATCT GCTACATCCT GGTTAGTGGA CTCTCCCCCT TCATGGGTGA CAACGATAAT
    GAAACCCTAG CCAACGTTAC CTCAGCCACC TGGGACTTCG ATGATGAGGC GTTCGATGAG
    ATCTCGGATG ACGCCAAGGA TTTTATCAGC AATTTGCTGA AGAAAGATAT GAAAAACCGC
    CTGGACTGTA CCCAGTGCCT TCAGCATCCC TGGCTCATGA AAGACACCAA GAACATGGAA
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGATCACCG ACCAGCCCGC TCAACGCAGA AAAACTAGAA
    TCTGAAGAAG ATGTGTCCCA AGCTTTCCTT GAGGCTGTTG CTGAGGAAAA GCCCCATGTA
    AAACCCTATT TCTCTAAGAC CATTCGTGAT TTAGAAGTTG TGGAGGGAAG CGCTGCTAGA
    TTTGACTGCA AGATTGAAGG ATACCCAGAC CCTGAAGTTG TCTGGTTTAA AGATGACCAG
    TCAATCCGGG AGTCCCGCCA CTTCCAGATA GACTACGACG AGGATGGGAA CTGCTCTTTA
    ATCATTAGCG ATGTTTGTGG GGATGACGAT GCCAAGTACA CCTGCAAGGC TGTCAATAGT
    CTTGGAGAAG CCACTTGCAC AGCAGAGCTC ATTGTGGAGA CCATGGAGGA AGGGGAAGGG
    GAAGGGGAAG AGGAAGAAGA GGAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44723
    Clone ID Related Accession (Same CDS sequence) XM_014110233.2 , XM_014110233.1
    Accession Version XM_014110233.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726118.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGGAGAAC TGCCCGGGGA GGAGGCCACG ACCATGGGGG ATGTGAAGCT GGCCGCCTTG 
    ACACAGGCTT CTAAAACCTC CGTCAGTGTG GATCACTCGA GGGCCGGGTC TGCACCCCCG
    ACAGAGGCCC CTGCTTTCAT TTTGCCCCCT CGGAACCTCT GCATCAGAGA AGGAGCCACC
    GCCAAGTTTG AAGGGAGGGT CCGGGGTTAC CCAGAGCCCC AGGTGACATG GCACCGAAAT
    GGGCAACCCA TCACCAGTGG GGGCCGCTTC CTGCTGGATT GTGGTATCCG GGGGACTTTC
    AGCCTCGTGA TTCATGCTGT CCGTGAGGAG GACCAGGGAA AGTATACCTG CGAAGCCACC
    AATGATGGTG GTGCCCGCCA GGTGACAGTG GAGTTGACTG TAGAAGGGGG CTTCATGAAG
    AAGCATGGTC AGCCCGTTGT TTCCAAAACC TTGGGGGACA GATTTGCAGC CCCCGCAGTG
    GAGACCCGTC CTAGCATCTG GGGAGAGTGC CCACCAAAGT TTGCTACCAA GTTGGGCCGA
    GCCGTAGTCA AAGAAGGACA GACGGGACGG TTCTCCTGCA AGATCACTGG ACGGCCCCAA
    CCACAGGTCA CCTGGCTCAA GGGGGATGTT GCCCTGCAGC CAAGTGCCCG TGTGTCTATG
    TCTGAGAAGA ACGGGGTACA AGTTCTGGAA ATCCATGAGG TCAGCCGCGA TGATGTGGGA
    GTGTACACAT GCATGGTGGT GAATGGGTCG GGGAAGGCCT CCATGTCGGC TGAGCTTTCT
    ATCCAAGGTT TGGACAATAC CAGTAGGTCA TTAACAAAGG TGGCCAATTC AGATATCAGG
    AAGGAGGTGA CCAATGGAAC CACGCAGGGG CCGAAACTAG ACCACCTGGA GTCTGCAGCG
    GAAAGGTGGC CCTGCTCCAG CACCCAGATC GGTGGCTCCC CCACCTGGGC CACAAGTAGC
    CAGCCTCAGC CCCTGAGGGA GTCCAAGCTG GAGCCGCCTG GGGACCCACC CAGGAAAGCC
    CTGAGGAGCC CCGTCCTACA GAAGACTTCC AGCACCATTA CCCTGCAGGC GGCAAAAGTC
    CAGCCAGAGC CAAGAGCGCC GGTCTCAAGT GCCCTCTCGT CTCCCGGAGA AGAGAGGGAG
    AGGCCAGCTG CTCCCCCCAC AGCCACCTTC CCTGCCCGGC AGTCTGGCCT GGGAAGCCAG
    GAGGTTGTGA ACAAGGTTGC TACCAGAAAA ATCCCCATGG AAAGCCAGAG AGATTCCACA
    TTCCCCAAAT TTGAGAGCAA GCCCCAAAGC CAGGAGGTCA GTGAAGATGA AACAGTCAAG
    TTCAGATGTG AGGTTTCAGG GACCCCAAAG CCTGAAGTGA CCTGGTTCCT GGAAGGTGCC
    CCCGTGAAGA GACGAGAAGG CATGGTTGAG GTTTATGAAG AAGGTGGATG CCACTACCTC
    TGCTTGCTGA GAGTGCGGGC CAAGGACAGT GGGAGCTACA GTTGCACGGC CTCCAATGTC
    CGAGGCCAGG TGTCCTGTGG TTGGACCCTC CTGGTGAAAC GGCTGGCCGT GATGGAGGTG
    GCTCCCTCGT TCTCCAGCGT CCTGAAGGAC TGCGCTGTCA TTGAGGGCCA GGACTTTGTG
    CTGCAGTGCT CAGTGCAGGG GACCCCGGTG CCCCAAATCA CTTGGCTGCT TAATGAGCAG
    CCCATCCAGT ACGCTCACTC CTCCTGCGAG GCTGGCGTGG CCGAGCTCCA CATCCAGGAT
    GCCCTGCCGG AAGATGATGG CATTTACACC TGTCTGGCCA AGAACACCTT GGGCCAGGTG
    TCCTGCAGCG CCCGGGTCAC CGTCCATGAA AAGAAGAGCG ACAGGAAGAG TGGGTATCTT
    CTGCGCACAG CTCCCGGCAA GCCCGTCGCA CCCCTCTTCC TGCAGGGCCT CTCTGACCTC
    AAAGTCATGG ATGGAAGCCA GGTCACCATG ACAGTCCAGG TGTCAGGGAA TCCGCCCCCC
    GAGGTCATCT GGCTTCACAA CGGGAATGAG ATCCAGGAGT CAGAGGACTT CCACTTTGAA
    CAGAGAGGCA CTCAGCACAG CCTTTGTATC CAGGAAGTGT TCCCAGAGGA CACAGGCACT
    TACACCTGCG AAGCCTGGAA CAGTGCTGGA GAGGTCCGCA CCCAGGCAGT ACTGATGGTG
    CAAGAGCCCC AGGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCCCGCTC ACTGACTGCC
    TCCCTGGGCC AGACTGTCCT CATCTCCTGT GCCATCGCTG GGGACCCCTT CCCCACTGTG
    CACTGGCTGC GAGATGGCAA AGCCCTCTCC AAAGACTCCG GCCACTTCGA GGTGCTACAG
    AACGAGGACG TGTTCACCCT GGTTCTAAAG AATGTGCAGC CCTGGCACGC CGGCCAGTAC
    GAGATCCAGC TCAAGAACCG GGTTGGCGAA TGCAGTTGCC AGGTGTCACT GATGCTACAG
    AGCAGCCCCA CCGGAGCCCC CCTGCGGGGG AGGGAGCCTG CCAGCTGCGA GGGCCTCTGC
    AGCCGGGGAG CCGGCGCTGA TGGTGGTGGC GGTGACTGCT ATGGGACCCT GAGGCCTGGC
    CGGCCAGCGA GAGGACAGGG TTGGCCAGAG GAGGAGGACG ACGGCGAGGA CGTGCGGGGG
    GTGCTGAAGA GGCGCGTGGA GACCCGGCAA CATACTGAGG AGGCCATCCG CCAGCAGCAG
    GTGGAACAGC TGGACTTCCG TGACCTCCTG GGGAAGAAGG TGAGTACCAA GACCGTGTCA
    GAAGAAGACC TGAAGGAGAT CCCAGCGGAG CAGATGGATT TCCGCGCCAA CCTGCAGCGG
    CAAGTGAAGC CAAAGACTGT GTCAGAGGAA GAGAGGAAGG TGCATAGCCC TCAGCAGGTC
    GACTTCCGCT CGGTCCTGGC CAAAAAGGGG ACTCCTAAGA CCCCGGTGCC TGAGAAGGCA
    CCTCTTCCCA AACCCGCCAC CCCAGATTTC CGCTCCGTGT TAGGTAGCAA GAAAAAATTA
    CCAGCCGAGA ATGGTAGCAA CAACGCTGAG GCCTTGAATG CCAAGGCAGC AGAAAGTCCC
    AAGGCTGTGA GCAATGCCCA GCCTTTAGGG TCCCTGAAAC CTTTGGGCAA TGCCAAGCCT
    GCTGAGACTC TAAAACCTGT GGGCAACATC AGGCCTGCTG AGACGCTGAA ACCCATAGGC
    AACGCCAAAC CTGCTGAGAC CCTGAAACCC ATGGGCAACG CCAAGCCTGC TGAGACGCTA
    AAACCCATAG GCAATGCCAA GCTTGCTGAG ACCCCGAAAC CCATGGGCAA TGCCAAGCCT
    GCTGAGACCC TGAAACCCCT GGGCAACGCC AAGCCTGCTG AGACGCTGAA ACCCATAAGC
    AACGCCAAGC CTGCTGAACC TCTGAAACCT GTGGGCAACG CCAAGCCTGC TGAGACCCCA
    AAACCCATAG GTAATGCCAA GCCTGCTGAG ATCCTAAAAC CAGCTGGGAA GGAAGAACTC
    AAGAAGGAGG TTAAGAATGA CGTGAACTGC AAGAGGGCAC AGGCAGGAGC CACAGATAAT
    GAAAAAAGAT CAGAGAACCA AGGGACAGCC CCAGCCTTCA AGGAGAAGCT GCAAGATGTC
    CATGTGACAG AGGGTGAGAA GCTGCTACTC CAGTGTCAGG TGTCGTCTGA CCCTCCAGCC
    ACCATCACCT GGATGCTCAA TGGAAAGACA CTCAAGACCA CCAAGTTCAT CATCCTCTCC
    CAAGAAGGCT CACTGTGCTC CGTGTCCATC GAGAAGGCCC TGCCCGAGGA CAGAGGCTTC
    TACAAGTGCA TAGCTAAGAA TGGCGCCGGC CAGGCTGAGT GCTCCTGCCA AGTCACCGTG
    GACGATGCTC CCACCAAGGA GAATGCCAAG GCCCCGGAGA TGAAAGCCCG GAGGCCCAAG
    AGCTCTCTAC CTCCCATGCT AGGAACAGAG AGTGATGCAA CTGTGAAAAA GAAACCTGCC
    CCCAAGACAC CTCCAAAAGC AGCCATGCCC CCTCAGATCA TCCAGTTTCC TGAGGACCAA
    AAGGTGCGCG CAGGAGAGTC TGTGGAGCTG TTTGGTAAAG TGGCAGGCAC CCAGCCCATC
    ACCTGTACGT GGATGAAGTT CCGAAAGCAG ATCCAGGAGA GCGAGCACAT CAAGGTGGAG
    ACCAGTGAGA ATGGCAGCAG GCTCACCATC GTGGCCGCAC GCCAGGAACA CTGCGGCTGC
    TACACTCTGC TGGTGGAGAA CAAGCTGGGC AGCAGGCAGG CCCAGGTCAA CCTCACTGTC
    GTGGACAAAC CAGACCCCCC AGCGGGCACA CCTTGCGCCT CCGACATCCG GAGCTCGTCG
    TTGACCCTGT CCTGGTATGG CTCCTCGTAT GATGGGGGCA GTGCCGTGCA ATCCTACAGC
    GTCGAGATCT GGGACTCGGT GGACAAGACG TGGAAGGAAC TAGCCACGTG CCGCAGCACC
    TCTTTTAACG TCCAGGACCT GCTGCCTGAC CGAGAGTATA AATTCCGCGT TCGCGCCATC
    AACGTGTATG GAACCAGTGA GCCAAGCCAG GAGTCTGAAC TCACAGCAGT GGGAGAGAAA
    CCGGAGGAGC CGAAGGACGA AGTGGAGGTG TCGGATGATG ATGAGAAGGA ACCTGAGGTC
    GACTACCGGA CAGTGACGGT CAACACTGAA CAAAAAGTGT CCGACTTCTA TGACATCGAA
    GAGAGACTAG GCTCTGGGAA ATTTGGACAG GTCTTCCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCA TATTCAGCCA AAGAGAAGGA GAATATCCGG
    CAGGAGATTA GCATCATGAA CTGCCTCCAC CACCCCAAGC TGGTCCAGTG TGTGGATGCC
    TTCGAAGAAA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCGGGGGG CGAGCTGTTC
    GAGCGCATCA TCGACGAGGA CTTCGAGCTG ACGGAGCGCG AGTGCATCCA GTACATGAGG
    CAGATCTCGG CGGGCGTGGA GTACATCCAC CGGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGCGT CAATAAGACC GGCACGAGGA TCAAGCTCAT CGACTTCGGC
    CTGGCCAGAA AGCTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGTAC CCCGGAATTT
    GTGGCCCCCG AAGTGATCAA CTATGAGCCC ATCGGCTATG CCACGGACAT GTGGAGCATC
    GGAGTCATCT GCTACATCCT GGTTAGTGGA CTCTCCCCCT TCATGGGTGA CAACGATAAT
    GAAACCCTAG CCAACGTTAC CTCAGCCACC TGGGACTTCG ATGATGAGGC GTTCGATGAG
    ATCTCGGATG ACGCCAAGGA TTTTATCAGC AATTTGCTGA AGAAAGATAT GAAAAACCGC
    CTGGACTGTA CCCAGTGCCT TCAGCATCCC TGGCTCATGA AAGACACCAA GAACATGGAA
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGATCACCG ACCAGCCCGC TCAACGCAGA AAAACTAGAA
    TCTGAAGAAG ATGTGTCCCA AGCTTTCCTT GAGGCTGTTG CTGAGGAAAA GCCCCATGTA
    AAACCCTATT TCTCTAAGAC CATTCGTGAT TTAGAAGTTG TGGAGGGAAG CGCTGCTAGA
    TTTGACTGCA AGATTGAAGG ATACCCAGAC CCTGAAGTTG TCTGGTTTAA AGATGACCAG
    TCAATCCGGG AGTCCCGCCA CTTCCAGATA GACTACGACG AGGATGGGAA CTGCTCTTTA
    ATCATTAGCG ATGTTTGTGG GGATGACGAT GCCAAGTACA CCTGCAAGGC TGTCAATAGT
    CTTGGAGAAG CCACTTGCAC AGCAGAGCTC ATTGTGGAGA CCATGGAGGA AGGGGAAGGG
    GAAGGGGAAG AGGAAGAAGA GGAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965708.1
    CDS265..6294
    Translation

    Target ORF information:

    RefSeq Version XM_014110233.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110233.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGGAGAAC TGCCCGGGGA GGAGGCCACG ACCATGGGGG ATGTGAAGCT GGCCGCCTTG 
    ACACAGGCTT CTAAAACCTC CGTCAGTGTG GATCACTCGA GGGCCGGGTC TGCACCCCCG
    ACAGAGGCCC CTGCTTTCAT TTTGCCCCCT CGGAACCTCT GCATCAGAGA AGGAGCCACC
    GCCAAGTTTG AAGGGAGGGT CCGGGGTTAC CCAGAGCCCC AGGTGACATG GCACCGAAAT
    GGGCAACCCA TCACCAGTGG GGGCCGCTTC CTGCTGGATT GTGGTATCCG GGGGACTTTC
    AGCCTCGTGA TTCATGCTGT CCGTGAGGAG GACCAGGGAA AGTATACCTG CGAAGCCACC
    AATGATGGTG GTGCCCGCCA GGTGACAGTG GAGTTGACTG TAGAAGGGGG CTTCATGAAG
    AAGCATGGTC AGCCCGTTGT TTCCAAAACC TTGGGGGACA GATTTGCAGC CCCCGCAGTG
    GAGACCCGTC CTAGCATCTG GGGAGAGTGC CCACCAAAGT TTGCTACCAA GTTGGGCCGA
    GCCGTAGTCA AAGAAGGACA GACGGGACGG TTCTCCTGCA AGATCACTGG ACGGCCCCAA
    CCACAGGTCA CCTGGCTCAA GGGGGATGTT GCCCTGCAGC CAAGTGCCCG TGTGTCTATG
    TCTGAGAAGA ACGGGGTACA AGTTCTGGAA ATCCATGAGG TCAGCCGCGA TGATGTGGGA
    GTGTACACAT GCATGGTGGT GAATGGGTCG GGGAAGGCCT CCATGTCGGC TGAGCTTTCT
