GenScript has 15-year track record of custom monoclonal antibody production services, generating thousands of successful custom monoclonal antibodies (mAbs) with high specificity and high affinity. With our MonoExpress™ Basic and Premium mAb production services, you get to test 5 to 10 hybridoma supernatants in your in-house research application, and choose up to 5 hybridoma cell lines for final delivery. With these packages, we guarantee that the mAbs delivered are Western blot positive for your protein antigen, and have an ELISA titer ≥ 1:64,000. Plus, you'll receive your antibody or hybridoma cell line 4 months faster than traditional immunization protocols! Read more »
The MonoExpress™ Basic and Premium Packages use protein as the antigen because it is the gold standard when it comes to high-quality mAb production. Protein antigens result in mAbs that target multiple conformational epitopes of the target protein, maximizing the chances your antibody will work for a variety of applications. Need help expressing your protein antigen? We can do it for you with our MonoExpress™ Premium Package! To get started, simply provide us with your target protein sequence; we will start with protein antigen production all the way to the monoclonal antibody generation. All MonoExpress™ packages come with Western blot and ELISA guarantees.
Key Features of MonoExpress™ Custom Monoclonal Antibody Production Services
- Application Guarantees: Our MonoExpress™ Premium and Basic packages guarantee that the mAb clones delivered are Western blot positive for your protein immunogen and possess an ELISA titer ≥ 1:64,000.
- Optimized immunization: Our advanced technologies - OptimumAntigen design tool, proprietary immunization technology – increase specificity and affinity of antibodies.
- Worry-free: Our safety net policy means your mAb investment is protected.
- Fast turnaround time: We deliver antibodies 4 months faster than traditional immunization protocols.
- Certified facility: AAALAC International accreditation and OLAW certification demonstrate our commitment to responsible animal care and use.
MonoExpress™ Custom Monoclonal Antibody Service Packages
Monoclonal antibody production using protein as antigen
Protein antigens are the elite choice when it comes to high-quality monoclonal antibody production. Their unique ability to elicit monoclonal antibodies that recognize the native confirmation of the target protein makes them a superior choice over other antigen strategies. Our popular MonoExpress™ Basic and Premium monoclonal antibody production package exemplifies the high success rate of protein antigens by offering the Western blot and superior ELISA titer guarantees!
Protein Antigen Packages |
Guarantees |
Starting Material |
Immunization |
Customer Screening |
Deliverables |
First Delivery |
|
|
5 Balb/c mice or 3 rats |
10 ELISA(+) supernatants for evaluation in client's own experimental system* |
|
11 weeks |
|
|
We use your provided protein antigen as the immunogen |
5 Balb/c mice or 3 rats |
5 ELISA(+) supernatants for evaluation in client's own experimental system* |
|
6-7 weeks |
+ Additional clones may be purchased at $399/clone.
Peer reviewed publications using our custom Monoclonal Antibody Services
- Binici J, Hartmann J, Herrmann J, Schreiber C, Beyer S, GÜler G, Vogel V, Tumulka F, Abele R, Mäntele W, Koch J. A soluble fragment of the tumor antigen BCL2-associated athanogene 6 (BAG-6) is essential and sufficient for inhibition of NKp30-dependent cytotoxicity of natural killer cells. J Biol Chem. Oct. 2013; jbc.M113.483602
- Jurisch-Yaksi N, Rose AJ, Lu H, Raemaekers T, Munck S, Baatsen P, Baert V, Vermeire W, Scales SJ, Verleyen D, Vandepoel R, Tylzanowski P, Yaksi E, de Ravel T, Yost HJ, Froyen G, Arrington CB, Annaert W. Rer1p maintains ciliary length and signaling by regulating-secretase activity and Foxj1a level. J Cell Biol. Mar. 2013; 6: 709-720
- Zhu L, Liu D, Li Y, Li N. Functional Phosphoproteomic Analysis Reveals That a Serine-62-Phosphorylated Isoform of Ethylene Response Factor110 Is Involved in Arabidopsis Bolting. Plant Physiol. Feb. 2013; 2: 904-917 Moh MC, Lorenzini PA, Gullo C, Schwarz H. Tumor
