PKD1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PKD1
Entrez Gene ID	606755
Full Name	polycystin 1, transient receptor potential channel interacting
Synonyms	PC1
General protein information	Preferred Names polycystin 1, transient receptor potential channel interacting Names polycystin-1 polycystic kidney disease protein 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001006650.1	NP_001006651.1	polycystin-1 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PKD1	XP_003587846.1
Canis lupus familiaris (dog)	PKD1	NP_001006651.1
Homo sapiens (human)	PKD1	NP_001009944.2
Gallus gallus (chicken)	PKD1	XP_414854.4
Xenopus tropicalis (tropical clawed frog)	pkd1	XP_002932517.2
Mus musculus (house mouse)	Pkd1	NP_038658.2
Rattus norvegicus (Norway rat)	Pkd1	NP_001244281.1
Danio rerio (zebrafish)	LOC100149562	XP_003197787.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide methylation profiling of ADPKD identified epigenetically regulated genes associated with renal cyst development.
Woo YM, Bae JB, Oh YH, Lee YG, Lee MJ, Park EY, Choi JK, Lee S, Shin Y, Lyu J, Jung HY, Lee YS, Hwang YH, Kim YJ, Park JH
Human genetics133(3)281-97(2014 Mar)

Hyperphosphorylation of polycystin-2 at a critical residue in disease reveals an essential role for polycystin-1-regulated dephosphorylation.
Streets AJ, Wessely O, Peters DJ, Ong AC
Human molecular genetics22(10)1924-39(2013 May)

A non-synonymous mutation in the canine Pkd1 gene is associated with autosomal dominant polycystic kidney disease in Bull Terriers.
Gharahkhani P, O'Leary CA, Kyaw-Tanner M, Sturm RA, Duffy DL
PloS one6(7)e22455(2011)

Analysis of the cytoplasmic interaction between polycystin-1 and polycystin-2.
Casuscelli J, Schmidt S, DeGray B, Petri ET, Celi? A, Folta-Stogniew E, Ehrlich BE, Boggon TJ
American journal of physiology. Renal physiology297(5)F1310-5(2009 Nov)

Linkage confirms canine pkd1 orthologue as a candidate for bull terrier polycystic kidney disease.
O'Leary CA, Duffy D, Biros I, Corley S
Animal genetics40(4)543-6(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

treatment with DNA methylation inhibitors retarded cyst formation of Madin-Darby Canine Kidney cells, accompanied with the upregulation of Pkd1 expression.
Title: Genome-wide methylation profiling of ADPKD identified epigenetically regulated genes associated with renal cyst development.

These results reveal a reciprocal functional link between PC1 and PC2 which is critically dependent on their interaction.
Title: Hyperphosphorylation of polycystin-2 at a critical residue in disease reveals an essential role for polycystin-1-regulated dephosphorylation.

Bull terriers polycystic kidney disease is associated with a missense mutation in Pkd1.
Title: A non-synonymous mutation in the canine Pkd1 gene is associated with autosomal dominant polycystic kidney disease in Bull Terriers.

PC1 C-terminal tail in MDCK cells alters the Ca2+ response, but that overexpression of the PC1 C-terminal tail containing the disease mutation does not
Title: Analysis of the cytoplasmic interaction between polycystin-1 and polycystin-2.

Pkd1 is associated with polycystic kidney disease in bull terriers.
Title: Linkage confirms canine pkd1 orthologue as a candidate for bull terrier polycystic kidney disease.

The following PKD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00340	NM_001006650.1 Latest version!	Canis lupus familiaris polycystin 1, transient receptor potential channel interacting (PKD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PKD1 ( NM_001006650.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001006651.1 Canis lupus familiaris polycystin 1, transient receptor potential channel interacting (PKD1), mRNA.