    ATCCAAGGTT TGGACAATAC CAGTAGGTCA TTAACAAAGG TGGCCAATTC AGATATCAGG
    AAGGAGGTGA CCAATGGAAC CACGCAGGGG CCGAAACTAG ACCACCTGGA GTCTGCAGCG
    GAAAGGTGGC CCTGCTCCAG CACCCAGATC GGTGGCTCCC CCACCTGGGC CACAAGTAGC
    CAGCCTCAGC CCCTGAGGGA GTCCAAGCTG GAGCCGCCTG GGGACCCACC CAGGAAAGCC
    CTGAGGAGCC CCGTCCTACA GAAGACTTCC AGCACCATTA CCCTGCAGGC GGCAAAAGTC
    CAGCCAGAGC CAAGAGCGCC GGTCTCAAGT GCCCTCTCGT CTCCCGGAGA AGAGAGGGAG
    AGGCCAGCTG CTCCCCCCAC AGCCACCTTC CCTGCCCGGC AGTCTGGCCT GGGAAGCCAG
    GAGGTTGTGA ACAAGGTTGC TACCAGAAAA ATCCCCATGG AAAGCCAGAG AGATTCCACA
    TTCCCCAAAT TTGAGAGCAA GCCCCAAAGC CAGGAGGTCA GTGAAGATGA AACAGTCAAG
    TTCAGATGTG AGGTTTCAGG GACCCCAAAG CCTGAAGTGA CCTGGTTCCT GGAAGGTGCC
    CCCGTGAAGA GACGAGAAGG CATGGTTGAG GTTTATGAAG AAGGTGGATG CCACTACCTC
    TGCTTGCTGA GAGTGCGGGC CAAGGACAGT GGGAGCTACA GTTGCACGGC CTCCAATGTC
    CGAGGCCAGG TGTCCTGTGG TTGGACCCTC CTGGTGAAAC GGCTGGCCGT GATGGAGGTG
    GCTCCCTCGT TCTCCAGCGT CCTGAAGGAC TGCGCTGTCA TTGAGGGCCA GGACTTTGTG
    CTGCAGTGCT CAGTGCAGGG GACCCCGGTG CCCCAAATCA CTTGGCTGCT TAATGAGCAG
    CCCATCCAGT ACGCTCACTC CTCCTGCGAG GCTGGCGTGG CCGAGCTCCA CATCCAGGAT
    GCCCTGCCGG AAGATGATGG CATTTACACC TGTCTGGCCA AGAACACCTT GGGCCAGGTG
    TCCTGCAGCG CCCGGGTCAC CGTCCATGAA AAGAAGAGCG ACAGGAAGAG TGGGTATCTT
    CTGCGCACAG CTCCCGGCAA GCCCGTCGCA CCCCTCTTCC TGCAGGGCCT CTCTGACCTC
    AAAGTCATGG ATGGAAGCCA GGTCACCATG ACAGTCCAGG TGTCAGGGAA TCCGCCCCCC
    GAGGTCATCT GGCTTCACAA CGGGAATGAG ATCCAGGAGT CAGAGGACTT CCACTTTGAA
    CAGAGAGGCA CTCAGCACAG CCTTTGTATC CAGGAAGTGT TCCCAGAGGA CACAGGCACT
    TACACCTGCG AAGCCTGGAA CAGTGCTGGA GAGGTCCGCA CCCAGGCAGT ACTGATGGTG
    CAAGAGCCCC AGGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCCCGCTC ACTGACTGCC
    TCCCTGGGCC AGACTGTCCT CATCTCCTGT GCCATCGCTG GGGACCCCTT CCCCACTGTG
    CACTGGCTGC GAGATGGCAA AGCCCTCTCC AAAGACTCCG GCCACTTCGA GGTGCTACAG
    AACGAGGACG TGTTCACCCT GGTTCTAAAG AATGTGCAGC CCTGGCACGC CGGCCAGTAC
    GAGATCCAGC TCAAGAACCG GGTTGGCGAA TGCAGTTGCC AGGTGTCACT GATGCTACAG
    AGCAGCCCCA CCGGAGCCCC CCTGCGGGGG AGGGAGCCTG CCAGCTGCGA GGGCCTCTGC
    AGCCGGGGAG CCGGCGCTGA TGGTGGTGGC GGTGACTGCT ATGGGACCCT GAGGCCTGGC
    CGGCCAGCGA GAGGACAGGG TTGGCCAGAG GAGGAGGACG ACGGCGAGGA CGTGCGGGGG
    GTGCTGAAGA GGCGCGTGGA GACCCGGCAA CATACTGAGG AGGCCATCCG CCAGCAGCAG
    GTGGAACAGC TGGACTTCCG TGACCTCCTG GGGAAGAAGG TGAGTACCAA GACCGTGTCA
    GAAGAAGACC TGAAGGAGAT CCCAGCGGAG CAGATGGATT TCCGCGCCAA CCTGCAGCGG
    CAAGTGAAGC CAAAGACTGT GTCAGAGGAA GAGAGGAAGG TGCATAGCCC TCAGCAGGTC
    GACTTCCGCT CGGTCCTGGC CAAAAAGGGG ACTCCTAAGA CCCCGGTGCC TGAGAAGGCA
    CCTCTTCCCA AACCCGCCAC CCCAGATTTC CGCTCCGTGT TAGGTAGCAA GAAAAAATTA
    CCAGCCGAGA ATGGTAGCAA CAACGCTGAG GCCTTGAATG CCAAGGCAGC AGAAAGTCCC
    AAGGCTGTGA GCAATGCCCA GCCTTTAGGG TCCCTGAAAC CTTTGGGCAA TGCCAAGCCT
    GCTGAGACTC TAAAACCTGT GGGCAACATC AGGCCTGCTG AGACGCTGAA ACCCATAGGC
    AACGCCAAAC CTGCTGAGAC CCTGAAACCC ATGGGCAACG CCAAGCCTGC TGAGACGCTA
    AAACCCATAG GCAATGCCAA GCTTGCTGAG ACCCCGAAAC CCATGGGCAA TGCCAAGCCT
    GCTGAGACCC TGAAACCCCT GGGCAACGCC AAGCCTGCTG AGACGCTGAA ACCCATAAGC
    AACGCCAAGC CTGCTGAACC TCTGAAACCT GTGGGCAACG CCAAGCCTGC TGAGACCCCA
    AAACCCATAG GTAATGCCAA GCCTGCTGAG ATCCTAAAAC CAGCTGGGAA GGAAGAACTC
    AAGAAGGAGG TTAAGAATGA CGTGAACTGC AAGAGGGCAC AGGCAGGAGC CACAGATAAT
    GAAAAAAGAT CAGAGAACCA AGGGACAGCC CCAGCCTTCA AGGAGAAGCT GCAAGATGTC
    CATGTGACAG AGGGTGAGAA GCTGCTACTC CAGTGTCAGG TGTCGTCTGA CCCTCCAGCC
    ACCATCACCT GGATGCTCAA TGGAAAGACA CTCAAGACCA CCAAGTTCAT CATCCTCTCC
    CAAGAAGGCT CACTGTGCTC CGTGTCCATC GAGAAGGCCC TGCCCGAGGA CAGAGGCTTC
    TACAAGTGCA TAGCTAAGAA TGGCGCCGGC CAGGCTGAGT GCTCCTGCCA AGTCACCGTG
    GACGATGCTC CCACCAAGGA GAATGCCAAG GCCCCGGAGA TGAAAGCCCG GAGGCCCAAG
    AGCTCTCTAC CTCCCATGCT AGGAACAGAG AGTGATGCAA CTGTGAAAAA GAAACCTGCC
    CCCAAGACAC CTCCAAAAGC AGCCATGCCC CCTCAGATCA TCCAGTTTCC TGAGGACCAA
    AAGGTGCGCG CAGGAGAGTC TGTGGAGCTG TTTGGTAAAG TGGCAGGCAC CCAGCCCATC
    ACCTGTACGT GGATGAAGTT CCGAAAGCAG ATCCAGGAGA GCGAGCACAT CAAGGTGGAG
    ACCAGTGAGA ATGGCAGCAG GCTCACCATC GTGGCCGCAC GCCAGGAACA CTGCGGCTGC
    TACACTCTGC TGGTGGAGAA CAAGCTGGGC AGCAGGCAGG CCCAGGTCAA CCTCACTGTC
    GTGGACAAAC CAGACCCCCC AGCGGGCACA CCTTGCGCCT CCGACATCCG GAGCTCGTCG
    TTGACCCTGT CCTGGTATGG CTCCTCGTAT GATGGGGGCA GTGCCGTGCA ATCCTACAGC
    GTCGAGATCT GGGACTCGGT GGACAAGACG TGGAAGGAAC TAGCCACGTG CCGCAGCACC
    TCTTTTAACG TCCAGGACCT GCTGCCTGAC CGAGAGTATA AATTCCGCGT TCGCGCCATC
    AACGTGTATG GAACCAGTGA GCCAAGCCAG GAGTCTGAAC TCACAGCAGT GGGAGAGAAA
    CCGGAGGAGC CGAAGGACGA AGTGGAGGTG TCGGATGATG ATGAGAAGGA ACCTGAGGTC
    GACTACCGGA CAGTGACGGT CAACACTGAA CAAAAAGTGT CCGACTTCTA TGACATCGAA
    GAGAGACTAG GCTCTGGGAA ATTTGGACAG GTCTTCCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCA TATTCAGCCA AAGAGAAGGA GAATATCCGG
    CAGGAGATTA GCATCATGAA CTGCCTCCAC CACCCCAAGC TGGTCCAGTG TGTGGATGCC
    TTCGAAGAAA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCGGGGGG CGAGCTGTTC
    GAGCGCATCA TCGACGAGGA CTTCGAGCTG ACGGAGCGCG AGTGCATCCA GTACATGAGG
    CAGATCTCGG CGGGCGTGGA GTACATCCAC CGGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGCGT CAATAAGACC GGCACGAGGA TCAAGCTCAT CGACTTCGGC
    CTGGCCAGAA AGCTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGTAC CCCGGAATTT
    GTGGCCCCCG AAGTGATCAA CTATGAGCCC ATCGGCTATG CCACGGACAT GTGGAGCATC
    GGAGTCATCT GCTACATCCT GGTTAGTGGA CTCTCCCCCT TCATGGGTGA CAACGATAAT
    GAAACCCTAG CCAACGTTAC CTCAGCCACC TGGGACTTCG ATGATGAGGC GTTCGATGAG
    ATCTCGGATG ACGCCAAGGA TTTTATCAGC AATTTGCTGA AGAAAGATAT GAAAAACCGC
    CTGGACTGTA CCCAGTGCCT TCAGCATCCC TGGCTCATGA AAGACACCAA GAACATGGAA
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGATCACCG ACCAGCCCGC TCAACGCAGA AAAACTAGAA
    TCTGAAGAAG ATGTGTCCCA AGCTTTCCTT GAGGCTGTTG CTGAGGAAAA GCCCCATGTA
    AAACCCTATT TCTCTAAGAC CATTCGTGAT TTAGAAGTTG TGGAGGGAAG CGCTGCTAGA
    TTTGACTGCA AGATTGAAGG ATACCCAGAC CCTGAAGTTG TCTGGTTTAA AGATGACCAG
    TCAATCCGGG AGTCCCGCCA CTTCCAGATA GACTACGACG AGGATGGGAA CTGCTCTTTA
    ATCATTAGCG ATGTTTGTGG GGATGACGAT GCCAAGTACA CCTGCAAGGC TGTCAATAGT
    CTTGGAGAAG CCACTTGCAC AGCAGAGCTC ATTGTGGAGA CCATGGAGGA AGGGGAAGGG
    GAAGGGGAAG AGGAAGAAGA GGAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44724
    Clone ID Related Accession (Same CDS sequence) XM_014110234.2 , XM_014110234.1
    Accession Version XM_014110234.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6027bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGGAGAAC TGCCCGGGGA GGAGGCCACG ACCATGGGGG ATGTGAAGCT GGCCGCCTTG 
    ACACAGGCTT CTAAAACCTC CGTCAGTGTG GATCACTCGA GGGCCGGGTC TGCACCCCCG
    ACAGAGGCCC CTGCTTTCAT TTTGCCCCCT CGGAACCTCT GCATCAGAGA AGGAGCCACC
    GCCAAGTTTG AAGGGAGGGT CCGGGGTTAC CCAGAGCCCC AGGTGACATG GCACCGAAAT
    GGGCAACCCA TCACCAGTGG GGGCCGCTTC CTGCTGGATT GTGGTATCCG GGGGACTTTC
    AGCCTCGTGA TTCATGCTGT CCGTGAGGAG GACCAGGGAA AGTATACCTG CGAAGCCACC
    AATGATGGTG GTGCCCGCCA GGTGACAGTG GAGTTGACTG TAGAAGGGGG CTTCATGAAG
    AAGCATGGTC AGCCCGTTGT TTCCAAAACC TTGGGGGACA GATTTGCAGC CCCCGCAGTG
    GAGACCCGTC CTAGCATCTG GGGAGAGTGC CCACCAAAGT TTGCTACCAA GTTGGGCCGA
    GCCGTAGTCA AAGAAGGACA GACGGGACGG TTCTCCTGCA AGATCACTGG ACGGCCCCAA
    CCACAGGTCA CCTGGCTCAA GGGGGATGTT GCCCTGCAGC CAAGTGCCCG TGTGTCTATG
    TCTGAGAAGA ACGGGGTACA AGTTCTGGAA ATCCATGAGG TCAGCCGCGA TGATGTGGGA
    GTGTACACAT GCATGGTGGT GAATGGGTCG GGGAAGGCCT CCATGTCGGC TGAGCTTTCT
    ATCCAAGGTT TGGACAATAC CAGTAGGTCA TTAACAAAGG TGGCCAATTC AGATATCAGG
    AAGGAGGTGA CCAATGGAAC CACGCAGGGG CCGAAACTAG ACCACCTGGA GTCTGCAGCG
    GAAAGGTGGC CCTGCTCCAG CACCCAGATC GGTGGCTCCC CCACCTGGGC CACAAGTAGC
    CAGCCTCAGC CCCTGAGGGA GTCCAAGCTG GAGCCGCCTG GGGACCCACC CAGGAAAGCC
    CTGAGGAGCC CCGTCCTACA GAAGACTTCC AGCACCATTA CCCTGCAGGC GGCAAAAGTC
    CAGCCAGAGC CAAGAGCGCC GGTCTCAAGT GCCCTCTCGT CTCCCGGAGA AGAGAGGGAG
    AGGCCAGCTG CTCCCCCCAC AGCCACCTTC CCTGCCCGGC AGTCTGGCCT GGGAAGCCAG
    GAGGTTGTGA ACAAGGTTGC TACCAGAAAA ATCCCCATGG AAAGCCAGAG AGATTCCACA
    TTCCCCAAAT TTGAGAGCAA GCCCCAAAGC CAGGAGGTCA GTGAAGATGA AACAGTCAAG
    TTCAGATGTG AGGTTTCAGG GACCCCAAAG CCTGAAGTGA CCTGGTTCCT GGAAGGTGCC
    CCCGTGAAGA GACGAGAAGG CATGGTTGAG GTTTATGAAG AAGGTGGATG CCACTACCTC
    TGCTTGCTGA GAGTGCGGGC CAAGGACAGT GGGAGCTACA GTTGCACGGC CTCCAATGTC
    CGAGGCCAGG TGTCCTGTGG TTGGACCCTC CTGGTGAAAC GGCTGGCCGT GATGGAGGTG
    GCTCCCTCGT TCTCCAGCGT CCTGAAGGAC TGCGCTGTCA TTGAGGGCCA GGACTTTGTG
    CTGCAGTGCT CAGTGCAGGG GACCCCGGTG CCCCAAATCA CTTGGCTGCT TAATGAGCAG
    CCCATCCAGT ACGCTCACTC CTCCTGCGAG GCTGGCGTGG CCGAGCTCCA CATCCAGGAT
    GCCCTGCCGG AAGATGATGG CATTTACACC TGTCTGGCCA AGAACACCTT GGGCCAGGTG
    TCCTGCAGCG CCCGGGTCAC CGTCCATGAA AAGAAGAGCG ACAGGAAGAG TGGGTATCTT
    CTGCGCACAG CTCCCGGCAA GCCCGTCGCA CCCCTCTTCC TGCAGGGCCT CTCTGACCTC
    AAAGTCATGG ATGGAAGCCA GGTCACCATG ACAGTCCAGG TGTCAGGGAA TCCGCCCCCC
    GAGGTCATCT GGCTTCACAA CGGGAATGAG ATCCAGGAGT CAGAGGACTT CCACTTTGAA
    CAGAGAGGCA CTCAGCACAG CCTTTGTATC CAGGAAGTGT TCCCAGAGGA CACAGGCACT
    TACACCTGCG AAGCCTGGAA CAGTGCTGGA GAGGTCCGCA CCCAGGCAGT ACTGATGGTG
    CAAGAGCCCC AGGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCCCGCTC ACTGACTGCC
    TCCCTGGGCC AGACTGTCCT CATCTCCTGT GCCATCGCTG GGGACCCCTT CCCCACTGTG
    CACTGGCTGC GAGATGGCAA AGCCCTCTCC AAAGACTCCG GCCACTTCGA GGTGCTACAG
    AACGAGGACG TGTTCACCCT GGTTCTAAAG AATGTGCAGC CCTGGCACGC CGGCCAGTAC
