Independent Antibody Validation

Epitope Binning is a means of antibody validation in which monoclonal antibodies are tested against one another to determine which antibodies compete for the same antigenic epitope. Antibodies which bind to identical epitopes are grouped together into "bins". Antibodies which are grouped into different bins are considered to be independent from one another, hence the name independent antibody validation (Figure 1). Independent antibody validation is used to identify which antibody pairs have higher specificity and/or sensitivity in various experimental platforms.

Independent Antibody Pairs

Figure 1. Independent Antibody Pairs. (A) An antigen can have multiple epitopes in which an antibody can bind. (B) If two mAbs bind to same epitope on an antigen, they are considered to be in one bin. (C) However, if two mAbs both bind to the same antigen, but at different epitopes, they are considered to be in separate bins.

Key Features of GenScript's Independent Antibody Validation Service

number 1
Final Delivery: An epitope binning test report identifying all "independent pairs"
number 2
Can be added to custom mAb generation services including Lead generation and optimization
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Can be performed on mAb's generated against
protein antigens
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Can be performed on up to 5 mAbs
at one time
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Can be performed between different groups of
5 mAbs

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