Ascites Production for In Vivo Antibody Scale-Up
GenScript’s ascites production service is available in nude and Balb/c mice, with a turnaround time of just 5 – 7 weeks. While the amount of purified antibodies obtained from 5 mice typically ranges between 5 – 50 mg, yield is greatly dependent on the productivity of each individual hybridoma cell line.
| Cat. No. | Service Package Name | Price |
|---|---|---|
| SC2073 | Ascites Production in 5 Balb/c mice | $805 |
| Low endotoxin ascites Production in 5 Balb/c mice | $1,210 | |
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| Description | Time Line | |
|---|---|---|
| Phase I: Cell line submission, recovery, and expansion |
Customer submits:
After the materials and information listed above have been received, the hybridoma cell line will be recovered and examined for contamination. If no contamination is detected, the cell line will be expanded to generate sufficient cells for mouse inoculation. |
1-2 weeks
|
| Phase II: Inoculation |
Hybridoma cells will be injected into the mouse peritoneal cavity to commence ascites production. |
2 – 3 weeks
|
| Phase III: Harvest |
Ascites fluids are harvested and clarified by centrifugation. Clarified ascites are then aliquoted and immediately frozen or purified. |
1 week
|
Related Antibody Production Services
In addition to in vivo antibody scale up production using ascites fluid, GenScript also provides in vitro solutions including:
- Hybridoma-based antibody production: High purity (≥ 90%) monoclonal antibody production at flexible scales, with serum free & low endotoxin (≤ 3 EU/mg) service options.
- Recombinant monoclonal antibody production: Recombinant mAb production at flexible scales using GenScript’s proprietary High Density (HD) Transient Expression System. See yield improvements of up to 100-fold and antibodies delivered in as little as 10 weeks. Guaranteed service options available!
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