    GAGATCCAGC TCAAGAACCG GGTTGGCGAA TGCAGTTGCC AGGTGTCACT GATGCTACAG
    AGCAGCCCCA CCGGAGCCCC CCTGCGGGGG AGGGAGCCTG CCAGCTGCGA GGGCCTCTGC
    AGCCGGGGAG CCGGCGCTGA TGGTGGTGGC GGTGACTGCT ATGGGACCCT GAGGCCTGGC
    CGGCCAGCGA GAGGACAGGG TTGGCCAGAG GAGGAGGACG ACGGCGAGGA CGTGCGGGGG
    GTGCTGAAGA GGCGCGTGGA GACCCGGCAA CATACTGAGG AGGCCATCCG CCAGCAGCAG
    GTGGAACAGC TGGACTTCCG TGACCTCCTG GGGAAGAAGG TGAGTACCAA GACCGTGTCA
    GAAGAAGACC TGAAGGAGAT CCCAGCGGAG CAGATGGATT TCCGCGCCAA CCTGCAGCGG
    CAAGTGAAGC CAAAGACTGT GTCAGAGGAA GAGAGGAAGG TGCATAGCCC TCAGCAGGTC
    GACTTCCGCT CGGTCCTGGC CAAAAAGGGG ACTCCTAAGA CCCCGGTGCC TGAGAAGGCA
    CCTCTTCCCA AACCCGCCAC CCCAGATTTC CGCTCCGTGT TAGGTAGCAA GAAAAAATTA
    CCAGCCGAGA ATGGTAGCAA CAACGCTGAG GCCTTGAATG CCAAGGCAGC AGAAAGTCCC
    AAGGCTGTGA GCAATGCCCA GCCTTTAGGG TCCCTGAAAC CTTTGGGCAA TGCCAAGCCT
    GCTGAGACTC TAAAACCTGT GGGCAACATC AGGCCTGCTG AGACGCTGAA ACCCATAGGC
    AACGCCAAAC CTGCTGAGAC CCTGAAACCC ATGGGCAACG CCAAGCCTGC TGAGACGCTA
    AAACCCATAG GCAATGCCAA GCTTGCTGAG ACCCCGAAAC CCATGGGCAA TGCCAAGCCT
    GCTGAGACCC TGAAACCCCT GGGCAACGCC AAGCCTGCTG AGACGCTGAA ACCCATAAGC
    AACGCCAAGC CTGCTGAACC TCTGAAACCT GTGGGCAACG CCAAGCCTGC TGAGACCCCA
    AAACCCATAG GTAATGCCAA GCCTGCTGAG ATCCTAAAAC CAGCTGGGAA GGAAGAACTC
    AAGAAGGAGG TTAAGAATGA CGTGAACTGC AAGAGGGCAC AGGCAGGAGC CACAGATAAT
    GAAAAAAGAT CAGAGAACCA AGGGACAGCC CCAGCCTTCA AGGAGAAGCT GCAAGATGTC
    CATGTGACAG AGGGTGAGAA GCTGCTACTC CAGTGTCAGG TGTCGTCTGA CCCTCCAGCC
    ACCATCACCT GGATGCTCAA TGGAAAGACA CTCAAGACCA CCAAGTTCAT CATCCTCTCC
    CAAGAAGGCT CACTGTGCTC CGTGTCCATC GAGAAGGCCC TGCCCGAGGA CAGAGGCTTC
    TACAAGTGCA TAGCTAAGAA TGGCGCCGGC CAGGCTGAGT GCTCCTGCCA AGTCACCGTG
    GACGATGCTC CCACCAAGGA GAATGCCAAG GCCCCGGAGA TGAAAGCCCG GAGGCCCAAG
    AGCTCTCTAC CTCCCATGCT AGGAACAGAG AGTGATGCAA CTGTGAAAAA GAAACCTGCC
    CCCAAGACAC CTCCAAAAGC AGCCATGCCC CCTCAGATCA TCCAGTTTCC TGAGGACCAA
    AAGGTGCGCG CAGGAGAGTC TGTGGAGCTG TTTGGTAAAG TGGCAGGCAC CCAGCCCATC
    ACCTGTACGT GGATGAAGTT CCGAAAGCAG ATCCAGGAGA GCGAGCACAT CAAGGTGGAG
    ACCAGTGAGA ATGGCAGCAG GCTCACCATC GTGGCCGCAC GCCAGGAACA CTGCGGCTGC
    TACACTCTGC TGGTGGAGAA CAAGCTGGGC AGCAGGCAGG CCCAGGTCAA CCTCACTGTC
    GTGGACAAAC CAGACCCCCC AGCGGGCACA CCTTGCGCCT CCGACATCCG GAGCTCGTCG
    TTGACCCTGT CCTGGTATGG CTCCTCGTAT GATGGGGGCA GTGCCGTGCA ATCCTACAGC
    GTCGAGATCT GGGACTCGGT GGACAAGACG TGGAAGGAAC TAGCCACGTG CCGCAGCACC
    TCTTTTAACG TCCAGGACCT GCTGCCTGAC CGAGAGTATA AATTCCGCGT TCGCGCCATC
    AACGTGTATG GAACCAGTGA GCCAAGCCAG GAGTCTGAAC TCACAGCAGT GGGAGAGAAA
    CCGGAGGAGC CGAAGGACGA AGTGGAGGTG TCGGATGATG ATGAGAAGGA ACCTGAGGTC
    GACTACCGGA CAGTGACGGT CAACACTGAA CAAAAAGTGT CCGACTTCTA TGACATCGAA
    GAGAGACTAG GCTCTGGGAA ATTTGGACAG GTCTTCCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCA TATTCAGCCA AAGAGAAGGA GAATATCCGG
    CAGGAGATTA GCATCATGAA CTGCCTCCAC CACCCCAAGC TGGTCCAGTG TGTGGATGCC
    TTCGAAGAAA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCGGGGGG CGAGCTGTTC
    GAGCGCATCA TCGACGAGGA CTTCGAGCTG ACGGAGCGCG AGTGCATCCA GTACATGAGG
    CAGATCTCGG CGGGCGTGGA GTACATCCAC CGGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGCGT CAATAAGACC GGCACGAGGA TCAAGCTCAT CGACTTCGGC
    CTGGCCAGAA AGCTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGTAC CCCGGAATTT
    GTGGCCCCCG AAGTGATCAA CTATGAGCCC ATCGGCTATG CCACGGACAT GTGGAGCATC
    GGAGTCATCT GCTACATCCT GGTTAGTGGA CTCTCCCCCT TCATGGGTGA CAACGATAAT
    GAAACCCTAG CCAACGTTAC CTCAGCCACC TGGGACTTCG ATGATGAGGC GTTCGATGAG
    ATCTCGGATG ACGCCAAGGA TTTTATCAGC AATTTGCTGA AGAAAGATAT GAAAAACCGC
    CTGGACTGTA CCCAGTGCCT TCAGCATCCC TGGCTCATGA AAGACACCAA GAACATGGAA
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGATCACCG ACCAGCCCGC TCAACGCAGA AAAACTAGAA
    TCTGAAGATG TGTCCCAAGC TTTCCTTGAG GCTGTTGCTG AGGAAAAGCC CCATGTAAAA
    CCCTATTTCT CTAAGACCAT TCGTGATTTA GAAGTTGTGG AGGGAAGCGC TGCTAGATTT
    GACTGCAAGA TTGAAGGATA CCCAGACCCT GAAGTTGTCT GGTTTAAAGA TGACCAGTCA
    ATCCGGGAGT CCCGCCACTT CCAGATAGAC TACGACGAGG ATGGGAACTG CTCTTTAATC
    ATTAGCGATG TTTGTGGGGA TGACGATGCC AAGTACACCT GCAAGGCTGT CAATAGTCTT
    GGAGAAGCCA CTTGCACAGC AGAGCTCATT GTGGAGACCA TGGAGGAAGG GGAAGGGGAA
    GGGGAAGAGG AAGAAGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965709.1
    CDS93..6119
    Translation

    Target ORF information:

    RefSeq Version XM_014110234.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110234.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGGAGAAC TGCCCGGGGA GGAGGCCACG ACCATGGGGG ATGTGAAGCT GGCCGCCTTG 
    ACACAGGCTT CTAAAACCTC CGTCAGTGTG GATCACTCGA GGGCCGGGTC TGCACCCCCG
    ACAGAGGCCC CTGCTTTCAT TTTGCCCCCT CGGAACCTCT GCATCAGAGA AGGAGCCACC
    GCCAAGTTTG AAGGGAGGGT CCGGGGTTAC CCAGAGCCCC AGGTGACATG GCACCGAAAT
    GGGCAACCCA TCACCAGTGG GGGCCGCTTC CTGCTGGATT GTGGTATCCG GGGGACTTTC
    AGCCTCGTGA TTCATGCTGT CCGTGAGGAG GACCAGGGAA AGTATACCTG CGAAGCCACC
    AATGATGGTG GTGCCCGCCA GGTGACAGTG GAGTTGACTG TAGAAGGGGG CTTCATGAAG
    AAGCATGGTC AGCCCGTTGT TTCCAAAACC TTGGGGGACA GATTTGCAGC CCCCGCAGTG
    GAGACCCGTC CTAGCATCTG GGGAGAGTGC CCACCAAAGT TTGCTACCAA GTTGGGCCGA
    GCCGTAGTCA AAGAAGGACA GACGGGACGG TTCTCCTGCA AGATCACTGG ACGGCCCCAA
    CCACAGGTCA CCTGGCTCAA GGGGGATGTT GCCCTGCAGC CAAGTGCCCG TGTGTCTATG
    TCTGAGAAGA ACGGGGTACA AGTTCTGGAA ATCCATGAGG TCAGCCGCGA TGATGTGGGA
    GTGTACACAT GCATGGTGGT GAATGGGTCG GGGAAGGCCT CCATGTCGGC TGAGCTTTCT
    ATCCAAGGTT TGGACAATAC CAGTAGGTCA TTAACAAAGG TGGCCAATTC AGATATCAGG
    AAGGAGGTGA CCAATGGAAC CACGCAGGGG CCGAAACTAG ACCACCTGGA GTCTGCAGCG
    GAAAGGTGGC CCTGCTCCAG CACCCAGATC GGTGGCTCCC CCACCTGGGC CACAAGTAGC
    CAGCCTCAGC CCCTGAGGGA GTCCAAGCTG GAGCCGCCTG GGGACCCACC CAGGAAAGCC
    CTGAGGAGCC CCGTCCTACA GAAGACTTCC AGCACCATTA CCCTGCAGGC GGCAAAAGTC
    CAGCCAGAGC CAAGAGCGCC GGTCTCAAGT GCCCTCTCGT CTCCCGGAGA AGAGAGGGAG
    AGGCCAGCTG CTCCCCCCAC AGCCACCTTC CCTGCCCGGC AGTCTGGCCT GGGAAGCCAG
    GAGGTTGTGA ACAAGGTTGC TACCAGAAAA ATCCCCATGG AAAGCCAGAG AGATTCCACA
    TTCCCCAAAT TTGAGAGCAA GCCCCAAAGC CAGGAGGTCA GTGAAGATGA AACAGTCAAG
    TTCAGATGTG AGGTTTCAGG GACCCCAAAG CCTGAAGTGA CCTGGTTCCT GGAAGGTGCC
    CCCGTGAAGA GACGAGAAGG CATGGTTGAG GTTTATGAAG AAGGTGGATG CCACTACCTC
    TGCTTGCTGA GAGTGCGGGC CAAGGACAGT GGGAGCTACA GTTGCACGGC CTCCAATGTC
    CGAGGCCAGG TGTCCTGTGG TTGGACCCTC CTGGTGAAAC GGCTGGCCGT GATGGAGGTG
    GCTCCCTCGT TCTCCAGCGT CCTGAAGGAC TGCGCTGTCA TTGAGGGCCA GGACTTTGTG
    CTGCAGTGCT CAGTGCAGGG GACCCCGGTG CCCCAAATCA CTTGGCTGCT TAATGAGCAG
    CCCATCCAGT ACGCTCACTC CTCCTGCGAG GCTGGCGTGG CCGAGCTCCA CATCCAGGAT
    GCCCTGCCGG AAGATGATGG CATTTACACC TGTCTGGCCA AGAACACCTT GGGCCAGGTG
    TCCTGCAGCG CCCGGGTCAC CGTCCATGAA AAGAAGAGCG ACAGGAAGAG TGGGTATCTT
    CTGCGCACAG CTCCCGGCAA GCCCGTCGCA CCCCTCTTCC TGCAGGGCCT CTCTGACCTC
    AAAGTCATGG ATGGAAGCCA GGTCACCATG ACAGTCCAGG TGTCAGGGAA TCCGCCCCCC
    GAGGTCATCT GGCTTCACAA CGGGAATGAG ATCCAGGAGT CAGAGGACTT CCACTTTGAA
    CAGAGAGGCA CTCAGCACAG CCTTTGTATC CAGGAAGTGT TCCCAGAGGA CACAGGCACT
    TACACCTGCG AAGCCTGGAA CAGTGCTGGA GAGGTCCGCA CCCAGGCAGT ACTGATGGTG
    CAAGAGCCCC AGGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCCCGCTC ACTGACTGCC
    TCCCTGGGCC AGACTGTCCT CATCTCCTGT GCCATCGCTG GGGACCCCTT CCCCACTGTG
    CACTGGCTGC GAGATGGCAA AGCCCTCTCC AAAGACTCCG GCCACTTCGA GGTGCTACAG
    AACGAGGACG TGTTCACCCT GGTTCTAAAG AATGTGCAGC CCTGGCACGC CGGCCAGTAC
    GAGATCCAGC TCAAGAACCG GGTTGGCGAA TGCAGTTGCC AGGTGTCACT GATGCTACAG
    AGCAGCCCCA CCGGAGCCCC CCTGCGGGGG AGGGAGCCTG CCAGCTGCGA GGGCCTCTGC
    AGCCGGGGAG CCGGCGCTGA TGGTGGTGGC GGTGACTGCT ATGGGACCCT GAGGCCTGGC
    CGGCCAGCGA GAGGACAGGG TTGGCCAGAG GAGGAGGACG ACGGCGAGGA CGTGCGGGGG
    GTGCTGAAGA GGCGCGTGGA GACCCGGCAA CATACTGAGG AGGCCATCCG CCAGCAGCAG
    GTGGAACAGC TGGACTTCCG TGACCTCCTG GGGAAGAAGG TGAGTACCAA GACCGTGTCA
    GAAGAAGACC TGAAGGAGAT CCCAGCGGAG CAGATGGATT TCCGCGCCAA CCTGCAGCGG
    CAAGTGAAGC CAAAGACTGT GTCAGAGGAA GAGAGGAAGG TGCATAGCCC TCAGCAGGTC
    GACTTCCGCT CGGTCCTGGC CAAAAAGGGG ACTCCTAAGA CCCCGGTGCC TGAGAAGGCA
    CCTCTTCCCA AACCCGCCAC CCCAGATTTC CGCTCCGTGT TAGGTAGCAA GAAAAAATTA
    CCAGCCGAGA ATGGTAGCAA CAACGCTGAG GCCTTGAATG CCAAGGCAGC AGAAAGTCCC
    AAGGCTGTGA GCAATGCCCA GCCTTTAGGG TCCCTGAAAC CTTTGGGCAA TGCCAAGCCT
    GCTGAGACTC TAAAACCTGT GGGCAACATC AGGCCTGCTG AGACGCTGAA ACCCATAGGC
    AACGCCAAAC CTGCTGAGAC CCTGAAACCC ATGGGCAACG CCAAGCCTGC TGAGACGCTA
    AAACCCATAG GCAATGCCAA GCTTGCTGAG ACCCCGAAAC CCATGGGCAA TGCCAAGCCT
    GCTGAGACCC TGAAACCCCT GGGCAACGCC AAGCCTGCTG AGACGCTGAA ACCCATAAGC
    AACGCCAAGC CTGCTGAACC TCTGAAACCT GTGGGCAACG CCAAGCCTGC TGAGACCCCA
    AAACCCATAG GTAATGCCAA GCCTGCTGAG ATCCTAAAAC CAGCTGGGAA GGAAGAACTC
    AAGAAGGAGG TTAAGAATGA CGTGAACTGC AAGAGGGCAC AGGCAGGAGC CACAGATAAT
    GAAAAAAGAT CAGAGAACCA AGGGACAGCC CCAGCCTTCA AGGAGAAGCT GCAAGATGTC
    CATGTGACAG AGGGTGAGAA GCTGCTACTC CAGTGTCAGG TGTCGTCTGA CCCTCCAGCC
    ACCATCACCT GGATGCTCAA TGGAAAGACA CTCAAGACCA CCAAGTTCAT CATCCTCTCC
    CAAGAAGGCT CACTGTGCTC CGTGTCCATC GAGAAGGCCC TGCCCGAGGA CAGAGGCTTC
    TACAAGTGCA TAGCTAAGAA TGGCGCCGGC CAGGCTGAGT GCTCCTGCCA AGTCACCGTG
    GACGATGCTC CCACCAAGGA GAATGCCAAG GCCCCGGAGA TGAAAGCCCG GAGGCCCAAG
    AGCTCTCTAC CTCCCATGCT AGGAACAGAG AGTGATGCAA CTGTGAAAAA GAAACCTGCC
    CCCAAGACAC CTCCAAAAGC AGCCATGCCC CCTCAGATCA TCCAGTTTCC TGAGGACCAA
    AAGGTGCGCG CAGGAGAGTC TGTGGAGCTG TTTGGTAAAG TGGCAGGCAC CCAGCCCATC
    ACCTGTACGT GGATGAAGTT CCGAAAGCAG ATCCAGGAGA GCGAGCACAT CAAGGTGGAG
