ORF » Species Summary » Calidris pugnax » USP53 cDNA ORF clone

USP53 cDNA ORF clone, Calidris pugnax(ruff)

The following USP53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa70884 XM_014949935.1
Latest version!		 Calidris pugnax ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa70885 XM_014949936.1
Latest version!		 Calidris pugnax ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa70886 XM_014949937.1
Latest version!		 Calidris pugnax ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    USP53 ( XM_014949935.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014805421.1 Calidris pugnax ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OCa70884
    Clone ID Related Accession (Same CDS sequence) XM_014949935.1
    Accession Version XM_014949935.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCATGGG TGAAATTCTT GAGAAAACCT GGGGGTAACC TTGGAAAAGT TTATCAACCT 
    GGAAGTATAT TGTCCCTGGC CCCTACAAAA GGCTTATTAA ATGAACCCGG ACAAAACAGC 
    TGCTTTCTTA ATAGTGCTGT TCAGGTATTA TGGCAACTTG ACATATTTCG ACGAAGTTTA 
    AGAGGTTTAA CTGGACATGT GTGTCAGGGA GATGCGTGCA TATTTTGTGC TTTAAAGGCA 
    ATATTTTCAC AATTTCAACA CAGTCGAGAA AAAGCACTCC CGTCAGATAA TATGAGACAT 
    GCCCTGGCAG AAAGTTTTAA GGATGAACAG CGTTTCCAGC TTGGATTCAT GGATGATGCA 
    GCAGAATGCT TTGAAAACAT CCTTGAACGG ATTCATTTTC ACCTAGTGCC AAACAGCGAA 
    ACAGATATGT GCACTTCAAA ATCCTGTATT TCTCATCAGA AGTTTGCGAT GACACTGTAT 
    GAACAGTGTG TATGTCGCAG TTGTGGGGCA TCATCAGACC CGTTGCCTTT TACGGAATTT 
    GTGCGTTACA TTTCTACCAC AGCCCTCTGT AATGAAGTAG AAAAAATGAT GGAGAGGCAT 
    GAACGTCTCA AGCCAGAAAT GTTTGCAGAA TTGCTGCAGG CAGCAAATAC TGCTGATGAC 
    TACAGAAAGT GCCCTAGTAA CTGCGGTCAA AAAATAAAAA TTCGTCGTGT TCTTATGAAT 
    TGTCCTGAGA TTGTGACTAT CGGTTTAGTC TGGGATTCAG AACACTCTGA CCTGACAGAA 
    GAGGTTATGC GGAATCTGGC AACACAACTT TATCTCCCTG GGCTGTTCTA TAGGGTTACA 
    GATGAAAATG CCAAAAATAG TGAACTTTTT CTTGTGGGAA TGATTTGCTA CACAAGCCGA 
    CATTACTGTG CCTTTGCCTT TCATACCAAG AGTTGCAAAT GGGTGCTGTT TGATGATGCT 
    AATGTAAAAG AGATTGGAAC AAAATGGAAA GATGTGGTCT CCAGGTGCAT TCGATGTCAC 
    TTTCAGCCAT TGCTGCTATT TTATGCTAAC CCAGATGGCA CAGCCGTATC TCCAGAAGAT 
    GCTCCCAAGC AGATTATTCA TTGGTCTCAG TGCAAAGCAG GAGGAAATGG GGAGGACTTG 
    GGATTTGAAA AGCATTCTGC TCCTAAATCA GACCACATGA AAGAGAATGG AATTGGAGAC 
    CCTGCTAATC AAAGAAGTAA TAAAAAAGTT CAGCCGGATA ATTCAGCATT CAGTAGAAGC 
    CATATTCAAG CTAGTGGTGG TAGAGGTCCA GCTAAATTAG GTTACAGCGA TCAGAAGGAC 
    AGGCTAAAGG ACATATCCAG AGAGTGTGCT CAGAAAGCTG CTGATATGAA AAACTTCTCA 
    TCTTTACGGA AAGATGCAGA CAAAGGACCA AGAAAAGAAT CTGGGAGACA AAGAGATTTG 
    ATTGGGGAAG ATCGTTCCAG TGTTAAGTCA GGGTCTCCTC CAGTTGGAAA CGGACTTAGA 
    CACTGTGCTG ATCAGCGCAT ATACAGCAGC CAGGGAAAAG GTCCCTATAA GCATGACAAT 
    GCGCCTCACC AAGCAAAGCT TTCTGTACAG GTGCTTGGAT CAAATAAACC AGAAGCTTTT 
    CCTGCTGGGG AGAAACCAAC GGTCAGGACC CGGACTGATG GTGTCACTGG CTACGATACA 
    GACACCAGCC AAGACTCCAG GGACAAAGGA AGTATTATCA GCAGCAGAAG CAGAAGTAAA 
    GGTTGGAAAC CTATGCGAGA GACGCTAAAC GTAGACAGTA TTTTCAGTGA GACAGAGAAA 
    AAACAGCATA GCCCAAAGCA CAAGGCAAAT CCGAACAGTA AATCTAAGCA TGAAAAGGAG 
    CGGAGCTTTA ACCATTGGCC AAAAGAAAAC CAAATCCAGA AAGGTTTAAT GACTATATAT 
    GAAGATGAAA CGAAACAGGA TACAGGAAGT CGAAGCTCAC TGGATTCGGA GGGAAAAGGG 
    AATGCTGAAA AAAGCAAAGG ATTTACAGAG AGAAAAATCC TTGGTGACAG CTGGCAAATT 
    CAGAGGACAG AATCTGGATA TGAAAGCAGT GATCATATCA GCAATGGATC TGCTAATCCG 
