Custom Monoclonal Antibody Production Services
New Add On Service: Epitope Binning
GenScript has 12-year track record of custom monoclonal antibody production services, generating thousands of successful custom monoclonal antibodies with high-specificity and high-affinity. With our new MonoExpress™ Premium monoclonal antibody production services, you get to test 10 hybridoma supernatants and choose up to 5 clones that actually work for your own applications. No matter your application is Western Blot, IF/flow cytometry, IHC, ICC, IP/ChIP, Sandwich ELISA, or functional assays, we guarantee that at least one clone will work in your own experimental system. Read more »
MonoExpress™ Premium package uses protein as antigen, the gold standard when it comes to high-quality monoclonal antibody production. Protein antigens result in monoclonal antibodies against multiple conformational epitopes of the target protein, maximizing the chances your antibody will work for a variety of applications. To get started, simply provide your target protein sequence; we start with protein antigen production all the way to the production of application guaranteed monoclonal antibodies. MonoExpress™ Gold, Silver, and Bronze monoclonal antibody production services use peptides as antigens and all MonoExpress™ packages come with superior ELISA guarantees. Plus, you'll receive your antibody or hybridoma cell line 4 months faster than traditional immunization protocols.
Key Features of MonoExpress™ Custom Monoclonal Antibody Production Services
- Application-Guaranteed: MonoExpress™ Premium guarantees at least 1 clone will work in your experimental system.
- Optimized immunization: Our advanced technologies - OptimumAntigen™ design tool, proprietary immunization technology, and new GANP mouse – increase specificity and affinity of antibodies.
- Worry-free: Our safety net policy means your mAb investment is protected.
- Fast turnaround time: We deliver antibodies 4 months faster than traditional immunization protocols.
- Certified facility: AAALAC International accreditation and OLAW certification demonstrate our commitment to responsible animal care and use.
MonoExpress™ Custom Monoclonal Antibody Service Packages
Monoclonal antibody production using protein as antigen
Protein antigens are the elite choice when it comes to high-quality monoclonal antibody production. Their unique ability to elicit monoclonal antibodies that recognize the native confirmation of the target protein makes them a superior choice over other antigen strategies. Our popular application-guaranteed MonoExpress™ Premium monoclonal antibody production package exemplifies the high success rate of protein antigens by offering the unprecedented guarantee that at least one clone will work in your own experimental system.
Protein Antigen Packages |
Guarantees |
Starting Material |
Immunization |
Customer Screening |
Deliverables |
First Delivery |
|
|
5 Balb/c mice or 3 rats |
10 ELISA(+) supernatants for evaluation in client's own experimental system* |
|
11 weeks |
|
|
We use your provided protein antigen as the immunogen |
5 Balb/c mice or 3 rats |
5 ELISA(+) supernatants for evaluation in client's own experimental system* |
|
6-7 weeks |
+ Additional clones may be purchased at $399/clone.
Monoclonal antibody production using peptide as antigen
In cases where access to a target protein is limited or unavailable, but the amino acid sequence is known, peptide antigens offer a smart solution for monoclonal antibody production. Peptide antigens are also ideal for producing monoclonal antibodies against post-translational modification sites within a target protein.
Peptide Antigen Packages |
Advantages and Guarantees |
Starting Material |
Immunization |
Deliverables |
Delivery Timeline |
|
|
15 Balb/c mice or 9 rats (5 Balb/c mice or 3 rats per peptide) |
|
13 weeks |
|
|
10 Balb/c mice or 6 rats (5 Balb/c mice or 3 rats per peptide) |
|
|||
|
5 Balb/c mice or 3 rats |
|
++ Purified mAb may be purchased at $399 for 2 mg purified mAb/clone.
What kind of antigen?