    ACCAGTGAGA ATGGCAGCAG GCTCACCATC GTGGCCGCAC GCCAGGAACA CTGCGGCTGC
    TACACTCTGC TGGTGGAGAA CAAGCTGGGC AGCAGGCAGG CCCAGGTCAA CCTCACTGTC
    GTGGACAAAC CAGACCCCCC AGCGGGCACA CCTTGCGCCT CCGACATCCG GAGCTCGTCG
    TTGACCCTGT CCTGGTATGG CTCCTCGTAT GATGGGGGCA GTGCCGTGCA ATCCTACAGC
    GTCGAGATCT GGGACTCGGT GGACAAGACG TGGAAGGAAC TAGCCACGTG CCGCAGCACC
    TCTTTTAACG TCCAGGACCT GCTGCCTGAC CGAGAGTATA AATTCCGCGT TCGCGCCATC
    AACGTGTATG GAACCAGTGA GCCAAGCCAG GAGTCTGAAC TCACAGCAGT GGGAGAGAAA
    CCGGAGGAGC CGAAGGACGA AGTGGAGGTG TCGGATGATG ATGAGAAGGA ACCTGAGGTC
    GACTACCGGA CAGTGACGGT CAACACTGAA CAAAAAGTGT CCGACTTCTA TGACATCGAA
    GAGAGACTAG GCTCTGGGAA ATTTGGACAG GTCTTCCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCA TATTCAGCCA AAGAGAAGGA GAATATCCGG
    CAGGAGATTA GCATCATGAA CTGCCTCCAC CACCCCAAGC TGGTCCAGTG TGTGGATGCC
    TTCGAAGAAA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCGGGGGG CGAGCTGTTC
    GAGCGCATCA TCGACGAGGA CTTCGAGCTG ACGGAGCGCG AGTGCATCCA GTACATGAGG
    CAGATCTCGG CGGGCGTGGA GTACATCCAC CGGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGCGT CAATAAGACC GGCACGAGGA TCAAGCTCAT CGACTTCGGC
    CTGGCCAGAA AGCTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGTAC CCCGGAATTT
    GTGGCCCCCG AAGTGATCAA CTATGAGCCC ATCGGCTATG CCACGGACAT GTGGAGCATC
    GGAGTCATCT GCTACATCCT GGTTAGTGGA CTCTCCCCCT TCATGGGTGA CAACGATAAT
    GAAACCCTAG CCAACGTTAC CTCAGCCACC TGGGACTTCG ATGATGAGGC GTTCGATGAG
    ATCTCGGATG ACGCCAAGGA TTTTATCAGC AATTTGCTGA AGAAAGATAT GAAAAACCGC
    CTGGACTGTA CCCAGTGCCT TCAGCATCCC TGGCTCATGA AAGACACCAA GAACATGGAA
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGATCACCG ACCAGCCCGC TCAACGCAGA AAAACTAGAA
    TCTGAAGATG TGTCCCAAGC TTTCCTTGAG GCTGTTGCTG AGGAAAAGCC CCATGTAAAA
    CCCTATTTCT CTAAGACCAT TCGTGATTTA GAAGTTGTGG AGGGAAGCGC TGCTAGATTT
    GACTGCAAGA TTGAAGGATA CCCAGACCCT GAAGTTGTCT GGTTTAAAGA TGACCAGTCA
    ATCCGGGAGT CCCGCCACTT CCAGATAGAC TACGACGAGG ATGGGAACTG CTCTTTAATC
    ATTAGCGATG TTTGTGGGGA TGACGATGCC AAGTACACCT GCAAGGCTGT CAATAGTCTT
    GGAGAAGCCA CTTGCACAGC AGAGCTCATT GTGGAGACCA TGGAGGAAGG GGAAGGGGAA
    GGGGAAGAGG AAGAAGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44724
    Clone ID Related Accession (Same CDS sequence) XM_014110234.2 , XM_014110234.1
    Accession Version XM_014110234.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6027bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726118.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGGAGAAC TGCCCGGGGA GGAGGCCACG ACCATGGGGG ATGTGAAGCT GGCCGCCTTG 
    ACACAGGCTT CTAAAACCTC CGTCAGTGTG GATCACTCGA GGGCCGGGTC TGCACCCCCG
    ACAGAGGCCC CTGCTTTCAT TTTGCCCCCT CGGAACCTCT GCATCAGAGA AGGAGCCACC
    GCCAAGTTTG AAGGGAGGGT CCGGGGTTAC CCAGAGCCCC AGGTGACATG GCACCGAAAT
    GGGCAACCCA TCACCAGTGG GGGCCGCTTC CTGCTGGATT GTGGTATCCG GGGGACTTTC
    AGCCTCGTGA TTCATGCTGT CCGTGAGGAG GACCAGGGAA AGTATACCTG CGAAGCCACC
    AATGATGGTG GTGCCCGCCA GGTGACAGTG GAGTTGACTG TAGAAGGGGG CTTCATGAAG
    AAGCATGGTC AGCCCGTTGT TTCCAAAACC TTGGGGGACA GATTTGCAGC CCCCGCAGTG
    GAGACCCGTC CTAGCATCTG GGGAGAGTGC CCACCAAAGT TTGCTACCAA GTTGGGCCGA
    GCCGTAGTCA AAGAAGGACA GACGGGACGG TTCTCCTGCA AGATCACTGG ACGGCCCCAA
    CCACAGGTCA CCTGGCTCAA GGGGGATGTT GCCCTGCAGC CAAGTGCCCG TGTGTCTATG
    TCTGAGAAGA ACGGGGTACA AGTTCTGGAA ATCCATGAGG TCAGCCGCGA TGATGTGGGA
    GTGTACACAT GCATGGTGGT GAATGGGTCG GGGAAGGCCT CCATGTCGGC TGAGCTTTCT
    ATCCAAGGTT TGGACAATAC CAGTAGGTCA TTAACAAAGG TGGCCAATTC AGATATCAGG
    AAGGAGGTGA CCAATGGAAC CACGCAGGGG CCGAAACTAG ACCACCTGGA GTCTGCAGCG
    GAAAGGTGGC CCTGCTCCAG CACCCAGATC GGTGGCTCCC CCACCTGGGC CACAAGTAGC
    CAGCCTCAGC CCCTGAGGGA GTCCAAGCTG GAGCCGCCTG GGGACCCACC CAGGAAAGCC
    CTGAGGAGCC CCGTCCTACA GAAGACTTCC AGCACCATTA CCCTGCAGGC GGCAAAAGTC
    CAGCCAGAGC CAAGAGCGCC GGTCTCAAGT GCCCTCTCGT CTCCCGGAGA AGAGAGGGAG
    AGGCCAGCTG CTCCCCCCAC AGCCACCTTC CCTGCCCGGC AGTCTGGCCT GGGAAGCCAG
    GAGGTTGTGA ACAAGGTTGC TACCAGAAAA ATCCCCATGG AAAGCCAGAG AGATTCCACA
    TTCCCCAAAT TTGAGAGCAA GCCCCAAAGC CAGGAGGTCA GTGAAGATGA AACAGTCAAG
    TTCAGATGTG AGGTTTCAGG GACCCCAAAG CCTGAAGTGA CCTGGTTCCT GGAAGGTGCC
    CCCGTGAAGA GACGAGAAGG CATGGTTGAG GTTTATGAAG AAGGTGGATG CCACTACCTC
    TGCTTGCTGA GAGTGCGGGC CAAGGACAGT GGGAGCTACA GTTGCACGGC CTCCAATGTC
    CGAGGCCAGG TGTCCTGTGG TTGGACCCTC CTGGTGAAAC GGCTGGCCGT GATGGAGGTG
    GCTCCCTCGT TCTCCAGCGT CCTGAAGGAC TGCGCTGTCA TTGAGGGCCA GGACTTTGTG
    CTGCAGTGCT CAGTGCAGGG GACCCCGGTG CCCCAAATCA CTTGGCTGCT TAATGAGCAG
    CCCATCCAGT ACGCTCACTC CTCCTGCGAG GCTGGCGTGG CCGAGCTCCA CATCCAGGAT
    GCCCTGCCGG AAGATGATGG CATTTACACC TGTCTGGCCA AGAACACCTT GGGCCAGGTG
    TCCTGCAGCG CCCGGGTCAC CGTCCATGAA AAGAAGAGCG ACAGGAAGAG TGGGTATCTT
    CTGCGCACAG CTCCCGGCAA GCCCGTCGCA CCCCTCTTCC TGCAGGGCCT CTCTGACCTC
    AAAGTCATGG ATGGAAGCCA GGTCACCATG ACAGTCCAGG TGTCAGGGAA TCCGCCCCCC
    GAGGTCATCT GGCTTCACAA CGGGAATGAG ATCCAGGAGT CAGAGGACTT CCACTTTGAA
    CAGAGAGGCA CTCAGCACAG CCTTTGTATC CAGGAAGTGT TCCCAGAGGA CACAGGCACT
    TACACCTGCG AAGCCTGGAA CAGTGCTGGA GAGGTCCGCA CCCAGGCAGT ACTGATGGTG
    CAAGAGCCCC AGGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCCCGCTC ACTGACTGCC
    TCCCTGGGCC AGACTGTCCT CATCTCCTGT GCCATCGCTG GGGACCCCTT CCCCACTGTG
    CACTGGCTGC GAGATGGCAA AGCCCTCTCC AAAGACTCCG GCCACTTCGA GGTGCTACAG
    AACGAGGACG TGTTCACCCT GGTTCTAAAG AATGTGCAGC CCTGGCACGC CGGCCAGTAC
    GAGATCCAGC TCAAGAACCG GGTTGGCGAA TGCAGTTGCC AGGTGTCACT GATGCTACAG
    AGCAGCCCCA CCGGAGCCCC CCTGCGGGGG AGGGAGCCTG CCAGCTGCGA GGGCCTCTGC
    AGCCGGGGAG CCGGCGCTGA TGGTGGTGGC GGTGACTGCT ATGGGACCCT GAGGCCTGGC
    CGGCCAGCGA GAGGACAGGG TTGGCCAGAG GAGGAGGACG ACGGCGAGGA CGTGCGGGGG
    GTGCTGAAGA GGCGCGTGGA GACCCGGCAA CATACTGAGG AGGCCATCCG CCAGCAGCAG
    GTGGAACAGC TGGACTTCCG TGACCTCCTG GGGAAGAAGG TGAGTACCAA GACCGTGTCA
    GAAGAAGACC TGAAGGAGAT CCCAGCGGAG CAGATGGATT TCCGCGCCAA CCTGCAGCGG
    CAAGTGAAGC CAAAGACTGT GTCAGAGGAA GAGAGGAAGG TGCATAGCCC TCAGCAGGTC
    GACTTCCGCT CGGTCCTGGC CAAAAAGGGG ACTCCTAAGA CCCCGGTGCC TGAGAAGGCA
    CCTCTTCCCA AACCCGCCAC CCCAGATTTC CGCTCCGTGT TAGGTAGCAA GAAAAAATTA
    CCAGCCGAGA ATGGTAGCAA CAACGCTGAG GCCTTGAATG CCAAGGCAGC AGAAAGTCCC
    AAGGCTGTGA GCAATGCCCA GCCTTTAGGG TCCCTGAAAC CTTTGGGCAA TGCCAAGCCT
    GCTGAGACTC TAAAACCTGT GGGCAACATC AGGCCTGCTG AGACGCTGAA ACCCATAGGC
    AACGCCAAAC CTGCTGAGAC CCTGAAACCC ATGGGCAACG CCAAGCCTGC TGAGACGCTA
    AAACCCATAG GCAATGCCAA GCTTGCTGAG ACCCCGAAAC CCATGGGCAA TGCCAAGCCT
    GCTGAGACCC TGAAACCCCT GGGCAACGCC AAGCCTGCTG AGACGCTGAA ACCCATAAGC
    AACGCCAAGC CTGCTGAACC TCTGAAACCT GTGGGCAACG CCAAGCCTGC TGAGACCCCA
    AAACCCATAG GTAATGCCAA GCCTGCTGAG ATCCTAAAAC CAGCTGGGAA GGAAGAACTC
    AAGAAGGAGG TTAAGAATGA CGTGAACTGC AAGAGGGCAC AGGCAGGAGC CACAGATAAT
    GAAAAAAGAT CAGAGAACCA AGGGACAGCC CCAGCCTTCA AGGAGAAGCT GCAAGATGTC
    CATGTGACAG AGGGTGAGAA GCTGCTACTC CAGTGTCAGG TGTCGTCTGA CCCTCCAGCC
    ACCATCACCT GGATGCTCAA TGGAAAGACA CTCAAGACCA CCAAGTTCAT CATCCTCTCC
    CAAGAAGGCT CACTGTGCTC CGTGTCCATC GAGAAGGCCC TGCCCGAGGA CAGAGGCTTC
    TACAAGTGCA TAGCTAAGAA TGGCGCCGGC CAGGCTGAGT GCTCCTGCCA AGTCACCGTG
    GACGATGCTC CCACCAAGGA GAATGCCAAG GCCCCGGAGA TGAAAGCCCG GAGGCCCAAG
    AGCTCTCTAC CTCCCATGCT AGGAACAGAG AGTGATGCAA CTGTGAAAAA GAAACCTGCC
    CCCAAGACAC CTCCAAAAGC AGCCATGCCC CCTCAGATCA TCCAGTTTCC TGAGGACCAA
    AAGGTGCGCG CAGGAGAGTC TGTGGAGCTG TTTGGTAAAG TGGCAGGCAC CCAGCCCATC
    ACCTGTACGT GGATGAAGTT CCGAAAGCAG ATCCAGGAGA GCGAGCACAT CAAGGTGGAG
    ACCAGTGAGA ATGGCAGCAG GCTCACCATC GTGGCCGCAC GCCAGGAACA CTGCGGCTGC
    TACACTCTGC TGGTGGAGAA CAAGCTGGGC AGCAGGCAGG CCCAGGTCAA CCTCACTGTC
    GTGGACAAAC CAGACCCCCC AGCGGGCACA CCTTGCGCCT CCGACATCCG GAGCTCGTCG
    TTGACCCTGT CCTGGTATGG CTCCTCGTAT GATGGGGGCA GTGCCGTGCA ATCCTACAGC
    GTCGAGATCT GGGACTCGGT GGACAAGACG TGGAAGGAAC TAGCCACGTG CCGCAGCACC
    TCTTTTAACG TCCAGGACCT GCTGCCTGAC CGAGAGTATA AATTCCGCGT TCGCGCCATC
    AACGTGTATG GAACCAGTGA GCCAAGCCAG GAGTCTGAAC TCACAGCAGT GGGAGAGAAA
    CCGGAGGAGC CGAAGGACGA AGTGGAGGTG TCGGATGATG ATGAGAAGGA ACCTGAGGTC
    GACTACCGGA CAGTGACGGT CAACACTGAA CAAAAAGTGT CCGACTTCTA TGACATCGAA
    GAGAGACTAG GCTCTGGGAA ATTTGGACAG GTCTTCCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCA TATTCAGCCA AAGAGAAGGA GAATATCCGG
    CAGGAGATTA GCATCATGAA CTGCCTCCAC CACCCCAAGC TGGTCCAGTG TGTGGATGCC
    TTCGAAGAAA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCGGGGGG CGAGCTGTTC
    GAGCGCATCA TCGACGAGGA CTTCGAGCTG ACGGAGCGCG AGTGCATCCA GTACATGAGG
    CAGATCTCGG CGGGCGTGGA GTACATCCAC CGGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGCGT CAATAAGACC GGCACGAGGA TCAAGCTCAT CGACTTCGGC
    CTGGCCAGAA AGCTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGTAC CCCGGAATTT
    GTGGCCCCCG AAGTGATCAA CTATGAGCCC ATCGGCTATG CCACGGACAT GTGGAGCATC
    GGAGTCATCT GCTACATCCT GGTTAGTGGA CTCTCCCCCT TCATGGGTGA CAACGATAAT
    GAAACCCTAG CCAACGTTAC CTCAGCCACC TGGGACTTCG ATGATGAGGC GTTCGATGAG
    ATCTCGGATG ACGCCAAGGA TTTTATCAGC AATTTGCTGA AGAAAGATAT GAAAAACCGC
    CTGGACTGTA CCCAGTGCCT TCAGCATCCC TGGCTCATGA AAGACACCAA GAACATGGAA
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGATCACCG ACCAGCCCGC TCAACGCAGA AAAACTAGAA
    TCTGAAGATG TGTCCCAAGC TTTCCTTGAG GCTGTTGCTG AGGAAAAGCC CCATGTAAAA
    CCCTATTTCT CTAAGACCAT TCGTGATTTA GAAGTTGTGG AGGGAAGCGC TGCTAGATTT
    GACTGCAAGA TTGAAGGATA CCCAGACCCT GAAGTTGTCT GGTTTAAAGA TGACCAGTCA
