After GenScript performs epitope binning on the customer selected purified mAbs, you will receive an epitope binning report explaining which purified mAbs belong to which epitope bin. An example report is shown in table 1. All biotin labeled testing mAbs will be shown on the top of the table, while the competing mAbs will be labeled on the left side of the table. The numerical results of the direct ELISA evaluating the competition between each testing and competing antibody is shown in the corresponding section within the table. It is important to remember, that only the bound biotin labeled testing antibody was measured, therefore a low percent binding number indicates that the test mAb was unable to bind when in the presence of the competing mAb, indicating that they both bind to the same antigen and therefore in the same bin.
| Percent binding |
mAb I-biotin |
mAb II-biotin |
mAb III-biotin |
mAb IV-biotin |
| No competing Ab |
100 |
100 |
100 |
100 |
| mAb I competing |
10.3 |
8.8 |
18.6 |
142.7 |
| mAb II competing |
7.6 |
7.7 |
8.6 |
134.8 |
| mAb III competing |
10.1 |
9.2 |
11.2 |
132 |
| mAb IV competing |
93.2 |
106.2 |
108 |
19.8 |
Table 1. Example of Epitope Binning Report. The example epitope binning report contains the percentage of testing mAb which binds to a specific epitope when a competing mAb is present. The above table indicates that mAb I, mAb II, and mAb III belong to the same bin and are independent from mAb IV.