Peer reviewed publications using our custom Monoclonal Antibody Services
- Binici J, Hartmann J, Herrmann J, Schreiber C, Beyer S, GÜler G, Vogel V, Tumulka F, Abele R, Mäntele W, Koch J. A soluble fragment of the tumor antigen BCL2-associated athanogene 6 (BAG-6) is essential and sufficient for inhibition of NKp30-dependent cytotoxicity of natural killer cells. J Biol Chem. Oct. 2013; jbc.M113.483602
- Jurisch-Yaksi N, Rose AJ, Lu H, Raemaekers T, Munck S, Baatsen P, Baert V, Vermeire W, Scales SJ, Verleyen D, Vandepoel R, Tylzanowski P, Yaksi E, de Ravel T, Yost HJ, Froyen G, Arrington CB, Annaert W. Rer1p maintains ciliary length and signaling by regulating-secretase activity and Foxj1a level. J Cell Biol. Mar. 2013; 6: 709-720
- Zhu L, Liu D, Li Y, Li N. Functional Phosphoproteomic Analysis Reveals That a Serine-62-Phosphorylated Isoform of Ethylene Response Factor110 Is Involved in Arabidopsis Bolting. Plant Physiol. Feb. 2013; 2: 904-917 Moh MC, Lorenzini PA, Gullo C, Schwarz H. Tumor
- Moh MC, Lorenzini PA, Gullo C, Schwarz H. Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling. FASEB J. Apr. 2013; 27, 2957-2966
- Azevedo MF, Sanders PR, Krejany E, Nie CQ, Fu P, Bach LA, Wunderlich G, Crabb BS, Gilson PR. Inhibition of Plasmodium falciparum CDPK1 by conditional expression of its J-domain demonstrates a key role in schizont development. Biochem J. Jun 2013; 452, 433-441
- Nutabi Camargo, Jos F. Brouwers, Maarten Loos, David H. Gutmann, August B. Smit, and Mark H. G. Verheijen. High-Fat Diet Ameliorates Neurological Deficits Caused By Defective Astrocyte Lipid Metabolism. FASEB J. Oct. 2012; 10, 4302-4315
- Yuan-Yuan Li, Ke Mao, Cheng Zhao, Xian-Yan Zhao, Hua-Lei Zhang, Huai-Rui Shu, and Yu-Jin Hao. Mdcop1 Ubiquitin E3 Ligases Interact With Mdmyb1 To Regulate Light-Induced Anthocyanin Biosynthesis And Red Fruit Coloration In Apple. Plant Physiol. Oct. 2012; 160, 1011-1022
- Lacroix-Pépin N, Danyod G, Krishnaswamy N, Mondal S, Rong PM, Chapdelaine P, Fortier MA. The Multidrug Resistance-Associated Protein 4 (Mrp4) Appears As A Functional Carrier Of Prostaglandins Regulated By Oxytocin In The Bovine Endometrium. Endocrinology. Dec. 2011; 152, 4993-5004
Indirect ELISA screen of hybridoma supernatants generated by MonoExpress™ vs. conventional immunization protocols
Conventional Protocol
The antibody generated by conventional immunization protocol had weak specificity to acetylated peptide antigens and cross-reactivity with corresponding non-acetylated peptide antigens.
MonoExpress™ Protocol
MonoExpress™ antibodies had high specificity to acetylated peptide antigens and no cross-reactivity with corresponding non-acetylated peptide antigens.
Indirect ELISA screen of hybridoma supernatants generated by MonoExpress™ vs. conventional immunization protocols
Conventional Protocol
The antibodies generated by conventional immunization protocol each having a weak binding specificity is shown:
- Clone 1: Recognized dual phosphorylated peptide, single phosphorylated peptide 1, single phosphorylated peptide 2 and non-phosphorylated peptide.
- Clone 2: Recognized dual phosphorylated peptide, single phosphorylated peptide 1 and single phosphorylated peptide 2.
MonoExpress™ Protocol
A panel of monoclonal antibodies each having a unique binding specificity is shown:
- Clone 1: Only recognized dual phosphorylated peptide.
- Clone 2: Only recognized dual phosphorylated peptide.
- Clone 3: Recognized dual phosphorylated peptide and single phosphorylated peptide 1.
- Clone 4: and Clone 5: Recognized dual phosphorylated peptide and single phosphorylated peptide 2.
Affinity analysis of a monoclonal antibody generated by MonoExpress™ Antibody Services
Affinity analysis of a monoclonal antibody generated by MonoExpress™ Antibody Services.
Biacore antibody/antigen affinity analysis showed that the MonoExpress™ generated antibody exhibited an equal or greater affinity to its complementary antigen, compared to an antibody generated by conventional immunization. Typically monoclonal antibodies generated by MonoExpress™ services display reasonably high affinities to their target antigens.
*Read about our safety net policy
If a successful clone cannot be found, customer has the following options:
- Cancel the project after the first delivery of supernatants and only be charged $1,000 (applies to Premium (SC1711) and Basic (SC1717) packages).
- Purchase an additional 10 supernatants for $999. If a successful clone cannot be found after the second supernatant delivery, customer may choose to cancel the project and only be charged $1,000 plus the cost of the additional 10 supernatants, for a total of $1,999 (applies to Premium (SC1711) package only).
For problematic antigens check out our DNA Immunization Service.
If none of these standard packages fit your requirements, we offer fully Customized Monoclonal Antibody Services
For a general overview of our variety of monoclonal antibody services, click here.
Unsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.