    ATCCGGGAGT CCCGCCACTT CCAGATAGAC TACGACGAGG ATGGGAACTG CTCTTTAATC
    ATTAGCGATG TTTGTGGGGA TGACGATGCC AAGTACACCT GCAAGGCTGT CAATAGTCTT
    GGAGAAGCCA CTTGCACAGC AGAGCTCATT GTGGAGACCA TGGAGGAAGG GGAAGGGGAA
    GGGGAAGAGG AAGAAGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965709.1
    CDS266..6292
    Translation

    Target ORF information:

    RefSeq Version XM_014110234.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGGAGAAC TGCCCGGGGA GGAGGCCACG ACCATGGGGG ATGTGAAGCT GGCCGCCTTG 
    ACACAGGCTT CTAAAACCTC CGTCAGTGTG GATCACTCGA GGGCCGGGTC TGCACCCCCG
    ACAGAGGCCC CTGCTTTCAT TTTGCCCCCT CGGAACCTCT GCATCAGAGA AGGAGCCACC
    GCCAAGTTTG AAGGGAGGGT CCGGGGTTAC CCAGAGCCCC AGGTGACATG GCACCGAAAT
    GGGCAACCCA TCACCAGTGG GGGCCGCTTC CTGCTGGATT GTGGTATCCG GGGGACTTTC
    AGCCTCGTGA TTCATGCTGT CCGTGAGGAG GACCAGGGAA AGTATACCTG CGAAGCCACC
    AATGATGGTG GTGCCCGCCA GGTGACAGTG GAGTTGACTG TAGAAGGGGG CTTCATGAAG
    AAGCATGGTC AGCCCGTTGT TTCCAAAACC TTGGGGGACA GATTTGCAGC CCCCGCAGTG
    GAGACCCGTC CTAGCATCTG GGGAGAGTGC CCACCAAAGT TTGCTACCAA GTTGGGCCGA
    GCCGTAGTCA AAGAAGGACA GACGGGACGG TTCTCCTGCA AGATCACTGG ACGGCCCCAA
    CCACAGGTCA CCTGGCTCAA GGGGGATGTT GCCCTGCAGC CAAGTGCCCG TGTGTCTATG
    TCTGAGAAGA ACGGGGTACA AGTTCTGGAA ATCCATGAGG TCAGCCGCGA TGATGTGGGA
    GTGTACACAT GCATGGTGGT GAATGGGTCG GGGAAGGCCT CCATGTCGGC TGAGCTTTCT
    ATCCAAGGTT TGGACAATAC CAGTAGGTCA TTAACAAAGG TGGCCAATTC AGATATCAGG
    AAGGAGGTGA CCAATGGAAC CACGCAGGGG CCGAAACTAG ACCACCTGGA GTCTGCAGCG
    GAAAGGTGGC CCTGCTCCAG CACCCAGATC GGTGGCTCCC CCACCTGGGC CACAAGTAGC
    CAGCCTCAGC CCCTGAGGGA GTCCAAGCTG GAGCCGCCTG GGGACCCACC CAGGAAAGCC
    CTGAGGAGCC CCGTCCTACA GAAGACTTCC AGCACCATTA CCCTGCAGGC GGCAAAAGTC
    CAGCCAGAGC CAAGAGCGCC GGTCTCAAGT GCCCTCTCGT CTCCCGGAGA AGAGAGGGAG
    AGGCCAGCTG CTCCCCCCAC AGCCACCTTC CCTGCCCGGC AGTCTGGCCT GGGAAGCCAG
    GAGGTTGTGA ACAAGGTTGC TACCAGAAAA ATCCCCATGG AAAGCCAGAG AGATTCCACA
    TTCCCCAAAT TTGAGAGCAA GCCCCAAAGC CAGGAGGTCA GTGAAGATGA AACAGTCAAG
    TTCAGATGTG AGGTTTCAGG GACCCCAAAG CCTGAAGTGA CCTGGTTCCT GGAAGGTGCC
    CCCGTGAAGA GACGAGAAGG CATGGTTGAG GTTTATGAAG AAGGTGGATG CCACTACCTC
    TGCTTGCTGA GAGTGCGGGC CAAGGACAGT GGGAGCTACA GTTGCACGGC CTCCAATGTC
    CGAGGCCAGG TGTCCTGTGG TTGGACCCTC CTGGTGAAAC GGCTGGCCGT GATGGAGGTG
    GCTCCCTCGT TCTCCAGCGT CCTGAAGGAC TGCGCTGTCA TTGAGGGCCA GGACTTTGTG
    CTGCAGTGCT CAGTGCAGGG GACCCCGGTG CCCCAAATCA CTTGGCTGCT TAATGAGCAG
    CCCATCCAGT ACGCTCACTC CTCCTGCGAG GCTGGCGTGG CCGAGCTCCA CATCCAGGAT
    GCCCTGCCGG AAGATGATGG CATTTACACC TGTCTGGCCA AGAACACCTT GGGCCAGGTG
    TCCTGCAGCG CCCGGGTCAC CGTCCATGAA AAGAAGAGCG ACAGGAAGAG TGGGTATCTT
    CTGCGCACAG CTCCCGGCAA GCCCGTCGCA CCCCTCTTCC TGCAGGGCCT CTCTGACCTC
    AAAGTCATGG ATGGAAGCCA GGTCACCATG ACAGTCCAGG TGTCAGGGAA TCCGCCCCCC
    GAGGTCATCT GGCTTCACAA CGGGAATGAG ATCCAGGAGT CAGAGGACTT CCACTTTGAA
    CAGAGAGGCA CTCAGCACAG CCTTTGTATC CAGGAAGTGT TCCCAGAGGA CACAGGCACT
    TACACCTGCG AAGCCTGGAA CAGTGCTGGA GAGGTCCGCA CCCAGGCAGT ACTGATGGTG
    CAAGAGCCCC AGGATGGCAC CCAGCCCTGG TTCATCAGCA AGCCCCGCTC ACTGACTGCC
    TCCCTGGGCC AGACTGTCCT CATCTCCTGT GCCATCGCTG GGGACCCCTT CCCCACTGTG
    CACTGGCTGC GAGATGGCAA AGCCCTCTCC AAAGACTCCG GCCACTTCGA GGTGCTACAG
    AACGAGGACG TGTTCACCCT GGTTCTAAAG AATGTGCAGC CCTGGCACGC CGGCCAGTAC
    GAGATCCAGC TCAAGAACCG GGTTGGCGAA TGCAGTTGCC AGGTGTCACT GATGCTACAG
    AGCAGCCCCA CCGGAGCCCC CCTGCGGGGG AGGGAGCCTG CCAGCTGCGA GGGCCTCTGC
    AGCCGGGGAG CCGGCGCTGA TGGTGGTGGC GGTGACTGCT ATGGGACCCT GAGGCCTGGC
    CGGCCAGCGA GAGGACAGGG TTGGCCAGAG GAGGAGGACG ACGGCGAGGA CGTGCGGGGG
    GTGCTGAAGA GGCGCGTGGA GACCCGGCAA CATACTGAGG AGGCCATCCG CCAGCAGCAG
    GTGGAACAGC TGGACTTCCG TGACCTCCTG GGGAAGAAGG TGAGTACCAA GACCGTGTCA
    GAAGAAGACC TGAAGGAGAT CCCAGCGGAG CAGATGGATT TCCGCGCCAA CCTGCAGCGG
    CAAGTGAAGC CAAAGACTGT GTCAGAGGAA GAGAGGAAGG TGCATAGCCC TCAGCAGGTC
    GACTTCCGCT CGGTCCTGGC CAAAAAGGGG ACTCCTAAGA CCCCGGTGCC TGAGAAGGCA
    CCTCTTCCCA AACCCGCCAC CCCAGATTTC CGCTCCGTGT TAGGTAGCAA GAAAAAATTA
    CCAGCCGAGA ATGGTAGCAA CAACGCTGAG GCCTTGAATG CCAAGGCAGC AGAAAGTCCC
    AAGGCTGTGA GCAATGCCCA GCCTTTAGGG TCCCTGAAAC CTTTGGGCAA TGCCAAGCCT
    GCTGAGACTC TAAAACCTGT GGGCAACATC AGGCCTGCTG AGACGCTGAA ACCCATAGGC
    AACGCCAAAC CTGCTGAGAC CCTGAAACCC ATGGGCAACG CCAAGCCTGC TGAGACGCTA
    AAACCCATAG GCAATGCCAA GCTTGCTGAG ACCCCGAAAC CCATGGGCAA TGCCAAGCCT
    GCTGAGACCC TGAAACCCCT GGGCAACGCC AAGCCTGCTG AGACGCTGAA ACCCATAAGC
    AACGCCAAGC CTGCTGAACC TCTGAAACCT GTGGGCAACG CCAAGCCTGC TGAGACCCCA
    AAACCCATAG GTAATGCCAA GCCTGCTGAG ATCCTAAAAC CAGCTGGGAA GGAAGAACTC
    AAGAAGGAGG TTAAGAATGA CGTGAACTGC AAGAGGGCAC AGGCAGGAGC CACAGATAAT
    GAAAAAAGAT CAGAGAACCA AGGGACAGCC CCAGCCTTCA AGGAGAAGCT GCAAGATGTC
    CATGTGACAG AGGGTGAGAA GCTGCTACTC CAGTGTCAGG TGTCGTCTGA CCCTCCAGCC
    ACCATCACCT GGATGCTCAA TGGAAAGACA CTCAAGACCA CCAAGTTCAT CATCCTCTCC
    CAAGAAGGCT CACTGTGCTC CGTGTCCATC GAGAAGGCCC TGCCCGAGGA CAGAGGCTTC
    TACAAGTGCA TAGCTAAGAA TGGCGCCGGC CAGGCTGAGT GCTCCTGCCA AGTCACCGTG
    GACGATGCTC CCACCAAGGA GAATGCCAAG GCCCCGGAGA TGAAAGCCCG GAGGCCCAAG
    AGCTCTCTAC CTCCCATGCT AGGAACAGAG AGTGATGCAA CTGTGAAAAA GAAACCTGCC
    CCCAAGACAC CTCCAAAAGC AGCCATGCCC CCTCAGATCA TCCAGTTTCC TGAGGACCAA
    AAGGTGCGCG CAGGAGAGTC TGTGGAGCTG TTTGGTAAAG TGGCAGGCAC CCAGCCCATC
    ACCTGTACGT GGATGAAGTT CCGAAAGCAG ATCCAGGAGA GCGAGCACAT CAAGGTGGAG
    ACCAGTGAGA ATGGCAGCAG GCTCACCATC GTGGCCGCAC GCCAGGAACA CTGCGGCTGC
    TACACTCTGC TGGTGGAGAA CAAGCTGGGC AGCAGGCAGG CCCAGGTCAA CCTCACTGTC
    GTGGACAAAC CAGACCCCCC AGCGGGCACA CCTTGCGCCT CCGACATCCG GAGCTCGTCG
    TTGACCCTGT CCTGGTATGG CTCCTCGTAT GATGGGGGCA GTGCCGTGCA ATCCTACAGC
    GTCGAGATCT GGGACTCGGT GGACAAGACG TGGAAGGAAC TAGCCACGTG CCGCAGCACC
    TCTTTTAACG TCCAGGACCT GCTGCCTGAC CGAGAGTATA AATTCCGCGT TCGCGCCATC
    AACGTGTATG GAACCAGTGA GCCAAGCCAG GAGTCTGAAC TCACAGCAGT GGGAGAGAAA
    CCGGAGGAGC CGAAGGACGA AGTGGAGGTG TCGGATGATG ATGAGAAGGA ACCTGAGGTC
    GACTACCGGA CAGTGACGGT CAACACTGAA CAAAAAGTGT CCGACTTCTA TGACATCGAA
    GAGAGACTAG GCTCTGGGAA ATTTGGACAG GTCTTCCGAC TTGTAGAAAA GAAAACTGGA
    AAAATCTGGG CAGGGAAATT CTTCAAGGCA TATTCAGCCA AAGAGAAGGA GAATATCCGG
    CAGGAGATTA GCATCATGAA CTGCCTCCAC CACCCCAAGC TGGTCCAGTG TGTGGATGCC
    TTCGAAGAAA AGGCCAACAT CGTCATGGTC CTGGAGATCG TGTCGGGGGG CGAGCTGTTC
    GAGCGCATCA TCGACGAGGA CTTCGAGCTG ACGGAGCGCG AGTGCATCCA GTACATGAGG
    CAGATCTCGG CGGGCGTGGA GTACATCCAC CGGCAGGGCA TCGTCCACCT GGACCTCAAG
    CCCGAGAACA TCATGTGCGT CAATAAGACC GGCACGAGGA TCAAGCTCAT CGACTTCGGC
    CTGGCCAGAA AGCTGGAGAA TGCGGGGTCT CTGAAGGTCC TCTTTGGTAC CCCGGAATTT
    GTGGCCCCCG AAGTGATCAA CTATGAGCCC ATCGGCTATG CCACGGACAT GTGGAGCATC
    GGAGTCATCT GCTACATCCT GGTTAGTGGA CTCTCCCCCT TCATGGGTGA CAACGATAAT
    GAAACCCTAG CCAACGTTAC CTCAGCCACC TGGGACTTCG ATGATGAGGC GTTCGATGAG
    ATCTCGGATG ACGCCAAGGA TTTTATCAGC AATTTGCTGA AGAAAGATAT GAAAAACCGC
    CTGGACTGTA CCCAGTGCCT TCAGCATCCC TGGCTCATGA AAGACACCAA GAACATGGAA
    GCCAAGAAAC TCTCCAAGGA CCGGATGAAG AAGTACATGG CAAGAAGGAA ATGGCAGAAA
    ACGGGCAATG CTGTGAGAGC CATCGGAAGA CTGTCCTCTA TGGCAATGAT CTCAGGGCTC
    AGTGGCAGGA AATCCTCAAC AGGATCACCG ACCAGCCCGC TCAACGCAGA AAAACTAGAA
    TCTGAAGATG TGTCCCAAGC TTTCCTTGAG GCTGTTGCTG AGGAAAAGCC CCATGTAAAA
    CCCTATTTCT CTAAGACCAT TCGTGATTTA GAAGTTGTGG AGGGAAGCGC TGCTAGATTT
    GACTGCAAGA TTGAAGGATA CCCAGACCCT GAAGTTGTCT GGTTTAAAGA TGACCAGTCA
    ATCCGGGAGT CCCGCCACTT CCAGATAGAC TACGACGAGG ATGGGAACTG CTCTTTAATC
    ATTAGCGATG TTTGTGGGGA TGACGATGCC AAGTACACCT GCAAGGCTGT CAATAGTCTT
    GGAGAAGCCA CTTGCACAGC AGAGCTCATT GTGGAGACCA TGGAGGAAGG GGAAGGGGAA
    GGGGAAGAGG AAGAAGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44725
    Clone ID Related Accession (Same CDS sequence) XM_014110235.2 , XM_014110235.1
    Accession Version XM_014110235.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006615.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110235.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGGGGATG TGAAGCTGGC CGCCTTGACA CAGGCTTCTA AAACCTCCGT CAGTGTGGAT 
    CACTCGAGGG CCGGGTCTGC ACCCCCGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAGAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CCGAAATGGG CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGATTGTG GTATCCGGGG GACTTTCAGC CTCGTGATTC ATGCTGTCCG TGAGGAGGAC
    CAGGGAAAGT ATACCTGCGA AGCCACCAAT GATGGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACTGTAG AAGGGGGCTT CATGAAGAAG CATGGTCAGC CCGTTGTTTC CAAAACCTTG
    GGGGACAGAT TTGCAGCCCC CGCAGTGGAG ACCCGTCCTA GCATCTGGGG AGAGTGCCCA
    CCAAAGTTTG CTACCAAGTT GGGCCGAGCC GTAGTCAAAG AAGGACAGAC GGGACGGTTC
    TCCTGCAAGA TCACTGGACG GCCCCAACCA CAGGTCACCT GGCTCAAGGG GGATGTTGCC
    CTGCAGCCAA GTGCCCGTGT GTCTATGTCT GAGAAGAACG GGGTACAAGT TCTGGAAATC
    CATGAGGTCA GCCGCGATGA TGTGGGAGTG TACACATGCA TGGTGGTGAA TGGGTCGGGG
    AAGGCCTCCA TGTCGGCTGA GCTTTCTATC CAAGGTTTGG ACAATACCAG TAGGTCATTA
    ACAAAGGTGG CCAATTCAGA TATCAGGAAG GAGGTGACCA ATGGAACCAC GCAGGGGCCG
    AAACTAGACC ACCTGGAGTC TGCAGCGGAA AGGTGGCCCT GCTCCAGCAC CCAGATCGGT
    GGCTCCCCCA CCTGGGCCAC AAGTAGCCAG CCTCAGCCCC TGAGGGAGTC CAAGCTGGAG
    CCGCCTGGGG ACCCACCCAG GAAAGCCCTG AGGAGCCCCG TCCTACAGAA GACTTCCAGC
    ACCATTACCC TGCAGGCGGC AAAAGTCCAG CCAGAGCCAA GAGCGCCGGT CTCAAGTGCC
    CTCTCGTCTC CCGGAGAAGA GAGGGAGAGG CCAGCTGCTC CCCCCACAGC CACCTTCCCT
    GCCCGGCAGT CTGGCCTGGG AAGCCAGGAG GTTGTGAACA AGGTTGCTAC CAGAAAAATC
    CCCATGGAAA GCCAGAGAGA TTCCACATTC CCCAAATTTG AGAGCAAGCC CCAAAGCCAG