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CloneID	OCe00340
Clone ID Related Accession (Same CDS sequence)	NM_001006650.1
Accession Version	NM_001006650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-21
Organism	Canis lupus familiaris(dog)
Product	polycystin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY102170.1. On Jan 18, 2005 this sequence version replaced XM_537007.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY102170.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGCCGCCCG CCGCGCCCGG CCGCCTGGCG CTCGCCCTGG GCCTGGGCCT GTGGCTCGGG 
GCGCTGGCGG GGGGCCCCGG GCGCGGCTGC GGCCCGTGCG CGCCCCCCTG CCTGTGCGGC 
CCTGCGCCCG GCGCCGCCTG CCGCGTCAAC TGCTCGGGCG GCGGACTGCG GACGCTCGGA 
CCCGCGCTGC GCATCCCCGC CGCCGCCACC GCGCTAGACG TCTCCCACAA CCTGCTCCGG 
GCATTGGACG TTGGGCTCCT GGCAAACCTC TCTGCACTGA CAGAGCTGGA TATCAGTAAC 
AACAAGATTT CAACTCTAGA AGAAGGAATA TTTTCTAACT TATTTAACTT AAGTGAAATA 
AACCTGAGCG GAAACCCGTT GGAGTGTAAC TGCGGTCTGG CGTGGCTGCC TCGCTGGGCG 
AAAGAGCAGC AGGTTCACGT GGTTCGGCCT GAGGCGGCCA CGTGTGCTGG GCCCGGCCCC 
CTAGCTGGCC AGCCCCTCCT CGGGGTCCCC TTGTTGGATG GTGGCTGTGG TGAGGAGTAC 
ATTGCCTGCC TCCCTGACAA CAGCTCGGGT GCTGTGGCGC TGGTGGCCTT CTCAGCCGCC 
CACGAGGGCC CACTGACGCC CGAGGCCTGC GGTGCCTTCT GTTTTGCGGC TGGCAATGGC 
CTTGGGGCCT TCTCAGACCA GGGCTGGTGC CTGTGCGGGG CCGCCCAGCC CCCCAACACC 
TCCTCAGCCT GCCTGCCCTT CTGCTCCGGC GCCCCACTGG CCCTTGCCCC TGCGTGTGGG 
GGCCCCACTC TCCTCCAGAA CATCTTCCCC GCCTCCCCAG GGGCCGCCCT GGTGGGGCCT 
CAGGGACCCC TGGCCTCTGG CCAGCCAGCA GCCTTCCATG TCACTGCCTC CCTGCCCGTC 
AGCTCCACAC GCTGGGACTT CGGTGATGGC TCCCCCAAGG TGGACATTGC TGGTCCTGCC 
ACCACTCACC GCTACCTGCT GCCCGGGCTC TATCACGTGA CTGTTGTGCT GGCCCTGGGG 
GCTGGCTCAG CCCAGGTAGA GACCCAGGTG CACGTGGAGG CGGCCCCTGC CTCCCTGGAG 
CTCACGTGCC TCACCTCGGT GCGCAGCGAT GAGACCCTCA AGTTTGGCAT CCGAAATCGT 
GGCGGCTCGG ACCTGGAAGC CACATACAGC ATTGTGGCTG TGGGCGAGGA GCCAGCCCAA 
GACCCCGCAG TGGTGCATCC GCTGTGCCCC TCGGACACCG TCATCTTCCC TGGTAATGGG 
CACTGCTACC GTCTGGTGGC AGAGAAGGCT GCCTGGCTGC AGGCGCAGGA GCAGTGCCGG 
GCCTGGGCTG GGGCCGCCCT GGCCATGGTG GACAGTCCCG CCATCCAGCA CTTCCTGGTC 
TCCCAGGTCA CCAGGAGCCT GGACGTGTGG ATCGGCTTCT CAGCCGTGGA GGGGGCGGAG 
GCGGGCCCGG TGGTGCAGGG TGCCGCCTTC AGCCTCGAGA GCTGCCAGAA CTGGCTCCCC 
GGGGAGCCGC ACCCCGCCAC CGCCGAGCGC TGCGTGCGGC TCGGGCCTGC GGGCCAGTGC 
AACACGGACC TGTGCTCGGC GCCCCATAGC TACGTCTGCG AGCTGCGGCC TGGAGGTCCC 
GTGTGGGACG CGGAGAACTT CCTCGTGGGA GCGCCCATTG GGGACCTGCA GGGACCCTTG 
AGCCCCCTGG CACAGCAGGA GGCCCTCTCG GCCCCCCAGG AGCTTATCGA GGTGATGGTG 
TTCCCCAGCC TGGGCCTGAG CAGGGAAGCC TTCCTCACTG CAGCTGAGTT TGGCACCCAG 
GAGCTGCGCC GGCCCACCCA GCTGCGACTG CAGGTGTTTC GGCCCGTGGG AGAAGCAGGG 
GCCCCAGGGA ACAGCAGGGA GCCTGAGAGT GGGTCCCGGG AGAGCCGGAC TGAGTCAGTC 
CCTGAGTGCA CCCCAGAGGG ACGCTGGTGC CCCAGAGCCA GTATCCACCT GCCACCAGAT 
GACCCCTGCG GCCCCTGGGC CTGTGCCAAT GGGTCCATGT CTGGGCCAGG ACTCCCCGGG 
GCCTCCTGTG TGCTGCTGAG GGAGTTCCTC TTCTCTGTAC CGGCGGGTCC TCCTTCCCAG 
TACTCAGTCA CCTTCCGTGG CCAGGACGGG CCCCTGAAGC CTGGTGACCT CATCGCCCTG 
CAGCATGACG CGGGACCAGG TGCCCTTCTG TGCTGCTCTC CAGCACTTGG TGCCCCTGGG 
GCTGAGGCCC CCTACTTTTC CGCCACCTCC CCGGCCTGGC TGGTGCACCT CCCTGCTCAG 
CTGGAGGCAG CCCCGGCTGG CCCAGCCTGT GCCTTGCGGC TGCTTGTGGC TACAGAGCGC 
CTCACCACCC TCCTGGGCCT GCGGCCCAAC CCAGGGCTGC GGCGGCCTGG GCGCTATGAG 
GTCCGGGCCA CCGTGGGCAA CAGCGTGTCC ACACACAACC TGTCCTGCAG CTTTGACGTG 
CTCTCCCCCG TAGCTGGGTT GCGTGTCGCC CACCCGATCC CCCACGACGG CCGCCTCTAC 
GTGCCCACCA ACAGCTCAGT CTTGGTGCTC CAGGTGGACT CTGGCACCAA CGCCACCGCC 
ACGGCCCGCT GGCCTGGGGG CAATGCCAGT GCACCCTTTG AAGCCACCTG CCCTGCCACA 
GTGGCTGCCC TGGTACCCAG CTGCACTCTT GAGGCCAATG ACACCCTGTT TGCGGTGCTT 
GTGCTGCCTG GGCTCAGCGA GGGTGAGCAC GCGGTGGAGA TCATGGTGGA GAACAGCGCC 
GGCCACGCCA ACCTCAGCCT GCGGGTCAAG GCCGAGGAGC CCATCTGTGG CCTTCGTGCC 
GCCCCCAGCC CCGAAGCCCG TGTGCTGCAG GGAGTCCTGG TGAGGTACAG CCCCGTGGTG 
GAGGCCGGCT CTGATGTGGT CTTCCGCTGG ACCATTGATG ACAAGCAGTC CCTGACCTTC 
CACAACGTGG TCTTCAACGT CATCTACCAA AGTGCTGCCG TCTTCAAGCT CTCGCTGACG 
GCCTCCAACC ACGTGAGCAA CGTCACCGTA AATTACAATG TCACCGTGGA GCGGATGCAC 
AAGATGAAGG GCCTTCGGGT GTCTGCCGTG CCACCCGTGT TGCCTCCCAA CACGACTCTG 
GAGCTGGCCG CGCACGTGCT GGTGGACTCA GCTGTGGAGG TGGCCTTTCT GTGGACCTTT 
GGGGATGGGG ATCAGGTGCT CGGCCAGTTC AAGCCTCCAT ACAATGAGTC CTGGGTCCCA 
GACCCCACGG TGGCCCAGGT GCTGGTGGAG CACAGTGTCA CACACGTCTA TGCTGTCCCA 
GGTGAGTACA ACCTGACCCT GCTGGTGTCC AACGCCTTTG AGAACCTGAC GCAGCAAGTG 
CCTGTGAGTG TGCGCGCCAC CTTACCTGCG GTGGCTGTGG CTGTGGGAAG CCACGTCCTG 
GTGGCTGGGC AGCCTGTCAC CTTCTCCCCA CACCCACTGC CTTCACCTGG GGGCATTTTG 
TACACGTGGG ACTTTGGGGA TGGCTCCCCA GCTGTGACGC AGCACCAAAC AGAGGTCAGC 
CACACATATG CCTCGAGGGG TATTTACCGC ATCCATCTGG AGGTGAACAA CACGGTTAGC 
AGTGTGACAG CGTGGACCAG CGTCCGCGTC TTTGAGGAGC TCCGAGGCCT GAGTGTGAGC 
CTGAGCCCAT CTGTGGAACA GGGTGCGCCC GTGACGGTCA GCGCCACCCT GGAGTCGGGA 
GACAACGTCA CGTGGACTTT TGACATGGGG GATGGCACCG TGCTTGTGGG CCCCGAGGCC 
ATGGTGGAAC ACGTTTACCT GCGGGCACAG AACCACACGG TGACCGTGGG CGTGTCCAGC 
CCTGCGGGCT ACCTGGCCCT GAGCCTGCCT GTGCACGTCT TCGTCCTGGA GGTGCTGCGG 