    GACTCGCCTG TTATTGAAGG AATCAATTCA GCAGATGTCA AGAATGTTAA AGAAAGTGCT 
    TCCTGCAGGA GGAAGGAGTT GCCAGGGTGT TTGTCAGCTC CATGTCTGGA TTGCGAACAG 
    AACTTGTCAA CCAAAAAAGC TGACAGTGTG TTACACTCAG TATCCCAGCA GAACAGAAAC 
    TTTTATGACT TGAGGAAAGA CCAGATCAAT TCAGAAGTTA ACTACAAACC CCATGTTAAT 
    TTCCCGACAG AACTACATTT GCAGGTGCCG AGTCCTCCAG TAAAGAGAGC TGAAATGCCT 
    GAGACCAACA GGAAATTTTT CCCATCATCA GCTCTACAGC CAGTTCTTAA AGACATTGTT 
    AAGTCAGAAA GTAGGAACAA GGCAAATGAA CAGAATTCTT CAGAGTGGCT CAATCCAGAC 
    AAGTTTGAGA AGATGAACGT GTCAGCATAT TGTCCTGATG GCATAACCCA CCCTTACACA 
    GAGAACGTCT GTGGAAGGAA GCTGATCAAC AATGACTTTT ACTCCTCTTC CCAGCTGCAG 
    TCTCATCATG CAAGAACGTT TGTTCCCAAG GTGGGGGTGT CACCTTGTGT GCCACAGAAC 
    CTGTCAGAAA GGCCCGTGGT GCTTCCCCCT CCCGGTGAGC ATGCTGGGCA TCCGCTCCGA 
    AGGGTGGATG CTCTTTGGGT GCCTGAAGCA GTGTACCAGA ATCTTCCTCC CCCTTTACCA 
    CCCAAGAAAT ACGCTCTGTA TACTTTGCCA GGATCCGAAA ATAACTCGGC AGCTGATGTA 
    AAATCAGTAG AAGTGTTGCA AAATAATCCG TTAAGGCAAA CAGGTACACC TTTAAAATCT 
    GGATCAGAAG CAAGCGTATT TACAAATGAA CAAAGGCTTA AAGAGCCATT TCAAGGGAAT 
    GAACTGTTCG TTCATAAACC GGATTCTCCA CCTCGTTTAT CAGTTAATGA CTTTGGGACT 
    GTGTCTGAAA ACTTCTTAAA GAAAGGATCT CGTCATATGG GACCAAGTGC TGGCTATGTG 
    GATGGACATG ACAGTGTATC CCTAACAACT TATTTTTCGG TGGATAGCTG CATGACTGAC 
    ACGTACAGGA TGAAATATCA TCAGAGACCC AAGCTGTATT TTACAGACAG TGGAAGCTTT 
    CACAAAGAAA AGCATCCTGC ATCAGCTGGA GTAGAACTGA ATGCTACGTG CCATGCTACT 
    ATTGAGCCCA GGCATCCCAC GTCCGAGCAA AGGTACAAGG CCAATGTAGA AGGCATACAC 
    TGCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805421.1
    CDS656..4027
    Translation

    Target ORF information:

    RefSeq Version XM_014949935.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014949935.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCATGGG TGAAATTCTT GAGAAAACCT GGGGGTAACC TTGGAAAAGT TTATCAACCT 
    GGAAGTATAT TGTCCCTGGC CCCTACAAAA GGCTTATTAA ATGAACCCGG ACAAAACAGC 
    TGCTTTCTTA ATAGTGCTGT TCAGGTATTA TGGCAACTTG ACATATTTCG ACGAAGTTTA 
    AGAGGTTTAA CTGGACATGT GTGTCAGGGA GATGCGTGCA TATTTTGTGC TTTAAAGGCA 
    ATATTTTCAC AATTTCAACA CAGTCGAGAA AAAGCACTCC CGTCAGATAA TATGAGACAT 
    GCCCTGGCAG AAAGTTTTAA GGATGAACAG CGTTTCCAGC TTGGATTCAT GGATGATGCA 
    GCAGAATGCT TTGAAAACAT CCTTGAACGG ATTCATTTTC ACCTAGTGCC AAACAGCGAA 
    ACAGATATGT GCACTTCAAA ATCCTGTATT TCTCATCAGA AGTTTGCGAT GACACTGTAT 
    GAACAGTGTG TATGTCGCAG TTGTGGGGCA TCATCAGACC CGTTGCCTTT TACGGAATTT 
    GTGCGTTACA TTTCTACCAC AGCCCTCTGT AATGAAGTAG AAAAAATGAT GGAGAGGCAT 
    GAACGTCTCA AGCCAGAAAT GTTTGCAGAA TTGCTGCAGG CAGCAAATAC TGCTGATGAC 
    TACAGAAAGT GCCCTAGTAA CTGCGGTCAA AAAATAAAAA TTCGTCGTGT TCTTATGAAT 
    TGTCCTGAGA TTGTGACTAT CGGTTTAGTC TGGGATTCAG AACACTCTGA CCTGACAGAA 
    GAGGTTATGC GGAATCTGGC AACACAACTT TATCTCCCTG GGCTGTTCTA TAGGGTTACA 
    GATGAAAATG CCAAAAATAG TGAACTTTTT CTTGTGGGAA TGATTTGCTA CACAAGCCGA 
    CATTACTGTG CCTTTGCCTT TCATACCAAG AGTTGCAAAT GGGTGCTGTT TGATGATGCT 
    AATGTAAAAG AGATTGGAAC AAAATGGAAA GATGTGGTCT CCAGGTGCAT TCGATGTCAC 
    TTTCAGCCAT TGCTGCTATT TTATGCTAAC CCAGATGGCA CAGCCGTATC TCCAGAAGAT 
    GCTCCCAAGC AGATTATTCA TTGGTCTCAG TGCAAAGCAG GAGGAAATGG GGAGGACTTG 
    GGATTTGAAA AGCATTCTGC TCCTAAATCA GACCACATGA AAGAGAATGG AATTGGAGAC 
    CCTGCTAATC AAAGAAGTAA TAAAAAAGTT CAGCCGGATA ATTCAGCATT CAGTAGAAGC 