    GAGGTCAGTG AAGATGAAAC AGTCAAGTTC AGATGTGAGG TTTCAGGGAC CCCAAAGCCT
    GAAGTGACCT GGTTCCTGGA AGGTGCCCCC GTGAAGAGAC GAGAAGGCAT GGTTGAGGTT
    TATGAAGAAG GTGGATGCCA CTACCTCTGC TTGCTGAGAG TGCGGGCCAA GGACAGTGGG
    AGCTACAGTT GCACGGCCTC CAATGTCCGA GGCCAGGTGT CCTGTGGTTG GACCCTCCTG
    GTGAAACGGC TGGCCGTGAT GGAGGTGGCT CCCTCGTTCT CCAGCGTCCT GAAGGACTGC
    GCTGTCATTG AGGGCCAGGA CTTTGTGCTG CAGTGCTCAG TGCAGGGGAC CCCGGTGCCC
    CAAATCACTT GGCTGCTTAA TGAGCAGCCC ATCCAGTACG CTCACTCCTC CTGCGAGGCT
    GGCGTGGCCG AGCTCCACAT CCAGGATGCC CTGCCGGAAG ATGATGGCAT TTACACCTGT
    CTGGCCAAGA ACACCTTGGG CCAGGTGTCC TGCAGCGCCC GGGTCACCGT CCATGAAAAG
    AAGAGCGACA GGAAGAGTGG GTATCTTCTG CGCACAGCTC CCGGCAAGCC CGTCGCACCC
    CTCTTCCTGC AGGGCCTCTC TGACCTCAAA GTCATGGATG GAAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGGAATCC GCCCCCCGAG GTCATCTGGC TTCACAACGG GAATGAGATC
    CAGGAGTCAG AGGACTTCCA CTTTGAACAG AGAGGCACTC AGCACAGCCT TTGTATCCAG
    GAAGTGTTCC CAGAGGACAC AGGCACTTAC ACCTGCGAAG CCTGGAACAG TGCTGGAGAG
    GTCCGCACCC AGGCAGTACT GATGGTGCAA GAGCCCCAGG ATGGCACCCA GCCCTGGTTC
    ATCAGCAAGC CCCGCTCACT GACTGCCTCC CTGGGCCAGA CTGTCCTCAT CTCCTGTGCC
    ATCGCTGGGG ACCCCTTCCC CACTGTGCAC TGGCTGCGAG ATGGCAAAGC CCTCTCCAAA
    GACTCCGGCC ACTTCGAGGT GCTACAGAAC GAGGACGTGT TCACCCTGGT TCTAAAGAAT
    GTGCAGCCCT GGCACGCCGG CCAGTACGAG ATCCAGCTCA AGAACCGGGT TGGCGAATGC
    AGTTGCCAGG TGTCACTGAT GCTACAGAGC AGCCCCACCG GAGCCCCCCT GCGGGGGAGG
    GAGCCTGCCA GCTGCGAGGG CCTCTGCAGC CGGGGAGCCG GCGCTGATGG TGGTGGCGGT
    GACTGCTATG GGACCCTGAG GCCTGGCCGG CCAGCGAGAG GACAGGGTTG GCCAGAGGAG
    GAGGACGACG GCGAGGACGT GCGGGGGGTG CTGAAGAGGC GCGTGGAGAC CCGGCAACAT
    ACTGAGGAGG CCATCCGCCA GCAGCAGGTG GAACAGCTGG ACTTCCGTGA CCTCCTGGGG
    AAGAAGGTGA GTACCAAGAC CGTGTCAGAA GAAGACCTGA AGGAGATCCC AGCGGAGCAG
    ATGGATTTCC GCGCCAACCT GCAGCGGCAA GTGAAGCCAA AGACTGTGTC AGAGGAAGAG
    AGGAAGGTGC ATAGCCCTCA GCAGGTCGAC TTCCGCTCGG TCCTGGCCAA AAAGGGGACT
    CCTAAGACCC CGGTGCCTGA GAAGGCACCT CTTCCCAAAC CCGCCACCCC AGATTTCCGC
    TCCGTGTTAG GTAGCAAGAA AAAATTACCA GCCGAGAATG GTAGCAACAA CGCTGAGGCC
    TTGAATGCCA AGGCAGCAGA AAGTCCCAAG GCTGTGAGCA ATGCCCAGCC TTTAGGGTCC
    CTGAAACCTT TGGGCAATGC CAAGCCTGCT GAGACTCTAA AACCTGTGGG CAACATCAGG
    CCTGCTGAGA CGCTGAAACC CATAGGCAAC GCCAAACCTG CTGAGACCCT GAAACCCATG
    GGCAACGCCA AGCCTGCTGA GACGCTAAAA CCCATAGGCA ATGCCAAGCT TGCTGAGACC
    CCGAAACCCA TGGGCAATGC CAAGCCTGCT GAGACCCTGA AACCCCTGGG CAACGCCAAG
    CCTGCTGAGA CGCTGAAACC CATAAGCAAC GCCAAGCCTG CTGAACCTCT GAAACCTGTG
    GGCAACGCCA AGCCTGCTGA GACCCCAAAA CCCATAGGTA ATGCCAAGCC TGCTGAGATC
    CTAAAACCAG CTGGGAAGGA AGAACTCAAG AAGGAGGTTA AGAATGACGT GAACTGCAAG
    AGGGCACAGG CAGGAGCCAC AGATAATGAA AAAAGATCAG AGAACCAAGG GACAGCCCCA
    GCCTTCAAGG AGAAGCTGCA AGATGTCCAT GTGACAGAGG GTGAGAAGCT GCTACTCCAG
    TGTCAGGTGT CGTCTGACCC TCCAGCCACC ATCACCTGGA TGCTCAATGG AAAGACACTC
    AAGACCACCA AGTTCATCAT CCTCTCCCAA GAAGGCTCAC TGTGCTCCGT GTCCATCGAG
    AAGGCCCTGC CCGAGGACAG AGGCTTCTAC AAGTGCATAG CTAAGAATGG CGCCGGCCAG
    GCTGAGTGCT CCTGCCAAGT CACCGTGGAC GATGCTCCCA CCAAGGAGAA TGCCAAGGCC
    CCGGAGATGA AAGCCCGGAG GCCCAAGAGC TCTCTACCTC CCATGCTAGG AACAGAGAGT
    GATGCAACTG TGAAAAAGAA ACCTGCCCCC AAGACACCTC CAAAAGCAGC CATGCCCCCT
    CAGATCATCC AGTTTCCTGA GGACCAAAAG GTGCGCGCAG GAGAGTCTGT GGAGCTGTTT
    GGTAAAGTGG CAGGCACCCA GCCCATCACC TGTACGTGGA TGAAGTTCCG AAAGCAGATC
    CAGGAGAGCG AGCACATCAA GGTGGAGACC AGTGAGAATG GCAGCAGGCT CACCATCGTG
    GCCGCACGCC AGGAACACTG CGGCTGCTAC ACTCTGCTGG TGGAGAACAA GCTGGGCAGC
    AGGCAGGCCC AGGTCAACCT CACTGTCGTG GACAAACCAG ACCCCCCAGC GGGCACACCT
    TGCGCCTCCG ACATCCGGAG CTCGTCGTTG ACCCTGTCCT GGTATGGCTC CTCGTATGAT
    GGGGGCAGTG CCGTGCAATC CTACAGCGTC GAGATCTGGG ACTCGGTGGA CAAGACGTGG
    AAGGAACTAG CCACGTGCCG CAGCACCTCT TTTAACGTCC AGGACCTGCT GCCTGACCGA
    GAGTATAAAT TCCGCGTTCG CGCCATCAAC GTGTATGGAA CCAGTGAGCC AAGCCAGGAG
    TCTGAACTCA CAGCAGTGGG AGAGAAACCG GAGGAGCCGA AGGACGAAGT GGAGGTGTCG
    GATGATGATG AGAAGGAACC TGAGGTCGAC TACCGGACAG TGACGGTCAA CACTGAACAA
    AAAGTGTCCG ACTTCTATGA CATCGAAGAG AGACTAGGCT CTGGGAAATT TGGACAGGTC
    TTCCGACTTG TAGAAAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTCTT CAAGGCATAT
    TCAGCCAAAG AGAAGGAGAA TATCCGGCAG GAGATTAGCA TCATGAACTG CCTCCACCAC
    CCCAAGCTGG TCCAGTGTGT GGATGCCTTC GAAGAAAAGG CCAACATCGT CATGGTCCTG
    GAGATCGTGT CGGGGGGCGA GCTGTTCGAG CGCATCATCG ACGAGGACTT CGAGCTGACG
    GAGCGCGAGT GCATCCAGTA CATGAGGCAG ATCTCGGCGG GCGTGGAGTA CATCCACCGG
    CAGGGCATCG TCCACCTGGA CCTCAAGCCC GAGAACATCA TGTGCGTCAA TAAGACCGGC
    ACGAGGATCA AGCTCATCGA CTTCGGCCTG GCCAGAAAGC TGGAGAATGC GGGGTCTCTG
    AAGGTCCTCT TTGGTACCCC GGAATTTGTG GCCCCCGAAG TGATCAACTA TGAGCCCATC
    GGCTATGCCA CGGACATGTG GAGCATCGGA GTCATCTGCT ACATCCTGGT TAGTGGACTC
    TCCCCCTTCA TGGGTGACAA CGATAATGAA ACCCTAGCCA ACGTTACCTC AGCCACCTGG
    GACTTCGATG ATGAGGCGTT CGATGAGATC TCGGATGACG CCAAGGATTT TATCAGCAAT
    TTGCTGAAGA AAGATATGAA AAACCGCCTG GACTGTACCC AGTGCCTTCA GCATCCCTGG
    CTCATGAAAG ACACCAAGAA CATGGAAGCC AAGAAACTCT CCAAGGACCG GATGAAGAAG
    TACATGGCAA GAAGGAAATG GCAGAAAACG GGCAATGCTG TGAGAGCCAT CGGAAGACTG
    TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CCTCAACAGG ATCACCGACC
    AGCCCGCTCA ACGCAGAAAA ACTAGAATCT GAAGAAGATG TGTCCCAAGC TTTCCTTGAG
    GCTGTTGCTG AGGAAAAGCC CCATGTAAAA CCCTATTTCT CTAAGACCAT TCGTGATTTA
    GAAGTTGTGG AGGGAAGCGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCT
    GAAGTTGTCT GGTTTAAAGA TGACCAGTCA ATCCGGGAGT CCCGCCACTT CCAGATAGAC
    TACGACGAGG ATGGGAACTG CTCTTTAATC ATTAGCGATG TTTGTGGGGA TGACGATGCC
    AAGTACACCT GCAAGGCTGT CAATAGTCTT GGAGAAGCCA CTTGCACAGC AGAGCTCATT
    GTGGAGACCA TGGAGGAAGG GGAAGGGGAA GGGGAAGAGG AAGAAGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965710.1
    CDS140..6136
    Translation

    Target ORF information:

    RefSeq Version XM_014110235.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110235.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGGGGATG TGAAGCTGGC CGCCTTGACA CAGGCTTCTA AAACCTCCGT CAGTGTGGAT 
    CACTCGAGGG CCGGGTCTGC ACCCCCGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAGAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CCGAAATGGG CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGATTGTG GTATCCGGGG GACTTTCAGC CTCGTGATTC ATGCTGTCCG TGAGGAGGAC
    CAGGGAAAGT ATACCTGCGA AGCCACCAAT GATGGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACTGTAG AAGGGGGCTT CATGAAGAAG CATGGTCAGC CCGTTGTTTC CAAAACCTTG
    GGGGACAGAT TTGCAGCCCC CGCAGTGGAG ACCCGTCCTA GCATCTGGGG AGAGTGCCCA
    CCAAAGTTTG CTACCAAGTT GGGCCGAGCC GTAGTCAAAG AAGGACAGAC GGGACGGTTC
    TCCTGCAAGA TCACTGGACG GCCCCAACCA CAGGTCACCT GGCTCAAGGG GGATGTTGCC
    CTGCAGCCAA GTGCCCGTGT GTCTATGTCT GAGAAGAACG GGGTACAAGT TCTGGAAATC
    CATGAGGTCA GCCGCGATGA TGTGGGAGTG TACACATGCA TGGTGGTGAA TGGGTCGGGG
    AAGGCCTCCA TGTCGGCTGA GCTTTCTATC CAAGGTTTGG ACAATACCAG TAGGTCATTA
    ACAAAGGTGG CCAATTCAGA TATCAGGAAG GAGGTGACCA ATGGAACCAC GCAGGGGCCG
    AAACTAGACC ACCTGGAGTC TGCAGCGGAA AGGTGGCCCT GCTCCAGCAC CCAGATCGGT
    GGCTCCCCCA CCTGGGCCAC AAGTAGCCAG CCTCAGCCCC TGAGGGAGTC CAAGCTGGAG
    CCGCCTGGGG ACCCACCCAG GAAAGCCCTG AGGAGCCCCG TCCTACAGAA GACTTCCAGC
    ACCATTACCC TGCAGGCGGC AAAAGTCCAG CCAGAGCCAA GAGCGCCGGT CTCAAGTGCC
    CTCTCGTCTC CCGGAGAAGA GAGGGAGAGG CCAGCTGCTC CCCCCACAGC CACCTTCCCT
    GCCCGGCAGT CTGGCCTGGG AAGCCAGGAG GTTGTGAACA AGGTTGCTAC CAGAAAAATC
    CCCATGGAAA GCCAGAGAGA TTCCACATTC CCCAAATTTG AGAGCAAGCC CCAAAGCCAG
    GAGGTCAGTG AAGATGAAAC AGTCAAGTTC AGATGTGAGG TTTCAGGGAC CCCAAAGCCT
    GAAGTGACCT GGTTCCTGGA AGGTGCCCCC GTGAAGAGAC GAGAAGGCAT GGTTGAGGTT
    TATGAAGAAG GTGGATGCCA CTACCTCTGC TTGCTGAGAG TGCGGGCCAA GGACAGTGGG
    AGCTACAGTT GCACGGCCTC CAATGTCCGA GGCCAGGTGT CCTGTGGTTG GACCCTCCTG
    GTGAAACGGC TGGCCGTGAT GGAGGTGGCT CCCTCGTTCT CCAGCGTCCT GAAGGACTGC
    GCTGTCATTG AGGGCCAGGA CTTTGTGCTG CAGTGCTCAG TGCAGGGGAC CCCGGTGCCC
    CAAATCACTT GGCTGCTTAA TGAGCAGCCC ATCCAGTACG CTCACTCCTC CTGCGAGGCT
    GGCGTGGCCG AGCTCCACAT CCAGGATGCC CTGCCGGAAG ATGATGGCAT TTACACCTGT
    CTGGCCAAGA ACACCTTGGG CCAGGTGTCC TGCAGCGCCC GGGTCACCGT CCATGAAAAG
    AAGAGCGACA GGAAGAGTGG GTATCTTCTG CGCACAGCTC CCGGCAAGCC CGTCGCACCC
    CTCTTCCTGC AGGGCCTCTC TGACCTCAAA GTCATGGATG GAAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGGAATCC GCCCCCCGAG GTCATCTGGC TTCACAACGG GAATGAGATC
    CAGGAGTCAG AGGACTTCCA CTTTGAACAG AGAGGCACTC AGCACAGCCT TTGTATCCAG
    GAAGTGTTCC CAGAGGACAC AGGCACTTAC ACCTGCGAAG CCTGGAACAG TGCTGGAGAG
    GTCCGCACCC AGGCAGTACT GATGGTGCAA GAGCCCCAGG ATGGCACCCA GCCCTGGTTC
    ATCAGCAAGC CCCGCTCACT GACTGCCTCC CTGGGCCAGA CTGTCCTCAT CTCCTGTGCC
    ATCGCTGGGG ACCCCTTCCC CACTGTGCAC TGGCTGCGAG ATGGCAAAGC CCTCTCCAAA
    GACTCCGGCC ACTTCGAGGT GCTACAGAAC GAGGACGTGT TCACCCTGGT TCTAAAGAAT
    GTGCAGCCCT GGCACGCCGG CCAGTACGAG ATCCAGCTCA AGAACCGGGT TGGCGAATGC
    AGTTGCCAGG TGTCACTGAT GCTACAGAGC AGCCCCACCG GAGCCCCCCT GCGGGGGAGG
    GAGCCTGCCA GCTGCGAGGG CCTCTGCAGC CGGGGAGCCG GCGCTGATGG TGGTGGCGGT
    GACTGCTATG GGACCCTGAG GCCTGGCCGG CCAGCGAGAG GACAGGGTTG GCCAGAGGAG
    GAGGACGACG GCGAGGACGT GCGGGGGGTG CTGAAGAGGC GCGTGGAGAC CCGGCAACAT
    ACTGAGGAGG CCATCCGCCA GCAGCAGGTG GAACAGCTGG ACTTCCGTGA CCTCCTGGGG
    AAGAAGGTGA GTACCAAGAC CGTGTCAGAA GAAGACCTGA AGGAGATCCC AGCGGAGCAG
    ATGGATTTCC GCGCCAACCT GCAGCGGCAA GTGAAGCCAA AGACTGTGTC AGAGGAAGAG