ATTGAGCCTG CGGCCTGCAT CCCTGTGCAG CCCCACACCT GGTTCATGGC CCACGTCACC 
GGGGACCCTG CCCACTACAT CTTTGACTGG ACGTTCGGGG ATGGGTCATC CAACACGACC 
ATCTTTGGGG ACCCAACGGT GACACACAAT TTCACACGGA GCGGAACGTT CCCCTTGGCG 
TTGGTCCTAT CAAGCCATGT GAACAAGGCC CATTACTTCA CCAGCGTCTG CGTGGAGCCC 
GAGGTGGGCA ACGTCACCCT GTGGCCCGAG AGGCAGTTTG TGCGCCTCGG GGATGAGGCC 
CATCTGGTGG CTCGCGCCTG GCCCCCCTTC CCCTACCGTT ATACCTGGGA CTTTGGCCCT 
GAAGACGCGC CCCGTGTTGG GGGCCCCGAG GCCACGTTCA CCTATGCGGA CCCAGGTTCC 
TACCTGGTGA CGGTCGCTGT CTCCAACAAC ATCTCGGCCG CCAATGACTC TGCACTCGTG 
GAAGTGGAGG AGCCCGTGGA GCTCACGGGC ATTGGGGTCA ACGGCTCTGG TGTGCTCGAG 
CTGCAGCGGC CCTACCTGTT CTCTGCTGTG GGCCGCGGAC ACCCCGCCAC CTACCTGTGG 
GAACTGGGGG ACGGCGGGCG CCTCGAGGGC CCCTCCATCA AGCACACCTA CAACAGCACA 
GGCCACTTCA CCGTCAGGGT GAGCGCGTGG AATGAGGTGA GCCGCGGCGA GGCCTGGTTG 
AACATCACGG TGCAGCGGCG CGTGCAGGGG CTCAGCGTCA ACGCCAGCCG CACGGTGGTG 
CCCCTCAACG GCAGCGTGAG CTTCACCACC TCCCTGGAGG CCGGCAGCGA CGTGCGCTAC 
TCCTGGGTGC TCTGTGACCG CTGCACGCCC ATCCCTGGGG GCCCCACCAT CTCCTACACC 
TTCCGCTCCG TGGGCACCTT CAACATCATC GTGACCGCTG AGAACGAGGT GGGCTCTGCC 
CAGGACAGCA TCTTCATCTA CGTGCTGCGG CACATCGAGG GGCTGCAGGT GGCAGGGGGT 
GGGGGCGGCT GCTGCTTCCC CACCAACTGC ACGCTGCAGC TGCAGGCCGT GGTCAGCGAC 
GGCACCAACA TCTTCTACAG CTGGAGCGCC CAGAGGGATG GCGGCCCGGC CCTGGCTGGC 
AGCGGCAAAG CCTTCTCGCT CACCGTGCTC GAGGCCGGCA CATACCACGT CCAACTGCGG 
GCTGCCAACA TGCTGGGCAC GTCCTGGGCC AACCGCACGG TGGACTTCGT GGAGCCCGTG 
GGCGCACTGG CTGCCGCCGC CTCCCCAAAC CCGGCCATAG TCAATTCCAG CGTCACCCTC 
TGTGCCGAGC TGGCTGGGGG TAGTGGTGTC ACTTACACCT GGTCCCCGGA GGGAGGACTG 
AGCTGGGAGA CCCCAGAGCC ATCCTCCACT CACGTCTTCC CCAGCCCCGG CCTGTACCTG 
GTCAGGGTGA CAGCTACGAA CTGGCTGGGC TCAGCCAATG TGACCATCGA GGTGGCTGTG 
CAGGTGCCGG TCAGTGGCCT CAGCATCCGG GCCGGCGACA TAGACCGCGG CTTCGTGGCA 
GCAGGCTCTG CGGTGCCCTT CTGGGGACAG CTGGACTCAG GCACCAACGT GAGCTGGTGC 
TGGACGGTGC CAGGGGGCAG CAGGCGTGGC CAGCACGTTG CTGTGGTCTT CCGTGACGCA 
GGCGCCTTCT CCGTGCGGCT CAATGCCTCC AATGCAGTCA GCTGGGTCAC GGCCACCCGT 
GAGCTCACGG TGGAGGAGCC CATTGCGGGC CTGGTGGTGT GGGCCGACAG CAAGGTGGTG 
GAGCCGGGGC AGCTGGTCCA CTTCCAGGTC ATGCTGGCTG CCGGCTCCGC TGTGCGCTTC 
CATCTGCAGG TCAGCGGGGC CGCTGTGGAG GCGCTCCCCG GGCCCCGCTT CTCCCGCAGC 
TTCCCCCGAG TTGGGGACTA CATGGTGAGC GTCCAGGCTG AGAACCACGT GAGCCGGGCC 
CAGGCCCAGG TGCGCATCTT GGTGCTCGAG GCTGTGGGTG GGCTCCAGGT GCCCCACTGT 
TGCGAGCTGG GCATCCCCAC GGGCACGGAG AGGAATTTTA CAGCCCGGGT GCAGCGTGGG 
TCGCGCGTGG CCTACGCCTG GTACTTCTCC TTGCAAAAGG TCCAGGGGGA CTCACTGGTC 
ATCCTTTCGG GCCGTGATGT CACCTACACC CCCGTGGCCG CGGGGCTGCT GGAAATCCAC 
GTGCGTGCCT TCAATGATCT GGGCGGCGTG AACCTCACGC TGCTGATGGA GGTCCAGGAT 
GCCATCCAGC ATGTGGTGCT GCGCAGTGGC CGCTGCTTTA CCAACCGCAC AGCCCGGTTT 
GAGGCCGCCA CAAGCCCTAG TCCCCGGCGT GTGGCCTATC GCTGGGACTT TGGGGACGGG 
GCCGTGGTGG AGGACACGGA GGAGCCCTGG GCTGAACACT CCTATCTGCG GCCGGGGGAC 
TACCGCATAG AGGTGAATGC CTCCAACCTC GTGAGCTTCT TCGTGGCCCA GGCCACGGTC 
ACTGTCCACG TGCTGGCCTG CAGGGAGCCC GAGGTGGACG TGGCCCTGCC CCCACAGGTG 
TTGATGCGGC GCTCCCAGCG CAACTACCTG GAGGCCCACG TCAACCTGCG CGACTGTGTC 
ACCTACCAGA CCGAGTACCG CTGGCAGGTG TACCGTGCTG CCAGCTGCCA GCGGCCTGGG 
CGCCTGGCCC CTGTGGCCCT GCCCAGTGTG GACATGAGCC GGCCCCAGCT GGTTGTGCCA 
CGGCTGGCAC TGCCTGTGGG CCACTACTGC TTTGTGTTCA TGGTATCATT TGGAGACACA 
CCGCTGGCAC GGAGCATCCA AGCCAATGTG ACTGTGGTGC CTGAGCGCCT GGTGCCCATT 
GTTGAGGGTG GCTCATACCG CGTGTGGTCA GACACTCAGG ATCTGGTGCT GGATGGGAGC 
AAATCCTATG ATCCCAACCT AGAGGATGGT GACCAGACAC CACTCAGCTT CCATTGGGCC 
TGTGTGGCCT CGACACAGAG TGAGACTGGT GGCTGTACCC TGACCTTCGG GCCCCGGGGA 
AGCAGCGTGG TCATCGTTCC TCGGGAGCGC CTGCAGGCTG GCGTGGAGTA CACCTTCAAC 
CTGACTGTGT GGAAGGCCGG CCGGAAGGAG GAGGCCACCA ACCAGACGGT GTTGATCCGC 
AGGGGCCGCG TGCCCATCGT GTCCTTGGAG TGCGTGTCCT GCAAGGCGCA GTCGGTGTAC 
GCAGTGAGTC GAAGCTCCTA CGTGTACCTG GAGGGCCGCT GTGATAACTG CAGCGAGGGT 
TCCAAGCGAG GGCGCTGGGC GGCGCGCACC TTCAGCAACC AGACGCTGGT GCTGGACGAG 
ACGACCACGT CGACGGGCAG CTCGGGCATG CGGCTGGTGC TGCGGCGCGG CGTGCTGCGG 
GACGGCGAGG GCTACACGTT CACACTCACC GTGCTGGGCC GCTCGGGCGA GGAGGAGGGA 
TGCGCCTCCA TCCGCCTGTC CGCCAACCGG CCCCCGCGCG GGGGCTCCTG CCGCCTCTTC 
CCGCTTGACG CCGTTCGCGC CCTCACCACC AAGGTGCATT TCGAGTGCAC AGGCTGGCAG 
GACGCCGAAG ACGCGAGCGC CCCACTGGTG TACGCACTGC TGCTGCGGCG CTGTCGCCAG 
GGCCACTGCG AGGAGTTCTG CGTGTACAGG GGCAGCCTCT CTGCCTACGG AGCTGTGCTG 
CCGCCCGGTT TCGCACCGCA CTTCCAGGTG GGCCTGGCCG TGGTCGTGCA GGACCAGCTG 
GGCGCAGCTG TGGTCGCTCT CAACAGGTCT CTGGCCATCA CCCTGCCACA GCCTCCTGGC 
GACCCCCAGG GAGGACCCAC AGACCTCACC AGCTGGTTGC ACGGCCTCAC GGAGAGCATG 
CTGCCCAGGC TGCTGAGACA GGCCGACCCC CAGCACGTCA TCGAGTACTC GCTGGCCCTC 
ATCACTGTGC TCAATGAGGC CAGTGCAGGG TCCGAGCAGG TTCTGGCCGT GGTGGCAGAG 
GAGGATGCCA GGCGGCAGCT GCAAGCCCAG ATCCGCAAGA ATGTCACAGA GACTTTGGTC 
TCCCTGCGGG TCAACACCGT GGATGACATC CAGCAGATCG CGGCAGCGCT GGCCCAGTGC 
ACGGTGTCCA GCAGGGAGCT CGTGTGCCAC TCCTGCCTGA AGAAGACGCT GCATAAGCTT 
GAGGCCATGA TGCGCATCCT GCAGGCTGAG ACGGCTGCTG GCACTGGGAC GCCCACGGGC 
ATAGCTGACA GCATTCTCAA CATCACAGGC GACCTCATCC ACTTGGCCAG TGTGGACATG 
