DHX34 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHX34
Entrez Gene ID	106893936
Full Name	DExH-box helicase 34
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014952289.1	XP_014807775.1	probable ATP-dependent RNA helicase DHX34

Pathways from BioSystems
Gene Ontology

The following DHX34 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX34 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa72543	XM_014952289.1 Latest version!	Calidris pugnax DEAH (Asp-Glu-Ala-His) box polypeptide 34 (DHX34), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHX34 ( XM_014952289.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807775.1 Calidris pugnax DEAH (Asp-Glu-Ala-His) box polypeptide 34 (DHX34), mRNA.

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CloneID	OCa72543
Clone ID Related Accession (Same CDS sequence)	XM_014952289.1
Accession Version	XM_014952289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	probable ATP-dependent RNA helicase DHX34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090959.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGGATGG AGGAGGAGGA GAGCGGCTCC CGGCCCCGAG GGGAGCGAGC CCGGTCGCCC 
CCCCAGTGGG ACTGGGACCA CCCGGCCACG CGCCGCCCCC TGGAAGAGCT CTATTTCCCC 
CGGCAGGATC CCGCCTCTTC CCAGGATATT CCCAATTTCT GGGCTTTTTT CCAGCGCCTC 
CGACGTTTCC AGAGCCGGAA CCCCCACCCC CCTGAGCCTT GCCCTGAGGT GGCCACCTCT 
GGCCCCCTGG ACTTGCCCCG TGGCTACCAC CCCCGGTACC GCATCAACCT CTCCCTTCCC 
AGGCCGGGGA GGGGGGGGGA TTGTGGCATC CCCCGGGATC GCCTGGCGGA ATTCCGCGCC 
GCTTTCCTCC ACTACCTGGA TTTCCCCCAA AAACAAAGCT TCGCCAAACT GGCCAAACTC 
CAACGGGAAC GAGCCGCGCT GCCCATCGCC CGGTACCGGG AGAAGCTGGT GGAGCTGGTG 
GCCCAGCACC GGGTGGTGGT GGTGGCCGGA GACACCGGCT GCGGCAAATC CACCCAGGTG 
CCCCAGTTCC TGCTGGCGGC CGGTTACAGC CACGTGGCCT GTAGCCAGCC CCGCCGCATC 
GCCTGCGTGG CGCTGGCCAA GAGGGTGGCC CTGGAGAGCC TGCACCAGTA CGGCAGCCAG 
GTGGGCTACC AGATCCGCTT CGAGAGCACG CGCTCCCCGG CCACCCGCCT GCTCTTCCTG 
ACGGAGGGGC TGCTCCTGCG TCAGGCCCAG CGGGCGCCCG CGCTGCCCGA GTACCGCGTC 
CTGGTGGCCG ACGAGGTCCA CGAGCGGCAC CTGCAGGGGG ACGTCCTGCT GGGCGTCCTG 
