Goal: Generate rabbit monoclonal antibodies that are specific to Claudin 18.2 but not recognizing Claudin 18.1
Immunogen: DNA + Protein
Result: Successfully generated 47 Claudin 18.2-positive and Claudin 18.1-negative clones; among these clone 8 performed well in IF and IHC assays.

Screening Methods | Indirect ELISA | Cell-Based ELISA | Cell-Based IHC | FACS | FACS |
---|---|---|---|---|---|
Clone ID | Claudin 18.2 protein | Vero-Claudin18.2 | Vero-Claudin 18.2 | CHO-K1-Claudin 18.1 | |
Clone 1 | 2.631 | 1.39 | 0.67 | + | - |
Clone 2 | 2.537 | 1.304 | 0.657 | + | - |
Clone 3 | 2.651 | 1.523 | 1.023 | + | - |
Clone 4 | 2.588 | 1.529 | 1.18 | + | - |
Clone 5 | 2.564 | 1.585 | 1.207 | + | - |
Clone 6 | 2.498 | 0.575 | 0.575 | + | - |
Clone 7 | 2.305 | 0.503 | 0.763 | + | - |
Clone 8 | 2.475 | 1.437 | 1.082 | + | - |
IF Results of Clone 8

The fluorescent staining could be observed on the Vero-18.2 samples for both supernatant and purified antibody of the Clone 8.
IHC Results of Clone 8

The membrane staining on paraffin-embedded tissue sections of gastric cancer could be observed.