CAGGGCCTGC AGCCCTCGGA GCTGGGCACA GAGCCGCCCT CACTCATGGT AGCATCCAGG 
GCCTACCACC TCTCATCGGC CCTCATGTGC ATTCTCATGC GCTCCCGTGT GCTCAACGAG 
GAGCCCCTGA CGCTGGCGGG TGAGGAGATT GTGGCCCAGG GCAAGCGCTC CGACCCGCTG 
AGCCTGCTGT GCCAGGGCAA CGCCTCGGTT CCCAGCTGCC ACTTCTCTAT CCCCACGGCC 
TTCAGCGGGG CCCTGTCCAA CCTCAGTGAT GTGGTGCAGC TCATGTTCCT CGTCGACTCC 
AATCCATTCC CCTTTGGCTA CATCAGCAAC TACACCGTCT CCACCAAGGT GGCCTCCATG 
GCCTTCCAGA CGCAGGCCGG CACCCAGATC CCCATCGGGC AGCTGGCCTC AGAGCGCGCC 
ATCATCGTGA AAGTGCCCAA CAGCTCCGAC CAGGCTGCCC CGGGCCACCG CGCCCCTGCT 
GGCTCCGCAG TCATCCAGCC CCAGGCCTCC ATCAGCGTTG CGGTCACCCC TGAGAACAGC 
AACCCGGCGG CCGGGCTGCA CCTGCAGCTC ACCTACACGC TGCTCAGTGA GCGCTACTTG 
CCCGAGGAGC CTGAGCCCTA CCTGGCTGTG TACTTTGACT CGGTGCCTGG GCCCAACGAG 
CACAACTGCT CCGCCAGCAG GAGGATCAGC CTGGAGGAGC TGGCGGGCGG CGACCACAGG 
CCCTATACCT TCTTCATTGC CCCAGGGACC AGAGACTCAG GTGGGACTTA TTACCTGAAT 
CTGACCAGCC GCTTCCACTG GTCAGCACTG GAAGTGAGCG TGGGCCTGTA CACGTCCCTG 
TGTCAGTACT TCAGTGAGAA GGAGATGATG TGGAAGACAG AGGGGCTTGT GCCTCTGGAG 
GAGACGTCCC CCAGCCAGGC CGTCTGCCTT ACCCGCCACC TCACTGCCTT TGGTGCCAGC 
CTCTTTGTCC CCCCCAGCCA CGTCCACTTC ATCTTTCCAG AGCCGGCCTC AGGCGTGAAC 
TACATCGTCC TGCTGACGTG TATCGTGTGC CTGGTCACCT ACACAGTCAT GGCCGTGATC 
CTGCGTAAGC TGGACCAGCT GGACGTCAGC CGGGTGCGCA TCATCCCTTT CTGCGGGAAG 
GGAGGCCTCT TCAAGTATGA GATCCTGGTC AAGACCGGCT GGGGCCGAGG CTCAGGTACC 
ACGGCACACG TGGGCATCAT GCTGTACGGG GCGGAGGGCC GCAGCGGCCA CCGGCACCTG 
GATGGGGACA GAGCCTTCCG CCGCAACAGC CTGGACGTGT TCCAGATCGC CACCCCGCAC 
AGCCTGGGCA GCGTGCGCAA GATCCGCGTG TGGCACGACA ACAAAGGCCT CAGCCCCGCC 
TGGTTCCTGC AGCACATCAT TGTCAGGGAC CTGCAGAACG CCCGCAGCAC CTTCTTCCTG 
GTCAATGACT GGCTGTCGGT GGAGACTGAG GCCAACGGCG GCCTCGTAGA GAAGGAGGTG 
CTGGCAGCAA GCGAGGCGGC CCTGCGGCGG TCCCGGCGCC TCCTCGTGGC CGAGCTGCAG 
CGCGGCTTCT TTGACAAGCA CATCTGGCTC TCCATATGGG ACCGGCCGCC TCGGAGCCGC 
TTCACTCGTG TCCAGCGGGC CACATGCTGC ATCCTCCTCG TCTGCCTCTT CCTGGGCGCC 
AATGCTGTGT GGTACGGGGT CGTGGGAGAC ACCGCCTCCA GCGCGGGGCC TGTGTCCACT 
CTCATCCCGT TGAGTGTTGA CACGATCGCC GTCGGCCTGG TGTCCAGTGT GGTCGTCTAT 
CCCGTCTACC TGGTCATCCT GTTTCTCTTC CGGATGTCCC GGAGCAAGGT GGCCGGGGCC 
CCGAGCCCCA CCCCTTCAGG GCAGCAGGTA CTGGACGTTG ACAGCTACCT GGACTCGTCT 
GTGCTGGATA GCTCCTTCCT CACCTTCCCG GGGCTGCGCA CAGAGGCCTT TGCTGGACAA 
ATGAAAAGCG ACTTATTTTT GGATGATTCT AAAAGCCTGG TGTGCTGGCC ATCCAGTGAA 
GGCACACTTA ACTGGCCGGA CCTGCTGAGT GACCCGTCCA TCATGGGCAG CACCCTGCAG 
CGGCTGGCTC GGGGCCGCAC GGGCCACACG CTGGGCCCAG AGGACGACGG TCTCTCCTTG 
CTGAGCCCCT CTTCACCTGC CAAATACTTC TCAGCTTCAG ATGAGGACCT GATCCAGCAG 
ATCCTGGCCG AAGGCGCCAG CAGCCTGGCC CCCACCCAGG ACACCCAAGT GGAAACGGAT 
CTGCTCACCA GCCTGTCTAG CGCCCCCGGG GAGACAACGG AAACGCTGAC GCTGCAGAGG 
CTGGGGGAGA GGGGGCCGCC GTGCAGCTCC GGCGTGACCT GGGAGCAGCC CACGTCGGCC 
AGGCTCTCCG GGACAGGGCT GGCGGACGGT CTGCAGAAGC GGCTGCTGCC CGCCTGGTGT 
GCGTCCCTGG CCCACGGGCT CAGCTTGCTC TTGGCTGCTG TGGCTGTGGG GGTCTCTGGC 
TGGGTAGGCT CCAGCTTCCC CCCTGGTGTG AGCGTGATGT GGCTCCTCTC AAGCAGCTCC 
AGTTTCTTGG CCTCCTTCCT TGGCTGGGAG CCCCTCAAGG TCCTGCTGGA AGCCCTGTAC 
TTCTCGCTGG TGGCCAAGCG GCTCCATCCT GATGAGGATG ATACCCTGGT GGAGAGCCCG 
GCTGTGACCC CTGTGAGCGA GCGTGTGCCT CGCGTGAGGC CCCCCCACGG CTTTGCGCTC 
TTCCTGGCAA AGGAGGAAGC CCGAAAGGTC AAGAGGCTGC ATGGGATGCT GAGGAGCCTG 
CTGGTGTATA TGCTCTTTTT GCTGGTGACG CTGTTGGCCA ACCACGGGGA TGCCTCATGT 
CACAACCATG CCTACCGCTT ACAGAGTGCC ATCAAGCAGG AGCTGGGCAG CCAGGCCTTC 
CTGGCCATCA CCCGGTCTGA TGAGTTCTGG CCATGGATGT CACACGTGCT GCTTCCCTAC 
ATCCATGGGA ACCAGTCCGG CCCAGAGCTG GGGCCCCCAC GGCTGCGGCA GGTGCGGCTG 
CAGGAAGCTC TGTGCCCAGA CCCTCCCGGC GCCGCGGTGC ATGCGTGCTC AGCCGGCCAG 
GACGCCTTCA GCACCGGGGA CTACAGCATT GGCTGGGGTA GTGCTGCCCA GAACTGCTCC 
GAGACGTGGG CCTACTCTGC GCCAGACCTA CTGGGCATCT GGTCCTGGGG CTACTGCGCT 
GTGTACGACA GCGGGGGCTA TGTGCAGGAG CTAGGGCTGA GCCTGGAGGA GAGCCGCGCG 
CAGCTGGGCT TCCTGCAGCT GCATAACTGG ATCGACAACA GGAGCCGCGC CGTGTTCGTG 
GAGCTCACGC GCTACAGCCC AGCCGTGGGG CTGCACGCCG CCGTCACGCT GCGCCTCGAG 
TTCCCCGCTG CCGGGCACGC GGTGACCGCT GTCAGCGTGC GTCCCTTCCC GCTGCAGCGC 
CTGAGCACCG GCCTCTCCCT GCCGCTGCTC ACCTCGGTGA GCCTGCTGCT CTTCGCGCTG 
TACTTCTCCG TGGCCGAGGT GCGCACCTGG CGCAGGGAAG GGTGTAGACG TGCGGCGCAG 
CCCGGGGCGT GGGCGCGGTG GCTGCTGGTG GCACTGACCG CGGCCGCAGC GCTCGTGCGC 
CTGGCCCAGC TGGGTGTTGC CGACCGCCAG TGGGCCCGCT TCTTGCGCCG CCGTCCGCGC 
CGATTCACCA GCTTCGAGCA GGTGGCGCAG CTGAGCGCCG CGGCTCGCGG CCTGGCCGCC 
TCGCTGCTCT TCCTGCTCCT GGTCAAGGCC GCGCAGCAGC TGCGCTTTGT GCGCCAGTGG 
TCGGTTTTTG GCAAGACGCT GTGCCGGGCC CTGCCGGAGC TCGTGGGGGC AGCCCTCGGC 
CTGGTGGCGC TCGCCGTGGC CTACGCGCAC CTGGCCGTTC TGCTGGTCTC CTCCTGTGTG 
GATTCATTCC GGAGTGCGGC GCGGGCCCTC TTGGTGCTGT GCCCTGGGTC AGGGGGTCCC 
GCCCTGTGCC CTGCCGAGTC CTGGCGCCTG TCCCCGCTGC TGTGCACCGG GCTCTGGGCC 
CTGCGCCTTT GGGGCGCCCT GCGGCTGGGG GCCGTCCTTC TGAGATGGCG GTACCATGCC 
CTGCGTGGGG AACTCTACCG ACCGGCCTGG GAGCCGCAGG ACTACGAGAT GGTGGAGCTC 
TTTCTGCGCA GGCTGCGCCT CTGGATGGGT TTCAGCAAGG TCAAGGAGTT CCGCCACAAA 
GTCCGCTTTG AAGGGATGGA GCCGCTGCCC TCCCGCTCCT CCAGGGGCTC CAAGTCCTCC 