- Moh MC, Lorenzini PA, Gullo C, Schwarz H. Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling. FASEB J. Apr. 2013; 27, 2957-2966
- Azevedo MF, Sanders PR, Krejany E, Nie CQ, Fu P, Bach LA, Wunderlich G, Crabb BS, Gilson PR. Inhibition of Plasmodium falciparum CDPK1 by conditional expression of its J-domain demonstrates a key role in schizont development. Biochem J. Jun 2013; 452, 433-441
- Nutabi Camargo, Jos F. Brouwers, Maarten Loos, David H. Gutmann, August B. Smit, and Mark H. G. Verheijen. High-Fat Diet Ameliorates Neurological Deficits Caused By Defective Astrocyte Lipid Metabolism. FASEB J. Oct. 2012; 10, 4302-4315
- Yuan-Yuan Li, Ke Mao, Cheng Zhao, Xian-Yan Zhao, Hua-Lei Zhang, Huai-Rui Shu, and Yu-Jin Hao. Mdcop1 Ubiquitin E3 Ligases Interact With Mdmyb1 To Regulate Light-Induced Anthocyanin Biosynthesis And Red Fruit Coloration In Apple. Plant Physiol. Oct. 2012; 160, 1011-1022
- Lacroix-Pépin N, Danyod G, Krishnaswamy N, Mondal S, Rong PM, Chapdelaine P, Fortier MA. The Multidrug Resistance-Associated Protein 4 (Mrp4) Appears As A Functional Carrier Of Prostaglandins Regulated By Oxytocin In The Bovine Endometrium. Endocrinology. Dec. 2011; 152, 4993-5004
Indirect ELISA screen of hybridoma supernatants generated by MonoExpress™ vs. conventional immunization protocols
Conventional Protocol
The antibody generated by conventional immunization protocol had weak specificity to acetylated peptide antigens and cross-reactivity with corresponding non-acetylated peptide antigens.
MonoExpress™ Protocol
MonoExpress™ antibodies had high specificity to acetylated peptide antigens and no cross-reactivity with corresponding non-acetylated peptide antigens.
Indirect ELISA screen of hybridoma supernatants generated by MonoExpress™ vs. conventional immunization protocols
Conventional Protocol
The antibodies generated by conventional immunization protocol each having a weak binding specificity is shown:
- Clone 1: Recognized dual phosphorylated peptide, single phosphorylated peptide 1, single phosphorylated peptide 2 and non-phosphorylated peptide.
- Clone 2: Recognized dual phosphorylated peptide, single phosphorylated peptide 1 and single phosphorylated peptide 2.
MonoExpress™ Protocol
A panel of monoclonal antibodies each having a unique binding specificity is shown:
- Clone 1: Only recognized dual phosphorylated peptide.
- Clone 2: Only recognized dual phosphorylated peptide.
- Clone 3: Recognized dual phosphorylated peptide and single phosphorylated peptide 1.
- Clone 4: and Clone 5: Recognized dual phosphorylated peptide and single phosphorylated peptide 2.
Affinity analysis of a monoclonal antibody generated by MonoExpress™ Antibody Services
Affinity analysis of a monoclonal antibody generated by MonoExpress™ Antibody Services.
Biacore antibody/antigen affinity analysis showed that the MonoExpress™ generated antibody exhibited an equal or greater affinity to its complementary antigen, compared to an antibody generated by conventional immunization. Typically monoclonal antibodies generated by MonoExpress™ services display reasonably high affinities to their target antigens.
*Read about our safety net policy
If a successful clone cannot be found, customer has the following options:
- Cancel the project after the first delivery of supernatants and only be charged $1899 for basic (SC1717) and $2999 for premium (SC1711) packages.
- Purchase an additional 10 supernatants for $999. If a successful clone cannot be found after the second supernatant delivery, customer may choose to cancel the project and only be charged the $2999 plus the cost of the additional 10 supernatants, for a total of $3998 (applies to premium (SC1711) package only).
For problematic antigens check out our DNA Immunization Service.
If none of these standard packages fit your requirements, we offer fully Customized Monoclonal Antibody Services.
For a general overview of our variety of monoclonal antibody services, click here.
Unsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