CGCCGCCTCC TCTCCGCCCG CCCCGACCTC CGCCTGGTCC TCATGTCGGC CACCATCAAC 
ATCCCCCTCT TCTCCGGCTA CTTCGGGGGG GCCCCGGTGC TGCAGGTCCC GGGGAGGATC 
TTCCCCATCT CGGTCATCTA CCAGCCCATC CCTAAGGAAG AGGCCCCGGC GGGTGGGAAA 
TGGGGGAAAG CGGAGCGCCT GGACCCTCTG CCCTACCTGC GGGTGCTTCA GGCCATCGAC 
CACAAGTACC CCCCGGAGGA GCGGGGGGAC CTACTGGTGT TCCTCAGCGG GGTGGCCGAG 
ATCGGGGCGG TGCAGGAGGC GGCCCAGGCT TATGCCACCC ACACCCAGCG CTGGATTGTC 
CTCCCCCTGC ACAGCACCCT CTCGGTGGCT GAGCAGGACA AGGTGTTTGA CGTCCCCCCT 
CCCGGCGTCC GTAAGTGCAT CCTCTCCACC AACATCGCCG AGACCTCGGT GACCATCGAC 
GGCGTCCGCT TTGTCCTGGA TTCGGGGAAG GTGAAGGAGA TGAGTTACGA CCCTCAGGGC 
AAACTCCAGC GGCTGCAGGA GTTCTGGATC AGCCGCGCCA GCGCCGAGCA GCGCAAGGGA 
CGCGCCGGCA GGACCGGCCC CGGCGTCTGC TACCGCCTCT ACGCCGAATC CGACTACGAC 
GCCTTCGCCC CTTACCCCGT GCCGGAGATC CAGCGGGTAG CGCTCGATTC GCTGGTGCTC 
CAGTTAAAGA GTATGGGGCT GGGCGACCCC CGGACCTTCC CTTTTCTGGA GCCCCCACCC 
TTGTCCAGCC TGGAGACAGC CCTGAGGTAC CTGCAGGACC AAGGAGCCCT GGATGAGGCC 
GAAGACCTGA CGCCCATCGG GAACCTGCTG GCCCAGCTGC CGGTAGATGT GGTGGTCGGC 
AAGATGCTGG TCCTGGGCAC CCTCTTTGGC CTGGCTGAGC CCACCCTGAC GGTGGCAGCG 
GCGCTGAGCG TCCCATCCCC CTTCCTGAGA CCCTCTCACC CCAACCCCGA GTGCGCCACG 
GCCCGGCGGC CCCTGGAGAG CCCCCACGGT GACCCCCTGA CTCTGCTCAA CCTCTTCAAC 
GAGTGGGTCC AGGTGAAATC GGAGCGGAGC GGCAGCTCTC GGAAGTGGTG CCGGAGGCGA 
GGTTTGGAGG AGCATCGTCT CTATGAAGCT GCCAACCTTC GGCGCCAGTT CCAGGAGCTT 
CTTCGAGACC ACCAGCTACT GGAGGAAGAT TCTGGTCGCC CCAGCGACAG CTACAGCCGG 
CAGAGCCGGC ACCGGGAACG CCGGGAACTG CACCGGCTCT GGCGATCCCA CACCGAGACC 
GAAGGCCGGA AGCGCAAGGT GCTGCGGCTG CAGGACGGAG CCGATGCCTC CTCCGGCGAG 
GAGGAGGATG ATGGTGGTAC CCGTGGCAAA GGGGAGCGCA GCATCGATAT CCAGGATGTC 
AAGTTCAAGC TCCGCCACAA CGTGGAGGAG CTCCAGGCCG TCTCCAGCTC GGCCCTCTCC 
TCCTCGCAGC TCGCCCTGCT CAAACTGGTG CTGTGCCGGG GGCTTTACCC CCAACTGGCC 
GTCCCTGACC AGTTCAACAG TGGCCGCAAA GACTCCGACC AGATTTTTCA CACCAAAAAC 
AAGCAAGGGG TCGTCCTTCA TCCCACCTGT GTCTTCGCCA CCAGCCCAGA GCTGCTCCAT 
GCCAAGGAGA GACGGGACCG TGGTGGGACT GGAGAGCCCA CAGAGGGGCT GAGCTGCCAC 
CACCAGCTCC TGGCCTTTGT CTCGCTGCTG GAGACCAACA AGCCCTACCT GGTGAACTGT 