CCGGATGCAC CCCCACCCAG CGGGGGCTCG GACACCTCAC GGCCCTCCAC GTCCTCCAGC 
CAGCTGGACA CGCTGAGTGG AGGCCTGGGC CGCCTGGGGC CCCGGGGGGA GCCGGAGCCC 
TCTCGCCTGC AAGCGGTGTT CGAGGCCCTC CTGACCCAGT TCGACCGACT CAACCAGGCC 
ACAGAGGATG TGTACCAGCT GGAGCAGAGG CTGCAGAGCC TGCGGGGCCG CAGGAGCACC 
GTTCCCCCAG CCTCGCCTCC CCACAGCCCC TGCTCAGCCC TGCAGCCGGC CCTGCCCAGC 
CGTCTTGCCC GGGCCAGTCG AGGCATGGGC CTGGCATCCG GCCCCAGCAG GGCTTCCCTG 
CGGGCTAAGA ACAAGGTCCA CCCCAGCAGC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006651.1
CDS	8..12943
Translation	MPPAAPGRLALALGLGLWLGALAGGPGRGCGPCAPPCLCGPAPGAACRVNCSGGGLRTLGPALRIPAAATALDVSHNLLRALDVGLLANLSALTELDISNNKISTLEEGIFSNLFNLSEINLSGNPLECNCGLAWLPRWAKEQQVHVVRPEAATCAGPGPLAGQPLLGVPLLDGGCGEEYIACLPDNSSGAVALVAFSAAHEGPLTPEACGAFCFAAGNGLGAFSDQGWCLCGAAQPPNTSSACLPFCSGAPLALAPACGGPTLLQNIFPASPGAALVGPQGPLASGQPAAFHVTASLPVSSTRWDFGDGSPKVDIAGPATTHRYLLPGLYHVTVVLALGAGSAQVETQVHVEAAPASLELTCLTSVRSDETLKFGIRNRGGSDLEATYSIVAVGEEPAQDPAVVHPLCPSDTVIFPGNGHCYRLVAEKAAWLQAQEQCRAWAGAALAMVDSPAIQHFLVSQVTRSLDVWIGFSAVEGAEAGPVVQGAAFSLESCQNWLPGEPHPATAERCVRLGPAGQCNTDLCSAPHSYVCELRPGGPVWDAENFLVGAPIGDLQGPLSPLAQQEALSAPQELIEVMVFPSLGLSREAFLTAAEFGTQELRRPTQLRLQVFRPVGEAGAPGNSREPESGSRESRTESVPECTPEGRWCPRASIHLPPDDPCGPWACANGSMSGPGLPGASCVLLREFLFSVPAGPPSQYSVTFRGQDGPLKPGDLIALQHDAGPGALLCCSPALGAPGAEAPYFSATSPAWLVHLPAQLEAAPAGPACALRLLVATERLTTLLGLRPNPGLRRPGRYEVRATVGNSVSTHNLSCSFDVLSPVAGLRVAHPIPHDGRLYVPTNSSVLVLQVDSGTNATATARWPGGNASAPFEATCPATVAALVPSCTLEANDTLFAVLVLPGLSEGEHAVEIMVENSAGHANLSLRVKAEEPICGLRAAPSPEARVLQGVLVRYSPVVEAGSDVVFRWTIDDKQSLTFHNVVFNVIYQSAAVFKLSLTASNHVSNVTVNYNVTVERMHKMKGLRVSAVPPVLPPNTTLELAAHVLVDSAVEVAFLWTFGDGDQVLGQFKPPYNESWVPDPTVAQVLVEHSVTHVYAVPGEYNLTLLVSNAFENLTQQVPVSVRATLPAVAVAVGSHVLVAGQPVTFSPHPLPSPGGILYTWDFGDGSPAVTQHQTEVSHTYASRGIYRIHLEVNNTVSSVTAWTSVRVFEELRGLSVSLSPSVEQGAPVTVSATLESGDNVTWTFDMGDGTVLVGPEAMVEHVYLRAQNHTVTVGVSSPAGYLALSLPVHVFVLEVLRIEPAACIPVQPHTWFMAHVTGDPAHYIFDWTFGDGSSNTTIFGDPTVTHNFTRSGTFPLALVLSSHVNKAHYFTSVCVEPEVGNVTLWPERQFVRLGDEAHLVARAWPPFPYRYTWDFGPEDAPRVGGPEATFTYADPGSYLVTVAVSNNISAANDSALVEVEEPVELTGIGVNGSGVLELQRPYLFSAVGRGHPATYLWELGDGGRLEGPSIKHTYNSTGHFTVRVSAWNEVSRGEAWLNITVQRRVQGLSVNASRTVVPLNGSVSFTTSLEAGSDVRYSWVLCDRCTPIPGGPTISYTFRSVGTFNIIVTAENEVGSAQDSIFIYVLRHIEGLQVAGGGGGCCFPTNCTLQLQAVVSDGTNIFYSWSAQRDGGPALAGSGKAFSLTVLEAGTYHVQLRAANMLGTSWANRTVDFVEPVGALAAAASPNPAIVNSSVTLCAELAGGSGVTYTWSPEGGLSWETPEPSSTHVFPSPGLYLVRVTATNWLGSANVTIEVAVQVPVSGLSIRAGDIDRGFVAAGSAVPFWGQLDSGTNVSWCWTVPGGSRRGQHVAVVFRDAGAFSVRLNASNAVSWVTATRELTVEEPIAGLVVWADSKVVEPGQLVHFQVMLAAGSAVRFHLQVSGAAVEALPGPRFSRSFPRVGDYMVSVQAENHVSRAQAQVRILVLEAVGGLQVPHCCELGIPTGTERNFTARVQRGSRVAYAWYFSLQKVQGDSLVILSGRDVTYTPVAAGLLEIHVRAFNDLGGVNLTLLMEVQDAIQHVVLRSGRCFTNRTARFEAATSPSPRRVAYRWDFGDGAVVEDTEEPWAEHSYLRPGDYRIEVNASNLVSFFVAQATVTVHVLACREPEVDVALPPQVLMRRSQRNYLEAHVNLRDCVTYQTEYRWQVYRAASCQRPGRLAPVALPSVDMSRPQLVVPRLALPVGHYCFVFMVSFGDTPLARSIQANVTVVPERLVPIVEGGSYRVWSDTQDLVLDGSKSYDPNLEDGDQTPLSFHWACVASTQSETGGCTLTFGPRGSSVVIVPRERLQAGVEYTFNLTVWKAGRKEEATNQTVLIRRGRVPIVSLECVSCKAQSVYAVSRSSYVYLEGRCDNCSEGSKRGRWAARTFSNQTLVLDETTTSTGSSGMRLVLRRGVLRDGEGYTFTLTVLGRSGEEEGCASIRLSANRPPRGGSCRLFPLDAVRALTTKVHFECTGWQDAEDASAPLVYALLLRRCRQGHCEEFCVYRGSLSAYGAVLPPGFAPHFQVGLAVVVQDQLGAAVVALNRSLAITLPQPPGDPQGGPTDLTSWLHGLTESMLPRLLRQADPQHVIEYSLALITVLNEASAGSEQVLAVVAEEDARRQLQAQIRKNVTETLVSLRVNTVDDIQQIAAALAQCTVSSRELVCHSCLKKTLHKLEAMMRILQAETAAGTGTPTGIADSILNITGDLIHLASVDMQGLQPSELGTEPPSLMVASRAYHLSSALMCILMRSRVLNEEPLTLAGEEIVAQGKRSDPLSLLCQGNASVPSCHFSIPTAFSGALSNLSDVVQLMFLVDSNPFPFGYISNYTVSTKVASMAFQTQAGTQIPIGQLASERAIIVKVPNSSDQAAPGHRAPAGSAVIQPQASISVAVTPENSNPAAGLHLQLTYTLLSERYLPEEPEPYLAVYFDSVPGPNEHNCSASRRISLEELAGGDHRPYTFFIAPGTRDSGGTYYLNLTSRFHWSALEVSVGLYTSLCQYFSEKEMMWKTEGLVPLEETSPSQAVCLTRHLTAFGASLFVPPSHVHFIFPEPASGVNYIVLLTCIVCLVTYTVMAVILRKLDQLDVSRVRIIPFCGKGGLFKYEILVKTGWGRGSGTTAHVGIMLYGAEGRSGHRHLDGDRAFRRNSLDVFQIATPHSLGSVRKIRVWHDNKGLSPAWFLQHIIVRDLQNARSTFFLVNDWLSVETEANGGLVEKEVLAASEAALRRSRRLLVAELQRGFFDKHIWLSIWDRPPRSRFTRVQRATCCILLVCLFLGANAVWYGVVGDTASSAGPVSTLIPLSVDTIAVGLVSSVVVYPVYLVILFLFRMSRSKVAGAPSPTPSGQQVLDVDSYLDSSVLDSSFLTFPGLRTEAFAGQMKSDLFLDDSKSLVCWPSSEGTLNWPDLLSDPSIMGSTLQRLARGRTGHTLGPEDDGLSLLSPSSPAKYFSASDEDLIQQILAEGASSLAPTQDTQVETDLLTSLSSAPGETTETLTLQRLGERGPPCSSGVTWEQPTSARLSGTGLADGLQKRLLPAWCASLAHGLSLLLAAVAVGVSGWVGSSFPPGVSVMWLLSSSSSFLASFLGWEPLKVLLEALYFSLVAKRLHPDEDDTLVESPAVTPVSERVPRVRPPHGFALFLAKEEARKVKRLHGMLRSLLVYMLFLLVTLLANHGDASCHNHAYRLQSAIKQELGSQAFLAITRSDEFWPWMSHVLLPYIHGNQSGPELGPPRLRQVRLQEALCPDPPGAAVHACSAGQDAFSTGDYSIGWGSAAQNCSETWAYSAPDLLGIWSWGYCAVYDSGGYVQELGLSLEESRAQLGFLQLHNWIDNRSRAVFVELTRYSPAVGLHAAVTLRLEFPAAGHAVTAVSVRPFPLQRLSTGLSLPLLTSVSLLLFALYFSVAEVRTWRREGCRRAAQPGAWARWLLVALTAAAALVRLAQLGVADRQWARFLRRRPRRFTSFEQVAQLSAAARGLAASLLFLLLVKAAQQLRFVRQWSVFGKTLCRALPELVGAALGLVALAVAYAHLAVLLVSSCVDSFRSAARALLVLCPGSGGPALCPAESWRLSPLLCTGLWALRLWGALRLGAVLLRWRYHALRGELYRPAWEPQDYEMVELFLRRLRLWMGFSKVKEFRHKVRFEGMEPLPSRSSRGSKSSPDAPPPSGGSDTSRPSTSSSQLDTLSGGLGRLGPRGEPEPSRLQAVFEALLTQFDRLNQATEDVYQLEQRLQSLRGRRSTVPPASPPHSPCSALQPALPSRLARASRGMGLASGPSRASLRAKNKVHPSST