    CATATTCAAG CTAGTGGTGG TAGAGGTCCA GCTAAATTAG GTTACAGCGA TCAGAAGGAC 
    AGGCTAAAGG ACATATCCAG AGAGTGTGCT CAGAAAGCTG CTGATATGAA AAACTTCTCA 
    TCTTTACGGA AAGATGCAGA CAAAGGACCA AGAAAAGAAT CTGGGAGACA AAGAGATTTG 
    ATTGGGGAAG ATCGTTCCAG TGTTAAGTCA GGGTCTCCTC CAGTTGGAAA CGGACTTAGA 
    CACTGTGCTG ATCAGCGCAT ATACAGCAGC CAGGGAAAAG GTCCCTATAA GCATGACAAT 
    GCGCCTCACC AAGCAAAGCT TTCTGTACAG GTGCTTGGAT CAAATAAACC AGAAGCTTTT 
    CCTGCTGGGG AGAAACCAAC GGTCAGGACC CGGACTGATG GTGTCACTGG CTACGATACA 
    GACACCAGCC AAGACTCCAG GGACAAAGGA AGTATTATCA GCAGCAGAAG CAGAAGTAAA 
    GGTTGGAAAC CTATGCGAGA GACGCTAAAC GTAGACAGTA TTTTCAGTGA GACAGAGAAA 
    AAACAGCATA GCCCAAAGCA CAAGGCAAAT CCGAACAGTA AATCTAAGCA TGAAAAGGAG 
    CGGAGCTTTA ACCATTGGCC AAAAGAAAAC CAAATCCAGA AAGGTTTAAT GACTATATAT 
    GAAGATGAAA CGAAACAGGA TACAGGAAGT CGAAGCTCAC TGGATTCGGA GGGAAAAGGG 
    AATGCTGAAA AAAGCAAAGG ATTTACAGAG AGAAAAATCC TTGGTGACAG CTGGCAAATT 
    CAGAGGACAG AATCTGGATA TGAAAGCAGT GATCATATCA GCAATGGATC TGCTAATCCG 
    GACTCGCCTG TTATTGAAGG AATCAATTCA GCAGATGTCA AGAATGTTAA AGAAAGTGCT 
    TCCTGCAGGA GGAAGGAGTT GCCAGGGTGT TTGTCAGCTC CATGTCTGGA TTGCGAACAG 
    AACTTGTCAA CCAAAAAAGC TGACAGTGTG TTACACTCAG TATCCCAGCA GAACAGAAAC 
    TTTTATGACT TGAGGAAAGA CCAGATCAAT TCAGAAGTTA ACTACAAACC CCATGTTAAT 
    TTCCCGACAG AACTACATTT GCAGGTGCCG AGTCCTCCAG TAAAGAGAGC TGAAATGCCT 
    GAGACCAACA GGAAATTTTT CCCATCATCA GCTCTACAGC CAGTTCTTAA AGACATTGTT 
    AAGTCAGAAA GTAGGAACAA GGCAAATGAA CAGAATTCTT CAGAGTGGCT CAATCCAGAC 
    AAGTTTGAGA AGATGAACGT GTCAGCATAT TGTCCTGATG GCATAACCCA CCCTTACACA 
    GAGAACGTCT GTGGAAGGAA GCTGATCAAC AATGACTTTT ACTCCTCTTC CCAGCTGCAG 
    TCTCATCATG CAAGAACGTT TGTTCCCAAG GTGGGGGTGT CACCTTGTGT GCCACAGAAC 
    CTGTCAGAAA GGCCCGTGGT GCTTCCCCCT CCCGGTGAGC ATGCTGGGCA TCCGCTCCGA 
    AGGGTGGATG CTCTTTGGGT GCCTGAAGCA GTGTACCAGA ATCTTCCTCC CCCTTTACCA 
    CCCAAGAAAT ACGCTCTGTA TACTTTGCCA GGATCCGAAA ATAACTCGGC AGCTGATGTA 
    AAATCAGTAG AAGTGTTGCA AAATAATCCG TTAAGGCAAA CAGGTACACC TTTAAAATCT 
    GGATCAGAAG CAAGCGTATT TACAAATGAA CAAAGGCTTA AAGAGCCATT TCAAGGGAAT 
    GAACTGTTCG TTCATAAACC GGATTCTCCA CCTCGTTTAT CAGTTAATGA CTTTGGGACT 
    GTGTCTGAAA ACTTCTTAAA GAAAGGATCT CGTCATATGG GACCAAGTGC TGGCTATGTG 
    GATGGACATG ACAGTGTATC CCTAACAACT TATTTTTCGG TGGATAGCTG CATGACTGAC 
    ACGTACAGGA TGAAATATCA TCAGAGACCC AAGCTGTATT TTACAGACAG TGGAAGCTTT 
    CACAAAGAAA AGCATCCTGC ATCAGCTGGA GTAGAACTGA ATGCTACGTG CCATGCTACT 
    ATTGAGCCCA GGCATCCCAC GTCCGAGCAA AGGTACAAGG CCAATGTAGA AGGCATACAC 
    TGCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    USP53 ( XM_014949936.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014805422.1 Calidris pugnax ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OCa70885
    Clone ID Related Accession (Same CDS sequence) XM_014949936.1
    Accession Version XM_014949936.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCATGGG TGAAATTCTT GAGAAAACCT GGGGGTAACC TTGGAAAAGT TTATCAACCT 
    GGAAGTATAT TGTCCCTGGC CCCTACAAAA GGCTTATTAA ATGAACCCGG ACAAAACAGC 
    TGCTTTCTTA ATAGTGCTGT TCAGGTATTA TGGCAACTTG ACATATTTCG ACGAAGTTTA 
    AGAGGTTTAA CTGGACATGT GTGTCAGGGA GATGCGTGCA TATTTTGTGC TTTAAAGGCA 
    ATATTTTCAC AATTTCAACA CAGTCGAGAA AAAGCACTCC CGTCAGATAA TATGAGACAT 
    GCCCTGGCAG AAAGTTTTAA GGATGAACAG CGTTTCCAGC TTGGATTCAT GGATGATGCA 