    AGGAAGGTGC ATAGCCCTCA GCAGGTCGAC TTCCGCTCGG TCCTGGCCAA AAAGGGGACT
    CCTAAGACCC CGGTGCCTGA GAAGGCACCT CTTCCCAAAC CCGCCACCCC AGATTTCCGC
    TCCGTGTTAG GTAGCAAGAA AAAATTACCA GCCGAGAATG GTAGCAACAA CGCTGAGGCC
    TTGAATGCCA AGGCAGCAGA AAGTCCCAAG GCTGTGAGCA ATGCCCAGCC TTTAGGGTCC
    CTGAAACCTT TGGGCAATGC CAAGCCTGCT GAGACTCTAA AACCTGTGGG CAACATCAGG
    CCTGCTGAGA CGCTGAAACC CATAGGCAAC GCCAAACCTG CTGAGACCCT GAAACCCATG
    GGCAACGCCA AGCCTGCTGA GACGCTAAAA CCCATAGGCA ATGCCAAGCT TGCTGAGACC
    CCGAAACCCA TGGGCAATGC CAAGCCTGCT GAGACCCTGA AACCCCTGGG CAACGCCAAG
    CCTGCTGAGA CGCTGAAACC CATAAGCAAC GCCAAGCCTG CTGAACCTCT GAAACCTGTG
    GGCAACGCCA AGCCTGCTGA GACCCCAAAA CCCATAGGTA ATGCCAAGCC TGCTGAGATC
    CTAAAACCAG CTGGGAAGGA AGAACTCAAG AAGGAGGTTA AGAATGACGT GAACTGCAAG
    AGGGCACAGG CAGGAGCCAC AGATAATGAA AAAAGATCAG AGAACCAAGG GACAGCCCCA
    GCCTTCAAGG AGAAGCTGCA AGATGTCCAT GTGACAGAGG GTGAGAAGCT GCTACTCCAG
    TGTCAGGTGT CGTCTGACCC TCCAGCCACC ATCACCTGGA TGCTCAATGG AAAGACACTC
    AAGACCACCA AGTTCATCAT CCTCTCCCAA GAAGGCTCAC TGTGCTCCGT GTCCATCGAG
    AAGGCCCTGC CCGAGGACAG AGGCTTCTAC AAGTGCATAG CTAAGAATGG CGCCGGCCAG
    GCTGAGTGCT CCTGCCAAGT CACCGTGGAC GATGCTCCCA CCAAGGAGAA TGCCAAGGCC
    CCGGAGATGA AAGCCCGGAG GCCCAAGAGC TCTCTACCTC CCATGCTAGG AACAGAGAGT
    GATGCAACTG TGAAAAAGAA ACCTGCCCCC AAGACACCTC CAAAAGCAGC CATGCCCCCT
    CAGATCATCC AGTTTCCTGA GGACCAAAAG GTGCGCGCAG GAGAGTCTGT GGAGCTGTTT
    GGTAAAGTGG CAGGCACCCA GCCCATCACC TGTACGTGGA TGAAGTTCCG AAAGCAGATC
    CAGGAGAGCG AGCACATCAA GGTGGAGACC AGTGAGAATG GCAGCAGGCT CACCATCGTG
    GCCGCACGCC AGGAACACTG CGGCTGCTAC ACTCTGCTGG TGGAGAACAA GCTGGGCAGC
    AGGCAGGCCC AGGTCAACCT CACTGTCGTG GACAAACCAG ACCCCCCAGC GGGCACACCT
    TGCGCCTCCG ACATCCGGAG CTCGTCGTTG ACCCTGTCCT GGTATGGCTC CTCGTATGAT
    GGGGGCAGTG CCGTGCAATC CTACAGCGTC GAGATCTGGG ACTCGGTGGA CAAGACGTGG
    AAGGAACTAG CCACGTGCCG CAGCACCTCT TTTAACGTCC AGGACCTGCT GCCTGACCGA
    GAGTATAAAT TCCGCGTTCG CGCCATCAAC GTGTATGGAA CCAGTGAGCC AAGCCAGGAG
    TCTGAACTCA CAGCAGTGGG AGAGAAACCG GAGGAGCCGA AGGACGAAGT GGAGGTGTCG
    GATGATGATG AGAAGGAACC TGAGGTCGAC TACCGGACAG TGACGGTCAA CACTGAACAA
    AAAGTGTCCG ACTTCTATGA CATCGAAGAG AGACTAGGCT CTGGGAAATT TGGACAGGTC
    TTCCGACTTG TAGAAAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTCTT CAAGGCATAT
    TCAGCCAAAG AGAAGGAGAA TATCCGGCAG GAGATTAGCA TCATGAACTG CCTCCACCAC
    CCCAAGCTGG TCCAGTGTGT GGATGCCTTC GAAGAAAAGG CCAACATCGT CATGGTCCTG
    GAGATCGTGT CGGGGGGCGA GCTGTTCGAG CGCATCATCG ACGAGGACTT CGAGCTGACG
    GAGCGCGAGT GCATCCAGTA CATGAGGCAG ATCTCGGCGG GCGTGGAGTA CATCCACCGG
    CAGGGCATCG TCCACCTGGA CCTCAAGCCC GAGAACATCA TGTGCGTCAA TAAGACCGGC
    ACGAGGATCA AGCTCATCGA CTTCGGCCTG GCCAGAAAGC TGGAGAATGC GGGGTCTCTG
    AAGGTCCTCT TTGGTACCCC GGAATTTGTG GCCCCCGAAG TGATCAACTA TGAGCCCATC
    GGCTATGCCA CGGACATGTG GAGCATCGGA GTCATCTGCT ACATCCTGGT TAGTGGACTC
    TCCCCCTTCA TGGGTGACAA CGATAATGAA ACCCTAGCCA ACGTTACCTC AGCCACCTGG
    GACTTCGATG ATGAGGCGTT CGATGAGATC TCGGATGACG CCAAGGATTT TATCAGCAAT
    TTGCTGAAGA AAGATATGAA AAACCGCCTG GACTGTACCC AGTGCCTTCA GCATCCCTGG
    CTCATGAAAG ACACCAAGAA CATGGAAGCC AAGAAACTCT CCAAGGACCG GATGAAGAAG
    TACATGGCAA GAAGGAAATG GCAGAAAACG GGCAATGCTG TGAGAGCCAT CGGAAGACTG
    TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CCTCAACAGG ATCACCGACC
    AGCCCGCTCA ACGCAGAAAA ACTAGAATCT GAAGAAGATG TGTCCCAAGC TTTCCTTGAG
    GCTGTTGCTG AGGAAAAGCC CCATGTAAAA CCCTATTTCT CTAAGACCAT TCGTGATTTA
    GAAGTTGTGG AGGGAAGCGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCT
    GAAGTTGTCT GGTTTAAAGA TGACCAGTCA ATCCGGGAGT CCCGCCACTT CCAGATAGAC
    TACGACGAGG ATGGGAACTG CTCTTTAATC ATTAGCGATG TTTGTGGGGA TGACGATGCC
    AAGTACACCT GCAAGGCTGT CAATAGTCTT GGAGAAGCCA CTTGCACAGC AGAGCTCATT
    GTGGAGACCA TGGAGGAAGG GGAAGGGGAA GGGGAAGAGG AAGAAGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44725
    Clone ID Related Accession (Same CDS sequence) XM_014110235.2 , XM_014110235.1
    Accession Version XM_014110235.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726118.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGGGGATG TGAAGCTGGC CGCCTTGACA CAGGCTTCTA AAACCTCCGT CAGTGTGGAT 
    CACTCGAGGG CCGGGTCTGC ACCCCCGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAGAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CCGAAATGGG CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGATTGTG GTATCCGGGG GACTTTCAGC CTCGTGATTC ATGCTGTCCG TGAGGAGGAC
    CAGGGAAAGT ATACCTGCGA AGCCACCAAT GATGGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACTGTAG AAGGGGGCTT CATGAAGAAG CATGGTCAGC CCGTTGTTTC CAAAACCTTG
    GGGGACAGAT TTGCAGCCCC CGCAGTGGAG ACCCGTCCTA GCATCTGGGG AGAGTGCCCA
    CCAAAGTTTG CTACCAAGTT GGGCCGAGCC GTAGTCAAAG AAGGACAGAC GGGACGGTTC
    TCCTGCAAGA TCACTGGACG GCCCCAACCA CAGGTCACCT GGCTCAAGGG GGATGTTGCC
    CTGCAGCCAA GTGCCCGTGT GTCTATGTCT GAGAAGAACG GGGTACAAGT TCTGGAAATC
    CATGAGGTCA GCCGCGATGA TGTGGGAGTG TACACATGCA TGGTGGTGAA TGGGTCGGGG
    AAGGCCTCCA TGTCGGCTGA GCTTTCTATC CAAGGTTTGG ACAATACCAG TAGGTCATTA
    ACAAAGGTGG CCAATTCAGA TATCAGGAAG GAGGTGACCA ATGGAACCAC GCAGGGGCCG
    AAACTAGACC ACCTGGAGTC TGCAGCGGAA AGGTGGCCCT GCTCCAGCAC CCAGATCGGT
    GGCTCCCCCA CCTGGGCCAC AAGTAGCCAG CCTCAGCCCC TGAGGGAGTC CAAGCTGGAG
    CCGCCTGGGG ACCCACCCAG GAAAGCCCTG AGGAGCCCCG TCCTACAGAA GACTTCCAGC
    ACCATTACCC TGCAGGCGGC AAAAGTCCAG CCAGAGCCAA GAGCGCCGGT CTCAAGTGCC
    CTCTCGTCTC CCGGAGAAGA GAGGGAGAGG CCAGCTGCTC CCCCCACAGC CACCTTCCCT
    GCCCGGCAGT CTGGCCTGGG AAGCCAGGAG GTTGTGAACA AGGTTGCTAC CAGAAAAATC
    CCCATGGAAA GCCAGAGAGA TTCCACATTC CCCAAATTTG AGAGCAAGCC CCAAAGCCAG
    GAGGTCAGTG AAGATGAAAC AGTCAAGTTC AGATGTGAGG TTTCAGGGAC CCCAAAGCCT
    GAAGTGACCT GGTTCCTGGA AGGTGCCCCC GTGAAGAGAC GAGAAGGCAT GGTTGAGGTT
    TATGAAGAAG GTGGATGCCA CTACCTCTGC TTGCTGAGAG TGCGGGCCAA GGACAGTGGG
    AGCTACAGTT GCACGGCCTC CAATGTCCGA GGCCAGGTGT CCTGTGGTTG GACCCTCCTG
    GTGAAACGGC TGGCCGTGAT GGAGGTGGCT CCCTCGTTCT CCAGCGTCCT GAAGGACTGC
    GCTGTCATTG AGGGCCAGGA CTTTGTGCTG CAGTGCTCAG TGCAGGGGAC CCCGGTGCCC
    CAAATCACTT GGCTGCTTAA TGAGCAGCCC ATCCAGTACG CTCACTCCTC CTGCGAGGCT
    GGCGTGGCCG AGCTCCACAT CCAGGATGCC CTGCCGGAAG ATGATGGCAT TTACACCTGT
    CTGGCCAAGA ACACCTTGGG CCAGGTGTCC TGCAGCGCCC GGGTCACCGT CCATGAAAAG
    AAGAGCGACA GGAAGAGTGG GTATCTTCTG CGCACAGCTC CCGGCAAGCC CGTCGCACCC
    CTCTTCCTGC AGGGCCTCTC TGACCTCAAA GTCATGGATG GAAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGGAATCC GCCCCCCGAG GTCATCTGGC TTCACAACGG GAATGAGATC
    CAGGAGTCAG AGGACTTCCA CTTTGAACAG AGAGGCACTC AGCACAGCCT TTGTATCCAG
    GAAGTGTTCC CAGAGGACAC AGGCACTTAC ACCTGCGAAG CCTGGAACAG TGCTGGAGAG
    GTCCGCACCC AGGCAGTACT GATGGTGCAA GAGCCCCAGG ATGGCACCCA GCCCTGGTTC
    ATCAGCAAGC CCCGCTCACT GACTGCCTCC CTGGGCCAGA CTGTCCTCAT CTCCTGTGCC
    ATCGCTGGGG ACCCCTTCCC CACTGTGCAC TGGCTGCGAG ATGGCAAAGC CCTCTCCAAA
    GACTCCGGCC ACTTCGAGGT GCTACAGAAC GAGGACGTGT TCACCCTGGT TCTAAAGAAT
    GTGCAGCCCT GGCACGCCGG CCAGTACGAG ATCCAGCTCA AGAACCGGGT TGGCGAATGC
    AGTTGCCAGG TGTCACTGAT GCTACAGAGC AGCCCCACCG GAGCCCCCCT GCGGGGGAGG
    GAGCCTGCCA GCTGCGAGGG CCTCTGCAGC CGGGGAGCCG GCGCTGATGG TGGTGGCGGT
    GACTGCTATG GGACCCTGAG GCCTGGCCGG CCAGCGAGAG GACAGGGTTG GCCAGAGGAG
    GAGGACGACG GCGAGGACGT GCGGGGGGTG CTGAAGAGGC GCGTGGAGAC CCGGCAACAT
    ACTGAGGAGG CCATCCGCCA GCAGCAGGTG GAACAGCTGG ACTTCCGTGA CCTCCTGGGG
    AAGAAGGTGA GTACCAAGAC CGTGTCAGAA GAAGACCTGA AGGAGATCCC AGCGGAGCAG
    ATGGATTTCC GCGCCAACCT GCAGCGGCAA GTGAAGCCAA AGACTGTGTC AGAGGAAGAG
    AGGAAGGTGC ATAGCCCTCA GCAGGTCGAC TTCCGCTCGG TCCTGGCCAA AAAGGGGACT
    CCTAAGACCC CGGTGCCTGA GAAGGCACCT CTTCCCAAAC CCGCCACCCC AGATTTCCGC
    TCCGTGTTAG GTAGCAAGAA AAAATTACCA GCCGAGAATG GTAGCAACAA CGCTGAGGCC
    TTGAATGCCA AGGCAGCAGA AAGTCCCAAG GCTGTGAGCA ATGCCCAGCC TTTAGGGTCC
    CTGAAACCTT TGGGCAATGC CAAGCCTGCT GAGACTCTAA AACCTGTGGG CAACATCAGG
    CCTGCTGAGA CGCTGAAACC CATAGGCAAC GCCAAACCTG CTGAGACCCT GAAACCCATG
    GGCAACGCCA AGCCTGCTGA GACGCTAAAA CCCATAGGCA ATGCCAAGCT TGCTGAGACC
    CCGAAACCCA TGGGCAATGC CAAGCCTGCT GAGACCCTGA AACCCCTGGG CAACGCCAAG
    CCTGCTGAGA CGCTGAAACC CATAAGCAAC GCCAAGCCTG CTGAACCTCT GAAACCTGTG
    GGCAACGCCA AGCCTGCTGA GACCCCAAAA CCCATAGGTA ATGCCAAGCC TGCTGAGATC
    CTAAAACCAG CTGGGAAGGA AGAACTCAAG AAGGAGGTTA AGAATGACGT GAACTGCAAG
    AGGGCACAGG CAGGAGCCAC AGATAATGAA AAAAGATCAG AGAACCAAGG GACAGCCCCA
    GCCTTCAAGG AGAAGCTGCA AGATGTCCAT GTGACAGAGG GTGAGAAGCT GCTACTCCAG
    TGTCAGGTGT CGTCTGACCC TCCAGCCACC ATCACCTGGA TGCTCAATGG AAAGACACTC
    AAGACCACCA AGTTCATCAT CCTCTCCCAA GAAGGCTCAC TGTGCTCCGT GTCCATCGAG
    AAGGCCCTGC CCGAGGACAG AGGCTTCTAC AAGTGCATAG CTAAGAATGG CGCCGGCCAG
    GCTGAGTGCT CCTGCCAAGT CACCGTGGAC GATGCTCCCA CCAAGGAGAA TGCCAAGGCC
    CCGGAGATGA AAGCCCGGAG GCCCAAGAGC TCTCTACCTC CCATGCTAGG AACAGAGAGT
    GATGCAACTG TGAAAAAGAA ACCTGCCCCC AAGACACCTC CAAAAGCAGC CATGCCCCCT
    CAGATCATCC AGTTTCCTGA GGACCAAAAG GTGCGCGCAG GAGAGTCTGT GGAGCTGTTT
    GGTAAAGTGG CAGGCACCCA GCCCATCACC TGTACGTGGA TGAAGTTCCG AAAGCAGATC
    CAGGAGAGCG AGCACATCAA GGTGGAGACC AGTGAGAATG GCAGCAGGCT CACCATCGTG
    GCCGCACGCC AGGAACACTG CGGCTGCTAC ACTCTGCTGG TGGAGAACAA GCTGGGCAGC
    AGGCAGGCCC AGGTCAACCT CACTGTCGTG GACAAACCAG ACCCCCCAGC GGGCACACCT
    TGCGCCTCCG ACATCCGGAG CTCGTCGTTG ACCCTGTCCT GGTATGGCTC CTCGTATGAT
    GGGGGCAGTG CCGTGCAATC CTACAGCGTC GAGATCTGGG ACTCGGTGGA CAAGACGTGG
    AAGGAACTAG CCACGTGCCG CAGCACCTCT TTTAACGTCC AGGACCTGCT GCCTGACCGA