Target ORF information:

RefSeq Version	NM_001006650.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris polycystin 1, transient receptor potential channel interacting (PKD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006650.1

ORF Insert Sequence:

ATGCCGCCCG CCGCGCCCGG CCGCCTGGCG CTCGCCCTGG GCCTGGGCCT GTGGCTCGGG 
GCGCTGGCGG GGGGCCCCGG GCGCGGCTGC GGCCCGTGCG CGCCCCCCTG CCTGTGCGGC 
CCTGCGCCCG GCGCCGCCTG CCGCGTCAAC TGCTCGGGCG GCGGACTGCG GACGCTCGGA 
CCCGCGCTGC GCATCCCCGC CGCCGCCACC GCGCTAGACG TCTCCCACAA CCTGCTCCGG 
GCATTGGACG TTGGGCTCCT GGCAAACCTC TCTGCACTGA CAGAGCTGGA TATCAGTAAC 
AACAAGATTT CAACTCTAGA AGAAGGAATA TTTTCTAACT TATTTAACTT AAGTGAAATA 
AACCTGAGCG GAAACCCGTT GGAGTGTAAC TGCGGTCTGG CGTGGCTGCC TCGCTGGGCG 
AAAGAGCAGC AGGTTCACGT GGTTCGGCCT GAGGCGGCCA CGTGTGCTGG GCCCGGCCCC 
CTAGCTGGCC AGCCCCTCCT CGGGGTCCCC TTGTTGGATG GTGGCTGTGG TGAGGAGTAC 
ATTGCCTGCC TCCCTGACAA CAGCTCGGGT GCTGTGGCGC TGGTGGCCTT CTCAGCCGCC 
CACGAGGGCC CACTGACGCC CGAGGCCTGC GGTGCCTTCT GTTTTGCGGC TGGCAATGGC 
CTTGGGGCCT TCTCAGACCA GGGCTGGTGC CTGTGCGGGG CCGCCCAGCC CCCCAACACC 
TCCTCAGCCT GCCTGCCCTT CTGCTCCGGC GCCCCACTGG CCCTTGCCCC TGCGTGTGGG 
GGCCCCACTC TCCTCCAGAA CATCTTCCCC GCCTCCCCAG GGGCCGCCCT GGTGGGGCCT 
CAGGGACCCC TGGCCTCTGG CCAGCCAGCA GCCTTCCATG TCACTGCCTC CCTGCCCGTC 
AGCTCCACAC GCTGGGACTT CGGTGATGGC TCCCCCAAGG TGGACATTGC TGGTCCTGCC 
ACCACTCACC GCTACCTGCT GCCCGGGCTC TATCACGTGA CTGTTGTGCT GGCCCTGGGG 
GCTGGCTCAG CCCAGGTAGA GACCCAGGTG CACGTGGAGG CGGCCCCTGC CTCCCTGGAG 
CTCACGTGCC TCACCTCGGT GCGCAGCGAT GAGACCCTCA AGTTTGGCAT CCGAAATCGT 
GGCGGCTCGG ACCTGGAAGC CACATACAGC ATTGTGGCTG TGGGCGAGGA GCCAGCCCAA 
GACCCCGCAG TGGTGCATCC GCTGTGCCCC TCGGACACCG TCATCTTCCC TGGTAATGGG 
CACTGCTACC GTCTGGTGGC AGAGAAGGCT GCCTGGCTGC AGGCGCAGGA GCAGTGCCGG 
GCCTGGGCTG GGGCCGCCCT GGCCATGGTG GACAGTCCCG CCATCCAGCA CTTCCTGGTC 
TCCCAGGTCA CCAGGAGCCT GGACGTGTGG ATCGGCTTCT CAGCCGTGGA GGGGGCGGAG 
GCGGGCCCGG TGGTGCAGGG TGCCGCCTTC AGCCTCGAGA GCTGCCAGAA CTGGCTCCCC 
GGGGAGCCGC ACCCCGCCAC CGCCGAGCGC TGCGTGCGGC TCGGGCCTGC GGGCCAGTGC 
AACACGGACC TGTGCTCGGC GCCCCATAGC TACGTCTGCG AGCTGCGGCC TGGAGGTCCC 
GTGTGGGACG CGGAGAACTT CCTCGTGGGA GCGCCCATTG GGGACCTGCA GGGACCCTTG 
AGCCCCCTGG CACAGCAGGA GGCCCTCTCG GCCCCCCAGG AGCTTATCGA GGTGATGGTG 
TTCCCCAGCC TGGGCCTGAG CAGGGAAGCC TTCCTCACTG CAGCTGAGTT TGGCACCCAG 
GAGCTGCGCC GGCCCACCCA GCTGCGACTG CAGGTGTTTC GGCCCGTGGG AGAAGCAGGG 
GCCCCAGGGA ACAGCAGGGA GCCTGAGAGT GGGTCCCGGG AGAGCCGGAC TGAGTCAGTC 
CCTGAGTGCA CCCCAGAGGG ACGCTGGTGC CCCAGAGCCA GTATCCACCT GCCACCAGAT 
GACCCCTGCG GCCCCTGGGC CTGTGCCAAT GGGTCCATGT CTGGGCCAGG ACTCCCCGGG 
GCCTCCTGTG TGCTGCTGAG GGAGTTCCTC TTCTCTGTAC CGGCGGGTCC TCCTTCCCAG 
TACTCAGTCA CCTTCCGTGG CCAGGACGGG CCCCTGAAGC CTGGTGACCT CATCGCCCTG 
CAGCATGACG CGGGACCAGG TGCCCTTCTG TGCTGCTCTC CAGCACTTGG TGCCCCTGGG 
GCTGAGGCCC CCTACTTTTC CGCCACCTCC CCGGCCTGGC TGGTGCACCT CCCTGCTCAG 
CTGGAGGCAG CCCCGGCTGG CCCAGCCTGT GCCTTGCGGC TGCTTGTGGC TACAGAGCGC 
CTCACCACCC TCCTGGGCCT GCGGCCCAAC CCAGGGCTGC GGCGGCCTGG GCGCTATGAG 
GTCCGGGCCA CCGTGGGCAA CAGCGTGTCC ACACACAACC TGTCCTGCAG CTTTGACGTG 
CTCTCCCCCG TAGCTGGGTT GCGTGTCGCC CACCCGATCC CCCACGACGG CCGCCTCTAC 
GTGCCCACCA ACAGCTCAGT CTTGGTGCTC CAGGTGGACT CTGGCACCAA CGCCACCGCC 
ACGGCCCGCT GGCCTGGGGG CAATGCCAGT GCACCCTTTG AAGCCACCTG CCCTGCCACA 
GTGGCTGCCC TGGTACCCAG CTGCACTCTT GAGGCCAATG ACACCCTGTT TGCGGTGCTT 
GTGCTGCCTG GGCTCAGCGA GGGTGAGCAC GCGGTGGAGA TCATGGTGGA GAACAGCGCC 
GGCCACGCCA ACCTCAGCCT GCGGGTCAAG GCCGAGGAGC CCATCTGTGG CCTTCGTGCC 
GCCCCCAGCC CCGAAGCCCG TGTGCTGCAG GGAGTCCTGG TGAGGTACAG CCCCGTGGTG 
GAGGCCGGCT CTGATGTGGT CTTCCGCTGG ACCATTGATG ACAAGCAGTC CCTGACCTTC 
CACAACGTGG TCTTCAACGT CATCTACCAA AGTGCTGCCG TCTTCAAGCT CTCGCTGACG 
GCCTCCAACC ACGTGAGCAA CGTCACCGTA AATTACAATG TCACCGTGGA GCGGATGCAC 
AAGATGAAGG GCCTTCGGGT GTCTGCCGTG CCACCCGTGT TGCCTCCCAA CACGACTCTG 
GAGCTGGCCG CGCACGTGCT GGTGGACTCA GCTGTGGAGG TGGCCTTTCT GTGGACCTTT 
GGGGATGGGG ATCAGGTGCT CGGCCAGTTC AAGCCTCCAT ACAATGAGTC CTGGGTCCCA 
GACCCCACGG TGGCCCAGGT GCTGGTGGAG CACAGTGTCA CACACGTCTA TGCTGTCCCA 
GGTGAGTACA ACCTGACCCT GCTGGTGTCC AACGCCTTTG AGAACCTGAC GCAGCAAGTG 
CCTGTGAGTG TGCGCGCCAC CTTACCTGCG GTGGCTGTGG CTGTGGGAAG CCACGTCCTG 
GTGGCTGGGC AGCCTGTCAC CTTCTCCCCA CACCCACTGC CTTCACCTGG GGGCATTTTG 
TACACGTGGG ACTTTGGGGA TGGCTCCCCA GCTGTGACGC AGCACCAAAC AGAGGTCAGC 
CACACATATG CCTCGAGGGG TATTTACCGC ATCCATCTGG AGGTGAACAA CACGGTTAGC 
AGTGTGACAG CGTGGACCAG CGTCCGCGTC TTTGAGGAGC TCCGAGGCCT GAGTGTGAGC 
CTGAGCCCAT CTGTGGAACA GGGTGCGCCC GTGACGGTCA GCGCCACCCT GGAGTCGGGA 
GACAACGTCA CGTGGACTTT TGACATGGGG GATGGCACCG TGCTTGTGGG CCCCGAGGCC 
ATGGTGGAAC ACGTTTACCT GCGGGCACAG AACCACACGG TGACCGTGGG CGTGTCCAGC 
CCTGCGGGCT ACCTGGCCCT GAGCCTGCCT GTGCACGTCT TCGTCCTGGA GGTGCTGCGG 
ATTGAGCCTG CGGCCTGCAT CCCTGTGCAG CCCCACACCT GGTTCATGGC CCACGTCACC 
GGGGACCCTG CCCACTACAT CTTTGACTGG ACGTTCGGGG ATGGGTCATC CAACACGACC 
ATCTTTGGGG ACCCAACGGT GACACACAAT TTCACACGGA GCGGAACGTT CCCCTTGGCG 
TTGGTCCTAT CAAGCCATGT GAACAAGGCC CATTACTTCA CCAGCGTCTG CGTGGAGCCC 
GAGGTGGGCA ACGTCACCCT GTGGCCCGAG AGGCAGTTTG TGCGCCTCGG GGATGAGGCC 
CATCTGGTGG CTCGCGCCTG GCCCCCCTTC CCCTACCGTT ATACCTGGGA CTTTGGCCCT 
GAAGACGCGC CCCGTGTTGG GGGCCCCGAG GCCACGTTCA CCTATGCGGA CCCAGGTTCC 
TACCTGGTGA CGGTCGCTGT CTCCAACAAC ATCTCGGCCG CCAATGACTC TGCACTCGTG 
GAAGTGGAGG AGCCCGTGGA GCTCACGGGC ATTGGGGTCA ACGGCTCTGG TGTGCTCGAG 
CTGCAGCGGC CCTACCTGTT CTCTGCTGTG GGCCGCGGAC ACCCCGCCAC CTACCTGTGG 
GAACTGGGGG ACGGCGGGCG CCTCGAGGGC CCCTCCATCA AGCACACCTA CAACAGCACA 