    GCAGAATGCT TTGAAAACAT CCTTGAACGG ATTCATTTTC ACCTAGTGCC AAACAGCGAA 
    ACAGATATGT GCACTTCAAA ATCCTGTATT TCTCATCAGA AGTTTGCGAT GACACTGTAT 
    GAACAGTGTG TATGTCGCAG TTGTGGGGCA TCATCAGACC CGTTGCCTTT TACGGAATTT 
    GTGCGTTACA TTTCTACCAC AGCCCTCTGT AATGAAGTAG AAAAAATGAT GGAGAGGCAT 
    GAACGTCTCA AGCCAGAAAT GTTTGCAGAA TTGCTGCAGG CAGCAAATAC TGCTGATGAC 
    TACAGAAAGT GCCCTAGTAA CTGCGGTCAA AAAATAAAAA TTCGTCGTGT TCTTATGAAT 
    TGTCCTGAGA TTGTGACTAT CGGTTTAGTC TGGGATTCAG AACACTCTGA CCTGACAGAA 
    GAGGTTATGC GGAATCTGGC AACACAACTT TATCTCCCTG GGCTGTTCTA TAGGGTTACA 
    GATGAAAATG CCAAAAATAG TGAACTTTTT CTTGTGGGAA TGATTTGCTA CACAAGCCGA 
    CATTACTGTG CCTTTGCCTT TCATACCAAG AGTTGCAAAT GGGTGCTGTT TGATGATGCT 
    AATGTAAAAG AGATTGGAAC AAAATGGAAA GATGTGGTCT CCAGGTGCAT TCGATGTCAC 
    TTTCAGCCAT TGCTGCTATT TTATGCTAAC CCAGATGGCA CAGCCGTATC TCCAGAAGAT 
    GCTCCCAAGC AGATTATTCA TTGGTCTCAG TGCAAAGCAG GAGGAAATGG GGAGGACTTG 
    GGATTTGAAA AGCATTCTGC TCCTAAATCA GACCACATGA AAGAGAATGG AATTGGAGAC 
    CCTGCTAATC AAAGAAGTAA TAAAAAAGTT CAGCCGGATA ATTCAGCATT CAGTAGAAGC 
    CATATTCAAG CTAGTGGTGG TAGAGGTCCA GCTAAATTAG GTTACAGCGA TCAGAAGGAC 
    AGGCTAAAGG ACATATCCAG AGAGTGTGCT CAGAAAGCTG CTGATATGAA AAACTTCTCA 
    TCTTTACGGA AAGATGCAGA CAAAGGACCA AGAAAAGAAT CTGGGAGACA AAGAGATTTG 
    ATTGGGGAAG ATCGTTCCAG TGTTAAGTCA GGGTCTCCTC CAGTTGGAAA CGGACTTAGA 
    CACTGTGCTG ATCAGCGCAT ATACAGCAGC CAGGGAAAAG GTCCCTATAA GCATGACAAT 
    GCGCCTCACC AAGCAAAGCT TTCTGTACAG GTGCTTGGAT CAAATAAACC AGAAGCTTTT 
    CCTGCTGGGG AGAAACCAAC GGTCAGGACC CGGACTGATG GTGTCACTGG CTACGATACA 
    GACACCAGCC AAGACTCCAG GGACAAAGGA AGTATTATCA GCAGCAGAAG CAGAAGTAAA 
    GGTTGGAAAC CTATGCGAGA GACGCTAAAC GTAGACAGTA TTTTCAGTGA GACAGAGAAA 
    AAACAGCATA GCCCAAAGCA CAAGGCAAAT CCGAACAGTA AATCTAAGCA TGAAAAGGAG 
    CGGAGCTTTA ACCATTGGCC AAAAGAAAAC CAAATCCAGA AAGGTTTAAT GACTATATAT 
    GAAGATGAAA CGAAACAGGA TACAGGAAGT CGAAGCTCAC TGGATTCGGA GGGAAAAGGG 
    AATGCTGAAA AAAGCAAAGG ATTTACAGAG AGAAAAATCC TTGGTGACAG CTGGCAAATT 
    CAGAGGACAG AATCTGGATA TGAAAGCAGT GATCATATCA GCAATGGATC TGCTAATCCG 
    GACTCGCCTG TTATTGAAGG AATCAATTCA GCAGATGTCA AGAATGTTAA AGAAAGTGCT 
    TCCTGCAGGA AGGAGTTGCC AGGGTGTTTG TCAGCTCCAT GTCTGGATTG CGAACAGAAC 
    TTGTCAACCA AAAAAGCTGA CAGTGTGTTA CACTCAGTAT CCCAGCAGAA CAGAAACTTT 
    TATGACTTGA GGAAAGACCA GATCAATTCA GAAGTTAACT ACAAACCCCA TGTTAATTTC 
    CCGACAGAAC TACATTTGCA GGTGCCGAGT CCTCCAGTAA AGAGAGCTGA AATGCCTGAG 
    ACCAACAGGA AATTTTTCCC ATCATCAGCT CTACAGCCAG TTCTTAAAGA CATTGTTAAG 
    TCAGAAAGTA GGAACAAGGC AAATGAACAG AATTCTTCAG AGTGGCTCAA TCCAGACAAG 
    TTTGAGAAGA TGAACGTGTC AGCATATTGT CCTGATGGCA TAACCCACCC TTACACAGAG 
    AACGTCTGTG GAAGGAAGCT GATCAACAAT GACTTTTACT CCTCTTCCCA GCTGCAGTCT 
    CATCATGCAA GAACGTTTGT TCCCAAGGTG GGGGTGTCAC CTTGTGTGCC ACAGAACCTG 
    TCAGAAAGGC CCGTGGTGCT TCCCCCTCCC GGTGAGCATG CTGGGCATCC GCTCCGAAGG 
    GTGGATGCTC TTTGGGTGCC TGAAGCAGTG TACCAGAATC TTCCTCCCCC TTTACCACCC 
    AAGAAATACG CTCTGTATAC TTTGCCAGGA TCCGAAAATA ACTCGGCAGC TGATGTAAAA 
    TCAGTAGAAG TGTTGCAAAA TAATCCGTTA AGGCAAACAG GTACACCTTT AAAATCTGGA 
    TCAGAAGCAA GCGTATTTAC AAATGAACAA AGGCTTAAAG AGCCATTTCA AGGGAATGAA 
    CTGTTCGTTC ATAAACCGGA TTCTCCACCT CGTTTATCAG TTAATGACTT TGGGACTGTG 
    TCTGAAAACT TCTTAAAGAA AGGATCTCGT CATATGGGAC CAAGTGCTGG CTATGTGGAT 
    GGACATGACA GTGTATCCCT AACAACTTAT TTTTCGGTGG ATAGCTGCAT GACTGACACG 