GTCCGGGTGC CGGCCCTCCA GGCTCTCCTC CTCTTCTCCC GCTCGCTGGA CACCAGTGGG 
GACTGCGCCC GGCTGGTGGC CGATGGGTGG CTGGAGGTCA CCATCCCCGA CGCGGACTCT 
GCCCTGCGCC TGCTCTCCAC GGCCCTGCAG CTCCGTTCCT CCTGGGAAAA ACTCCTTCAT 
CAGCTGCTGG AAGGTCGAGG AGAGGAACTG GGCCACCATC CCAGCCCCCG TGACGTGTCC 
CTGCTCAGCC GGGGGCTACT GGACTTCCTG CAGATGGAGG TGCCATATCG TCTGCGCCAG 
CTCACGGGGC TGGAGAAACA GAACCTCTAT GTGGGTCCCC AAACGGTGGC GGCTGCTCCC 
CGGCTCCCAG GGCTCTTCCA GGGGGCAGAG ATGAAGCCTG ACGAGGTGAA AGGAGGCCAC 
AGGGTGACGG ATTTTCTCAC CTACAACTGT CTGGCCACGG ACACGGATCT CTACAGCGAC 
TGTCTGAGGA GCTTCTGGAC CTGTCCCCAC TGCGACCTCC ACATGCCCTT CACTCCCCTG 
GAGCGCATGT GCCACCAAAG CGCTTGCCGG CCCCCTGAGG CCCCCCTGGA AGAACCCTCA 
GAAAACTCCT CCAAAACCTC TGCCCTGCAC CGATCCTACC ACTGCGACAT CTGCCAGCAG 
GATTTCACCT TCACTCCCAC CGAAATCCTC CGCCACAAGA AGCAGCACCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807775.1
CDS	1..3354
Translation	MGMEEEESGSRPRGERARSPPQWDWDHPATRRPLEELYFPRQDPASSQDIPNFWAFFQRLRRFQSRNPHPPEPCPEVATSGPLDLPRGYHPRYRINLSLPRPGRGGDCGIPRDRLAEFRAAFLHYLDFPQKQSFAKLAKLQRERAALPIARYREKLVELVAQHRVVVVAGDTGCGKSTQVPQFLLAAGYSHVACSQPRRIACVALAKRVALESLHQYGSQVGYQIRFESTRSPATRLLFLTEGLLLRQAQRAPALPEYRVLVADEVHERHLQGDVLLGVLRRLLSARPDLRLVLMSATINIPLFSGYFGGAPVLQVPGRIFPISVIYQPIPKEEAPAGGKWGKAERLDPLPYLRVLQAIDHKYPPEERGDLLVFLSGVAEIGAVQEAAQAYATHTQRWIVLPLHSTLSVAEQDKVFDVPPPGVRKCILSTNIAETSVTIDGVRFVLDSGKVKEMSYDPQGKLQRLQEFWISRASAEQRKGRAGRTGPGVCYRLYAESDYDAFAPYPVPEIQRVALDSLVLQLKSMGLGDPRTFPFLEPPPLSSLETALRYLQDQGALDEAEDLTPIGNLLAQLPVDVVVGKMLVLGTLFGLAEPTLTVAAALSVPSPFLRPSHPNPECATARRPLESPHGDPLTLLNLFNEWVQVKSERSGSSRKWCRRRGLEEHRLYEAANLRRQFQELLRDHQLLEEDSGRPSDSYSRQSRHRERRELHRLWRSHTETEGRKRKVLRLQDGADASSGEEEDDGGTRGKGERSIDIQDVKFKLRHNVEELQAVSSSALSSSQLALLKLVLCRGLYPQLAVPDQFNSGRKDSDQIFHTKNKQGVVLHPTCVFATSPELLHAKERRDRGGTGEPTEGLSCHHQLLAFVSLLETNKPYLVNCVRVPALQALLLFSRSLDTSGDCARLVADGWLEVTIPDADSALRLLSTALQLRSSWEKLLHQLLEGRGEELGHHPSPRDVSLLSRGLLDFLQMEVPYRLRQLTGLEKQNLYVGPQTVAAAPRLPGLFQGAEMKPDEVKGGHRVTDFLTYNCLATDTDLYSDCLRSFWTCPHCDLHMPFTPLERMCHQSACRPPEAPLEEPSENSSKTSALHRSYHCDICQQDFTFTPTEILRHKKQHQ

