MonoBoost™-Fast Immunization Protocol for Mouse

Case Studies

• Indirect ELISA screen of hybridoma supernatants generated by MonoBoost™ vs. conventional immunization protocols

  Conventional Protocol

  

  The antibody generated by conventional immunization protocol had weak specificity to acetylated peptide antigens and cross-reactivity with corresponding non-acetylated peptide antigens.

  MonoBoost™ Protocol

  

  MonoBoost™ antibodies had high specificity to acetylated peptide antigens and no cross-reactivity with corresponding non-acetylated peptide antigens.

• Indirect ELISA screen of hybridoma supernatants generated by MonoBoost™ vs. conventional immunization protocols

  Conventional Protocol

  

  The antibodies generated by conventional immunization protocol each having a weak binding specificity is shown:

  • Clone 1: Recognized dual phosphorylated peptide, single phosphorylated peptide 1, single phosphorylated peptide 2 and non-phosphorylated peptide.
  • Clone 2: Recognized dual phosphorylated peptide, single phosphorylated peptide 1 and single phosphorylated peptide 2.

  MonoBoost™ Protocol

  

  A panel of monoclonal antibodies each having a unique binding specificity is shown:

  • Clone 1: Only recognized dual phosphorylated peptide.
  • Clone 2: Only recognized dual phosphorylated peptide.
  • Clone 3: Recognized dual phosphorylated peptide and single phosphorylated peptide 1.
  • Clone 4: and Clone 5: Recognized dual phosphorylated peptide and single phosphorylated peptide 2.
• Affinity analysis of a monoclonal antibody generated by MonoBoost™ Antibody Services
  

  Affinity analysis of a monoclonal antibody generated by MonoBoost™ Antibody Services.
  
  Biacore antibody/antigen affinity analysis showed that the MonoBoost™ generated antibody exhibited an equal or greater affinity to its complementary antigen, compared to an antibody generated by conventional immunization. Typically monoclonal antibodies generated by MonoBoost™ services display reasonably high affinities to their target antigens.

Publications

Peer reviewed publications using our custom Monoclonal Antibody Services

• Binici J, Hartmann J, Herrmann J, Schreiber C, Beyer S, GÜler G, Vogel V, Tumulka F, Abele R, Mäntele W, Koch J. A soluble fragment of the tumor antigen BCL2-associated athanogene 6 (BAG-6) is essential and sufficient for inhibition of NKp30-dependent cytotoxicity of natural killer cells. J Biol Chem. Oct. 2013; jbc.M113.483602
• Jurisch-Yaksi N, Rose AJ, Lu H, Raemaekers T, Munck S, Baatsen P, Baert V, Vermeire W, Scales SJ, Verleyen D, Vandepoel R, Tylzanowski P, Yaksi E, de Ravel T, Yost HJ, Froyen G, Arrington CB, Annaert W. Rer1p maintains ciliary length and signaling by regulating-secretase activity and Foxj1a level. J Cell Biol. Mar. 2013; 6: 709-720
• Zhu L, Liu D, Li Y, Li N. Functional Phosphoproteomic Analysis Reveals That a Serine-62-Phosphorylated Isoform of Ethylene Response Factor110 Is Involved in Arabidopsis Bolting. Plant Physiol. Feb. 2013; 2: 904-917 Moh MC, Lorenzini PA, Gullo C, Schwarz H. Tumor
• Moh MC, Lorenzini PA, Gullo C, Schwarz H. Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling. FASEB J. Apr. 2013; 27, 2957-2966
• Azevedo MF, Sanders PR, Krejany E, Nie CQ, Fu P, Bach LA, Wunderlich G, Crabb BS, Gilson PR. Inhibition of Plasmodium falciparum CDPK1 by conditional expression of its J-domain demonstrates a key role in schizont development. Biochem J. Jun 2013; 452, 433-441
• Nutabi Camargo, Jos F. Brouwers, Maarten Loos, David H. Gutmann, August B. Smit, and Mark H. G. Verheijen. High-Fat Diet Ameliorates Neurological Deficits Caused By Defective Astrocyte Lipid Metabolism. FASEB J. Oct. 2012; 10, 4302-4315
• Yuan-Yuan Li, Ke Mao, Cheng Zhao, Xian-Yan Zhao, Hua-Lei Zhang, Huai-Rui Shu, and Yu-Jin Hao. Mdcop1 Ubiquitin E3 Ligases Interact With Mdmyb1 To Regulate Light-Induced Anthocyanin Biosynthesis And Red Fruit Coloration In Apple. Plant Physiol. Oct. 2012; 160, 1011-1022
• Lacroix-Pépin N, Danyod G, Krishnaswamy N, Mondal S, Rong PM, Chapdelaine P, Fortier MA. The Multidrug Resistance-Associated Protein 4 (Mrp4) Appears As A Functional Carrier Of Prostaglandins Regulated By Oxytocin In The Bovine Endometrium. Endocrinology. Dec. 2011; 152, 4993-5004

*Read about our safety net policy

• Level 1: If customer cancels project after first supernatant delivery, 50% of package price is charged (excludes antigen preparation cost if GenScript provides).
• Level 2: If customer cancels after ordering additional 10 ELISA (+) supernatants, 50% of package price + cost of additional supernatants is charged.

For problematic antigens check out our DNA Immunization Service.

If none of these standard packages fit your requirements, we offer fully Customized Monoclonal Antibody Services.

For a general overview of our variety of monoclonal antibody services, click here.

Unsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.

Quotation and Ordering

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.