    TACAGGATGA AATATCATCA GAGACCCAAG CTGTATTTTA CAGACAGTGG AAGCTTTCAC 
    AAAGAAAAGC ATCCTGCATC AGCTGGAGTA GAACTGAATG CTACGTGCCA TGCTACTATT 
    GAGCCCAGGC ATCCCACGTC CGAGCAAAGG TACAAGGCCA ATGTAGAAGG CATACACTGC 
    AGCAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805422.1
    CDS656..4024
    Translation

    Target ORF information:

    RefSeq Version XM_014949936.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014949936.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCATGGG TGAAATTCTT GAGAAAACCT GGGGGTAACC TTGGAAAAGT TTATCAACCT 
    GGAAGTATAT TGTCCCTGGC CCCTACAAAA GGCTTATTAA ATGAACCCGG ACAAAACAGC 
    TGCTTTCTTA ATAGTGCTGT TCAGGTATTA TGGCAACTTG ACATATTTCG ACGAAGTTTA 
    AGAGGTTTAA CTGGACATGT GTGTCAGGGA GATGCGTGCA TATTTTGTGC TTTAAAGGCA 
    ATATTTTCAC AATTTCAACA CAGTCGAGAA AAAGCACTCC CGTCAGATAA TATGAGACAT 
    GCCCTGGCAG AAAGTTTTAA GGATGAACAG CGTTTCCAGC TTGGATTCAT GGATGATGCA 
    GCAGAATGCT TTGAAAACAT CCTTGAACGG ATTCATTTTC ACCTAGTGCC AAACAGCGAA 
    ACAGATATGT GCACTTCAAA ATCCTGTATT TCTCATCAGA AGTTTGCGAT GACACTGTAT 
    GAACAGTGTG TATGTCGCAG TTGTGGGGCA TCATCAGACC CGTTGCCTTT TACGGAATTT 
    GTGCGTTACA TTTCTACCAC AGCCCTCTGT AATGAAGTAG AAAAAATGAT GGAGAGGCAT 
    GAACGTCTCA AGCCAGAAAT GTTTGCAGAA TTGCTGCAGG CAGCAAATAC TGCTGATGAC 
    TACAGAAAGT GCCCTAGTAA CTGCGGTCAA AAAATAAAAA TTCGTCGTGT TCTTATGAAT 
    TGTCCTGAGA TTGTGACTAT CGGTTTAGTC TGGGATTCAG AACACTCTGA CCTGACAGAA 
    GAGGTTATGC GGAATCTGGC AACACAACTT TATCTCCCTG GGCTGTTCTA TAGGGTTACA 
    GATGAAAATG CCAAAAATAG TGAACTTTTT CTTGTGGGAA TGATTTGCTA CACAAGCCGA 
    CATTACTGTG CCTTTGCCTT TCATACCAAG AGTTGCAAAT GGGTGCTGTT TGATGATGCT 
    AATGTAAAAG AGATTGGAAC AAAATGGAAA GATGTGGTCT CCAGGTGCAT TCGATGTCAC 
    TTTCAGCCAT TGCTGCTATT TTATGCTAAC CCAGATGGCA CAGCCGTATC TCCAGAAGAT 
    GCTCCCAAGC AGATTATTCA TTGGTCTCAG TGCAAAGCAG GAGGAAATGG GGAGGACTTG 
    GGATTTGAAA AGCATTCTGC TCCTAAATCA GACCACATGA AAGAGAATGG AATTGGAGAC 
    CCTGCTAATC AAAGAAGTAA TAAAAAAGTT CAGCCGGATA ATTCAGCATT CAGTAGAAGC 
    CATATTCAAG CTAGTGGTGG TAGAGGTCCA GCTAAATTAG GTTACAGCGA TCAGAAGGAC 
    AGGCTAAAGG ACATATCCAG AGAGTGTGCT CAGAAAGCTG CTGATATGAA AAACTTCTCA 
    TCTTTACGGA AAGATGCAGA CAAAGGACCA AGAAAAGAAT CTGGGAGACA AAGAGATTTG 
    ATTGGGGAAG ATCGTTCCAG TGTTAAGTCA GGGTCTCCTC CAGTTGGAAA CGGACTTAGA 
    CACTGTGCTG ATCAGCGCAT ATACAGCAGC CAGGGAAAAG GTCCCTATAA GCATGACAAT 
    GCGCCTCACC AAGCAAAGCT TTCTGTACAG GTGCTTGGAT CAAATAAACC AGAAGCTTTT 
    CCTGCTGGGG AGAAACCAAC GGTCAGGACC CGGACTGATG GTGTCACTGG CTACGATACA 
    GACACCAGCC AAGACTCCAG GGACAAAGGA AGTATTATCA GCAGCAGAAG CAGAAGTAAA 
    GGTTGGAAAC CTATGCGAGA GACGCTAAAC GTAGACAGTA TTTTCAGTGA GACAGAGAAA 
    AAACAGCATA GCCCAAAGCA CAAGGCAAAT CCGAACAGTA AATCTAAGCA TGAAAAGGAG 
    CGGAGCTTTA ACCATTGGCC AAAAGAAAAC CAAATCCAGA AAGGTTTAAT GACTATATAT 
    GAAGATGAAA CGAAACAGGA TACAGGAAGT CGAAGCTCAC TGGATTCGGA GGGAAAAGGG 
    AATGCTGAAA AAAGCAAAGG ATTTACAGAG AGAAAAATCC TTGGTGACAG CTGGCAAATT 
    CAGAGGACAG AATCTGGATA TGAAAGCAGT GATCATATCA GCAATGGATC TGCTAATCCG 
    GACTCGCCTG TTATTGAAGG AATCAATTCA GCAGATGTCA AGAATGTTAA AGAAAGTGCT 
    TCCTGCAGGA AGGAGTTGCC AGGGTGTTTG TCAGCTCCAT GTCTGGATTG CGAACAGAAC 
    TTGTCAACCA AAAAAGCTGA CAGTGTGTTA CACTCAGTAT CCCAGCAGAA CAGAAACTTT 
    TATGACTTGA GGAAAGACCA GATCAATTCA GAAGTTAACT ACAAACCCCA TGTTAATTTC 