    GAGTATAAAT TCCGCGTTCG CGCCATCAAC GTGTATGGAA CCAGTGAGCC AAGCCAGGAG
    TCTGAACTCA CAGCAGTGGG AGAGAAACCG GAGGAGCCGA AGGACGAAGT GGAGGTGTCG
    GATGATGATG AGAAGGAACC TGAGGTCGAC TACCGGACAG TGACGGTCAA CACTGAACAA
    AAAGTGTCCG ACTTCTATGA CATCGAAGAG AGACTAGGCT CTGGGAAATT TGGACAGGTC
    TTCCGACTTG TAGAAAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTCTT CAAGGCATAT
    TCAGCCAAAG AGAAGGAGAA TATCCGGCAG GAGATTAGCA TCATGAACTG CCTCCACCAC
    CCCAAGCTGG TCCAGTGTGT GGATGCCTTC GAAGAAAAGG CCAACATCGT CATGGTCCTG
    GAGATCGTGT CGGGGGGCGA GCTGTTCGAG CGCATCATCG ACGAGGACTT CGAGCTGACG
    GAGCGCGAGT GCATCCAGTA CATGAGGCAG ATCTCGGCGG GCGTGGAGTA CATCCACCGG
    CAGGGCATCG TCCACCTGGA CCTCAAGCCC GAGAACATCA TGTGCGTCAA TAAGACCGGC
    ACGAGGATCA AGCTCATCGA CTTCGGCCTG GCCAGAAAGC TGGAGAATGC GGGGTCTCTG
    AAGGTCCTCT TTGGTACCCC GGAATTTGTG GCCCCCGAAG TGATCAACTA TGAGCCCATC
    GGCTATGCCA CGGACATGTG GAGCATCGGA GTCATCTGCT ACATCCTGGT TAGTGGACTC
    TCCCCCTTCA TGGGTGACAA CGATAATGAA ACCCTAGCCA ACGTTACCTC AGCCACCTGG
    GACTTCGATG ATGAGGCGTT CGATGAGATC TCGGATGACG CCAAGGATTT TATCAGCAAT
    TTGCTGAAGA AAGATATGAA AAACCGCCTG GACTGTACCC AGTGCCTTCA GCATCCCTGG
    CTCATGAAAG ACACCAAGAA CATGGAAGCC AAGAAACTCT CCAAGGACCG GATGAAGAAG
    TACATGGCAA GAAGGAAATG GCAGAAAACG GGCAATGCTG TGAGAGCCAT CGGAAGACTG
    TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CCTCAACAGG ATCACCGACC
    AGCCCGCTCA ACGCAGAAAA ACTAGAATCT GAAGAAGATG TGTCCCAAGC TTTCCTTGAG
    GCTGTTGCTG AGGAAAAGCC CCATGTAAAA CCCTATTTCT CTAAGACCAT TCGTGATTTA
    GAAGTTGTGG AGGGAAGCGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCT
    GAAGTTGTCT GGTTTAAAGA TGACCAGTCA ATCCGGGAGT CCCGCCACTT CCAGATAGAC
    TACGACGAGG ATGGGAACTG CTCTTTAATC ATTAGCGATG TTTGTGGGGA TGACGATGCC
    AAGTACACCT GCAAGGCTGT CAATAGTCTT GGAGAAGCCA CTTGCACAGC AGAGCTCATT
    GTGGAGACCA TGGAGGAAGG GGAAGGGGAA GGGGAAGAGG AAGAAGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965710.1
    CDS79..6075
    Translation

    Target ORF information:

    RefSeq Version XM_014110235.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris myosin light chain kinase (MYLK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGGGGATG TGAAGCTGGC CGCCTTGACA CAGGCTTCTA AAACCTCCGT CAGTGTGGAT 
    CACTCGAGGG CCGGGTCTGC ACCCCCGACA GAGGCCCCTG CTTTCATTTT GCCCCCTCGG
    AACCTCTGCA TCAGAGAAGG AGCCACCGCC AAGTTTGAAG GGAGGGTCCG GGGTTACCCA
    GAGCCCCAGG TGACATGGCA CCGAAATGGG CAACCCATCA CCAGTGGGGG CCGCTTCCTG
    CTGGATTGTG GTATCCGGGG GACTTTCAGC CTCGTGATTC ATGCTGTCCG TGAGGAGGAC
    CAGGGAAAGT ATACCTGCGA AGCCACCAAT GATGGTGGTG CCCGCCAGGT GACAGTGGAG
    TTGACTGTAG AAGGGGGCTT CATGAAGAAG CATGGTCAGC CCGTTGTTTC CAAAACCTTG
    GGGGACAGAT TTGCAGCCCC CGCAGTGGAG ACCCGTCCTA GCATCTGGGG AGAGTGCCCA
    CCAAAGTTTG CTACCAAGTT GGGCCGAGCC GTAGTCAAAG AAGGACAGAC GGGACGGTTC
    TCCTGCAAGA TCACTGGACG GCCCCAACCA CAGGTCACCT GGCTCAAGGG GGATGTTGCC
    CTGCAGCCAA GTGCCCGTGT GTCTATGTCT GAGAAGAACG GGGTACAAGT TCTGGAAATC
    CATGAGGTCA GCCGCGATGA TGTGGGAGTG TACACATGCA TGGTGGTGAA TGGGTCGGGG
    AAGGCCTCCA TGTCGGCTGA GCTTTCTATC CAAGGTTTGG ACAATACCAG TAGGTCATTA
    ACAAAGGTGG CCAATTCAGA TATCAGGAAG GAGGTGACCA ATGGAACCAC GCAGGGGCCG
    AAACTAGACC ACCTGGAGTC TGCAGCGGAA AGGTGGCCCT GCTCCAGCAC CCAGATCGGT
    GGCTCCCCCA CCTGGGCCAC AAGTAGCCAG CCTCAGCCCC TGAGGGAGTC CAAGCTGGAG
    CCGCCTGGGG ACCCACCCAG GAAAGCCCTG AGGAGCCCCG TCCTACAGAA GACTTCCAGC
    ACCATTACCC TGCAGGCGGC AAAAGTCCAG CCAGAGCCAA GAGCGCCGGT CTCAAGTGCC
    CTCTCGTCTC CCGGAGAAGA GAGGGAGAGG CCAGCTGCTC CCCCCACAGC CACCTTCCCT
    GCCCGGCAGT CTGGCCTGGG AAGCCAGGAG GTTGTGAACA AGGTTGCTAC CAGAAAAATC
    CCCATGGAAA GCCAGAGAGA TTCCACATTC CCCAAATTTG AGAGCAAGCC CCAAAGCCAG
    GAGGTCAGTG AAGATGAAAC AGTCAAGTTC AGATGTGAGG TTTCAGGGAC CCCAAAGCCT
    GAAGTGACCT GGTTCCTGGA AGGTGCCCCC GTGAAGAGAC GAGAAGGCAT GGTTGAGGTT
    TATGAAGAAG GTGGATGCCA CTACCTCTGC TTGCTGAGAG TGCGGGCCAA GGACAGTGGG
    AGCTACAGTT GCACGGCCTC CAATGTCCGA GGCCAGGTGT CCTGTGGTTG GACCCTCCTG
    GTGAAACGGC TGGCCGTGAT GGAGGTGGCT CCCTCGTTCT CCAGCGTCCT GAAGGACTGC
    GCTGTCATTG AGGGCCAGGA CTTTGTGCTG CAGTGCTCAG TGCAGGGGAC CCCGGTGCCC
    CAAATCACTT GGCTGCTTAA TGAGCAGCCC ATCCAGTACG CTCACTCCTC CTGCGAGGCT
    GGCGTGGCCG AGCTCCACAT CCAGGATGCC CTGCCGGAAG ATGATGGCAT TTACACCTGT
    CTGGCCAAGA ACACCTTGGG CCAGGTGTCC TGCAGCGCCC GGGTCACCGT CCATGAAAAG
    AAGAGCGACA GGAAGAGTGG GTATCTTCTG CGCACAGCTC CCGGCAAGCC CGTCGCACCC
    CTCTTCCTGC AGGGCCTCTC TGACCTCAAA GTCATGGATG GAAGCCAGGT CACCATGACA
    GTCCAGGTGT CAGGGAATCC GCCCCCCGAG GTCATCTGGC TTCACAACGG GAATGAGATC
    CAGGAGTCAG AGGACTTCCA CTTTGAACAG AGAGGCACTC AGCACAGCCT TTGTATCCAG
    GAAGTGTTCC CAGAGGACAC AGGCACTTAC ACCTGCGAAG CCTGGAACAG TGCTGGAGAG
    GTCCGCACCC AGGCAGTACT GATGGTGCAA GAGCCCCAGG ATGGCACCCA GCCCTGGTTC
    ATCAGCAAGC CCCGCTCACT GACTGCCTCC CTGGGCCAGA CTGTCCTCAT CTCCTGTGCC
    ATCGCTGGGG ACCCCTTCCC CACTGTGCAC TGGCTGCGAG ATGGCAAAGC CCTCTCCAAA
    GACTCCGGCC ACTTCGAGGT GCTACAGAAC GAGGACGTGT TCACCCTGGT TCTAAAGAAT
    GTGCAGCCCT GGCACGCCGG CCAGTACGAG ATCCAGCTCA AGAACCGGGT TGGCGAATGC
    AGTTGCCAGG TGTCACTGAT GCTACAGAGC AGCCCCACCG GAGCCCCCCT GCGGGGGAGG
    GAGCCTGCCA GCTGCGAGGG CCTCTGCAGC CGGGGAGCCG GCGCTGATGG TGGTGGCGGT
    GACTGCTATG GGACCCTGAG GCCTGGCCGG CCAGCGAGAG GACAGGGTTG GCCAGAGGAG
    GAGGACGACG GCGAGGACGT GCGGGGGGTG CTGAAGAGGC GCGTGGAGAC CCGGCAACAT
    ACTGAGGAGG CCATCCGCCA GCAGCAGGTG GAACAGCTGG ACTTCCGTGA CCTCCTGGGG
    AAGAAGGTGA GTACCAAGAC CGTGTCAGAA GAAGACCTGA AGGAGATCCC AGCGGAGCAG
    ATGGATTTCC GCGCCAACCT GCAGCGGCAA GTGAAGCCAA AGACTGTGTC AGAGGAAGAG
    AGGAAGGTGC ATAGCCCTCA GCAGGTCGAC TTCCGCTCGG TCCTGGCCAA AAAGGGGACT
    CCTAAGACCC CGGTGCCTGA GAAGGCACCT CTTCCCAAAC CCGCCACCCC AGATTTCCGC
    TCCGTGTTAG GTAGCAAGAA AAAATTACCA GCCGAGAATG GTAGCAACAA CGCTGAGGCC
    TTGAATGCCA AGGCAGCAGA AAGTCCCAAG GCTGTGAGCA ATGCCCAGCC TTTAGGGTCC
    CTGAAACCTT TGGGCAATGC CAAGCCTGCT GAGACTCTAA AACCTGTGGG CAACATCAGG
    CCTGCTGAGA CGCTGAAACC CATAGGCAAC GCCAAACCTG CTGAGACCCT GAAACCCATG
    GGCAACGCCA AGCCTGCTGA GACGCTAAAA CCCATAGGCA ATGCCAAGCT TGCTGAGACC
    CCGAAACCCA TGGGCAATGC CAAGCCTGCT GAGACCCTGA AACCCCTGGG CAACGCCAAG
    CCTGCTGAGA CGCTGAAACC CATAAGCAAC GCCAAGCCTG CTGAACCTCT GAAACCTGTG
    GGCAACGCCA AGCCTGCTGA GACCCCAAAA CCCATAGGTA ATGCCAAGCC TGCTGAGATC
    CTAAAACCAG CTGGGAAGGA AGAACTCAAG AAGGAGGTTA AGAATGACGT GAACTGCAAG
    AGGGCACAGG CAGGAGCCAC AGATAATGAA AAAAGATCAG AGAACCAAGG GACAGCCCCA
    GCCTTCAAGG AGAAGCTGCA AGATGTCCAT GTGACAGAGG GTGAGAAGCT GCTACTCCAG
    TGTCAGGTGT CGTCTGACCC TCCAGCCACC ATCACCTGGA TGCTCAATGG AAAGACACTC
    AAGACCACCA AGTTCATCAT CCTCTCCCAA GAAGGCTCAC TGTGCTCCGT GTCCATCGAG
    AAGGCCCTGC CCGAGGACAG AGGCTTCTAC AAGTGCATAG CTAAGAATGG CGCCGGCCAG
    GCTGAGTGCT CCTGCCAAGT CACCGTGGAC GATGCTCCCA CCAAGGAGAA TGCCAAGGCC
    CCGGAGATGA AAGCCCGGAG GCCCAAGAGC TCTCTACCTC CCATGCTAGG AACAGAGAGT
    GATGCAACTG TGAAAAAGAA ACCTGCCCCC AAGACACCTC CAAAAGCAGC CATGCCCCCT
    CAGATCATCC AGTTTCCTGA GGACCAAAAG GTGCGCGCAG GAGAGTCTGT GGAGCTGTTT
    GGTAAAGTGG CAGGCACCCA GCCCATCACC TGTACGTGGA TGAAGTTCCG AAAGCAGATC
    CAGGAGAGCG AGCACATCAA GGTGGAGACC AGTGAGAATG GCAGCAGGCT CACCATCGTG
    GCCGCACGCC AGGAACACTG CGGCTGCTAC ACTCTGCTGG TGGAGAACAA GCTGGGCAGC
    AGGCAGGCCC AGGTCAACCT CACTGTCGTG GACAAACCAG ACCCCCCAGC GGGCACACCT
    TGCGCCTCCG ACATCCGGAG CTCGTCGTTG ACCCTGTCCT GGTATGGCTC CTCGTATGAT
    GGGGGCAGTG CCGTGCAATC CTACAGCGTC GAGATCTGGG ACTCGGTGGA CAAGACGTGG
    AAGGAACTAG CCACGTGCCG CAGCACCTCT TTTAACGTCC AGGACCTGCT GCCTGACCGA
    GAGTATAAAT TCCGCGTTCG CGCCATCAAC GTGTATGGAA CCAGTGAGCC AAGCCAGGAG
    TCTGAACTCA CAGCAGTGGG AGAGAAACCG GAGGAGCCGA AGGACGAAGT GGAGGTGTCG
    GATGATGATG AGAAGGAACC TGAGGTCGAC TACCGGACAG TGACGGTCAA CACTGAACAA
    AAAGTGTCCG ACTTCTATGA CATCGAAGAG AGACTAGGCT CTGGGAAATT TGGACAGGTC
    TTCCGACTTG TAGAAAAGAA AACTGGAAAA ATCTGGGCAG GGAAATTCTT CAAGGCATAT
    TCAGCCAAAG AGAAGGAGAA TATCCGGCAG GAGATTAGCA TCATGAACTG CCTCCACCAC
    CCCAAGCTGG TCCAGTGTGT GGATGCCTTC GAAGAAAAGG CCAACATCGT CATGGTCCTG
    GAGATCGTGT CGGGGGGCGA GCTGTTCGAG CGCATCATCG ACGAGGACTT CGAGCTGACG
    GAGCGCGAGT GCATCCAGTA CATGAGGCAG ATCTCGGCGG GCGTGGAGTA CATCCACCGG
    CAGGGCATCG TCCACCTGGA CCTCAAGCCC GAGAACATCA TGTGCGTCAA TAAGACCGGC
    ACGAGGATCA AGCTCATCGA CTTCGGCCTG GCCAGAAAGC TGGAGAATGC GGGGTCTCTG
    AAGGTCCTCT TTGGTACCCC GGAATTTGTG GCCCCCGAAG TGATCAACTA TGAGCCCATC
    GGCTATGCCA CGGACATGTG GAGCATCGGA GTCATCTGCT ACATCCTGGT TAGTGGACTC
    TCCCCCTTCA TGGGTGACAA CGATAATGAA ACCCTAGCCA ACGTTACCTC AGCCACCTGG
    GACTTCGATG ATGAGGCGTT CGATGAGATC TCGGATGACG CCAAGGATTT TATCAGCAAT
    TTGCTGAAGA AAGATATGAA AAACCGCCTG GACTGTACCC AGTGCCTTCA GCATCCCTGG
    CTCATGAAAG ACACCAAGAA CATGGAAGCC AAGAAACTCT CCAAGGACCG GATGAAGAAG
    TACATGGCAA GAAGGAAATG GCAGAAAACG GGCAATGCTG TGAGAGCCAT CGGAAGACTG
    TCCTCTATGG CAATGATCTC AGGGCTCAGT GGCAGGAAAT CCTCAACAGG ATCACCGACC
    AGCCCGCTCA ACGCAGAAAA ACTAGAATCT GAAGAAGATG TGTCCCAAGC TTTCCTTGAG
    GCTGTTGCTG AGGAAAAGCC CCATGTAAAA CCCTATTTCT CTAAGACCAT TCGTGATTTA
    GAAGTTGTGG AGGGAAGCGC TGCTAGATTT GACTGCAAGA TTGAAGGATA CCCAGACCCT
    GAAGTTGTCT GGTTTAAAGA TGACCAGTCA ATCCGGGAGT CCCGCCACTT CCAGATAGAC
    TACGACGAGG ATGGGAACTG CTCTTTAATC ATTAGCGATG TTTGTGGGGA TGACGATGCC
    AAGTACACCT GCAAGGCTGT CAATAGTCTT GGAGAAGCCA CTTGCACAGC AGAGCTCATT
    GTGGAGACCA TGGAGGAAGG GGAAGGGGAA GGGGAAGAGG AAGAAGAGGA AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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