Target ORF information:

RefSeq Version	XM_014952289.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax DEAH (Asp-Glu-Ala-His) box polypeptide 34 (DHX34), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952289.1

ORF Insert Sequence:

ATGGGGATGG AGGAGGAGGA GAGCGGCTCC CGGCCCCGAG GGGAGCGAGC CCGGTCGCCC 
CCCCAGTGGG ACTGGGACCA CCCGGCCACG CGCCGCCCCC TGGAAGAGCT CTATTTCCCC 
CGGCAGGATC CCGCCTCTTC CCAGGATATT CCCAATTTCT GGGCTTTTTT CCAGCGCCTC 
CGACGTTTCC AGAGCCGGAA CCCCCACCCC CCTGAGCCTT GCCCTGAGGT GGCCACCTCT 
GGCCCCCTGG ACTTGCCCCG TGGCTACCAC CCCCGGTACC GCATCAACCT CTCCCTTCCC 
AGGCCGGGGA GGGGGGGGGA TTGTGGCATC CCCCGGGATC GCCTGGCGGA ATTCCGCGCC 
GCTTTCCTCC ACTACCTGGA TTTCCCCCAA AAACAAAGCT TCGCCAAACT GGCCAAACTC 
CAACGGGAAC GAGCCGCGCT GCCCATCGCC CGGTACCGGG AGAAGCTGGT GGAGCTGGTG 
GCCCAGCACC GGGTGGTGGT GGTGGCCGGA GACACCGGCT GCGGCAAATC CACCCAGGTG 
CCCCAGTTCC TGCTGGCGGC CGGTTACAGC CACGTGGCCT GTAGCCAGCC CCGCCGCATC 
GCCTGCGTGG CGCTGGCCAA GAGGGTGGCC CTGGAGAGCC TGCACCAGTA CGGCAGCCAG 
GTGGGCTACC AGATCCGCTT CGAGAGCACG CGCTCCCCGG CCACCCGCCT GCTCTTCCTG 
ACGGAGGGGC TGCTCCTGCG TCAGGCCCAG CGGGCGCCCG CGCTGCCCGA GTACCGCGTC 
CTGGTGGCCG ACGAGGTCCA CGAGCGGCAC CTGCAGGGGG ACGTCCTGCT GGGCGTCCTG 
CGCCGCCTCC TCTCCGCCCG CCCCGACCTC CGCCTGGTCC TCATGTCGGC CACCATCAAC 
ATCCCCCTCT TCTCCGGCTA CTTCGGGGGG GCCCCGGTGC TGCAGGTCCC GGGGAGGATC 
TTCCCCATCT CGGTCATCTA CCAGCCCATC CCTAAGGAAG AGGCCCCGGC GGGTGGGAAA 
TGGGGGAAAG CGGAGCGCCT GGACCCTCTG CCCTACCTGC GGGTGCTTCA GGCCATCGAC 
CACAAGTACC CCCCGGAGGA GCGGGGGGAC CTACTGGTGT TCCTCAGCGG GGTGGCCGAG 
ATCGGGGCGG TGCAGGAGGC GGCCCAGGCT TATGCCACCC ACACCCAGCG CTGGATTGTC 
CTCCCCCTGC ACAGCACCCT CTCGGTGGCT GAGCAGGACA AGGTGTTTGA CGTCCCCCCT 
CCCGGCGTCC GTAAGTGCAT CCTCTCCACC AACATCGCCG AGACCTCGGT GACCATCGAC 
GGCGTCCGCT TTGTCCTGGA TTCGGGGAAG GTGAAGGAGA TGAGTTACGA CCCTCAGGGC 
AAACTCCAGC GGCTGCAGGA GTTCTGGATC AGCCGCGCCA GCGCCGAGCA GCGCAAGGGA 
CGCGCCGGCA GGACCGGCCC CGGCGTCTGC TACCGCCTCT ACGCCGAATC CGACTACGAC 
GCCTTCGCCC CTTACCCCGT GCCGGAGATC CAGCGGGTAG CGCTCGATTC GCTGGTGCTC 