    CCGACAGAAC TACATTTGCA GGTGCCGAGT CCTCCAGTAA AGAGAGCTGA AATGCCTGAG 
    ACCAACAGGA AATTTTTCCC ATCATCAGCT CTACAGCCAG TTCTTAAAGA CATTGTTAAG 
    TCAGAAAGTA GGAACAAGGC AAATGAACAG AATTCTTCAG AGTGGCTCAA TCCAGACAAG 
    TTTGAGAAGA TGAACGTGTC AGCATATTGT CCTGATGGCA TAACCCACCC TTACACAGAG 
    AACGTCTGTG GAAGGAAGCT GATCAACAAT GACTTTTACT CCTCTTCCCA GCTGCAGTCT 
    CATCATGCAA GAACGTTTGT TCCCAAGGTG GGGGTGTCAC CTTGTGTGCC ACAGAACCTG 
    TCAGAAAGGC CCGTGGTGCT TCCCCCTCCC GGTGAGCATG CTGGGCATCC GCTCCGAAGG 
    GTGGATGCTC TTTGGGTGCC TGAAGCAGTG TACCAGAATC TTCCTCCCCC TTTACCACCC 
    AAGAAATACG CTCTGTATAC TTTGCCAGGA TCCGAAAATA ACTCGGCAGC TGATGTAAAA 
    TCAGTAGAAG TGTTGCAAAA TAATCCGTTA AGGCAAACAG GTACACCTTT AAAATCTGGA 
    TCAGAAGCAA GCGTATTTAC AAATGAACAA AGGCTTAAAG AGCCATTTCA AGGGAATGAA 
    CTGTTCGTTC ATAAACCGGA TTCTCCACCT CGTTTATCAG TTAATGACTT TGGGACTGTG 
    TCTGAAAACT TCTTAAAGAA AGGATCTCGT CATATGGGAC CAAGTGCTGG CTATGTGGAT 
    GGACATGACA GTGTATCCCT AACAACTTAT TTTTCGGTGG ATAGCTGCAT GACTGACACG 
    TACAGGATGA AATATCATCA GAGACCCAAG CTGTATTTTA CAGACAGTGG AAGCTTTCAC 
    AAAGAAAAGC ATCCTGCATC AGCTGGAGTA GAACTGAATG CTACGTGCCA TGCTACTATT 
    GAGCCCAGGC ATCCCACGTC CGAGCAAAGG TACAAGGCCA ATGTAGAAGG CATACACTGC 
    AGCAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    USP53 ( XM_014949937.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014805423.1 Calidris pugnax ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OCa70886
    Clone ID Related Accession (Same CDS sequence) XM_014949937.1
    Accession Version XM_014949937.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090920.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCATGGG TGAAATTCTT GAGAAAACCT GGGGGTAACC TTGGAAAAGT TTATCAACCT 
    GGAAGTATAT TGTCCCTGGC CCCTACAAAA GGCTTATTAA ATGAACCCGG ACAAAACAGC 
    TGCTTTCTTA ATAGTGCTGT TCAGGTATTA TGGCAACTTG ACATATTTCG ACGAAGTTTA 
    AGAGGTTTAA CTGGACATGT GTGTCAGGGA GATGCGTGCA TATTTTGTGC TTTAAAGGCA 
    ATATTTTCAC AATTTCAACA CAGTCGAGAA AAAGCACTCC CGTCAGATAA TATGAGACAT 
    GCCCTGGCAG AAAGTTTTAA GGATGAACAG CGTTTCCAGC TTGGATTCAT GGATGATGCA 
    GCAGAATGCT TTGAAAACAT CCTTGAACGG ATTCATTTTC ACCTAGTGCC AAACAGCGAA 
    ACAGATATGT GCACTTCAAA ATCCTGTATT TCTCATCAGA AGTTTGCGAT GACACTGTAT 
    GAACAGTGTG TATGTCGCAG TTGTGGGGCA TCATCAGACC CGTTGCCTTT TACGGAATTT 
    GTGCGTTACA TTTCTACCAC AGCCCTCTGT AATGAAGTAG AAAAAATGAT GGAGAGGCAT 
    GAACGTCTCA AGCCAGAAAT GTTTGCAGAA TTGCTGCAGG CAGCAAATAC TGCTGATGAC 
    TACAGAAAGT GCCCTAGTAA CTGCGGTCAA AAAATAAAAA TTCGTCGTGT TCTTATGAAT 
    TGTCCTGAGA TTGTGACTAT CGGTTTAGTC TGGGATTCAG AACACTCTGA CCTGACAGAA 
    GAGGTTATGC GGAATCTGGC AACACAACTT TATCTCCCTG GGCTGTTCTA TAGGGTTACA 
    GATGAAAATG CCAAAAATAG TGAACTTTTT CTTGTGGGAA TGATTTGCTA CACAAGCCGA 
    CATTACTGTG CCTTTGCCTT TCATACCAAG AGTTGCAAAT GGGTGCTGTT TGATGATGCT 
    AATGTAAAAG AGATTGGAAC AAAATGGAAA GATGTGGTCT CCAGGTGCAT TCGATGTCAC 
    TTTCAGCCAT TGCTGCTATT TTATGCTAAC CCAGATGGCA CAGCCGTATC TCCAGAAGAT 
    GCTCCCAAGC AGATTATTCA TTGGTCTCAG TGCAAAGCAG GAGGAAATGG GGAGGACTTG 
    GGATTTGAAA AGCATTCTGC TCCTAAATCA GACCACATGA AAGAGAATGG AATTGGAGAC 
    CCTGCTAATC AAAGAAGTAA TAAAAAAGTT CAGCCGGATA ATTCAGCATT CAGTAGAAGC 
    CATATTCAAG CTAGTGGTGG TAGAGGTCCA GCTAAATTAG GTTACAGCGA TCAGAAGGAC 
    AGGCTAAAGG ACATATCCAG AGAGTGTGCT CAGAAAGCTG CTGATATGAA AAACTTCTCA 