GGCCACTTCA CCGTCAGGGT GAGCGCGTGG AATGAGGTGA GCCGCGGCGA GGCCTGGTTG 
AACATCACGG TGCAGCGGCG CGTGCAGGGG CTCAGCGTCA ACGCCAGCCG CACGGTGGTG 
CCCCTCAACG GCAGCGTGAG CTTCACCACC TCCCTGGAGG CCGGCAGCGA CGTGCGCTAC 
TCCTGGGTGC TCTGTGACCG CTGCACGCCC ATCCCTGGGG GCCCCACCAT CTCCTACACC 
TTCCGCTCCG TGGGCACCTT CAACATCATC GTGACCGCTG AGAACGAGGT GGGCTCTGCC 
CAGGACAGCA TCTTCATCTA CGTGCTGCGG CACATCGAGG GGCTGCAGGT GGCAGGGGGT 
GGGGGCGGCT GCTGCTTCCC CACCAACTGC ACGCTGCAGC TGCAGGCCGT GGTCAGCGAC 
GGCACCAACA TCTTCTACAG CTGGAGCGCC CAGAGGGATG GCGGCCCGGC CCTGGCTGGC 
AGCGGCAAAG CCTTCTCGCT CACCGTGCTC GAGGCCGGCA CATACCACGT CCAACTGCGG 
GCTGCCAACA TGCTGGGCAC GTCCTGGGCC AACCGCACGG TGGACTTCGT GGAGCCCGTG 
GGCGCACTGG CTGCCGCCGC CTCCCCAAAC CCGGCCATAG TCAATTCCAG CGTCACCCTC 
TGTGCCGAGC TGGCTGGGGG TAGTGGTGTC ACTTACACCT GGTCCCCGGA GGGAGGACTG 
AGCTGGGAGA CCCCAGAGCC ATCCTCCACT CACGTCTTCC CCAGCCCCGG CCTGTACCTG 
GTCAGGGTGA CAGCTACGAA CTGGCTGGGC TCAGCCAATG TGACCATCGA GGTGGCTGTG 
CAGGTGCCGG TCAGTGGCCT CAGCATCCGG GCCGGCGACA TAGACCGCGG CTTCGTGGCA 
GCAGGCTCTG CGGTGCCCTT CTGGGGACAG CTGGACTCAG GCACCAACGT GAGCTGGTGC 
TGGACGGTGC CAGGGGGCAG CAGGCGTGGC CAGCACGTTG CTGTGGTCTT CCGTGACGCA 
GGCGCCTTCT CCGTGCGGCT CAATGCCTCC AATGCAGTCA GCTGGGTCAC GGCCACCCGT 
GAGCTCACGG TGGAGGAGCC CATTGCGGGC CTGGTGGTGT GGGCCGACAG CAAGGTGGTG 
GAGCCGGGGC AGCTGGTCCA CTTCCAGGTC ATGCTGGCTG CCGGCTCCGC TGTGCGCTTC 
CATCTGCAGG TCAGCGGGGC CGCTGTGGAG GCGCTCCCCG GGCCCCGCTT CTCCCGCAGC 
TTCCCCCGAG TTGGGGACTA CATGGTGAGC GTCCAGGCTG AGAACCACGT GAGCCGGGCC 
CAGGCCCAGG TGCGCATCTT GGTGCTCGAG GCTGTGGGTG GGCTCCAGGT GCCCCACTGT 
TGCGAGCTGG GCATCCCCAC GGGCACGGAG AGGAATTTTA CAGCCCGGGT GCAGCGTGGG 
TCGCGCGTGG CCTACGCCTG GTACTTCTCC TTGCAAAAGG TCCAGGGGGA CTCACTGGTC 
ATCCTTTCGG GCCGTGATGT CACCTACACC CCCGTGGCCG CGGGGCTGCT GGAAATCCAC 
GTGCGTGCCT TCAATGATCT GGGCGGCGTG AACCTCACGC TGCTGATGGA GGTCCAGGAT 
GCCATCCAGC ATGTGGTGCT GCGCAGTGGC CGCTGCTTTA CCAACCGCAC AGCCCGGTTT 
GAGGCCGCCA CAAGCCCTAG TCCCCGGCGT GTGGCCTATC GCTGGGACTT TGGGGACGGG 
GCCGTGGTGG AGGACACGGA GGAGCCCTGG GCTGAACACT CCTATCTGCG GCCGGGGGAC 
TACCGCATAG AGGTGAATGC CTCCAACCTC GTGAGCTTCT TCGTGGCCCA GGCCACGGTC 
ACTGTCCACG TGCTGGCCTG CAGGGAGCCC GAGGTGGACG TGGCCCTGCC CCCACAGGTG 
TTGATGCGGC GCTCCCAGCG CAACTACCTG GAGGCCCACG TCAACCTGCG CGACTGTGTC 
ACCTACCAGA CCGAGTACCG CTGGCAGGTG TACCGTGCTG CCAGCTGCCA GCGGCCTGGG 
CGCCTGGCCC CTGTGGCCCT GCCCAGTGTG GACATGAGCC GGCCCCAGCT GGTTGTGCCA 
CGGCTGGCAC TGCCTGTGGG CCACTACTGC TTTGTGTTCA TGGTATCATT TGGAGACACA 
CCGCTGGCAC GGAGCATCCA AGCCAATGTG ACTGTGGTGC CTGAGCGCCT GGTGCCCATT 
GTTGAGGGTG GCTCATACCG CGTGTGGTCA GACACTCAGG ATCTGGTGCT GGATGGGAGC 
AAATCCTATG ATCCCAACCT AGAGGATGGT GACCAGACAC CACTCAGCTT CCATTGGGCC 
TGTGTGGCCT CGACACAGAG TGAGACTGGT GGCTGTACCC TGACCTTCGG GCCCCGGGGA 
AGCAGCGTGG TCATCGTTCC TCGGGAGCGC CTGCAGGCTG GCGTGGAGTA CACCTTCAAC 
CTGACTGTGT GGAAGGCCGG CCGGAAGGAG GAGGCCACCA ACCAGACGGT GTTGATCCGC 
AGGGGCCGCG TGCCCATCGT GTCCTTGGAG TGCGTGTCCT GCAAGGCGCA GTCGGTGTAC 
GCAGTGAGTC GAAGCTCCTA CGTGTACCTG GAGGGCCGCT GTGATAACTG CAGCGAGGGT 
TCCAAGCGAG GGCGCTGGGC GGCGCGCACC TTCAGCAACC AGACGCTGGT GCTGGACGAG 
ACGACCACGT CGACGGGCAG CTCGGGCATG CGGCTGGTGC TGCGGCGCGG CGTGCTGCGG 
GACGGCGAGG GCTACACGTT CACACTCACC GTGCTGGGCC GCTCGGGCGA GGAGGAGGGA 
TGCGCCTCCA TCCGCCTGTC CGCCAACCGG CCCCCGCGCG GGGGCTCCTG CCGCCTCTTC 
CCGCTTGACG CCGTTCGCGC CCTCACCACC AAGGTGCATT TCGAGTGCAC AGGCTGGCAG 
GACGCCGAAG ACGCGAGCGC CCCACTGGTG TACGCACTGC TGCTGCGGCG CTGTCGCCAG 
GGCCACTGCG AGGAGTTCTG CGTGTACAGG GGCAGCCTCT CTGCCTACGG AGCTGTGCTG 
CCGCCCGGTT TCGCACCGCA CTTCCAGGTG GGCCTGGCCG TGGTCGTGCA GGACCAGCTG 
GGCGCAGCTG TGGTCGCTCT CAACAGGTCT CTGGCCATCA CCCTGCCACA GCCTCCTGGC 
GACCCCCAGG GAGGACCCAC AGACCTCACC AGCTGGTTGC ACGGCCTCAC GGAGAGCATG 
CTGCCCAGGC TGCTGAGACA GGCCGACCCC CAGCACGTCA TCGAGTACTC GCTGGCCCTC 
ATCACTGTGC TCAATGAGGC CAGTGCAGGG TCCGAGCAGG TTCTGGCCGT GGTGGCAGAG 
GAGGATGCCA GGCGGCAGCT GCAAGCCCAG ATCCGCAAGA ATGTCACAGA GACTTTGGTC 
TCCCTGCGGG TCAACACCGT GGATGACATC CAGCAGATCG CGGCAGCGCT GGCCCAGTGC 
ACGGTGTCCA GCAGGGAGCT CGTGTGCCAC TCCTGCCTGA AGAAGACGCT GCATAAGCTT 
GAGGCCATGA TGCGCATCCT GCAGGCTGAG ACGGCTGCTG GCACTGGGAC GCCCACGGGC 
ATAGCTGACA GCATTCTCAA CATCACAGGC GACCTCATCC ACTTGGCCAG TGTGGACATG 
CAGGGCCTGC AGCCCTCGGA GCTGGGCACA GAGCCGCCCT CACTCATGGT AGCATCCAGG 
GCCTACCACC TCTCATCGGC CCTCATGTGC ATTCTCATGC GCTCCCGTGT GCTCAACGAG 
GAGCCCCTGA CGCTGGCGGG TGAGGAGATT GTGGCCCAGG GCAAGCGCTC CGACCCGCTG 
AGCCTGCTGT GCCAGGGCAA CGCCTCGGTT CCCAGCTGCC ACTTCTCTAT CCCCACGGCC 
TTCAGCGGGG CCCTGTCCAA CCTCAGTGAT GTGGTGCAGC TCATGTTCCT CGTCGACTCC 
AATCCATTCC CCTTTGGCTA CATCAGCAAC TACACCGTCT CCACCAAGGT GGCCTCCATG 
GCCTTCCAGA CGCAGGCCGG CACCCAGATC CCCATCGGGC AGCTGGCCTC AGAGCGCGCC 
ATCATCGTGA AAGTGCCCAA CAGCTCCGAC CAGGCTGCCC CGGGCCACCG CGCCCCTGCT 
GGCTCCGCAG TCATCCAGCC CCAGGCCTCC ATCAGCGTTG CGGTCACCCC TGAGAACAGC 
AACCCGGCGG CCGGGCTGCA CCTGCAGCTC ACCTACACGC TGCTCAGTGA GCGCTACTTG 
CCCGAGGAGC CTGAGCCCTA CCTGGCTGTG TACTTTGACT CGGTGCCTGG GCCCAACGAG 
CACAACTGCT CCGCCAGCAG GAGGATCAGC CTGGAGGAGC TGGCGGGCGG CGACCACAGG 
CCCTATACCT TCTTCATTGC CCCAGGGACC AGAGACTCAG GTGGGACTTA TTACCTGAAT 
CTGACCAGCC GCTTCCACTG GTCAGCACTG GAAGTGAGCG TGGGCCTGTA CACGTCCCTG 
TGTCAGTACT TCAGTGAGAA GGAGATGATG TGGAAGACAG AGGGGCTTGT GCCTCTGGAG 
GAGACGTCCC CCAGCCAGGC CGTCTGCCTT ACCCGCCACC TCACTGCCTT TGGTGCCAGC 
CTCTTTGTCC CCCCCAGCCA CGTCCACTTC ATCTTTCCAG AGCCGGCCTC AGGCGTGAAC 
TACATCGTCC TGCTGACGTG TATCGTGTGC CTGGTCACCT ACACAGTCAT GGCCGTGATC 
CTGCGTAAGC TGGACCAGCT GGACGTCAGC CGGGTGCGCA TCATCCCTTT CTGCGGGAAG 
