![Accelerated Rodent Monoclonal Antibodies]( "Accelerated Rodent Monoclonal Antibodies")
Accelerated Rodent Monoclonal Antibodies
Rapid discovery
Western Blot and ELISA guarantee
Service Specifications
Accelerated (by MonoBoost) and
Classic Antibody Service Packages
Monoclonal antibody production using protein as antigen
Protein antigens are the elite choice when it comes to high-quality monoclonal antibody
production. Their unique ability to elicit monoclonal antibodies that recognize the native
confirmation of the target protein makes them a superior choice over other antigen
strategies. Our popular Accelerated (by MonoBoost) and Classic monoclonal
antibody production package exemplifies the high success rate of protein antigens by
offering the Western blot and superior ELISA titer guarantees!
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Protein Antigen Package
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Guarantee
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Starting Material
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Number of immunized animals
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Customer
Screening
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Deliverables
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First Delivery
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Accelerated By MonoBoost
(SC2129)
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At least 1 WB positive clone for immunogen
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ELISA ≥ 1:64,000
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GenScript generated antigen
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Customer provided antigen
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3
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30 ELISA positive supernatants for initial evaluation by
client
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1 WB positive clone for immunogen
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Up to 5 hybridoma cell lines (2 vials/clone)
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2 mg purified mAb from 1 clone
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0.2 mg purified antigen (if GenScript provides)
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8 wks
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Classic
(SC2128)
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At least 1 clone that is WB positive for immunogen
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ELISA ≥ 1:64,000
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GenScript generated antigen
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Customer provided antigen
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5
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20 ELISA positive supernatants for initial evaluation by
client
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1 WB positive clone for immunogen
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Up to 5 hybridoma cell lines (2 vials/clone)
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2 mg purified mAb from 1 clone
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0.2 mg purified antigen (if GenScript provides)
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18-25 wks
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Note:
* Additional 10 ELISA positive supernatants can be purchased for
$999.
Case Studies
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Indirect ELISA screen of hybridoma supernatants generated by MonoBoost™ vs. conventional immunization protocols
Conventional Protocol
The antibody generated by conventional immunization protocol had weak
specificity to acetylated peptide antigens and cross-reactivity with corresponding
non-acetylated peptide antigens.
MonoBoost™ Protocol
MonoBoost™ antibodies had high specificity to acetylated peptide
antigens and no cross-reactivity with corresponding non-acetylated peptide antigens.
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Indirect ELISA screen of hybridoma supernatants generated by MonoBoost™ vs. conventional immunization protocols
Conventional Protocol
The antibodies generated by conventional immunization
protocol each having a weak binding specificity is shown:
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Clone 1: Recognized dual phosphorylated peptide, single phosphorylated peptide 1, single
phosphorylated peptide 2 and non-phosphorylated peptide.
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Clone 2: Recognized dual phosphorylated peptide, single phosphorylated peptide 1 and
single phosphorylated peptide 2.
MonoBoost™ Protocol
A panel of monoclonal antibodies each having a unique
binding specificity is shown:
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Clone 1: Only recognized dual phosphorylated peptide.
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Clone 2: Only recognized dual phosphorylated peptide.
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Clone 3: Recognized dual phosphorylated peptide and single phosphorylated peptide 1.
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Clone 4: and Clone 5: Recognized dual phosphorylated peptide and single phosphorylated
peptide 2.
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Affinity analysis of a monoclonal antibody generated by MonoBoost™ Antibody Services
Affinity analysis of a monoclonal antibody generated by MonoBoost™
Antibody Services.
Biacore antibody/antigen affinity analysis showed that the
MonoBoost™ generated antibody exhibited an equal or greater affinity to its
complementary antigen, compared to an antibody generated by conventional immunization.
Typically monoclonal antibodies generated by MonoBoost™ services display reasonably
high affinities to their target antigens.