    TCTTTACGGA AAGATGCAGA CAAAGGACCA AGAAAAGAAT CTGGGAGACA AAGAGATTTG 
    ATTGGGGAAG ATCGTTCCAG TGTTAAGTCA GGGTCTCCTC CAGTTGGAAA CGGACTTAGA 
    CACTGTGCTG ATCAGCGCAT ATACAGCAGC CAGGGAAAAG GTCCCTATAA GCATGACAAT 
    GCGCCTCACC AAGCAAAGCT TTCTGTACAG GTGCTTGGAT CAAATAAACC AGAAGCTTTT 
    CCTGCTGGGG AGAAACCAAC GGTCAGGACC CGGACTGATG GTGTCACTGG CTACGATACA 
    GACACCAGCC AAGACTCCAG GGACAAAGGA AGTATTATCA GCAGCAGAAG CAGAAGTAAA 
    GGTTGGAAAC CTATGCGAGA GACGCTAAAC GTAGACAGTA TTTTCAGTGA GACAGAGAAA 
    AAACAGCATA GCCCAAAGCA CAAGGCAAAT CCGAACAGTA AATCTAAGCA TGAAAAGGAG 
    CGGAGCTTTA ACCATTGGCC AAAAGAAAAC CAAATCCAGA AAGGTTTAAT GACTATATAT 
    GAAGATGAAA CGAAACAGGA TACAGGAAGT CGAAGCTCAC TGGATTCGGA GGGAAAAGGG 
    AATGCTGAAA AAAGCAAAGG ATTTACAGAG AGAAAAATCC TTGGTGACAG CTGGCAAATT 
    CAGAGGACAG AATCTGGATA TGAAAGCAGT GATCATATCA GCAATGGATC TGCTAATCCG 
    GACTCGCCTG TTATTGAAGG AATCAATTCA GCAGATGTCA AGAATGTTAA AGAAAGTGCT 
    TCCTGCAGCG AACAGAACTT GTCAACCAAA AAAGCTGACA GTGTGTTACA CTCAGTATCC 
    CAGCAGAACA GAAACTTTTA TGACTTGAGG AAAGACCAGA TCAATTCAGA AGTTAACTAC 
    AAACCCCATG TTAATTTCCC GACAGAACTA CATTTGCAGG TGCCGAGTCC TCCAGTAAAG 
    AGAGCTGAAA TGCCTGAGAC CAACAGGAAA TTTTTCCCAT CATCAGCTCT ACAGCCAGTT 
    CTTAAAGACA TTGTTAAGTC AGAAAGTAGG AACAAGGCAA ATGAACAGAA TTCTTCAGAG 
    TGGCTCAATC CAGACAAGTT TGAGAAGATG AACGTGTCAG CATATTGTCC TGATGGCATA 
    ACCCACCCTT ACACAGAGAA CGTCTGTGGA AGGAAGCTGA TCAACAATGA CTTTTACTCC 
    TCTTCCCAGC TGCAGTCTCA TCATGCAAGA ACGTTTGTTC CCAAGGTGGG GGTGTCACCT 
    TGTGTGCCAC AGAACCTGTC AGAAAGGCCC GTGGTGCTTC CCCCTCCCGG TGAGCATGCT 
    GGGCATCCGC TCCGAAGGGT GGATGCTCTT TGGGTGCCTG AAGCAGTGTA CCAGAATCTT 
    CCTCCCCCTT TACCACCCAA GAAATACGCT CTGTATACTT TGCCAGGATC CGAAAATAAC 
    TCGGCAGCTG ATGTAAAATC AGTAGAAGTG TTGCAAAATA ATCCGTTAAG GCAAACAGGT 
    ACACCTTTAA AATCTGGATC AGAAGCAAGC GTATTTACAA ATGAACAAAG GCTTAAAGAG 
    CCATTTCAAG GGAATGAACT GTTCGTTCAT AAACCGGATT CTCCACCTCG TTTATCAGTT 
    AATGACTTTG GGACTGTGTC TGAAAACTTC TTAAAGAAAG GATCTCGTCA TATGGGACCA 
    AGTGCTGGCT ATGTGGATGG ACATGACAGT GTATCCCTAA CAACTTATTT TTCGGTGGAT 
    AGCTGCATGA CTGACACGTA CAGGATGAAA TATCATCAGA GACCCAAGCT GTATTTTACA 
    GACAGTGGAA GCTTTCACAA AGAAAAGCAT CCTGCATCAG CTGGAGTAGA ACTGAATGCT 
    ACGTGCCATG CTACTATTGA GCCCAGGCAT CCCACGTCCG AGCAAAGGTA CAAGGCCAAT 
    GTAGAAGGCA TACACTGCAG CAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014805423.1
    CDS655..3981
    Translation

    Target ORF information:

    RefSeq Version XM_014949937.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014949937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCATGGG TGAAATTCTT GAGAAAACCT GGGGGTAACC TTGGAAAAGT TTATCAACCT 
    GGAAGTATAT TGTCCCTGGC CCCTACAAAA GGCTTATTAA ATGAACCCGG ACAAAACAGC 
    TGCTTTCTTA ATAGTGCTGT TCAGGTATTA TGGCAACTTG ACATATTTCG ACGAAGTTTA 
    AGAGGTTTAA CTGGACATGT GTGTCAGGGA GATGCGTGCA TATTTTGTGC TTTAAAGGCA 
    ATATTTTCAC AATTTCAACA CAGTCGAGAA AAAGCACTCC CGTCAGATAA TATGAGACAT 
    GCCCTGGCAG AAAGTTTTAA GGATGAACAG CGTTTCCAGC TTGGATTCAT GGATGATGCA 
    GCAGAATGCT TTGAAAACAT CCTTGAACGG ATTCATTTTC ACCTAGTGCC AAACAGCGAA 
    ACAGATATGT GCACTTCAAA ATCCTGTATT TCTCATCAGA AGTTTGCGAT GACACTGTAT 
    GAACAGTGTG TATGTCGCAG TTGTGGGGCA TCATCAGACC CGTTGCCTTT TACGGAATTT 
    GTGCGTTACA TTTCTACCAC AGCCCTCTGT AATGAAGTAG AAAAAATGAT GGAGAGGCAT 
    GAACGTCTCA AGCCAGAAAT GTTTGCAGAA TTGCTGCAGG CAGCAAATAC TGCTGATGAC 