CAGTTAAAGA GTATGGGGCT GGGCGACCCC CGGACCTTCC CTTTTCTGGA GCCCCCACCC 
TTGTCCAGCC TGGAGACAGC CCTGAGGTAC CTGCAGGACC AAGGAGCCCT GGATGAGGCC 
GAAGACCTGA CGCCCATCGG GAACCTGCTG GCCCAGCTGC CGGTAGATGT GGTGGTCGGC 
AAGATGCTGG TCCTGGGCAC CCTCTTTGGC CTGGCTGAGC CCACCCTGAC GGTGGCAGCG 
GCGCTGAGCG TCCCATCCCC CTTCCTGAGA CCCTCTCACC CCAACCCCGA GTGCGCCACG 
GCCCGGCGGC CCCTGGAGAG CCCCCACGGT GACCCCCTGA CTCTGCTCAA CCTCTTCAAC 
GAGTGGGTCC AGGTGAAATC GGAGCGGAGC GGCAGCTCTC GGAAGTGGTG CCGGAGGCGA 
GGTTTGGAGG AGCATCGTCT CTATGAAGCT GCCAACCTTC GGCGCCAGTT CCAGGAGCTT 
CTTCGAGACC ACCAGCTACT GGAGGAAGAT TCTGGTCGCC CCAGCGACAG CTACAGCCGG 
CAGAGCCGGC ACCGGGAACG CCGGGAACTG CACCGGCTCT GGCGATCCCA CACCGAGACC 
GAAGGCCGGA AGCGCAAGGT GCTGCGGCTG CAGGACGGAG CCGATGCCTC CTCCGGCGAG 
GAGGAGGATG ATGGTGGTAC CCGTGGCAAA GGGGAGCGCA GCATCGATAT CCAGGATGTC 
AAGTTCAAGC TCCGCCACAA CGTGGAGGAG CTCCAGGCCG TCTCCAGCTC GGCCCTCTCC 
TCCTCGCAGC TCGCCCTGCT CAAACTGGTG CTGTGCCGGG GGCTTTACCC CCAACTGGCC 
GTCCCTGACC AGTTCAACAG TGGCCGCAAA GACTCCGACC AGATTTTTCA CACCAAAAAC 
AAGCAAGGGG TCGTCCTTCA TCCCACCTGT GTCTTCGCCA CCAGCCCAGA GCTGCTCCAT 
GCCAAGGAGA GACGGGACCG TGGTGGGACT GGAGAGCCCA CAGAGGGGCT GAGCTGCCAC 
CACCAGCTCC TGGCCTTTGT CTCGCTGCTG GAGACCAACA AGCCCTACCT GGTGAACTGT 
GTCCGGGTGC CGGCCCTCCA GGCTCTCCTC CTCTTCTCCC GCTCGCTGGA CACCAGTGGG 
GACTGCGCCC GGCTGGTGGC CGATGGGTGG CTGGAGGTCA CCATCCCCGA CGCGGACTCT 
GCCCTGCGCC TGCTCTCCAC GGCCCTGCAG CTCCGTTCCT CCTGGGAAAA ACTCCTTCAT 
CAGCTGCTGG AAGGTCGAGG AGAGGAACTG GGCCACCATC CCAGCCCCCG TGACGTGTCC 
CTGCTCAGCC GGGGGCTACT GGACTTCCTG CAGATGGAGG TGCCATATCG TCTGCGCCAG 
CTCACGGGGC TGGAGAAACA GAACCTCTAT GTGGGTCCCC AAACGGTGGC GGCTGCTCCC 
CGGCTCCCAG GGCTCTTCCA GGGGGCAGAG ATGAAGCCTG ACGAGGTGAA AGGAGGCCAC 
AGGGTGACGG ATTTTCTCAC CTACAACTGT CTGGCCACGG ACACGGATCT CTACAGCGAC 
TGTCTGAGGA GCTTCTGGAC CTGTCCCCAC TGCGACCTCC ACATGCCCTT CACTCCCCTG 
GAGCGCATGT GCCACCAAAG CGCTTGCCGG CCCCCTGAGG CCCCCCTGGA AGAACCCTCA 
GAAAACTCCT CCAAAACCTC TGCCCTGCAC CGATCCTACC ACTGCGACAT CTGCCAGCAG 
GATTTCACCT TCACTCCCAC CGAAATCCTC CGCCACAAGA AGCAGCACCA GTAA