Get in Touch
with GenScript Custom
Antibody Services
Publications
Peer reviewed publications using our custom Monoclonal Antibody Services
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Binici J, Hartmann J, Herrmann J, Schreiber C, Beyer S, GÜler G, Vogel V, Tumulka F,
Abele R, Mäntele W, Koch J.
A soluble fragment of the tumor antigen BCL2-associated athanogene 6 (BAG-6) is
essential and sufficient for inhibition of NKp30-dependent cytotoxicity of natural
killer cells. J Biol Chem. Oct. 2013; jbc.M113.483602
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Jurisch-Yaksi N, Rose AJ, Lu H, Raemaekers T, Munck S, Baatsen P, Baert V, Vermeire W,
Scales SJ, Verleyen D, Vandepoel R, Tylzanowski P, Yaksi E, de Ravel T, Yost HJ, Froyen
G, Arrington CB, Annaert W.
Rer1p maintains ciliary length and signaling by regulating-secretase activity and
Foxj1a level. J Cell Biol. Mar. 2013; 6: 709-720
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Zhu L, Liu D, Li Y, Li N. Functional
Phosphoproteomic Analysis Reveals That a Serine-62-Phosphorylated Isoform of Ethylene
Response Factor110 Is Involved in Arabidopsis Bolting. Plant Physiol. Feb.
2013; 2: 904-917 Moh MC, Lorenzini PA, Gullo C, Schwarz H. Tumor
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Moh MC, Lorenzini PA, Gullo C, Schwarz H. Tumor
necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse
signaling. FASEB J. Apr. 2013; 27, 2957-2966
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Azevedo MF, Sanders PR, Krejany E, Nie CQ, Fu P, Bach LA, Wunderlich G, Crabb BS, Gilson
PR.
Inhibition
of Plasmodium falciparum CDPK1 by conditional expression of its J-domain
demonstrates a key role in schizont development. Biochem J. Jun 2013;
452, 433-441
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Nutabi Camargo, Jos F. Brouwers, Maarten Loos, David H. Gutmann, August B. Smit, and
Mark H. G. Verheijen. High-Fat
Diet Ameliorates Neurological Deficits Caused By Defective Astrocyte Lipid
Metabolism. FASEB J. Oct. 2012; 10, 4302-4315
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Yuan-Yuan Li, Ke Mao, Cheng Zhao, Xian-Yan Zhao, Hua-Lei Zhang, Huai-Rui Shu, and Yu-Jin
Hao.
Mdcop1 Ubiquitin E3 Ligases Interact With Mdmyb1 To Regulate Light-Induced
Anthocyanin Biosynthesis And Red Fruit Coloration In Apple. Plant
Physiol. Oct. 2012; 160, 1011-1022
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Lacroix-Pépin N, Danyod G, Krishnaswamy N, Mondal S, Rong PM, Chapdelaine P, Fortier MA.
The
Multidrug Resistance-Associated Protein 4 (Mrp4) Appears As A Functional Carrier Of
Prostaglandins Regulated By Oxytocin In The Bovine Endometrium. Endocrinology.
Dec. 2011; 152, 4993-5004
*Read about our safety net policy
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Level 1: If customer cancels project after first supernatant delivery, 50% of package price is charged
(excludes antigen preparation cost if GenScript provides).
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Level 2: If customer cancels after ordering additional 10 ELISA (+) supernatants, 50% of package price +
cost of additional supernatants is charged.
For problematic antigens check out our DNA Immunization Service.
If none of these standard packages fit your requirements, we offer fully Customized Monoclonal Antibody
Services.
For a general overview of our variety of monoclonal antibody services, click here.
Unsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.
Quotation and Ordering
Our customer service representatives are available 24 hours Monday through Friday. You may contact
us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment
information (credit card, purchase order or bank information) ready.
A Technical Account Manager will
reply you within 24 hours to finalize the quote
To place order, provide credit
card/PO information
Production of your project begins
immediately
A Project Manager is in contact
with you during the production process to give updates