    TACAGAAAGT GCCCTAGTAA CTGCGGTCAA AAAATAAAAA TTCGTCGTGT TCTTATGAAT 
    TGTCCTGAGA TTGTGACTAT CGGTTTAGTC TGGGATTCAG AACACTCTGA CCTGACAGAA 
    GAGGTTATGC GGAATCTGGC AACACAACTT TATCTCCCTG GGCTGTTCTA TAGGGTTACA 
    GATGAAAATG CCAAAAATAG TGAACTTTTT CTTGTGGGAA TGATTTGCTA CACAAGCCGA 
    CATTACTGTG CCTTTGCCTT TCATACCAAG AGTTGCAAAT GGGTGCTGTT TGATGATGCT 
    AATGTAAAAG AGATTGGAAC AAAATGGAAA GATGTGGTCT CCAGGTGCAT TCGATGTCAC 
    TTTCAGCCAT TGCTGCTATT TTATGCTAAC CCAGATGGCA CAGCCGTATC TCCAGAAGAT 
    GCTCCCAAGC AGATTATTCA TTGGTCTCAG TGCAAAGCAG GAGGAAATGG GGAGGACTTG 
    GGATTTGAAA AGCATTCTGC TCCTAAATCA GACCACATGA AAGAGAATGG AATTGGAGAC 
    CCTGCTAATC AAAGAAGTAA TAAAAAAGTT CAGCCGGATA ATTCAGCATT CAGTAGAAGC 
    CATATTCAAG CTAGTGGTGG TAGAGGTCCA GCTAAATTAG GTTACAGCGA TCAGAAGGAC 
    AGGCTAAAGG ACATATCCAG AGAGTGTGCT CAGAAAGCTG CTGATATGAA AAACTTCTCA 
    TCTTTACGGA AAGATGCAGA CAAAGGACCA AGAAAAGAAT CTGGGAGACA AAGAGATTTG 
    ATTGGGGAAG ATCGTTCCAG TGTTAAGTCA GGGTCTCCTC CAGTTGGAAA CGGACTTAGA 
    CACTGTGCTG ATCAGCGCAT ATACAGCAGC CAGGGAAAAG GTCCCTATAA GCATGACAAT 
    GCGCCTCACC AAGCAAAGCT TTCTGTACAG GTGCTTGGAT CAAATAAACC AGAAGCTTTT 
    CCTGCTGGGG AGAAACCAAC GGTCAGGACC CGGACTGATG GTGTCACTGG CTACGATACA 
    GACACCAGCC AAGACTCCAG GGACAAAGGA AGTATTATCA GCAGCAGAAG CAGAAGTAAA 
    GGTTGGAAAC CTATGCGAGA GACGCTAAAC GTAGACAGTA TTTTCAGTGA GACAGAGAAA 
    AAACAGCATA GCCCAAAGCA CAAGGCAAAT CCGAACAGTA AATCTAAGCA TGAAAAGGAG 
    CGGAGCTTTA ACCATTGGCC AAAAGAAAAC CAAATCCAGA AAGGTTTAAT GACTATATAT 
    GAAGATGAAA CGAAACAGGA TACAGGAAGT CGAAGCTCAC TGGATTCGGA GGGAAAAGGG 
    AATGCTGAAA AAAGCAAAGG ATTTACAGAG AGAAAAATCC TTGGTGACAG CTGGCAAATT 
    CAGAGGACAG AATCTGGATA TGAAAGCAGT GATCATATCA GCAATGGATC TGCTAATCCG 
    GACTCGCCTG TTATTGAAGG AATCAATTCA GCAGATGTCA AGAATGTTAA AGAAAGTGCT 
    TCCTGCAGCG AACAGAACTT GTCAACCAAA AAAGCTGACA GTGTGTTACA CTCAGTATCC 
    CAGCAGAACA GAAACTTTTA TGACTTGAGG AAAGACCAGA TCAATTCAGA AGTTAACTAC 
    AAACCCCATG TTAATTTCCC GACAGAACTA CATTTGCAGG TGCCGAGTCC TCCAGTAAAG 
    AGAGCTGAAA TGCCTGAGAC CAACAGGAAA TTTTTCCCAT CATCAGCTCT ACAGCCAGTT 
    CTTAAAGACA TTGTTAAGTC AGAAAGTAGG AACAAGGCAA ATGAACAGAA TTCTTCAGAG 
    TGGCTCAATC CAGACAAGTT TGAGAAGATG AACGTGTCAG CATATTGTCC TGATGGCATA 
    ACCCACCCTT ACACAGAGAA CGTCTGTGGA AGGAAGCTGA TCAACAATGA CTTTTACTCC 
    TCTTCCCAGC TGCAGTCTCA TCATGCAAGA ACGTTTGTTC CCAAGGTGGG GGTGTCACCT 
    TGTGTGCCAC AGAACCTGTC AGAAAGGCCC GTGGTGCTTC CCCCTCCCGG TGAGCATGCT 
    GGGCATCCGC TCCGAAGGGT GGATGCTCTT TGGGTGCCTG AAGCAGTGTA CCAGAATCTT 
    CCTCCCCCTT TACCACCCAA GAAATACGCT CTGTATACTT TGCCAGGATC CGAAAATAAC 
    TCGGCAGCTG ATGTAAAATC AGTAGAAGTG TTGCAAAATA ATCCGTTAAG GCAAACAGGT 
    ACACCTTTAA AATCTGGATC AGAAGCAAGC GTATTTACAA ATGAACAAAG GCTTAAAGAG 
    CCATTTCAAG GGAATGAACT GTTCGTTCAT AAACCGGATT CTCCACCTCG TTTATCAGTT 
    AATGACTTTG GGACTGTGTC TGAAAACTTC TTAAAGAAAG GATCTCGTCA TATGGGACCA 
    AGTGCTGGCT ATGTGGATGG ACATGACAGT GTATCCCTAA CAACTTATTT TTCGGTGGAT 
    AGCTGCATGA CTGACACGTA CAGGATGAAA TATCATCAGA GACCCAAGCT GTATTTTACA 
    GACAGTGGAA GCTTTCACAA AGAAAAGCAT CCTGCATCAG CTGGAGTAGA ACTGAATGCT 
    ACGTGCCATG CTACTATTGA GCCCAGGCAT CCCACGTCCG AGCAAAGGTA CAAGGCCAAT 
    GTAGAAGGCA TACACTGCAG CAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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