GGAGGCCTCT TCAAGTATGA GATCCTGGTC AAGACCGGCT GGGGCCGAGG CTCAGGTACC 
ACGGCACACG TGGGCATCAT GCTGTACGGG GCGGAGGGCC GCAGCGGCCA CCGGCACCTG 
GATGGGGACA GAGCCTTCCG CCGCAACAGC CTGGACGTGT TCCAGATCGC CACCCCGCAC 
AGCCTGGGCA GCGTGCGCAA GATCCGCGTG TGGCACGACA ACAAAGGCCT CAGCCCCGCC 
TGGTTCCTGC AGCACATCAT TGTCAGGGAC CTGCAGAACG CCCGCAGCAC CTTCTTCCTG 
GTCAATGACT GGCTGTCGGT GGAGACTGAG GCCAACGGCG GCCTCGTAGA GAAGGAGGTG 
CTGGCAGCAA GCGAGGCGGC CCTGCGGCGG TCCCGGCGCC TCCTCGTGGC CGAGCTGCAG 
CGCGGCTTCT TTGACAAGCA CATCTGGCTC TCCATATGGG ACCGGCCGCC TCGGAGCCGC 
TTCACTCGTG TCCAGCGGGC CACATGCTGC ATCCTCCTCG TCTGCCTCTT CCTGGGCGCC 
AATGCTGTGT GGTACGGGGT CGTGGGAGAC ACCGCCTCCA GCGCGGGGCC TGTGTCCACT 
CTCATCCCGT TGAGTGTTGA CACGATCGCC GTCGGCCTGG TGTCCAGTGT GGTCGTCTAT 
CCCGTCTACC TGGTCATCCT GTTTCTCTTC CGGATGTCCC GGAGCAAGGT GGCCGGGGCC 
CCGAGCCCCA CCCCTTCAGG GCAGCAGGTA CTGGACGTTG ACAGCTACCT GGACTCGTCT 
GTGCTGGATA GCTCCTTCCT CACCTTCCCG GGGCTGCGCA CAGAGGCCTT TGCTGGACAA 
ATGAAAAGCG ACTTATTTTT GGATGATTCT AAAAGCCTGG TGTGCTGGCC ATCCAGTGAA 
GGCACACTTA ACTGGCCGGA CCTGCTGAGT GACCCGTCCA TCATGGGCAG CACCCTGCAG 
CGGCTGGCTC GGGGCCGCAC GGGCCACACG CTGGGCCCAG AGGACGACGG TCTCTCCTTG 
CTGAGCCCCT CTTCACCTGC CAAATACTTC TCAGCTTCAG ATGAGGACCT GATCCAGCAG 
ATCCTGGCCG AAGGCGCCAG CAGCCTGGCC CCCACCCAGG ACACCCAAGT GGAAACGGAT 
CTGCTCACCA GCCTGTCTAG CGCCCCCGGG GAGACAACGG AAACGCTGAC GCTGCAGAGG 
CTGGGGGAGA GGGGGCCGCC GTGCAGCTCC GGCGTGACCT GGGAGCAGCC CACGTCGGCC 
AGGCTCTCCG GGACAGGGCT GGCGGACGGT CTGCAGAAGC GGCTGCTGCC CGCCTGGTGT 
GCGTCCCTGG CCCACGGGCT CAGCTTGCTC TTGGCTGCTG TGGCTGTGGG GGTCTCTGGC 
TGGGTAGGCT CCAGCTTCCC CCCTGGTGTG AGCGTGATGT GGCTCCTCTC AAGCAGCTCC 
AGTTTCTTGG CCTCCTTCCT TGGCTGGGAG CCCCTCAAGG TCCTGCTGGA AGCCCTGTAC 
TTCTCGCTGG TGGCCAAGCG GCTCCATCCT GATGAGGATG ATACCCTGGT GGAGAGCCCG 
GCTGTGACCC CTGTGAGCGA GCGTGTGCCT CGCGTGAGGC CCCCCCACGG CTTTGCGCTC 
TTCCTGGCAA AGGAGGAAGC CCGAAAGGTC AAGAGGCTGC ATGGGATGCT GAGGAGCCTG 
CTGGTGTATA TGCTCTTTTT GCTGGTGACG CTGTTGGCCA ACCACGGGGA TGCCTCATGT 
CACAACCATG CCTACCGCTT ACAGAGTGCC ATCAAGCAGG AGCTGGGCAG CCAGGCCTTC 
CTGGCCATCA CCCGGTCTGA TGAGTTCTGG CCATGGATGT CACACGTGCT GCTTCCCTAC 
ATCCATGGGA ACCAGTCCGG CCCAGAGCTG GGGCCCCCAC GGCTGCGGCA GGTGCGGCTG 
CAGGAAGCTC TGTGCCCAGA CCCTCCCGGC GCCGCGGTGC ATGCGTGCTC AGCCGGCCAG 
GACGCCTTCA GCACCGGGGA CTACAGCATT GGCTGGGGTA GTGCTGCCCA GAACTGCTCC 
GAGACGTGGG CCTACTCTGC GCCAGACCTA CTGGGCATCT GGTCCTGGGG CTACTGCGCT 
GTGTACGACA GCGGGGGCTA TGTGCAGGAG CTAGGGCTGA GCCTGGAGGA GAGCCGCGCG 
CAGCTGGGCT TCCTGCAGCT GCATAACTGG ATCGACAACA GGAGCCGCGC CGTGTTCGTG 
GAGCTCACGC GCTACAGCCC AGCCGTGGGG CTGCACGCCG CCGTCACGCT GCGCCTCGAG 
TTCCCCGCTG CCGGGCACGC GGTGACCGCT GTCAGCGTGC GTCCCTTCCC GCTGCAGCGC 
CTGAGCACCG GCCTCTCCCT GCCGCTGCTC ACCTCGGTGA GCCTGCTGCT CTTCGCGCTG 
TACTTCTCCG TGGCCGAGGT GCGCACCTGG CGCAGGGAAG GGTGTAGACG TGCGGCGCAG 
CCCGGGGCGT GGGCGCGGTG GCTGCTGGTG GCACTGACCG CGGCCGCAGC GCTCGTGCGC 
CTGGCCCAGC TGGGTGTTGC CGACCGCCAG TGGGCCCGCT TCTTGCGCCG CCGTCCGCGC 
CGATTCACCA GCTTCGAGCA GGTGGCGCAG CTGAGCGCCG CGGCTCGCGG CCTGGCCGCC 
TCGCTGCTCT TCCTGCTCCT GGTCAAGGCC GCGCAGCAGC TGCGCTTTGT GCGCCAGTGG 
TCGGTTTTTG GCAAGACGCT GTGCCGGGCC CTGCCGGAGC TCGTGGGGGC AGCCCTCGGC 
CTGGTGGCGC TCGCCGTGGC CTACGCGCAC CTGGCCGTTC TGCTGGTCTC CTCCTGTGTG 
GATTCATTCC GGAGTGCGGC GCGGGCCCTC TTGGTGCTGT GCCCTGGGTC AGGGGGTCCC 
GCCCTGTGCC CTGCCGAGTC CTGGCGCCTG TCCCCGCTGC TGTGCACCGG GCTCTGGGCC 
CTGCGCCTTT GGGGCGCCCT GCGGCTGGGG GCCGTCCTTC TGAGATGGCG GTACCATGCC 
CTGCGTGGGG AACTCTACCG ACCGGCCTGG GAGCCGCAGG ACTACGAGAT GGTGGAGCTC 
TTTCTGCGCA GGCTGCGCCT CTGGATGGGT TTCAGCAAGG TCAAGGAGTT CCGCCACAAA 
GTCCGCTTTG AAGGGATGGA GCCGCTGCCC TCCCGCTCCT CCAGGGGCTC CAAGTCCTCC 
CCGGATGCAC CCCCACCCAG CGGGGGCTCG GACACCTCAC GGCCCTCCAC GTCCTCCAGC 
CAGCTGGACA CGCTGAGTGG AGGCCTGGGC CGCCTGGGGC CCCGGGGGGA GCCGGAGCCC 
TCTCGCCTGC AAGCGGTGTT CGAGGCCCTC CTGACCCAGT TCGACCGACT CAACCAGGCC 
ACAGAGGATG TGTACCAGCT GGAGCAGAGG CTGCAGAGCC TGCGGGGCCG CAGGAGCACC 
GTTCCCCCAG CCTCGCCTCC CCACAGCCCC TGCTCAGCCC TGCAGCCGGC CCTGCCCAGC 
CGTCTTGCCC GGGCCAGTCG AGGCATGGGC CTGGCATCCG GCCCCAGCAG GGCTTCCCTG 
CGGGCTAAGA ACAAGGTCCA CCCCAGCAGC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide methylation profiling of ADPKD identified epigenetically regulated genes associated with renal cyst development.
Human genetics133(3)281-97(2014 Mar)
Woo YM,Bae JB,Oh YH,Lee YG,Lee MJ,Park EY,Choi JK,Lee S,Shin Y,Lyu J,Jung HY,Lee YS,Hwang YH,Kim YJ,Park JH

Hyperphosphorylation of polycystin-2 at a critical residue in disease reveals an essential role for polycystin-1-regulated dephosphorylation.
Human molecular genetics22(10)1924-39(2013 May)
Streets AJ,Wessely O,Peters DJ,Ong AC

A non-synonymous mutation in the canine Pkd1 gene is associated with autosomal dominant polycystic kidney disease in Bull Terriers.
PloS one6(7)e22455(2011)
Gharahkhani P,O'Leary CA,Kyaw-Tanner M,Sturm RA,Duffy DL

Analysis of the cytoplasmic interaction between polycystin-1 and polycystin-2.
American journal of physiology. Renal physiology297(5)F1310-5(2009 Nov)
Casuscelli J,Schmidt S,DeGray B,Petri ET,Celi? A,Folta-Stogniew E,Ehrlich BE,Boggon TJ

Linkage confirms canine pkd1 orthologue as a candidate for bull terrier polycystic kidney disease.
Animal genetics40(4)543-6(2009 Aug)
O'Leary CA,Duffy D,Biros I,Corley S

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PKD1 cDNA ORF clone, Canis lupus familiaris(dog)

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